Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-27T13:38:00.096Z Has data issue: false hasContentIssue false
  • English
  • Français

Lipid composition and metabolism in liver and brain of vitamin B12-deficient rat sucklings

Published online by Cambridge University Press:  08 December 2008

B. Åkesson ,
C. Fehling  and
Margaretha Jägerstad
B. Åkesson
Affiliation:
Department of Physiological Chemistry, Department of Neurology, and Dalby Community Health Research Centre, University of Lund, Lund, Sweden
C. Fehling
Affiliation:
Department of Physiological Chemistry, Department of Neurology, and Dalby Community Health Research Centre, University of Lund, Lund, Sweden
Margaretha Jägerstad
Affiliation:
Department of Physiological Chemistry, Department of Neurology, and Dalby Community Health Research Centre, University of Lund, Lund, Sweden
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Rat sucklings (18-d-old) bred from vitamin B12-deprived dams were compared with vitamin B12-suplemented dams’offspring, which were considered normal rat sucklings.

2. The vitamin B12-deficient rat sucklings had lower body-weight, liver weight and brain weight.

3. Vitamin B12 deficiency was also evident from the tenfold lower concentrations of vitamin B12 in liver and cerebellum.

4. The concentration of liver lipid was markedly increased in vitamin B12-deficient rats; triacylglycerol accounted for most of the increase. In brain the lipid concentration was slightly decreased (P < 0.05).

5. The methylation of ethanolamine phosphoglyceride to choline phosphoglyceride was reduced in both liver and brain in vitamin B12-deficient rats, as measured after the administration of [14C]ethanolamine. A slight decrease in choline phosphoglyceride concentration could be a consequence of this finding. The composition of phospholipids was otherwise normal.

6. Odd-chain fatty acids (pentadecanoate and heptadecanoate) accumulated in both liver and brain of the vitamin B12-deficient rat sucklings and constituted approximately 1% of total fatty acid.

7. The biosynthesis of fatty acid and cholesterol from intraperitoneally-injected 3H2O and [14C]propionate was unchanged in vitamin B12 deficiency.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 41 , Issue 2 , March 1979 , pp. 263 - 274
Copyright
Copyright © The Nutrition Society 1979

References

Åkesson, B., Elovson, J. & Arvidson, G. (1970). Biochim. Biophys. Acta 210, 15.Google Scholar
Åkesson, B., Fehling, C. & Jägerstad, M. (1978). Br. J. Nufr. 40, 521.Google Scholar
Alling, C., Bruce, Å., Karlsson, I. & Svennerholm, L. (1974 a). J. Neurochem. 23, 1263.Google Scholar
Alling, C., Bruce, Å., Karlsson, I. & Svennerholm, L. (1974 b). Nufr. Merab. 16, 38.Google Scholar
Alling, C., Bruce, Å., Karlsson, I. & Svennerholm, L. (1976). Nutr. Metab. 20, 440.Google Scholar
Alling, C. & Karlsson, I. (1973). J. Neurochem. 21, 1051.CrossRefGoogle Scholar
Ansell, G. B. & Metcalf, R. F. (1971). J. Neurochem. 18, 647.CrossRefGoogle Scholar
Ansell, G. B. & Spanner, S. (1971). Biochem. J. 122, 741.CrossRefGoogle Scholar
Belfrage, P., Wiebe, T. & Lundquist, A. (1970). Scund. J. clin. Lab. Invest. 26, 53.CrossRefGoogle Scholar
Benton, J. W., Moser, H. W., Dodge, P. R. & Carr, S. (1966). Pediatrics, Springfield 38, 801.Google Scholar
Berg Hansen, I. & Clausen, J. (1969). Z. ErnährWiss 9, 278.CrossRefGoogle Scholar
Björnstad, P. & Bremer, J. (1966). J. Lipid Res. 7, 38.Google Scholar
Cardinale, G. J., Carty, T. J. & Abeles, R. H. (1970). J. biol. Chem. 245, 3771.CrossRefGoogle Scholar
Dobbing, J. & Widdowson, E. M. (1965). Brain 88, 357.CrossRefGoogle ScholarPubMed
Fehling, C., Jägerstad, M., åkesson, B., Axelsson, J. & Brun, A. (1978). Br. J. Nutr. 39, 501.CrossRefGoogle Scholar
Fehling, C., Jägerstad, M. & Arvidson, G. (1978). Nutr. Metabol. 22, 82.CrossRefGoogle Scholar
Finkelstein, J. D., Kyle, W. E. & Harris, B. J. (1971). Archs Biochem. Biophys. 146, 84.Google Scholar
Fish, M. B., Pollycove, M. & Wallerstein, R. O. (1968). J. Lab. clin. Med. 72, 767.Google Scholar
Frenkel, E. P. & Kitchens, R. L. (1977). Proc. Soc. exp. Biof. Med. 156, 151.Google Scholar
Frenkel, E. P., Kitchens, R. L., Hersch, L. B. & Frenkel, R. (1974). J. biol. chem. 249, 6984.Google Scholar
Frenkel, E. P., Kitchens, R. L. & Johnston, J. M. (1973). J. biol. Chem. 248, 7540.Google Scholar
Frenkel, E. P., Mukherjee, A., Hackenbrock, C. R. & Srere, P. A. (1976). J. biol. Chem. 251, 2147.Google Scholar
Greenfield, J. G. & O'Flynn, E. (1933). Lancet ii, 62.CrossRefGoogle Scholar
Herbert, V. (1976). Present Knowledge in Nutrition p. 191. Washington DC: The Nutrition Foundation.Google Scholar
Kewitz, H. & Pleul, O. (1976). Proc. nut Acad. Sci. U.S.A. 73, 2181.Google Scholar
Kisic, A. & Rapport, M. M. (1974). J. Lipid Res. 15, 179.Google Scholar
Laird, R. D., McCormick, H. M. & Drill, V. A. (1965). Toxic. Appl. Pharmac. 7, 247.CrossRefGoogle Scholar
Lombardi, B., Pani, P. & Schlunk, F. F. (1969). J. Lipid Res. 9, 437.Google Scholar
McMurray, W. C. (1964). J. Neurochem. 11, 287.Google Scholar
Marston, H. R., Allen, S. H. & Smith, R. M. (1961). Nature, Lond. 190, 1087.Google Scholar
Morgenstern, R. D. & Abdel-Latif, A. A. (1974). J. Neurobiol. 5, 393.Google Scholar
Pant, S. S., Asbury, A. K. & Richardson, E. P. (1968). Acta Neurol. scand. 44, Suppl.35.Google Scholar
Porcelatti, G. & Pirotta, M. G. (1970). Enzymologia 38, 351.Google Scholar
Russell, J. S. R., Batten, F. E. & Collier, J. (1900). Brain 23, 39.Google Scholar
Sundler, R. & Åkesson, B. (1975 a). J. Biol. Chem. 250, 3359.Google Scholar
Sundler, R. & Åkesson, B. (1975 b). Biochem. J. 146, 309.Google Scholar
Tuma, D. J., Barak, A. J. & Sorrell, M. F. (1975). Biochem. Pharmac. 24, 1327.Google Scholar
Turner, D. A. & Cevallos, W. H. (1968). Clin. Biochem. 2, 1.Google Scholar
Volpe, J. J. & Kishimoto, Y. (1972). J. Neurochem. 19, 737.Google Scholar
Volpe, J. J. & Marasa, J. C. (1975). J. Neurochem. 25, 333.Google Scholar
Volpe, J. J. & Vagelos, P. R. (1973). Biochim. biophys. Acta 326, 293.Google Scholar
Wells, M. A. & Dittmer, J. C. (1967). Biochemistry 6, 3169.Google Scholar