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Involvement of lipogenesis in the lower VLDL secretion induced by oligofructose in rats

Published online by Cambridge University Press:  09 March 2007

N. Kok ,
M. Roberfroid ,
A. Robert  and
N. Delzenne
N. Kok
Affiliation:
Unité de Biochimie Toxicologique et Cancérologique, Département des Sciences Pharmaceutiques, Université Catholigue de Louvain, BCTC 7369, Avenue Mounier 73, B-1200, Brussels, Belgium
M. Roberfroid
Affiliation:
Unité de Biochimie Toxicologique et Cancérologique, Département des Sciences Pharmaceutiques, Université Catholigue de Louvain, BCTC 7369, Avenue Mounier 73, B-1200, Brussels, Belgium
A. Robert
Affiliation:
Quantitative Methods in Health Sciences Section, Université Catholique de Louvain, Avenue Hippocrate 55, Box 5550, B-I200, Brussels, Belgium
N. Delzenne
Affiliation:
Unité de Biochimie Toxicologique et Cancérologique, Département des Sciences Pharmaceutiques, Université Catholigue de Louvain, BCTC 7369, Avenue Mounier 73, B-1200, Brussels, Belgium
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Abstract

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Dietary supplementation with oligofructose (OFS; 100 g/kg), a non-digestibleoligomer of β-D-fructose, decreases serum triacylglycerols in serum and VLDL of rats. In order to investigate the role of hepatic metabolism in the hypolipidaemic effect of OFS, male Wistar rats were fed on a standard diet with or without 1OOg Raftilose® P95/kg as OFS source for 30 d. OFS feeding (1) significantly decreased triacylglycerol and phospholipid concentrations in both blood and liver, (2) increased the glycerol-3-phosphate liver content but decreased the hepatic activity of glycerol-3-phosphate acyltransferase (EC 2.3.1.15), suggesting a decrease in acylglycerol synthesis, (3) did not affect the blood non-caterified fatty acid concentrations, but (4) reduced by 54% the capacity of isolated hepatocytesto synthesize and secrete triacylglycerols from labelled acetate; the activity of fatty acid synthase, a key lipogenic enzyme was also significantly decreased. These findings suggest that OFS decreases serum triacylglycerols by reducing de nova fatty acid synthesis in the liver; the lower insulin level in the serum of OFS-fed rats could explain, at least partly, the metabolic effect induced by such nondigestible carbohydrates.

Keywords

FructansLiverLipogenesisVery-low-density lipoproteins
Type
General Nutrition
Information
British Journal of Nutrition , Volume 76 , Issue 6 , December 1996 , pp. 881 - 890
Copyright
Copyright © The Nutrition Society 1997

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