Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-25T01:05:02.486Z Has data issue: false hasContentIssue false
  • English
  • Français

Metabolites of riboflavine in milk, urine and tissues of animals in relation to alimentary symbiotic bacteria

Published online by Cambridge University Press:  09 March 2007

E. C. Owen  and
D. W. West
E. C. Owen
Affiliation:
Biochemistry Department, Hannah Dairy Research Institute, Ayr
D. W. West
Affiliation:
Biochemistry Department, Hannah Dairy Research Institute, Ayr
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Riboflavine given orally to sheep, calves, goats and ruminating kids gave rise, in milk and urine, to extra riboflavine accompanied by hydroxyethylflavine, by formylmethylflavine and by other as yet unidentified metabolites, but subcutaneous injection of riboflavine caused prolonged excretion of riboflavine in urine and milk unaccompanied by metabolites.

2. Placement of riboflavine by means of fistulas into the rumen, the abomasum or the caecum and abstraction of samples from the rumen or caecum showed that the rumen was the site of origin of the metabolites, thus accounting for the lapse of time of several hours between the ingestion of riboflavine and the appearance of hydroxyethylflavine in the urine and the absence of metabolites after subcutaneous injection of riboflavine.

3. Kids in which rumination had not yet become established could not produce metabolites from ingested riboflavine although, when fed milk containing the metabolites, they readily excreted them in their urine.

5. Incubation in vitro of kidney or liver tissue from cattle or goats with riboflavine did not give rise to metabolites, but these tissues could both reduce formylmethylflavine to hydroxyethylflavine in vitro.

6. Post-mortem examination of animals to which riboflavine had been fed showed the presence of metabolites only in gut contents, in the urinary bladder and in the bile. When injected, riboflavine reached the bile but no metabolites were produced.

7. Metabolites were found to occur naturally in the urine of ruminants in small amounts. Hydroxyethylflavine was readily demonstrable in the urine of the rabbit, but in the urine of the dog metabolites could not be found.

8. From these and earlier and later experiments the conclusion is drawn that riboflavine metabolites arise in the ruminant from the degradation of riboflavine by rumen bacteria.

Type
Research Article
Information
British Journal of Nutrition , Volume 24 , Issue 1 , March 1970 , pp. 45 - 60
Copyright
Copyright © The Nutrition Society 1970

References

Bessey, O. A., Lowry, O. H. & Love, R. H. (1949). J. biol. Chem. 180, 755.CrossRefGoogle Scholar
Crammer, J. L. (1948). Nature, Lond. 161, 349.Google Scholar
Cresswell, R. M. & Wood, H. C. S. (1960). J. chem. Soc. p. 4768.CrossRefGoogle Scholar
Crossland, A., Owen, E. C. & Proudfoot, R. (1958). Br. J. Nutr. 12, 312.CrossRefGoogle Scholar
Edwards, D. C. & Darroch, R. A. (1956). Br. J. Nutr. 10, 286.CrossRefGoogle Scholar
Faitelberg, R. O. & Semenjuk, L. A. (1956). Byull. eksp. Biol. Med. 41, 23.CrossRefGoogle Scholar
Fall, H. H. & Petering, H. G. (1956). J. Am. chem. Soc. 78, 377.CrossRefGoogle Scholar
Fox, H. C. & Miller, D. S. (1960). Nature, Lond. 186, 561.CrossRefGoogle Scholar
Hobson, P. N., Summers, R., Owen, E. C., Spencer, J. C. & West, D. W. (1969). Proc. Nutr. Soc. 28, 53A.Google Scholar
Lane, M., Fahey, J. L., Sullivan, R. D. & Zubrod, C. G. (1958). J. Pharmac. exp. Ther. 122, 315.Google Scholar
Lane, M., Petering, H. G. & Brindley, C. O. (1959). J. natn. Cancer Inst. 22, 349.Google Scholar
McDougall, E. I. (1948). Biochem. J. 43, 99.CrossRefGoogle Scholar
Maslenikova, E. M. (1962). Vop. Pitan. 21, 56.Google Scholar
Miller, R. F., Price, N. O. & Engel, R. W. (1956). J. Nutr. 60, 539.CrossRefGoogle Scholar
Owen, E. C. (1954). J. Dairy Res. 21, 408.CrossRefGoogle Scholar
Owen, E. C. (1959). Revue Path. gén. Physiol. clin. 59, 234.Google Scholar
Owen, E. C. (1962 a). Proc. int. Congr. Fd Sci. I. London. Vol. 3, p. 669.Google Scholar
Owen, E. C. (1962 b). Biochem. J. 84, 96P.Google Scholar
Owen, E. C. (1967). In Urea as a Protein Supplement. Ch. 17. [Briggs, H. M., editor.] London: Pergamon Press.Google Scholar
Owen, E. C. (1968). Lab. Pract. p. 1137.Google Scholar
Owen, E. C. & Dzialoszynski, L. (1965 a). Proc. Nutr. Soc. 24, ix.Google Scholar
Owen, E. C. & Dzialoszynski, L. (1965 b). Proc. Nutr. Soc. 24, ix.Google Scholar
Owen, E. C., Montgomery, J. P. & Proudfoot, R. (1962). Biochem. J. 82, 8P.Google Scholar
Owen, E. C. & West, D. W. (1968 a). J. chem. Soc. C, p. 34.Google Scholar
Owen, E. C. & West, D. W. (1968 b). Chemy Ind. p. 881.Google Scholar
Owen, E. C., West, D. W. & Coates, M. E. (1970). Br. J. Nutr. 24, 259.CrossRefGoogle Scholar
Sone, K. (1958). Tohoku J. agric. Res. 9, 93.Google Scholar
Virtanen, A. I. (1967). In Ureua as a Protein Supplement. Ch. 10. [Briggs, H. M., editor.] London: Pergamon Press.Google Scholar
Wattiaux, R., Wattiaux-de Conink, S., Rutgeerts, M. J. & Tulkens, P. (1964). Nature, Lond. 203, 757.CrossRefGoogle Scholar
West, D. W. & Owen, E. C. (1968). Proc. Nutr. Soc. 27, 39A.Google Scholar
West, D. W. & Owen, E. C. (1969). Br. J. Nutr. 23, 889.CrossRefGoogle Scholar
West, D. W., Owen, E. C. & Taylor, M. M. (1967). Proc. Nutr. Soc. 26, xvii.Google Scholar