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Maternal protein reserves and their influence on lactational performance in rats 2. Effects of dietary protein restriction during gestation and lactation on tissue protein metabolism and Na+,K+-ATPase(EC 3.6.1.3) activity

Published online by Cambridge University Press:  09 March 2007

A. P. Pine ,
N. S. Jessop ,
G. F. Allan  and
J. D. Oldham
A. P. Pine
Affiliation:
Institute of Ecology and Resource Management, University of Edinburgh, West Mains Road, Edinburgh EH9 3JG
N. S. Jessop
Affiliation:
Institute of Ecology and Resource Management, University of Edinburgh, West Mains Road, Edinburgh EH9 3JG
G. F. Allan
Affiliation:
Institute of Ecology and Resource Management, University of Edinburgh, West Mains Road, Edinburgh EH9 3JG
J. D. Oldham
Affiliation:
Genetics and Behavioural Science Department, Scottish Agricultural College, West Mains Road, Edinburgh EH9 3JG
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Abstract

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Changes in tissue protein synthesis and an associated membrane transport system in rats were investigated during lactation and under conditions of dietary protein restriction. Following mating, female Sprague-Dawley rats (second parity) were caged individually and offered a high-protein diet (H; 215 g crude protein (N × 6.25; CP)/kg dry matter (DM)) ad lib. until day 12 of gestation. Subsequently half continued to receive diet H, whilst the remainder were offered a low-protein diet (L; 65 g CP/kg DM) until parturition. On day 1 of lactation females were then allocated to either diet H or another low-protein diet (L2; 90 g CP/kg DM) which were offered ad lib. until day 13 of lactation, giving four lactation groups HH, LH, HL2 and LL2. On days 1 and 13 of lactation groups of females were used in the estimation of tissue protein synthesis (flooding dose of [3H] phenylalanine) and Na+, K+-ATPase (EC 3.6.1.3) activity (polarographically) in skeletal muscle, mammary gland, liver and duodenal mucosa. By day 1 of lactation diet L had reduced fractional and absolute synthesis rates (FSR and ASR) of muscle protein (P < 0.05) and the O2 consumption associated with Na+,K+-ATPase, although not significantly (P < 0.10). Rates of protein synthesis in the other tissues studied were not affected on day 1 of lactation by the gestation dietary treatment. By day 13 of lactation the feeding of diet L2 had reduced muscle FSR and ASR of group HL2 to rates that were lower than those on day 1 (P < 0.05), comparable to those of group LL2 and lower than those of groups HH and LH (P < 0.05). Diet H had allowed group LH to increase their muscle protein synthesis compared with that on day 1 (P < 0.05). Muscle Na+,K+-ATPase activity on day 13 of lactation was also lower in groups offered diet L2 (P < 0.05). Mammary protein synthesis was increased during lactation with the feeding of diet H (P < 0.05), which was prevented by diet L2 such that rates of groups HL2 and LL2 were lower than those of the two high-protein groups on day 13 (P < 0.01). Mammary respiration and in particular Na+,K+-ATPase activity was increased during lactation by the feeding of diet H (P < 0.05). Rates of protein synthesis and respiration in liver and duodenal mucosa were not significantly affected by the gestational or lactational dietary treatments. Calculated rates of muscle protein degradation suggest that whilst the loss of muscle protein in group HL2 during lactation might have been promoted by the decline in synthesis, the increase in degradation may have been quantitatively more important.

Keywords

LactationProtein synthesisNa+,K+-ATPaseRat
Type
Effects of dietary restrictions during gestation and lactation
Information
British Journal of Nutrition , Volume 72 , Issue 2 , August 1994 , pp. 181 - 197
Copyright
Copyright © The Nutrition Society 1994

References

REFERENCES

Adeola, O., Young, L. G., McBride, B. W. & Ball, R. 0. (1989). In vitro Na+, K+ -ATPase (EC 3.6.1.3)-dependent respiration and protein synThesis in skeletal muscle of pigs fed at three dietary protein levels. British Journal of Nutrition 61, 453465.CrossRefGoogle Scholar
Albers, R. W., Kovala, G. J. & Siegel, C. J. (1968). Studies on the interaction of ouabain and other active steroids with sodium potassium activated adenosine triphosphatase. Molecular Pharmacology 4, 326–336.Google ScholarPubMed
Ashford, A. J. & Pain, V. M. (1986). Effect of diabetes on the rates of synThesis and degradation of ribosomes in rat muscle and liver in vivo. Journal of Biological Chemistry 261, 4059–4065.CrossRefGoogle ScholarPubMed
Baracos, V. E., Brun-Bellut, J. & Marie, M. (1991). Tissue protein synThesis in lactating and dry goats. British Journal of Nutrition 66, 451–465.CrossRefGoogle ScholarPubMed
Bartley, J. C. & Abraham, S. (1976). The absolute rate of fatty acid synThesis by mammary gland slices from lactating rats. Journal of Lipid Research 17, 467–477.CrossRefGoogle ScholarPubMed
Bryant, D. T. W. & Smith, R. W. (1982). The effect of lactation on protein synThesis in ovine skeletal muscle. Journal of Agricultural Science, Cambridge 99, 319–323.CrossRefGoogle Scholar
Canas, R., Romero, J. J. & Baldwin, R. L. (1982).Maintenance energy requirements during lactation in rats. Journal of Nutrition 112, 1876–1880.CrossRefGoogle ScholarPubMed
Friggens, N. C., Hay, D. E. F. & Oldham, J. D. (1993). Interactions between major nutrients in the diet and the lactational performance of rats. British Journal of Nutrition 69, 59–71.CrossRefGoogle ScholarPubMed
Garlick, P. J., Fern, M. & Preedy, V. R. (1983). The effect of insulin infusion and food intake on muscle protein synThesis in post absorptive rats. Biochemical Journal 210, 669–676.CrossRefGoogle Scholar
Garlick, P. J., McNurlan, M. A. & Preedy, V. R. (1980). A rapid and convenient technique for measuring the rate of protein synThesis in tissues by the injection of [3HI] phenylalanine. Biochemical Journal 192, 719–723.CrossRefGoogle ScholarPubMed
Gregg, V. A. & Milligan, L. P. (1982 a). Role of Na+,K+-ATPase in muscular energy expenditure of warm- and cold-exposed sheep. Canadian Journal of Animal Science 62, 123–132.CrossRefGoogle Scholar
Gregg, V. A. & Milligan, L. P. (1982 b). In vitro energy costs of Na+,K+-ATPase activity and protein synthesis from calves differing in age and breed. British Journal of Nutrition 48, 65–71.CrossRefGoogle ScholarPubMed
Gregg, V. A. & Milligan, L. P. (1982 c). 0, consumption and Na+,K+-ATPase dependent respiration in muscle of lambs and lactating and non lactating ewes. In Energy Metabolism of Farm Animals, pp. 66–69 [Ekern, A. and Sundstol, F., editors]. Aas, Norway: Agricultural University of Norway.Google Scholar
Jansen, G. R. & Hunsaker, H. (1986). Effect of dietary protein and energy on protein synThesis during lactation in rats. Journal of Nutrition 116, 957–968.CrossRefGoogle ScholarPubMed
Jessop, N. S. (1988). Estimation of energy expenditure associated with Na+,K+-ATPase activity in ovine liver. Proceedings of the Nutrition Society 47, 118A.Google Scholar
Lichtenberger, L. M. & Trier, J. S. (1979). Changes in gastrin levels, food intake and duodenal mucosal growth during lactation. American Journal of Physiology 237, E98–EIO5.Google ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
McBride, B. W. & Milligan, L. P. (1984). The effect of lactation on ouabain sensitive respiration of the duodenal mucosa of cows. Canadian Journal of Animal Science 64, 817–824.CrossRefGoogle Scholar
McBride, B. W. & Milligan, L. P. (1985 a). Magnitude of ouabain-sensitive respiration in the liver of growing and lactating sheep. British Journal of Nutrition 54, 293–303.CrossRefGoogle Scholar
McBride, B. W. & Milligan, L. P. (1985 b). Influence of food intake and starvation on the magnitude of Na+,K+-ATPase (EC 3.6.1.3)-dependent respiration in duodenal mucosa of sheep. British Journal of Nutrition 53, 605–614.CrossRefGoogle Scholar
McNurlan, M. A., Tomkins, A. M. & Garlick, P. J. (1979). The effect of starvation on the rate of protein synThesis in the rat liver and small intestine. Biochemical Journal 178, 373–379.CrossRefGoogle ScholarPubMed
Mahan, D. C. & Mangan, L. T. (1975). Evaluation of various protein sequences on the nutritional carry over from gestation to lactation with first litter sows. Journal of Nutrition 105, 1291–1298.CrossRefGoogle ScholarPubMed
Mayel-Afshar, S. & Gimble, R. F. (1983). Changes in protein turnover during gestation in the foetus, placenta, liver, muscle and whole body of rats given a low protein diet. Biochimica et Biophysica Acta 756, 182–190.CrossRefGoogle ScholarPubMed
Millican, P. E., Vernon, R. G. & Pain, V. M. (1987). Protein metabolism in the mouse during pregnancy and lactation. Biochemical Journal 248, 251–257.CrossRefGoogle ScholarPubMed
Milligan, L. P. & Summers, M. (1986). The biological basis of maintenance and its relevance to assessing responses to nutrients. Proceedings of the Nutrition Society 45, 185–193.CrossRefGoogle ScholarPubMed
Munro, H. N. & Fleck, A. (1969). Analysis of tissues and body fluids for nitrogenous constituents. In Mammalian Protein Metubolism, vol. 3, pp 425–465 [Munro, H. N., editor]. New York: Academic Press.Google Scholar
National Research Council (1978). Nutrient Requirements of Laboratory Animals, 3rd ed. Washington, DC: National Academy of Sciences.Google Scholar
Pine, A. P., Jessop, N. S. & Oldham, J. D. (1994). Maternal protein reserves and their influence on lactational performance in rats. British Journal of Nutrition 71, 13–27.CrossRefGoogle ScholarPubMed
Rosso, P., Keyou, G., Bassi, J. A. & Slusser, W. M. (1981). Effect of malnutrition during pregnancy on the development of the mammary glands of rats. Journal of Nutrition 111, 1937–1941.CrossRefGoogle ScholarPubMed
Sainz, R. D., Calvert, C. C. & Baldwin, R. L. (1986). Relationships among dietary protein, food intake and tissue protein turnover in lactating rats. Journal of Nutrition 116, 1820–1829.CrossRefGoogle ScholarPubMed
Sampson, D. A., Hunsaker, H. A. & Jansen, G. R. (1986). Dietary protein quality, protein quantity and food intake: effects on lactation and on protein synthesis and issue composition in mammary tissue and liver of rats. Journal of Nutrition 116, 365–375.CrossRefGoogle Scholar
Sampson, D. A. & Jansen, G. R. (1984 a). Protein and energy nutrition during lactation. Annual Review of Nutrition 4, 43–47.CrossRefGoogle ScholarPubMed
Sampson, D. A. & Jansen, G. R. (1984 b). Protein synthesis during lactation: no circadian variation in mammary gland and liver of rats fed diets varying in protein quality and level of intake. Journal of Nutrition 114, 1470–1478.CrossRefGoogle ScholarPubMed
Siebrits, F., Martinez, J. A. & Buttery, P. J. (1985). The effect of lactation on the fractional synthetic rate of protein in the liver and muscle of rats. International Journal of Biochemistry 17, 731–732.CrossRefGoogle ScholarPubMed
Swick, R. W. & Benevenga, N. J. (1977). Labile protein reserves and protein turnover. Journal of Dairy Science 60, 505–515.CrossRefGoogle ScholarPubMed
Vandenburgh, H. H. & Kaufman, S. (1981). Stretch induced growth of skeletal myotubes correlates with activation of the sodium pump. Journal of Cellular Physiology 109,20%214.CrossRefGoogle ScholarPubMed
Vincent, R. & Lindsay, D. B. (1985). Effect of pregnancy and lactation on muscle protein metabolism in sheep. Proceedings of the Nutrition Society 44, 77A.Google Scholar
Waterlow, J. C., Garlick, P. J. & Millward, D. J. (1978). In Protein Turnover in Mammalian Tissues and in the Whole Body, pp. 627–631. New York: North-Holland.Google Scholar
Williamson, D. H. (1980). Integration of metabolism in tissues of the lactating rat. FEES Letters, 117(Suppl. I), K93-K105.CrossRefGoogle ScholarPubMed
Williamson, D. H., Munday, M. R. & Jones, R. G. (1984). Biochemical basis of dietary influences on the synThesis of the macro nutrients of rat milk. Federation Proceedings 43, 2443–2447.Google Scholar