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Microbial thiamin metabolism in the rumen simulating fermenter (RUSITEC): the effect of acidogenic conditions, a high sulfur level and added thiamin

Published online by Cambridge University Press:  09 March 2007

L. Alves De Oliveira ,
C. Jean-Blain ,
S. Komisarczuk-Bony ,
A. Durix  and
C. Durier
L. Alves De Oliveira
Affiliation:
Equipe associée INRA de Physiopathologie du rumen, Ecole Nationale Vétérinaire de Lyon, BP 83, 69280 Marcy l'Etoile, France
C. Jean-Blain
Affiliation:
Equipe associée INRA de Physiopathologie du rumen, Ecole Nationale Vétérinaire de Lyon, BP 83, 69280 Marcy l'Etoile, France
S. Komisarczuk-Bony
Affiliation:
Equipe associée INRA de Physiopathologie du rumen, Ecole Nationale Vétérinaire de Lyon, BP 83, 69280 Marcy l'Etoile, France
A. Durix
Affiliation:
Equipe associée INRA de Physiopathologie du rumen, Ecole Nationale Vétérinaire de Lyon, BP 83, 69280 Marcy l'Etoile, France
C. Durier
Affiliation:
Laboratoire de Biométrie INRA, Route de St Cyr – 78026 Versailles Cedex, France
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Abstract

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The effects of acidogenic conditions, a high S level and the addition of thiamin on the rumen microbial metabolism of thiamin were investigated in vitro in a semi-continuous fermenter (RUSITEC), using a factorial design. Acidogenic conditions were obtained by simultaneously increasing the starch: cellulose ratio and the amount of solid substrate fed, and by decreasing the buffering capacity of the liquid phase of the fermenter. S in the form of sulfate was supplied at two levels, one corresponding to a control amount of S (2 g/kg dietary DM), the second to an excess (5 g/ kg DM) which is sufficient to trigger cerebrocortical necrosis (CCN) when used in vivo. Acidogenic conditions decreased the pH of the fermenters, CH4 production and cellulose digestibility, increased the short-chain fatty acid production, but had no effect on thiamin production. The high S level enhanced the production of sulfide considerably, had no effect on the microbial metabolism of energy and N, and decreased thiamin production (326 ν. 266 nmol/d). The added thiamin was rapidly converted into phosphorylated compounds which largely decreased the apparent synthesis of this vitamin by the rumen microflora. The total thiamin flow was increased by added thiamin. In no case was thiaminase activity in the fermenter liquid phase significantly modified. The high level of S induced only a limited decrease of total thiamin flow. Consequently, it is unlikely that the investigated factors could be considered to be high risk factors for the thiamin-dependent CCN.

Keywords

ThiaminSulfur excessCerebrocortical necrosis
Type
Animal Nutrition
Information
British Journal of Nutrition , Volume 78 , Issue 4 , October 1997 , pp. 599 - 613
Copyright
Copyright © The Nutrition Society 1997

References

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