Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-28T13:47:34.507Z Has data issue: false hasContentIssue false
  • English
  • Français

Lipid absorption and intestinal tumour incidence in rats fed on varying levels of calcium and butterfat

Published online by Cambridge University Press:  09 March 2007

A. R. Behling ,
S. M. Kaup ,
L. L. Choquette  and
J. L. Greger
A. R. Behling
Affiliation:
Department of Nutritional Sciences, University of Wisconsin, 1415 Linden Drive, Madison WI 53706, USA
S. M. Kaup
Affiliation:
Department of Nutritional Sciences, University of Wisconsin, 1415 Linden Drive, Madison WI 53706, USA
L. L. Choquette
Affiliation:
Department of Nutritional Sciences, University of Wisconsin, 1415 Linden Drive, Madison WI 53706, USA
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The purpose of the 2 × 2 factorial study was to determine the effect of varying levels of dietary calcium (2.5 and 10 g/kg) and butterfat (50 and 200 g/kg) on lipid utilization and on development of colon tumours in animals initiated with 1,2-dimethylhydrazine dihydrochloride. Among rats fed on 200 g butterfat/kg, the fourfold increase in Ca intake induced more than a sevenfold increase in faecal excretion of total lipids and almost a fortyfold increase in faecal excretion of acid-extractable lipid. Among rats fed on 50 g butterfat/kg, the ingestion of supplemental Ca had a less dramatic effect and induced only a twofold increase in faecal excretion of total lipids and a threefold increase in acid-extractable lipid. The volume of intestinal adenocarcinomas was correlated with the excretion of acid-extractable lipid in faeces (R 0.369, P < 0.02). Caecal enzymic activity was not correlated with tumour incidence or size or faecal lipid excretion. Overall, the fourfold increase in Ca intakes decreased total lipid absorption significantly but by less than 6%.

Keywords

CalciumLipid absorptionButterfatRat
Type
Diet and Lipid Metabolism
Information
British Journal of Nutrition , Volume 64 , Issue 2 , September 1990 , pp. 505 - 513
Copyright
Copyright © The Nutrition Society 1990

References

American Institute of Nutrition (1977). Report of the American Institute of Nutrition ad hoc committee on standards for nutrition studies. Journal of Nutrition 107, 1340–1348.Google Scholar
Appleton, G. V. N., Davies, P. W., Bristol, J. B. & Williamson, R. C. N. (1987). Inhibition of intestinal carcinogenesis by dietary supplementation with calcium. British Journal of Surgery 74, 523–535.CrossRefGoogle ScholarPubMed
Behling, A. R. & Greger, J. L. (1988). Mineral metabolism of aging female rats fed various commercially available calcium supplements or yogurt. Pharmacological Research 5, 501–505.Google Scholar
Behling, A. R., Kaup, S. M. & Greger, J. L. (1990). Changes in intestinal function of rats initiated with DMH and fed varying levels of butterfat, calcium and magnesium. Nutrition and Cancer 31, 189–199.Google Scholar
Bligh, E. G. & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37, 911–917.CrossRefGoogle ScholarPubMed
Bull, A., Bird, R. P., Bruce, W. R., Nigro, N. & Medline, A. (1987). Effect of calcium on azoxymethane induced tumors in rats. Gastroenterology 92, 1332.Google Scholar
Drenick, E. J. (1961). The influence of ingestion of calcium and other soap-forming substances on fecal fat. Gasiroenterology 41, 242–244.Google Scholar
Felder, R. M. & Kosseau, R. W. (1978). Fundamentals of material balances. Elementary Principles of Chemical Processes, pp. 81–164. New York: John Wiley & Sons.Google Scholar
Freeman, H. F. (1986). Effects of differing purified cellulose, pectin, and hemicellulose fiber diets on fecal enzymes in 1,2-dimethylhydrazine-induced rat colon carcinogenesis. Cancer Research 46, 5529–5532.Google Scholar
Fleischman, A. I., Yacowitz, H., Hayton, T. & Bierenbaum, M. L. (1966). Effects of dietary calcium upon lipid metabolism in mature male rats fed beef tallow. Journal of Nutrition 88, 255–260.CrossRefGoogle ScholarPubMed
Goldin, B. R. & Gorhach, S. L. (1981). Effect of antibiotics on incidence of rat intestinal tumors induced by 1,2-dimethylhydrazine dihydrochloride. Journal of the National Cancer Institute 67, 877–880.Google Scholar
Gregoire, R. C., Stern, H. S., Yeung, K. S., Stadler, J., Langley, S., Furrer, R. & Bruce, W. R. (1989). Effect of calcium supplementation on mucosal cell proliferation in high risk patients for colon cancer. Gut 30, 376–382.Google Scholar
Kasprzak, K. S. & Waalkes, M. P. (1986). The role of calcium, magnesium and zinc in carcinogenesis. In Essential Nutrients in Carcinogenesis, pp. 497–515 [Poirier, L. A., Neuterne, P. M. and Pariza, M. W., editors]. New York: Plenum Press.CrossRefGoogle Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 165–275.Google Scholar
Nauss, K. M., Jacobs, L. R. & Newberne, P. M. (1987). Dietary fat and fiber: relationship to caloric intake, body growth, and colon tumorigenesis. American Journal of Clinical Nutrition 45, 243–251.Google Scholar
Newmark, H. L., Wargovich, M. J. & Bruce, W. R. (1984). Colon cancer and dietary fat, phosphate, and calcium: a hypothesis. Journal of the National Cancer Institute 72, 1323–1325.Google ScholarPubMed
Pence, B. C. & Buddingh, F. (1988). Inhibition of dietary fat-promoted colon carcinogenesis in rats by supplemental calcium or vitamin D3. Carcinogenesis 9, 187–190.CrossRefGoogle ScholarPubMed
Reddy, B. S., Mangat, S., Weisburger, J. H. & Wynder, E. L. (1977). Effect of high-risk diets for colon carcinogenesis on intestinal, mucosal and bacterial β-glucuronidase activity in F344 rats. Cancer Research 37, 3533–3536.Google ScholarPubMed
Rozhin, J., Wilson, P. S., Bull, A. W. & Nigro, N. D. (1984). Ornithine decarboxylase activity in the rat and human colon. Cancer Research 44, 3226–3230.Google ScholarPubMed
SAS Institute Inc. (1985). SAS/STATTM Guide for Personal Computers, version 6 ed., pp. 183–260. Cary, NC: SAS Institute Inc.Google Scholar
Shiau, S.-Y. & Chang, G. W. (1983). Effects of dietary fiber on fecal mucinase and β-glucuronidase activity in rats. Journal of Nutrition 113, 138–144.CrossRefGoogle ScholarPubMed
Slattery, M. L., Sorenson, A. W. & Ford, M. H. (1988). Dietary calcium intake as a mitigating factor in colon cancer. American Journal of Epidemiology 128, 504–514.Google Scholar
Sukhija, P. S. & Palmquist, D. L. (1988). Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry 36, 1202–1206.Google Scholar
Ward, J. M. (1974). Morphogenesis of chemically induced neoplasms of the colon and small intestine in rats. Laboratory Investigation 30, 505–513.Google Scholar
Wargovich, M. J. (1988). Calcium and colon cancer. Journal of American College of Nutrition 7, 295–300.CrossRefGoogle ScholarPubMed
Wargovich, M. J., End, V. W. S., Newmark, H. L. & Bruce, W. R. (1983). Calcium ameliorates the toxic effect of deoxycholic acid on colonic epithelium. Carcinogenesis 4, 1205–1207.CrossRefGoogle ScholarPubMed
Willett, W. C. & MacMahon, B. (1984). Diet and cancer – an overview, part 2. New England Journal of Medicine 310, 697–703.Google Scholar
Wise, A., Mallett, A. K. & Rowland, I. R. (1982). Dietary fibre, bacterial metabolism and toxicity of nitrate in the rat. Xenobiotica 12, 111–118.CrossRefGoogle ScholarPubMed