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Metabolic effects of trans fatty acids on an experimental dietary model

Published online by Cambridge University Press:  09 March 2007

María E. Colandré ,
Rosaura S. Diez  and
Claudio A. Bernal
María E. Colandré
Affiliation:
Cátedra Bromatología y Nutrición, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina
Rosaura S. Diez
Affiliation:
Cátedra Bromatología y Nutrición, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina
Claudio A. Bernal*
Affiliation:
Cátedra Bromatología y Nutrición, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, Argentina
*
*Corresponding Author: Professor Claudio Bernal, fax +54 342 4575221, email [email protected]
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Abstract

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The aim of the present study was to investigate the potential nutritional and metabolic impact of trans (t) fatty acids (FA) on an appropriate experimental dietary model. Since previously reported experimental designs have been matter of concern, we developed a dietary model to compare the effect of t isomers and/or the saturation of FA independently of other variables. Wistar rats were fed diets containing identical amounts of nutrients and high levels of dietary fats (200 g/kg) for 30 d. Dietary fat rich in t-FA was compared with fat rich in saturated (s) FA or rich in cis (c) FA, maintaining the same length of C chain of the FA. The fats were obtained through isomerization or hydrogenation of the c-FA present in the control fat. Apparent fat absorption, energy efficiency and triacylglycerol levels in serum and liver were different in rats fed t-FA or s-FA than c-FA. The apparent fat absorption was (%): s-FA 85·7 (SD 3·4)<T-FA 93·1 (sd 0·4)<c-FA 96·7 (sd 1·1) (P<0·05). The efficiency of energy utilization was lower in t-FA (11·7 %) and s-FA (18·5 %) diets, reaching statistical significance only between s-FA and c-FA. A striking finding was the change in the lipid profile in serum and liver. Serum and hepatic triacylglycerol levels were greater for t-FA and s-FA diets than in c-FA; however, the increases on serum triacylglycerol concentrations were greater with the s-FA diet and the increases on hepatic triacylglycerol content were greater with t-FA. Knowledge of the t-FA effects on this kind of experimental dietary model could contribute to determine the potential risk of t-FA intake for man.

Keywords

Trans fatty acidsIsomerized fatHydrogenated fatDietary fat
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 5 , May 2003 , pp. 631 - 638
Copyright
Copyright © The Nutrition Society 2003

References

Allison, DB, Egan, K, Barraj, L, Caughman, C, Infante, M & Heimbach, JT (1999) Estimated intakes of trans fatty and other fatty acids in the US population. Journal of the American Dietetic Association 99 166–174.CrossRefGoogle ScholarPubMed
Aro, A, Van Amelsvoort, J, Becker, W, van Erp-Baart, MA, Kafatos, A, Leth, T & van Poppel, G (1998) Trans fatty acids in dietary fats and oils from 14 European countries: the transfair study. Journal of Food Composition and Analysis 11 137–149.CrossRefGoogle Scholar
Ascherio, A, Katan, MB, Zock, PL, Stampfer, MJ & Willet, WC (1999) Trans fatty acids and coronary heart disease. New England Journal of Medicine 340 1994–1998.CrossRefGoogle ScholarPubMed
Atal, S, Zarnowski, MJ, Cushman, SW & Sampugna, J (1994) Comparison of body weight and adipose tissue in Male C57BI/6J mice fed diets with and without trans fatty acids. Lipids 29 319–325.CrossRefGoogle Scholar
Bernal, CA, Martinelli, MI & Mocchiutti, NO (2002) The effect of dietary exposure of rats to di(2-ethyl hexyl) phthalate on their metabolic efficiency. Food Additives and Contaminants 19 1091–1096.CrossRefGoogle ScholarPubMed
Bernal, C, Gutman, R & Lombardo, Y (1995) The duration of feeding on a sucrose-rich diet determines variable in vitro effects of insulin and fructose on rat liver triglyceride metabolism. Journal of Nutritional Biochemistry 6 422–430.CrossRefGoogle Scholar
Bernal, C, Vazquez, J & Adibi, S (1992) Liver triglyceride concentration and body protein metabolism in ethanol-treated rats: Effect of energy and nutrient supplementation. Gastroenterology 103 289–295.CrossRefGoogle ScholarPubMed
Bernard, A, Echinard, B & Carlier, H (1987) Differential intestinal absorption of two fatty acid isomers: elaidic and oleic acids. American Journal of Physiology 253 G751–G759.Google ScholarPubMed
Canolty, NL & Koong, LJ (1976) Utilisation of energy for maintenance and for fat and lean gains by mice selected for rapid postweaning growth rate. Journal of Nutrition 106 1202–1208.CrossRefGoogle ScholarPubMed
Clevidence, BA, Judd, JT, Schaefer, EJ, Jenner, JL, Lichtenstein, AH, Muesing, RA, Wittes, J & Sunkin, ME (1997) Plasma lipoprotein(a) levels in men and women consuming diets enriched in saturated, cis-, or trans-monounsaturated fatty acids. Arteriosclerosis, Thrombosis and Vascular Biology 17 1657–1661.CrossRefGoogle ScholarPubMed
DeGroot, MH (1975) Probability and Statistics. Reading, MA: Addison-Wesley Publishing Company, Inc.Google Scholar
Emken, EA, Adlof, RO, Rodwedder, WK & Gulley, RM (1983) Incorporation of deuterium-label ed trans- and cis-13-octadecanoic acids in human plasma lipids. Journal of Lipid Research 24 34–46.CrossRefGoogle Scholar
Firestone, D (1999) Oils and fats. In Official Methods of Analysis, 16th ed., 5th revision, pp. 41.27A–41.27D [Cunniff, P, editor]. Gaithersburg, MD: AOAC International.Google Scholar
Jones, PJ (1989) Effect of fatty acid composition of dietary fat on energy balance and expenditure in hamsters. Canadian Journal of Physiological Pharmacology 67 994–998.CrossRefGoogle ScholarPubMed
Judd, JT, Clevidence, BA, Muesing, RA, Wittes, J, Sunkin, ME & Podczasy, JJ (1994) Dietary trans fatty acids: effects on plasma lipids and lipoproteins of healthy men and women. American Journal of Clinical Nutrition 59 861–868.CrossRefGoogle ScholarPubMed
Kalogeresis, TJ, Gray, L, Yeh, YY & Tso, P (1996) Triacylglycerol and cholesterol transport during absorption of glycerol trioleate vs. glycerol trielaidate. American Journal of Physiology 270 G268–G276.Google Scholar
Katan, MB, Zock, PL & Mensink, RP (1995) Trans fatty acids and their effects on lipoproteins in human. Annual Review of Nutrition 15 473–493.CrossRefGoogle Scholar
Kris-Etherton, PM ed. (1995), Trans fatty acids and coronary heart disease risk. Report of the Expert Panel on trans fatty acids and coronary heart disease. American Journal of Clinical Nutrition 62 655S–708S.Google Scholar
Lawson, LD & Holman, RT (1981) Beta-oxidation of the geometric and positional isomers of octadecenoic acid by rat heart and liver mitochondria. Biochimica et Biophysica Acta 665 60–65.CrossRefGoogle ScholarPubMed
Lawson, LD & Kummerow, FA (1978) Beta-oxidation of the coenzyme A esters of vaccenic, elaidic and petroselaidic acids by rat heart mitochondria. Lipids 14 501–503.CrossRefGoogle Scholar
Lawson, LD & Kummerow, FA (1979) Beta-oxidation of the coenzyme A esters of elaidic, oleic and stearic acids and their full-cycle intermediates by rat heart mitochondria. Biochimica et Biophysica Acta 573 245–254.CrossRefGoogle ScholarPubMed
Lichtenstein, AH, Ausman, LM, Carrasco, W, Jenner, JL, Ordovas, JM & Schaefer, EJ (1993) Hydrogenation impairs the hypolipidemic effect of corn oil in humans: hydrogenation, trans fatty acids, and plasma lipids. Arteriosclerosis and Thrombosis 13 154–161.CrossRefGoogle ScholarPubMed
Louheranta, AM, Turpeinen, AK, Vidgren, HM, Schwab, US & Uusitupa, MI (1999) A high-trans fatty acid diet and insulin sensitivity in young healthy women. Metabolism, Clinical and Experimental 48 870–875.CrossRefGoogle ScholarPubMed
Mensink, RP & Katan, MB (1990) Effect of dietary trans fatty acids on high-density and low-density lipoproteins cholesterol levels in healthy subjects. New England Journal of Medicine 323 439–445.CrossRefGoogle ScholarPubMed
Mocchiutti, N & Bernal, C (1997) Effects of chronic di (2-ethyl hexyl) phthalate intake on the secretion and removal rate of triglyceride-rich lipoproteins in rats. Food and Chemical Toxicology 35 1017–1021.CrossRefGoogle Scholar
Mohamedain, MM & Kummerow, FA (1999) Hydrogenated fat high in trans monoenes with an adequate level of linoleic acid has no effect on prostaglandin synthesis in rats. Journal of Nutrition 129 15–24.Google Scholar
Mutanen, M & Aro, A (1997) Coagulation and fibrinolysis in healthy subjects consuming high stearic or trans fatty acid diets. Thrombosis and Haemostasis 77 99–104.Google ScholarPubMed
Nicolosi, RJ, Wilson, TA, Rogers, EJ & Kritchevsky, D (1998) Effects of specific fatty acids (8:0, 14:0, cis-18:1, trans-18:1) on plasma lipoproteins, early atherogenic potential, and LDL oxidative properties in the hamster. Journal of Lipid Research 39 1972–1980.CrossRefGoogle Scholar
Phan, CT & Tso, P (2001) Intestinal lipid absorption and transport. Frontiers in Bioscience 6 d299–d319.CrossRefGoogle ScholarPubMed
Reeves, PG, Nielsen, FH & Fahey, GC (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. Journal of Nutrition 123 1939–1951.CrossRefGoogle Scholar
Roos, NM, Schouten, EG & Katan, MB (2001) Consumption of a solid fat rich in lauric acid results in a more favorable serum lipid profile in healthy men and women than consumption of a solid fat rich in trans-fatty acids. Journal of Nutrition 131 242–245.CrossRefGoogle Scholar
Ros, E (2000) Intestinal absorption of triglyceride and cholesterol. Dietary and pharmacological inhibition to reduce cardiovascular risk. Atherosclerosis 151 357–379.CrossRefGoogle ScholarPubMed
Sundram, K, Ismail, A, Hayes, KC, Jeyamalar, R & Pathmanathan, R (1997) Trans (elaidic) fatty acids adversely affect the lipoprotein profile relative to specific saturated fatty acids in humans. Journal of Nutrition 127 514S–529S.CrossRefGoogle ScholarPubMed
Takeuchi, H, Matsuo, T, Tokuyama, K, Shimomura, Y & Suzuki, M (1995) Diet-induced thermogenesis is lower in rats fed a lard diet than in those fed a high oleic acid safflower oil diet, a safflower oil diet or a linseed oil diet. Journal of Nutrition 125 920–925.Google ScholarPubMed
Thomson, ABR, Garg, M, Keelan, M & Doring, K (1994) Feeding trans fatty acids to rats has no effect on the intestinal uptake of glucose, fatty acids or cholesterol. Digestion 55 405–409.CrossRefGoogle ScholarPubMed
Windham, WR (1999) Animal feed. In Official Methods of Analysis, 16th ed., 5th revision, pp. 4·1–4·45 [Cunniff, P, editor]. Gaithersburg, MD: AOAC International.Google Scholar