Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-25T13:17:09.800Z Has data issue: false hasContentIssue false
  • English
  • Français

Long-term supplementation with selenate and selenomethionine: Selenium and glutathione peroxidase (EC 1.11.1.9) in blood components of New Zealand women

Published online by Cambridge University Press:  09 March 2007

Christine D. Thomson ,
Marion F. Robinson ,
Judy A. Butler  and
Phllip D. Whanger
Christine D. Thomson
Affiliation:
Department of Human Nutrition, University of Otago, PO Box 56, Dunedin, New Zealand
Marion F. Robinson
Affiliation:
Department of Human Nutrition, University of Otago, PO Box 56, Dunedin, New Zealand
Judy A. Butler
Affiliation:
Department Of Agricultural Chemistry, Oregon State University, Corvallis, Oregon 973314302, USA
Phllip D. Whanger
Affiliation:
Department Of Agricultural Chemistry, Oregon State University, Corvallis, Oregon 973314302, USA
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Thirty-three New Zealand women aged 18–23 years received daily for 32 weeks, 200 μg Se as Seenriched yeast (selenomethionine), or brewer's yeast mixed with selenate, or no added Se (placebo) in a double-blind trial. Se supplementation raised (P = 0.001), platelet glutathione peroxidase (EC 1.11.1.9; GSHPx) activity, and also Se and GSHPx in whole blood, erythrocytes and plasma. Selenomethionine was more effective in raising blood Se concentrations than selenate, but both were equally effective in raising GSHPx activities in whole blood, erythrocytes and plasma, indicating a similar bioavailability for the two forms. These observations and those of gel filtration studies of erythrocytes and plasma proteins reported elsewhere (Butler et al. 1991) are consistent with the incorporation of Se from selenomethionine into a general tissue protein pool while selenate is directly available for GSHPx synthesis, and explain the poorer correlation between Se and GSHPx in individuals with higher Se status. However, selenate raised platelet GSHPx activities to a greater extent than did selenomethionine suggesting some other effect of selenate on platelets which needs further investigation. A response of GSHPx activity in these New Zealand subjects indicates that their dietary Se intake is insufficient to meet recommended intakes based on the criterion of saturation of GSHPx activity, and could reflect a marginal Se status. The level of blood Se necessary for saturation of GSHPx of about 100 ng Se/ml whole blood confirms observations in earlier studies.

Keywords

SeleniumGlutathione peroxidasePlateletsWomen
Type
Effects of Selenium Supplementation
Information
British Journal of Nutrition , Volume 69 , Issue 2 , March 1993 , pp. 577 - 588
Copyright
Copyright © The Nutrition Society 1993

References

REFERENCES

Alfthan, G., Aro, A., Arvilommi, H. & Huttunen, J. K. (1991). Selenium metabolism and platelet glutathione peroxidase activity in healthy Finnish men: effect of selenium yeast, selenite and selenate. American Journal of Clinical Nutrition 53, 120 125.CrossRefGoogle ScholarPubMed
Avissar, N., Whitin, J. C., Allen, P. Z., Palmer, L. S. & Cohen, H. J. (1989). Antihuman plasma glutathione peroxidase antibodies: immunologic investigations to determine plasma glutathione peroxidase protein and selenium content in plasma. Blood 73, 318–323.CrossRefGoogle ScholarPubMed
Beaglehole, R., Jackson, R., Watkinson, J., Scragg, R. & Yee, R. L. (1990). Decreased blood selenium and risk of myocardial infarction. International Journal of Epidemiology 19, 918–922.CrossRefGoogle ScholarPubMed
Behne, D., Kyriakopoulos, A., Scheid, S. & Gessner, H. (1991). Effects of chemical form and dosage on the incorporation of selenium into tissue proteins in rats. Journal of Nutrition 121, 806–814.CrossRefGoogle ScholarPubMed
Behne, D. & Wolters, W. (1979). Selenium and glutathione peroxidase activity in the plasma and erythrocytes of non-pregnant and pregnant women. Journal of Clinical Chemistry and Clinical Biochemistry 17, 133–135.Google ScholarPubMed
Beilstein, M. A. & Whanger, P. D. (1983). Distribution of selenium and glutathione peroxidase in blood fractions from humans, rhesus and squirrel monkeys, rats and sheep. Journal of Nutrition 113, 2138–2146.CrossRefGoogle ScholarPubMed
Beilstein, M. A. & Whanger, P. D. (1986). Deposition of dietary organic and inorganic selenium in rat erythrocyte proteins. Journal of Nutrition 116, 1701–1710.CrossRefGoogle ScholarPubMed
Brown, M. W. & Watkinson, J. H. (1977). An automated fluorimetric method for the determination of nanogram quantities of selenium. Analytica Chimica Acra 89, 29–35.CrossRefGoogle Scholar
Burk, R. F. (1986). Selenium and cancer: meaning of serum selenium levels. Journal Of Nutrition 116, 1584–1586.CrossRefGoogle ScholarPubMed
Butler, J. A., Thomson, C. D., Whanger, P. D. & Robinson, M. F. (1991). Selenium distribution in blood fractions of New Zealand women taking organic or inorganic selenium. American Journal of Clinical Nutrition 53, 748–754.CrossRefGoogle ScholarPubMed
Deagan, J. T., Beilstein, M. A. & Whanger, P. D. (1991). Chemical form of selenium in selenium containing proteins from human plasma. Journal of Inorganic Biochemistry 41, 261–268.CrossRefGoogle Scholar
Gornall, A. G., Bardawill, C. J. & David, M. M. (1949). Determination of serum protein by means of the Biuret reaction. Journal of Biological Chemistry 177, 751–766.CrossRefGoogle ScholarPubMed
Keshan Disease Research Group of the Chinese Academy of Medical Sciences (1979). Observations on effect of sodium selenite in prevention of Keshan disease. Chinese Medical Journal 92, 471–476.Google Scholar
Koivistoinen, P. & Huttunen, J. K. (1986). Selenium in food and nutrition in Finland. An overview on research and action. Annul of Clinical Research 18, 13–17.Google ScholarPubMed
Levander, O. A., Alfthan, G., Arvilommi, H., Gref, C. G., Huttunen, J. K., Kataja, M., Koivistoinen, P. & Pikkarainen, J. (1983). Bioavailability of selenium to Finnish men as assessed by platelet glutathione peroxidase activity and other blood parameters. American Journal of Clinical Nutrition 31, 887–897.CrossRefGoogle Scholar
Levander, O. A. & Burk, R. F. (1990). Selenium. In Present Knowledge of Nutrition, 6th ed., pp. 268–273 [Brown, M. L., editor]. Washington DC: ILSI, Nutrition Foundation.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the folic phenol reagent. Journal qf Biological Chemistry 193, 265–275.CrossRefGoogle Scholar
Neve, J., Vertongen, F. & Capel, P. (1988). Selenium supplementation in healthy Belgian adults: response in platelet glutathione peroxidase activity and other blood indices. American Journal of Clinical Nutrition 48, 139–143.CrossRefGoogle ScholarPubMed
Paglia, D. C. & Valentine, W. N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journnl of Laboratory and Clinical Medicine 70, 158–169.Google ScholarPubMed
Rea, H. M., Thomson, C. D., Campbell, D. R. & Robinson, M. F. (1979). Relation between erythrocyte selenium concentrations and glutathione peroxidase (EC 1. 11. 1.9) activities of New Zealand residents and visitors to New Zealand. Brirish Journal of Nutrition 42, 201–208.CrossRefGoogle ScholarPubMed
Robinson, J. R., Robinson, M. F., Levander, O. A. & Thomson, C. D. (1985). Urinary excretion of selenium by New Zealand and North American subjects on differing intakes. American Journal of Clinical Nutrition 41, 1023–1031.CrossRefGoogle ScholarPubMed
Robinson, M. F., Jenkinson, C. D., Gu, L., Thomson, C. D. & Whanger, P. D. (1989). Urinary excretion of selenium (Se) and trimethylselenonium (TMSe) by New Zealand women during long-term supplementation with selenate or selenomethionine (Semet). In Selenium in Biology and Medicine, pp, 250–253 [Wendel, A., editor]. Heidelberg: Springer-Verlag.CrossRefGoogle Scholar
Robinson, M. F. & Thomson, C. D. (1987). Status of the food supply and residents of New Zealand. In Selenium in Biology and Medicine, Part B, pp. 631–644 [Combs, G. F., Spallholz, J. E., Levander, O. A. and Oldfield, J. E., editors]. New York: AVI.Google Scholar
Stead, N. W., Leonard, S. & Carrol, R. (1985). Effect of selenium supplementation on selenium balance in the dependent elderly. American Journal of Medical Science 290, 228 233.CrossRefGoogle ScholarPubMed
Stephen, R. C., Saville, D. J. & Watkinson, J. H. (1989). The effects of sodium selenate applications on growth and selenium concentration in wheat. New Zealand Journal of Crop and Horticulturul Science. 17, 229–237.CrossRefGoogle Scholar
Takahashi, K., Avissar, N., Whitin, J. & Cohen, H. (1987). Purification aiid characterization of human plasma glutathione peroxidase: A selenoglycoprotein distinct from the known cellular enzyme. Archives qf Biochemistry and Biophysics 256, 677–686.CrossRefGoogle Scholar
Tarp, U., Hansen, J. C., Overvad, K., Thorling, E. B., Tarp, B. D. & Graudal, H. (1987). Glutathione peroxidase activity in patients with rheumatoid arthritis and in normal subjects: effects of long-term selenium supplementation. Arthritis and Rheumatism 30, 1162–1 166.CrossRefGoogle ScholarPubMed
Thomson, C. D. (1985). Selenium-dependent and non-selenium-dependent glutathione peroxidase in human tissues of New Zealand residents. Biochemistry International 10, 673 679.Google ScholarPubMed
Thomson, C. D. (1991). Clinical consequences and assessment of low selenium status. New Zealand Medical Journal 104, 376–377.Google ScholarPubMed
Thomson, C. D., Burton, C. E. & Robinson, M. F. (1978). On supplementing the selenium intake of New Zealanders. Short experiments with large doses of selenite and selenomethionine. British Journal of Nutrition 39, 579–587.CrossRefGoogle Scholar
Thomson, C. D., Ong, L. K. & Robinson, M. F. (1985). Effects of supplementation with high-selenium wheat bread on selenium, glutathione peroxidase and related enzymes in blood components of New Zealand residents. American Journal of Clinical Nutrition 41, 1015–1022.CrossRefGoogle ScholarPubMed
Thomson, C. D., Rea, H. M., Doesburg, V. M. & Robinson, M. F. (1977). Selenium concentration and glutathione peroxidase activities in whole blood of New Zealand residents. British Journal of Nutrition 37, 457–460.CrossRefGoogle ScholarPubMed
Thomson, C. D. & Robinson, M. F. (1986). Urinary and fecal excretions and absorption of a large supplement of selenium: superiority of selenate over selenite. American Journal of Clinical Nutririon 44, 659–663.CrossRefGoogle ScholarPubMed
Thomson, C. D. & Robinson, M. F. (1988). Food concentrations and dietary intakes of selenium in Otago, New Zealand. Proceedings of the New Zealand Truce Elements Group Conference, pp. 113–117. Canterbury: Lincoln College.Google Scholar
Thomson, C. D., Robinson, M. F., Campbell, D. R. & Rea, H. M. (1982). Effect of prolonged supplementation with daily supplements of selenomethionine and sodium selenite on glutathione peroxidase activity in blood of New Zealand residents. American Journal of Clinical Nutrition 36, 24–31.CrossRefGoogle ScholarPubMed
Thomson, C. D., Robinson, M. F. & Whanger, P. D. (1989). Selenium (Se) and glutathione peroxidase (GSHPx) in blood components of New Zealand women during long term supplementation with selenate or selenomethionine (Semet). In Selenium in Biology and Medicine, pp. 254–257 [Wendel, A., editor]. Heidelberg: Springer-Verlag.CrossRefGoogle Scholar
Thomson, C. D., Steven, S. M., van Rij, A. M., Wade, C. R. & Robinson, M. F. (1988). Selenium and vitamin E supplementation: activities of glutathione peroxidase in human tissues. American Journal of Clinical Nutrition 48, 316–323.CrossRefGoogle ScholarPubMed
van der Torre, H. W., Van Dokkum, W., Schaafsma, G., Wedel, M. & Ockhuizen, T. (1991). Effect of various levels of selenium in wheat and meat on blood Se status indices aiid on Se balance in Dutch men. Brirish Journal of Nutrition 65, 69–80.CrossRefGoogle Scholar
van Rij, A. M., Thomson, C. D., McKenzie, J. M. & Robinson, M. F. (1979). Selenium deficiency in total parenteral nutrition. American Journal of Clinical Nutrition 32, 2076–2085.CrossRefGoogle ScholarPubMed
Whanger, P. D., Beilstein, M. A., Thomson, C. D., Robinson, M. F. & Howe, M. (1988). Blood selenium and glutathione peroxidase activity of populations in New Zealand, Oregon and South Dakota. FASEB Journul 2, 2996–3002.CrossRefGoogle ScholarPubMed
Winer, B. J. (1971). Statistical Principles in Experimental Design, 2nd ed. New York, N.Y.: McGraw-Hill.Google Scholar