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Iron availability of a fortified processed wheat cereal: a comparison of fourteen iron forms using an in vitro digestion/human colonic adenocarcinoma (CaCo-2) cell model

Published online by Cambridge University Press:  08 March 2007

Gary Wortley ,
Steven Leusner ,
Carolyn Good ,
Eric Gugger  and
Raymond Glahn
Gary Wortley
Affiliation:
The Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK
Steven Leusner
Affiliation:
Ottens Flavors, 7800 Holstein Avenue, Philadelphia, PA 19153, USA
Carolyn Good
Affiliation:
General Mills, Bell Institute of Health & Nutrition, 9000 Plymouth Avenue North, Minneapolis, MN 55427, USA
Eric Gugger
Affiliation:
General Mills, Bell Institute of Health & Nutrition, 9000 Plymouth Avenue North, Minneapolis, MN 55427, USA
Raymond Glahn*
Affiliation:
US Department of Agriculture – Agricultural Research Service, US Plant, Soil & Nutrition Laboratory, Tower Road, Ithaca, NY 14853, USA
*
*Corresponding author: Dr R. Glahn, fax +1 607 2551132, email [email protected]
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Abstract

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In this three-phase study we first compared the availability of fourteen Fe forms in a wheat-based ready-to-eat breakfast cereal using an in vitro digestion/human colonic adenocarcinoma (CaCo-2) cell model. We then investigated the effect of milk and/or coffee on those fortified cereals found in phase 1 to show promising increases in Fe availability. The Fe forms assessed in phase 1 were reduced (control), carbonyl, electrolytic, FePO4, FeSO4, FeCO3, Na2FeEDTA, Ferrochel® (Albion Laboratories, Clearfield, UT, USA; ferrous bis-glycinate), encapsulated ferrous fumarate, FeSO4, ferrous lactate and Biofer® (LipoTech, Britwell Salome, Oxfordshire; FeSO4), SQM® (Sea-Questra-Min Iron; Quali Tech, Chaska, MN, USA; polysaccharide-complexed FeSO4) and Sun Active® (Taiyo Kagaku, Yokkaichi, Japan). All these forms increased Fe uptake compared with the unfortified cereal. Relative to the control, the following increases in Fe availability were observed: electrolytic, 52 %; ferrous fumarate, 30–35 %; Sun Active, 78 %; Ferrochel, 125 %; Na2FeEDTA, 291 %. Recent human studies have shown similar data with regard to Ferrochel, FeSO4 and Na2FeEDTA, with the latter being more bioavailable. Our phase-2 studies indicated that the addition of milk to FeSO4-fortified cereal increased Fe availability, but this availability was markedly decreased by the addition of coffee to the digest. Conversely, a loss in availability from Na2FeEDTA was observed with the addition of milk; however, the addition of coffee did not markedly affect Fe availability from this form. In phase-3 studies we observed increased Fe availability upon the addition of milk to cereals containing Ferrochel, FeSO4, Sun Active, a mixture of reduced Fe and Na2FeEDTA or reduced Fe. For these forms we did not assess the behaviour after the addition of coffee. In conclusion, when considering possible fortificants for optimizing Fe bioavailability within a foodstuff, it is of paramount importance to consider the interaction between the fortified foodstuffs and other components of the meal (such as milk and coffee with a breakfast).

Keywords

Iron availabilityIn vitro digestionCaCo-2Wheat cerealsIron fortification
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 1 , January 2005 , pp. 65 - 71
Copyright
Copyright © The Nutrition Society 2005

References

Alvarez-Hernandez, X, Nichols, G & Glass, J (1991) Caco-2 cell line: a system for studying intestinal iron transport across epithelial cell monolayers. Biochem Biophys Acta 1070, 205–208.CrossRefGoogle Scholar
Fairweather-Tait, SJ, Wortley, GM, Teucher, B & Dainty, J (2000) Iron absorption from a breakfast cereal: effects of EDTA compounds and ascorbic acid. Int J Vitam Nutr Res 70, 117–122.Google Scholar
Gangloff, MB, Lai, C, Van Campen, DR, Miller, DD, Norvell, WA & Glahn, RP (1996) Ferrous iron uptake but not transfer is down-regulated in Caco-2 cells grown in high iron serum-free medium. J Nutr 126, 3118–3127.CrossRefGoogle Scholar
Glahn, RP, Lee, OA, Yeung, A, Goldman, MI & Miller, DD (1998) Caco-2 cell ferritin formation predicts nonradiolabeled food iron availability in an in vitro digestion/Caco-2 cell culture model. J Nutr 128, 1555–1561.CrossRefGoogle Scholar
Glahn, R, Lee, OA & Miller, D (1999) In vitro digestion/Caco-2 cell culture model to determine optimal ascorbic acid to Fe ratio in rice cereal. J Food Sci 64, 925–928.CrossRefGoogle Scholar
Glahn, RP, Rassier, M, Goldman, MI, Lee, OA & Cha, J (2000) A comparison of iron availability from commercial iron preparations using an in vitro digestion/Caco-2 cell culture model. J Nutr Biochem 11, 62–68.CrossRefGoogle ScholarPubMed
Glahn, RP, Cheng, Z, Welch, RM & Gregorio, GB (2002) Comparison of iron bioavailability from 15 rice genotypes: studies using an in vitro digestion/Caco-2 cell culture model. J Agric Food Chem 50, 3586–3591.CrossRefGoogle ScholarPubMed
Han, O, Failla, ML, Hill, AD, Morris, ER & Smith, JC Jr (1994) Inositol phosphates inhibit uptake and transport of iron and zinc by a human intestinal cell line. J Nutr 124, 580–587.CrossRefGoogle ScholarPubMed
Harvey, LJ, Armah, CN, Dainty, JR, Foxall, RJ, Lewis, DJ, Langford, NJ & Fairweather-Tait, SSJ (2004) The impact of menstrual blood loss on risk of iron deficiency in women of childbearing age consuming omnivorous and vegetarian diets in the UK Am J Clin Nutr.CrossRefGoogle Scholar
Hoglund, S & Reizenstein, P (1969) Studies in iron absorption. V. Effect of gastrointestinal factors on iron absorption. Blood 34, 486–504.Google ScholarPubMed
Huebers, HA, Brittenham, GM, Csiba, E & Finch, CA (1986) Absorption of carbonyl iron. J Lab Clin Med 108, 473–478.Google ScholarPubMed
Hurrell, RF (2002) Fortification: overcoming technical and practical barriers. J Nutr 132 806S – 812S.CrossRefGoogle ScholarPubMed
Hurrell, RF, Lynch, SR, Trinidad, TP, Dassenko, SA & Cook, JD (1989) Iron absorption in humans as influenced by bovine milk proteins. Am J Clin Nutr 49, 546–552.CrossRefGoogle ScholarPubMed
Lynch, S, Bothwell, T, Hurrel, R & MacPhail, A (1993) Iron EDTA for Food Fortification.In Report of the International Nutritional Anemia Consultative Group (INACG), Washington, DC: ILSI Human Nutrition Institute.Google Scholar
MacPhail, AP & Bothwell, TH (1992) The prevalence and causes of nutritional iron deficiency anemia.In Nutritional Anemias Nestle Nutrition Workshop Series, 1–12 [Fomon, SJ, Zlotkin, S, editors]. New York: Raven Press.Google Scholar
MacPhail, AP, Patel, RC, Bothwell, TH & Lamparelli, RD (1994) EDTA and the absorption of iron from food. Am J Clin Nutr 59, 644–648.CrossRefGoogle ScholarPubMed
Miller, DD & Berner, LA (1989) Is solubility in vitro a reliable predictor of iron bioavailability?. Biol Trace Elem Res 19, 11–24.CrossRefGoogle ScholarPubMed
Motulsky, H (1995) Comparing three or more means Intuitive Biostatistics, 255–262 [Motulsky, H, editors] New York: Oxford University Press.Google Scholar
Olivares, M, Pizarro, F, Pineda, O, Name, JJ, Hertrampf, E & Walter, T (1997) Milk inhibits and ascorbic acid favors ferrous bis-glycine chelate bioavailability in humans. J Nutr 127, 1407–1411.CrossRefGoogle ScholarPubMed
Yun, S, Habicht, J-P, Miller, DD & Glahn, RP (2004) An in vitro digestion/Caco-2 cell culture system accurately predicts the effects of ascorbic acid and polyphenolic compounds on iron bioavailability in humans J Nutr.CrossRefGoogle Scholar