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Metabolism of parenterally administered fat emulsions in the rat: studies of fatty acid oxidation with 1-13C- and 8-13C-labelled triolein

Published online by Cambridge University Press:  09 March 2007

Wolfgang Bäurle ,
Herbert Brösicke ,
Dwight E. Matthews ,
Karin Pogan  and
Peter Fürst
Wolfgang Bäurle
Affiliation:
Institute for Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany
Herbert Brösicke
Affiliation:
GCMS-Laboratory, Children's Hospital of the Free University of Berlin, Heubnerweg 16, 14059 Berlin, Germany
Dwight E. Matthews
Affiliation:
Department of Medicine, The University of Vermont College of Medicine, Given Building B217, Endo, Burlington, VT 05405, USA
Karin Pogan
Affiliation:
Institute for Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany
Peter Fürst*
Affiliation:
Institute for Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany
*
*Corresponding author:Prof. Peter Fürst, fax +49 711 4592283, email [email protected]
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Abstract

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To reassess the hypothesis that fatty acid catabolism occurs to completion via β-oxidation, male Sprague–Dawley rats receiving continuous total parenteral nutrition (TPN) including 43% energy as fat were infused with [1-13C]- or [8-13C]triolein. Expired CO2 was collected continuously for 4 h and its 13C: 12C ratio determined by isotope–ratio mass spectrometry. Bicarbonate retention was also assessed over 4 h by infusion of NaH14CO3 and measurement of the expired 14CO2. A possible loss of label from [8-13C]oleic acid from the citric acid cycle via labelled acetyl-CoA without oxidation to CO2 was assessed by infusing further animals with acetate labelled with 14C either at C atoms 1 or 2 and determination of its conversion to expired 14CO2. At isotopic steady state, 63.2 (SE 1.6)% (n 8) of the infused [1-14C]acetate and 46.0 (SE 1.2)% (n 8) of [2-14C]acetate was recovered as expired 14CO2. After correction for bicarbonate retention and non-oxidative isotope loss, 37.3 (SE 1.2)% (n 20) of the [1-13C]triolein was found to have been oxidized, whereas 32.6 (SE 1.0)% (n 20) of the [8-13C]triolein was oxidized (P ≤ 0.01). The lower oxidation of the C atom at position 8 of oleic acid than that at position 1 indicates incomplete oxidative breakdown of the fatty acid after entering β-oxidation.

Keywords

13C-labelled trioleinβ-oxidationTotal parenteral nutrition
Type
General Nutrition
Information
British Journal of Nutrition , Volume 79 , Issue 4 , April 1998 , pp. 381 - 387
Copyright
Copyright © The Nutrition Society 1998

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