Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-12-02T19:38:43.573Z Has data issue: false hasContentIssue false
  • English
  • Français

Mechanism underlying the inhibitory effect of high calcium carbonate intake on iron bioavailability from ferrous sulphate in anaemic rats

Published online by Cambridge University Press:  09 March 2007

K. J. H. Wienk ,
J. J. M. Marx ,
A. G. Lemmens ,
E. J. Brink ,
R. Van Der Meer  and
A. C. Beynen
K. J. H. Wienk
Affiliation:
Department of Laboratory Animal Science, Utrecht University, PO Box 80166, 3508 TD, Utrecht, The Netherlands
J. J. M. Marx
Affiliation:
Department of Internal Medicine, Utrecht University Academic Hospital, Utrecht, The Netherlands
A. G. Lemmens
Affiliation:
Department of Laboratory Animal Science, Utrecht University, PO Box 80166, 3508 TD, Utrecht, The Netherlands
E. J. Brink
Affiliation:
Unilever Research Laboratorium, Vlaardingen, The Netherlands
R. Van Der Meer
Affiliation:
Netherlands Institute for Dairy Research, Ede, The Netherlands
A. C. Beynen
Affiliation:
Department of Laboratory Animal Science, Utrecht University, PO Box 80166, 3508 TD, Utrecht, The Netherlands
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The influence of high CaCO3, intake on the bioavailability of Fe from FeSO4, was assessed during Fe repletion of rats with Fe-deficiency-induced anaemia. Fe-deficient rats with a mean blood haemoglobin concentration of 4. 1 mmol/l were fed on purified Fe-adequate diets containing either 6.2 or 25.0g CaCO,/kg (ten rats per group). Haemoglobin repletion after 14 d was significantly depressed by high CaCO, intake (9.5 v. 9.8 mmol/l for high and low CaCO, intake respectively; P = 0·03), as was apparent Fe retention (367 v. 552 µg/d during days 5–7, P<0·001; 146 v. 196 µg/d during days 19–21, P < 0·001). The concentration of Fe in the liquid phase of the proximal half of the small intestine was significantly lower in the high-CaCO, group (3.71 v. 520 µg/g digesta; P = 0-02). Mucosal uptake and mucosal transfer of Fe were determined with orally administered 59Fe and Cr as a non-absorbable marker. Mucosal transfer was significantly diminished by CaCO, loading (90 v. 100% of mucosal uptake; P = 0·04), whereas mucosal uptake was not. 59Fe retention values at 14 d after administration were not significantly different (57.6 v. 51.9%; P = 0·14). Fe contents of liver and spleen were significantly decreased by high compared with low CaCO, intake (879 v. 590 pg Fe in liver, P < 0·001; 92 v. 63pg Fe in spleen, P <0·001). It is concluded that high intake of CaCO, depresses Fe bioavailability in rats. The CaCO,-induced decrease in Fe solubility in the digesta probably was associated with an increased efficiency of mucosal Fe uptake so that the amount of mucosal uptake remained unaltered. The CaCO,-induced decrease in Fe transfer through the mucosal cytoplasm and/or basolateral membrane may have been responsible for the concurrent decrease in Fe bioavailability.

Keywords

Non-haem FeCalcium carbonate intake
Type
Effect of calcium carbonate on iron bioavailability
Information
British Journal of Nutrition , Volume 75 , Issue 1 , January 1996 , pp. 109 - 120
Copyright
Copyright © The Nutrition Society 1996

References

REFERENCES

Association of Official Analytical Chemists (1990). Oficial Methods of Analysis of the Association of Oficial Analytical Chemists, 15th ed. [Helrich, K. editor] Arlington: AOAC.Google Scholar
Barton, J. C., Conrad, M. E. & Parmley, R. T. (1983). Calcium inhibition of inorganic iron absorption in rats. Gastroenterology 84, 90–101.CrossRefGoogle ScholarPubMed
Bothwell, T. H., Charlton, R. W., Cook, J. D. & Finch, C. A. (1979). Iron absorption. In Iron Metabolism in Man, pp. 256–283. Oxford: Blackwell scientific Publications.Google Scholar
Brouwer, I. A., Lemmens, A. G. & Beynen, A. C. (1993). Dietary fructose v. glucose lowers ferrous-iron absorption in rats. British Journal of Nutrition 70, 171–178.CrossRefGoogle ScholarPubMed
Chapman, D. G. & Campbell, J. A. (1957). Effect of calcium and phosphorus salts on the utilization of iron by anaemic rats. British Journal of Nutrition 11, 127–133..CrossRefGoogle ScholarPubMed
Cook, J. D., Dassenko, S. A. & Whittaker, P. (1991). Calcium supplementation: effect on iron absorption. American Journal of Clinical Nutrition 53, 106–111.CrossRefGoogle ScholarPubMed
Dawson-Hughes, B., eligson, F. H. & Hughes, V. A. (1986). Effects of calcium carbonate and hydroxyapatite on zinc and iron retention in postmenopausal women. American Journal of Clinical Nutrition 44, 83–88.CrossRefGoogle ScholarPubMed
Donaldson, R. M. Jr & Barreras, R. F. (1966). Intestinal absorption of trace quantities of chromium. Journal of Laboratory and Clinical medicine 68, 484–493.Google ScholarPubMed
Freeman, S. & Ivy, A. C. (1942). The influence of antacids upon iron retention by the anemic rat. American Journal of Physiology 137, 706–709.CrossRefGoogle Scholar
Fritz, J. C., Pla, G. W., Harrison, B. N. & Clark, G. A. (1974). Collaborative study of the rat hemoglobin repletion test for bioavailability of iron. Journal of the Association of Oficial Analytical Chemists 51, 513–517.Google Scholar
Hallberg, L., Brune, M., Erlandsson, M., Sandberg, A. S. & Rossander-Hultin, L. (1991). Calcium: effect of different amounts on nonheme- and heme-iron absorption in humans. American Journal of Clinical Nutrition 53, 112–119.CrossRefGoogle ScholarPubMed
Hallberg, L., Rossander-Hulthtn, L., Brune, M. & Gleerup, A. (1992). Calcium and iron absorption: mechanism of action and nutritional importance. European Journal of Clinical Nutrition 46, 317–327.Google ScholarPubMed
Hallberg, L., Rossander-Hulthtn, L., Brune, M. & Gleerup, A. (1993). Inhibition of haem-iron absorption in man by calcium. British Journal of Nutrition 69, 533–540.CrossRefGoogle Scholar
Heth, D. A. & Hoekstra, W. G. (1965). Zinc-65 absorption and turnover in rats. I. A procedure to determine zinc-65 absorption and the antagonistic effect of calcium in a practical diet. Journal of Nutrition 85, 367–374.CrossRefGoogle Scholar
McCall, M. G., Newman, G. E., O'Brien, J. R. P. & Witts, L. J. (1962). Studies in iron metabolism. 2. The effects of experimental iron deficiency in the growing rat. British Journal of Nutrition 16, 305–323.CrossRefGoogle Scholar
Marx, J. J. M. (1979). Mucosal uptake, mucosal transfer and retention of iron, measured by whole-body counting. Scandinavian Journal of Haemutology 23, 293–302.CrossRefGoogle ScholarPubMed
Marx, J. J. M., Van den Beld, B., Van Dongen, R. & Strackee, L. H. (1980). Simultaneous measurement of 59Fe and 51Cr in iron absorption studies using a whole-body scanner with mobile shielding. Nuclear Medicine 19, 140–145.Google ScholarPubMed
Miller, D. D., Schricker, B. R., Rasmussen, R. R. & Van Campen, D. (1981). An in vitro method for estimation of iron availability from meals. American Journal of Clinical Nutrition 34, 2248–2256.CrossRefGoogle Scholar
National Research Council (1978). Nutrient requirements of domestic animals. Nutrient Requirements of Laboratory Animals no. 10, 3rd ed. Washington, DC: National Academy of Sciences.Google Scholar
Prather, T. A. & Miller, D. D. (1992). Calcium carbonate depresses iron bioavailability in rats more than calcium sulfate or sodium carbonate. Journal of Nutrition 122, 327–332.CrossRefGoogle ScholarPubMed
Sijtsma, K. W., Van den Berg, G. J., Lemmens, A. G., West, C. E. & Beynen, A. C. (1993). Iron status in rats fed on diets containing marginal amounts of vitamin A. British Journal of Nutrition 70, 777–785.CrossRefGoogle ScholarPubMed
Statistical Package for Social Sciences (1988). SPSS\PC+ V2.0 Base Manual. Chicago, Ill: SPSS Inc.Google Scholar
Van Dokkum, W. (1992). Significance of iron bioavailability for iron recommendations. Biological Trace Element Research 35, 1–11.CrossRefGoogle ScholarPubMed
Wheby, M. S. (1970). Site of iron absorption in man. Scandinavian Journal of Haemutology 7, 56–62.CrossRefGoogle ScholarPubMed
Yokoi, K., Kimura, M. & Itokawa, Y. (1991). Effect of dietary iron deficiency on mineral levels in tissues of rats. Biological Trace Element Research 29, 257–265.CrossRefGoogle ScholarPubMed