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Lipid accumulation in obese Zucker rats is reduced by inclusion of raw kidney bean (Phaseolus vulgaris) in the diet

Published online by Cambridge University Press:  09 March 2007

A. Pusztai ,
G. Grant ,
W. C. Buchan ,
S. Bardocz ,
A. F. F. U. de Carvalho  and
S. W. B. Ewen
A. Pusztai*
Affiliation:
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK
G. Grant
Affiliation:
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK
W. C. Buchan
Affiliation:
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK
S. Bardocz
Affiliation:
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK
A. F. F. U. de Carvalho
Affiliation:
Department of Biology, Universidade Federal do Ceara, 60 001 Fortaleza (CE), Brazil
S. W. B. Ewen
Affiliation:
Department of Pathology, Aberdeen University Medical School, Aberdeen AB1 2ZX, UK
*
*Corresponding author:Dr A. Pusztai, fax +44 (0)1224 716616, email [email protected]
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Abstract

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The effects of inclusion of different levels of raw kidney bean (Phaseolus vulgaris) of high lectin content (27 g/kg meal) in a high-quality (lactalbumin) control diet were tested in nutritional trials on the growth and metabolism of obese Zucker (fafa) rats and their lean littermates in comparison with pair-fed controls. All diets contained 100 g total protein/kg and either 50 g lipids/kg (low fat) or 150 g lipids/kg (moderate fat). The growth of both obese and lean rats on bean diets was retarded by the daily bean intake in a dose-dependent manner. However, most of this was because bean-fed rats contained less body fat than the controls after 10 d. Thus, after feeding low-fat diets containing up to 130 g kidney bean/kg (lectin intake ≤ 0·2 g/kg body weight (BW) per d) in both 10 d and 70 d trials, the bodies of obese rats contained less fat but not protein than their pair-fed controls. Moreover, by increasing the lipid content of the diet to 150 g/kg, the level of bean inclusion could be increased to 280 g/kg (lectin intake ≥ 0·4 g/kg BW per d) without loss of body protein and skeletal muscle. Although these rats contained more body fat than those which were fed on low-fat diets, their weight reduction could be accounted for exclusively by reduced lipid content. In contrast, significant body protein loss occurred when the same diet of high lectin content was fed to lean littermates. Plasma insulin levels were significantly depressed in the obese Zucker rats on bean diets but the pancreas was not significantly enlarged nor its insulin content changed in 10 d trials. However, significant pancreatic growth occurred on long-term (70 d) bean feeding compared with pair-fed controls. The results suggest that, in addition to animal nutrition, it may also be possible to use the bean lectin as a dietary adjunct or therapeutic agent to stimulate gut function and ameliorate obesity if a safe and effective dose-range can be established for human subjects.

Keywords

Kidney beanObesityMuscleBody composition
Type
General Nutrition
Information
British Journal of Nutrition , Volume 79 , Issue 2 , February 1998 , pp. 213 - 221
Copyright
Copyright © The Nutrition Society 1998

References

Banwell, JG, Howard, R, Kabir, I, Adrian, TE, Diamond, RH & Abramowsky, C (1995) Small intestinal growth caused by feeding red kidney bean phytohemagglutinin lectin to rats. Gastroenterology 104, 1669–1677.CrossRefGoogle Scholar
Bardocz, S, Ewen, SWB & Pusztai, A (1993) Binding and endocytosis of Phaseolus vulgaris L4 isolectin and insulin by 3T3 and L6 cells – effects on protein synthesis. In Lectins: Biology–Biochemistry–Clinical Biochemistry, Vol. 8, pp. 258–264 [Driessche, Van E, Franz, H, Beeckmans, S, Pfuller, U, Kallikorm, A, and Bog-Hansen, TC, editors]. Hellerup, Denmark: Textop.Google Scholar
Bardocz, S, Grant, G, Pusztai, A, Franklin, MF & Carvalho, A de FFU (1996) The effect of phytohaemagglutinin at different dietary concentrations on the growth, body composition and plasma insulin of the rat. British Journal of Nutrition 76, 613–626.CrossRefGoogle ScholarPubMed
Bray, GA (1977) The Zucker-fatty rat: a review. Federation Proceedings 36, 148–153.Google ScholarPubMed
Carvalho, A de FFU (1993) Dietary kidney bean lectins affect insulin levels, change gene expression and modulate metabolism. DPhil. Thesis, University of Aberdeen.Google Scholar
Coates, ME, Donaghue, PN, Payne, PR & Ward, RJ (1969) Laboratory Animals Handbooks 2. Dietary Standards for Laboratory Rats and Mice [Coates, ME, Donaghue, PN, Payne, PR and Ward, RJ, editors]. London: London Laboratory Animals Ltd.Google Scholar
Grant, G, de Oliveira, JTA, Dorward, PM, Annand, MG, Waldron, M & Pusztai, A (1987) Metabolic and hormonal changes in rats resulting from consumption of kidney bean (Phaseolus vulgaris) or soyabean (Glycine max). Nutritional Reports International 36, 763–772.Google Scholar
Grant, G, Dorward, PM, Buchan, WC, Armour, JC & Pusztai, A (1995) Consumption of diets containing raw soya beans (Glycine max), kidney beans (Phaseolus vulgaris), cowpeas (Vigna unguiculata) or lupin seeds (Lupinus angustifolius) by rats for up to 700 days: effects on body composition and organ weights. British Journal of Nutrition 73, 17–29.CrossRefGoogle ScholarPubMed
Grant, G, Dorward, PM & Pusztai, A (1993) Pancreatic enlargement is evident in rats fed diets containing raw soyabean (Glycine max) or cowpea (Vigna unguiculata) for 800 days but not in those given diets based on kidney bean (Phaseolus vulgaris) or lupinseed (Lupinus angustifolius). Journal of Nutrition 123, 2207–2215.CrossRefGoogle ScholarPubMed
Grant, G, Henderson, LT, Edwards, JE, Ewan, EC, Bardocz, S & Pusztai, A (1997) Kidney bean and soybean lectins cause enzyme secretion by pancreatic acini in vitro. Life Sciences 60, 1589–1595.CrossRefGoogle ScholarPubMed
Grant, G, McKenzie, NH, Watt, WB, Stewart, JC, Dorward, PM & Pusztai, A (1986) Nutritional evaluation of soya beans (Glycine max): nitrogen balance and fractionation studies. Journal of the Science of Food and Agriculture 37, 1001–1010.CrossRefGoogle Scholar
Herzig, KH, Bardocz, S, Grant, G, Nustede, R, Fölsch, U & Pusztai, A (1997) Red kidney bean lectin is a potent CCK releasing stimulus inducing pancreatic growth. Gut 41, 333–338.CrossRefGoogle ScholarPubMed
James, WPT (1992) Epidemiology of obesity. International Journal of Obesity 16, Suppl., 87–97.Google Scholar
King, TP, Pusztai, A & Clarke, EMW (1980) Immunocytochemical localization of ingested kidney bean (Phaseolus vulgaris) lectins in rat gut. Histochemical Journal 12, 201–208.CrossRefGoogle ScholarPubMed
Knott, RM, Grant, G, Bardocz, S, Pusztai, A, Carvalho A de, FFU & Hesketh, JE (1992) Alterations in the level of insulin receptor and Glut-4 mRNA in skeletal muscle from rats fed a kidney bean (Phaseolus vulgaris) diet. International Journal of Biochemistry 24, 897–902.CrossRefGoogle ScholarPubMed
Kreif, S & Bazin, R (1991) Genetic obesity: Is the defect in the sympathetic nervous system? A review through developmental studies in the preobese Zucker rat. Proceedings of the Society for Experimental Biology and Medicine 198, 528–538.CrossRefGoogle Scholar
McLaughlin, CL, Peiken, SR & Baile, CA (1982) Decreased pancreatic exocrine response to cholecystokinin in Zucker obese rats. American Journal of Physiology 242, G612–G619.Google ScholarPubMed
Martin-Cabrejas, MA, Esteban, RM, Waldron, KW, Maina, G, Grant, G, Bardocz, S & Pusztai, A (1995) Hard-to-cook phenomenon in beans: changes in antinutrient factors and nitrogenous compounds during storage. Journal of the Science of Food and Agriculture 69, 429–435.CrossRefGoogle Scholar
Melmed, RN, Turner, RC & Holt, SJ (1973) Intermediate cells of the pancreas II. The effects of dietary soyabean trypsin inhibitor on acinar-β cell structure and function in the rat. Journal of Cell Science 13, 279–295.CrossRefGoogle ScholarPubMed
Proietto, J & Thorburn, AW (1994) Animal models of obesity. Theories of aetiology. Baillieres Clinical Endocrinology and Metabolism 8, 509–525.CrossRefGoogle ScholarPubMed
Pusztai, A (1991) Plant Lectins. Cambridge: Cambridge University Press.Google Scholar
Pusztai, A, Clarke, EMW, King, TP & Stewart, JC (1979) Nutritional evaluation of kidney beans (Phaseolus vulgaris): chemical composition, lectin content and nutritional value of selected cultivars. Journal of the Science of Food and Agriculture 30, 843–848.CrossRefGoogle ScholarPubMed
Pusztai, A, Ewen, SWB, Carvalho, A de, FFU, Grant G, Stewart, JC & Bardocz, S (1991) Immune and hormonal effects of dietary lectins. In European Food Toxicology III. Proceedings of an Interdisciplinary Conference on the Effects of Food on the Immune and Hormonal Systems, pp. 20–24. Zurich, Switzerland: University of Zurich.Google Scholar
Pusztai, A, Ewen, SWB, Grant, G, Brown, DS, Peumans, WJ, Van Damme, EJM & Bardocz, S (1992) Stimulation of growth and polyamine accretion in the small intestine and pancreas by lectins and trypsin inhibitors. In Falk Symposium 62: Polyamines in the Gastrointestinal Tract, pp. 473–483. Dordrecht, Boston, London: Kluver Academic Press.Google Scholar
Pusztai, A, Ewen, SWB, Grant, G, Peumans, WJ, van Damme, EJM, Coates, ME & Bardocz, S (1995) Lectins and also bacteria modify the glycosylation of gut receptors in the rat. Glyco-conjugate Journal 12, 22–35.CrossRefGoogle ScholarPubMed
Pusztai, A, Ewen, SWB, Grant, G, Peumans, WJ, van Damme, EJM, Rubio, L & Bardocz, S (1990) Relationship between survival and binding of plant lectins during small intestinal passage and their effectiveness as growth factors. Digestion 46, Suppl. 2, 308–316.CrossRefGoogle Scholar
Pusztai, A, Grant, G, Spencer, RJ, Duguid, TJ, Brown, DS, Ewen, SWB, Peumans, WJ, Van Damme, EJM & Bardocz, S (1993) Kidney bean lectin-induced Escherichia coli overgrowth in the small intestine is blocked by GNA, a mannose-specific lectin. Journal of Applied Bacteriology 75, 360–368.CrossRefGoogle ScholarPubMed
Pusztai, A, Greer, F & Grant, G (1989) Specific uptake of dietary lectins into the systemic circulation of rats. Biochemical Society Transactions 17, 481–482.CrossRefGoogle Scholar
Pusztai, A & Watt, WB (1974) Isolectins of Phaseolus vulgaris: a comprehensive study of fractionation. Biochimica et Biophysica Acta 365, 57–71.CrossRefGoogle ScholarPubMed
Trinder, P (1967) Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of Clinical Biochemistry 6, 24–27.CrossRefGoogle Scholar