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Lactobacillus gasseri: effects on mouse intestinal flora enzyme activity and isoflavonoids in the caecum and plasma

Published online by Cambridge University Press:  09 March 2007

Motoi Tamura ,
Mayumi Ohnishi-Kameyama  and
Kazuki Shinohara
Motoi Tamura*
Affiliation:
National Food Research Institute, Tsukuba 305-8642, Japan
Mayumi Ohnishi-Kameyama
Affiliation:
National Food Research Institute, Tsukuba 305-8642, Japan
Kazuki Shinohara
Affiliation:
National Food Research Institute, Tsukuba 305-8642, Japan
*
*Corresponding author: Dr Motoi Tamura, fax +81 298 38 7996, email, [email protected]
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Abstract

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The effects of Lactobacillus gasseri JCM 1131T on isoflavonoid levels within the caecum and plasma were assessed in adult mice. Male 5-week-old mice were fed an AIN 93M diet for 30 d. Two groups of mice were administered either L. gasseri JCM 1131T (the LGI group) or physiological saline solution (the control (CI) group) daily for 5 d before dissection. The plasma daidzein concentration was significantly higher in the LGI group, however, their plasma equol concentration was significantly less than in the CI group. The total amount of equol present as aglycone in the caecum was significantly greater in the CI group, but there was no significant difference in the total daidzein present as caecal aglycone. In an in vitro incubation of daidzin with the faecal flora of mice, the equol concentration was significantly higher in the CI group. The numbers of lactobacilli present were significantly higher in the LGI group. The present data suggest that the administration of L. gasseri is likely to influence the effect of isoflavonoids on the host via changes in the gastrointestinal environment.

Keywords

DaidzeinEquolLactobacillus gasseriCaecal enzyme activity
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 5 , November 2004 , pp. 771 - 776
Copyright
Copyright © The Nutrition Society 2004

References

Aleljung, P, Paulsson, M, Emody, L, Andersson, M, Naidu, AS & Wadström, T (1991) Collagen binding by lactobacilli. Curr Microbiol 23, 33–38CrossRefGoogle Scholar
Anthony, MS, Clarkson, TB & Williams, JK (1998) Effects of soy isoflavones on atherosclerosis: potential mechanisms. Am J Clin Nutr 68, 1390S – 1393SCrossRefGoogle ScholarPubMed
Blair, RM, Appt, SE, Franke, AA & Clarkson, TB (2003) Treatment with antibiotics reduces plasma equol concentration in cynomolgus monkeys ( Macaca fascicularis ). J Nutr 133, 2262–2267CrossRefGoogle ScholarPubMed
Bowey, E, Adlercreutz, H & Rowland, I (2003) Metabolism of isoflavones and lignans by the gut microflora: a study in germ-free and human flora associated rats. Food Chem Toxicol 41, 631–636CrossRefGoogle ScholarPubMed
Cruz, ML, Wong, WW, Mimouni, F, Hachey, DL, Setchell, KD, Klein, PD & Tsang, RC (1994) Effects of infant nutrition on cholesterol synthesis rates. Pediatr Res 35, 135–140CrossRefGoogle ScholarPubMed
Fuller, R (1989) A review: probiotics in man and animals. J Appl Bacteriol 66, 365–378Google Scholar
Gilliland, SE (1990) Health and nutritional benefits from lactic acid bacteria. FEMS Microbiol Rev 7, 175–188CrossRefGoogle ScholarPubMed
Hammes, WP & Tichaczek, PS (1994) The potential of lactic acid bacteria for the production of safe and wholesome food. Z Lebensm Unters Forsch 198, 193–201CrossRefGoogle ScholarPubMed
Isolauri, E, Juntunen, M, Rautanen, T, Sillanaukee, P & Koivula, T (1991) A human Lactobacillus strain ( Lactobacillus casei sp strain GG) promotes recovery from acute diarrhea in children. Pediatrics 88, 90–97Google ScholarPubMed
Izumi, T, Piskula, MK, Osawa, S, Obata, A, Tobe, K, Saito, M, Kataoka, S, Kubota, Y & Kikuchi, M (2000) Soy isoflavone aglycones are absorbed faster and in higher amounts than their glucosides in humans. J Nutr 130, 1695–1699CrossRefGoogle ScholarPubMed
Lampe, JW, Karr, SC, Hutchins, AM & Slavin, JL (1998) Urinary equol excretion with a soy challenge: influence of habitual diet. Proc Soc Exp Biol Med 217, 335–339CrossRefGoogle ScholarPubMed
Lieberman, S (1996) Are the differences between estradiol and other estrogens, naturally occurring or synthetic, merely semantical? J Clin Endocrinol Metab 81, 850–851CrossRefGoogle ScholarPubMed
Morotomi, M & Mutai, M (1986) In vitro binding of potent mutagenic pyrolysates by intestinal bacteria. J Natl Cancer Inst 77, 195–201Google ScholarPubMed
Ouwehand, AC (1998) Antimicrobial components from lactic acid bacteria. In Lactic Acid Bacteria: Microbial Ecology and Functional Aspects, 2nd ed. pp. 139–159 [Salminen, S and Wright von, A, editors]. New York: Marcel Dekker Inc.Google Scholar
Pedrosa, MC, Golner, BB, Goldin, BR, Barakat, S, Dallal, GE & Russell, RM (1995) Survival of yogurt-containing organisms and Lactobacillus gasseri (ADH) and their effect on bacterial enzyme activity in the gastrointestinal tract of healthy and hypochlorhydric elderly subjects. Am J Clin Nutr 61, 353–359CrossRefGoogle ScholarPubMed
Reeves, PG, Nielsen, FH & Fahey, GC Jr (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123, 1939–1951CrossRefGoogle Scholar
Rowland, IR, Mallett, AK & Wise, A (1983) A comparison of the activity of five microbial enzymes in cecal content from rats, mice, and hamsters, and response to dietary pectin. Toxicol Appl Pharmacol 69, 143–148CrossRefGoogle ScholarPubMed
Rowland, IR, Wiseman, H, Sanders, TA, Adlercreutz, H & Bowey, EA (2000) Interindividual variation in metabolism of soy isoflavones and lignans: influence of habitual diet on equol production by the gut microflora. Nutr Cancer 36, 27–32CrossRefGoogle ScholarPubMed
Salminen, E, Elomaa, I, Minkkinen, J, Vapaatalo, H & Salminen, S (1988) Preservation of intestinal integrity during radiotherapy using live Lactobacillus acidophilus cultures. Clin Radiol 39, 435–437CrossRefGoogle ScholarPubMed
Salminen, S, Isolauri, E & Salminen, E (1996) Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful strains and future challenges. Antonie Van Leeuwenhoek 70, 347–358CrossRefGoogle ScholarPubMed
Saxelin, M, Elo, S, Salminen, S & Vapaatalo, H (1991) Dose response colonization of faeces after oral administration of Lactobacillus casei strain GG. Microbiol Ecol Health Dis 4, 209–214CrossRefGoogle Scholar
Setchell, KD, Borriello, SP, Hulme, P, Kirk, DN & Axelson, M (1984) Nonsteroidal estrogens of dietary origin: possible roles in hormone-dependent disease. Am J Clin Nutr 40, 569–578CrossRefGoogle ScholarPubMed
Setchell, KD, Brown, NM & Lydeking-Olsen, E (2002 a) The clinical importance of the metabolite equol - a clue to the effectiveness of soy and its isoflavones. J Nutr 132, 3577–3584CrossRefGoogle Scholar
Setchell, KD, Brown, NM, Zimmer-Nechemias, L, Brashear, WT, Wolfe, BE, Kirschner, AS & Heubi, JE (2002 b) Evidence for lack of absorption of soy isoflavone glycosides in humans, supporting the crucial role of intestinal metabolism for bioavailability. Am J Clin Nutr 76, 447–453CrossRefGoogle ScholarPubMed
Setchell, KD, Zimmer-Nechemias, L, Cai, J & Heubi, JE (1997) Exposure of infants to phyto-oestrogens from soy-based infant formula. Lancet 350, 23–27CrossRefGoogle ScholarPubMed
Shutt, DA & Cox, RI (1972) Steroid and phyto-oestrogen binding to sheep uterine receptors in vitro. J Endocrinol 52, 299–310CrossRefGoogle ScholarPubMed
Sims, W (1964) The effect of mucin on the survival of lactobacilli and streptococci. J Gen Microbiol 37, 335–340CrossRefGoogle ScholarPubMed
Tamura, M, Hirayama, K & Itoh, K (1996) Comparison of colonic bacterial enzymes in gnotobiotic mice monoassociated with different intestinal bacteria. Microbiol Ecol Health Dis 9, 287–294CrossRefGoogle Scholar
Zhu, WM, Liu, W & Wu, DQ (2000) Isolation and characterization of a new bacteriocin from Lactobacillus gasseri KT7. J Appl Microbiol 88, 877–886CrossRefGoogle ScholarPubMed