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Maternal manipulation of brown adipose tissue and liver development in the ovine fetus during late gestation

Published online by Cambridge University Press:  09 March 2007

Lynne Clarke ,
Michael J. Bryant ,
Michael A. Lomax  and
Michael E. Symonds
Lynne Clarke
Affiliation:
School of Animal and Microbial Sciences, University of Reading, Whiteknights, PO Box 228, Reading RG6 2AJ
Michael J. Bryant
Affiliation:
Department of Agriculture, University of Reading, Whiteknights, PO Box 228, Reading RG6 2AJ
Michael A. Lomax
Affiliation:
School of Animal and Microbial Sciences, University of Reading, Whiteknights, PO Box 228, Reading RG6 2AJ
Michael E. Symonds
Affiliation:
School of Animal and Microbial Sciences, University of Reading, Whiteknights, PO Box 228, Reading RG6 2AJ
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Abstract

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We examined the effect of maternal chronic cold exposure, induced by winter-shearing ewes 4 weeks before their predicted lambing date, on brown adipose tissue (BAT) and liver development in lambs. Fetuses were sampled from under-fed (60% of energy requirements for maintenance and pregnancy of an unshorn ewe) shorn or unshorn ewes at 126,140 and 145 d of gestation. Lambs were sampled from ewes within 2 h of birth. Throughout gestation fetal body, BAT and liver weights were similar in shorn and unshorn groups. The level of GDP binding to mitochondrial uncoupling protein remained low throughout gestation, but increased dramatically after birth. Lambs born to shorn ewes possesd more mitochondrial protein and exhibited a significantly higher total thermogenic activity in BAT. Type I iodothyronine 5 deiodinas(EC 3.8.1.4) activity in BAT peaked at birth, as did hepatic iodothyronine Sdeiodinase activity and was significantly greater in lambs born to under-fed shorn ewes, which exhibited a higher plasma triiodothyronine concentration. Chronic maternal adaptations to prolonged cold exposure appear to enable pregnant ewes to compensate for the negative effects of under-feeding on fetal growth and development

Keywords

Brown adipose tissueFetusLiver
Type
Animal Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 6 , June 1997 , pp. 871 - 883
Copyright
Copyright © The Nutrition Society 1997

References

Agricultural Research Council (1980). Requirements for energy. In The Nutritional Requirements of Ruminant Livestock, pp. 115–119. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Alexander, G. (1978). Quantitative development of adipose tissue in fetal sheep. Australian Journal of Biological Sciences 31, 489–503.CrossRefGoogle Scholar
Bell, A. W., Kennaugh, J. M., Battaglia, F. L., Makonski, E. L. & Meschia, G. (1986). Metabolic and circulatory studies of fetal lamb at mid gestation. American Journul of Physiology 250, E538–E544.Google Scholar
Casteilla, L., Champigny, O., Bouilland, F., Robelin, J. & Ricquier, D. (1989). Sequential changes in the expression of mitochondrial protein mRNA during the development of brown adipose tissue in bovine and ovine species. Biochemical Journal 257, 665–671.CrossRefGoogle ScholarPubMed
Casteilla, L., Forest, C., Robelin, J., Ricquier, D., Lombert, A. & Ailand, G. (1987). Characterisation of mitochondtial-uncoupling protein in bovine fetus and newborn calf. American Journal of Physiology 254, E627–E636.Google Scholar
Clarke, L., Andrews, D. C., Lomax, M. A. & Symonds, M. E. (1996). Effect of maternal glucose infusion on brown adipose tissue and liver development in the neonatal lamb. Reproduction, Fertility and Development 8, 1045–1054.CrossRefGoogle ScholarPubMed
Clarke, L., Darby, C. J., Lomax, M. A. & Symonds, M. E. (1994). Effect of ambient temperature during 1st day of life on thermoregulation in lambs delivered by cesarean section. Journul of Applied Physiology 76, 1481–1488.CrossRefGoogle ScholarPubMed
Desautels, M. & Himms-Hagen, J. (1978). Roles of noradrenaline and protein synthesis in the cold-induced increase in purine nucleotide binding by rat brown adipose tissue mitochondria. Canadian Journal of Biochemistry 57, 968–976.CrossRefGoogle Scholar
Gemmell, R. T. & Alexanaer, G. (1978). Ultrastructural development of adipose tissue in fetal sheep. Australian Journal of Biological Sciences 31, 505–515.CrossRefGoogle Scholar
Giralt, M., Casteilla, L., Vinas, O., Mampel, T., Iglesias, R., Robelin, J. & Villarroya, F. (1989). Iodothyronine 5′ deiodinase activity as an early event of prenatal brown fat differentiation in bovine development. Biochemical Journal 259, 555–559.CrossRefGoogle ScholarPubMed
Giralt, M., Martin, I., Iglesias, R., Vinas, O., Villarroya, F. & Mampel, T. (1990). Ontogeny and perinatal modulation of gene expression in rat brown adipose tissue: unaltered iodothyronine 5′-deiodinase activity is necessary for the response to environmental temperature at birth. European Journal of Biochemistry 193, 297–302.CrossRefGoogle ScholarPubMed
Hay, W. W. Jr., Sparks, J. W., Wilkening, R. B., Battaglia, F. C. & Meschia, G. (1984). Fetal glucose uptake and utilization as functions of maternal glucose concentrations. American Journul of Physiology 246, E237–E242.Google Scholar
Higham, F. C., Pillay, D. & Bailey, E. (1984). The effect of maternal diet on maternal and fetal hepatic and brown adipose tissue metabolites. Journal of Developmental Physiology 6, 153–158.Google Scholar
Himms-Hagen, J. (1989). Brown adipose tissue thermogenesis and obesity. Progress in Lipid Research 28, 67–115.CrossRefGoogle ScholarPubMed
Hyvarien, H., Pasenen, S., Heikura, H., Heinineva, R. & Laru, H. (1976). Effects of a cold environment on energy-related enzyme activities in the postnatal rat. Growth 40, 41–52.Google Scholar
Keppler, D. & Decker, K. (1984). Glycogen: metabolites and carbohydrates. In Methods of Enzymatic Analysis, 3rd ed., vol. 4, pp. 11–18 [Bergmeyer, J. and Grassl, M., editors]. Weinheim: Verlag Chemie.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Mellor, D. J. (1983). Nutritional and placental determination of foetal growth and consequences for the newborn lamb. British Veterinary Journal 139, 307–328.CrossRefGoogle ScholarPubMed
Mellor, D. J. & Cockburn, F. (1986). A comparison of energy metabolism in the new-born infant, piglet and lamb. Quarterly Journal of Experimental Physiology 71, 361–379.CrossRefGoogle ScholarPubMed
Molina, R. D., Meschia, G., Battaglia, F. L., Makonski, E. L. & Hay, W. W. (1991). Gestational maturation of placental glucose transfer capacity in sheep. American Journal of Physiology 261, R697–R704.Google ScholarPubMed
Niijima, A. (1986). Effect of glucose and other hexoses on efferent discharges of brown adipose tissue nerves. American Journal of Physiology 251, R240–R242.Google ScholarPubMed
Russel, A. J. F. (1984). Means of assessing the adequacy of nutrition of pregnant ewes. Livestock Production Science 11, 429–436.CrossRefGoogle Scholar
Slotkin, T. A. & Seidler, F. J. (1988). Adrenomedullary catecholamine release in the fetus and newborn: secretory mechanisms and their role in stress and survival. Journal of Developmental Physiology 10, 1–16.Google ScholarPubMed
Symonds, M. E. (1995). Pregnancy, parturition and neonatal development - interactions between nutrition and thyroid hormones. Proceedings of the Nutrition Sociery 54, 283–299.Google ScholarPubMed
Symonds, M. E., Bird, J. A., Clarke, L., Gate, J. J. & Lomax, M. A. (1995). Nutrition, temperature and homeostasis during perinatal development. Experimental Physiology 80, 907–940.CrossRefGoogle ScholarPubMed
Symonds, M. E., Bryant, M. J., Clarke, L., Darby, C. J. & Lomax, M. A. (1992). Effect of maternal cold exposure on brown adipose tissue and thermogenesis in the neonatal lamb. Journal of Physiology, London 455, 487–502.CrossRefGoogle ScholarPubMed
Symonds, M. E., Bryant, M. J. & Lomax, M. A. (1986). The effect of shearing on the energy metabolism of the pregnant ewe. British Journal of Nutrition 56, 635–643.CrossRefGoogle ScholarPubMed
Symonds, M. E., Bryant, M. J. & Lomax, M. A. (1988 a). Metabolic adaptation during pregnancy in winter-shorn sheep. Journal of Agricultural Science, Cambridge 111, 137–145.CrossRefGoogle Scholar
Symonds, M. E., Bryant, M. J., Shepherd, D. A. L. & Lomax, M. A. (1988)b. Glucose metabolism in shorn and unshorn pregnant sheep. British Journal of Nutrition 60, 249–263.CrossRefGoogle ScholarPubMed
Thompson, G. E., Bassett, J. M., Samson, D. E. & Slee, J. (1982). The effect of cold exposure of pregnant sheep on foetal plasma nutrients, hormones and birth weight. British Journal of Nutrition 48, 59–64.CrossRefGoogle ScholarPubMed
Wharton, D. C. & Tzagaloff, A. (1967). Cytochrome oxidase from beef heart mitochondria. Methods in Enzymology 10, 245–250.CrossRefGoogle Scholar
Wu, S. Y., Merryfield, M. L., Polk, D. H. & Fisher, D. A. (1990). Two pathways for thyroxine 5′-monodeiodinase in brown adipose tissue in fetal sheep: ontogenesis and divergent responses to hypothyroidism and 3,5,3′-triiodothyronine. Endocrinology 126, 1950–1958.CrossRefGoogle Scholar