Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-30T18:59:04.088Z Has data issue: false hasContentIssue false
  • English
  • Français

Interaction of fish oil and a glucocorticoid on metabolic responses to an oral glucose load in healthy human subjects

Published online by Cambridge University Press:  08 March 2007

Jacques Delarue ,
Chang-Hong Li ,
Richard Cohen ,
Charlotte Corporeau  and
Brigitte Simon
Jacques Delarue*
Affiliation:
Equipe d'Accueil 948 ‘Oxylipides’, Laboratoire Régional de Nutrition Humaine, Faculté de Médecine de Brest & CHU, F-29 200 Brest, France
Chang-Hong Li
Affiliation:
Jeune Equipe 313 ‘Lipides et Croissance’, Faculté de Médecine, F-37 000 ToursFrance
Richard Cohen
Affiliation:
Fédération de Biochimiebiologie spécialiséeimmunoanalyseHôpital Edouard HerriotF-69 000 LyonFrance
Charlotte Corporeau
Affiliation:
Equipe d'Accueil 948 ‘Oxylipides’, Laboratoire Régional de Nutrition Humaine, Faculté de Médecine de Brest & CHU, F-29 200 Brest, France
Brigitte Simon
Affiliation:
Equipe d'Accueil 948 ‘Oxylipides’Faculté de Médecine de BrestF-29 200 BrestFrance
*
*corresponding author: Professor Jacques Delarue, fax +33 2 98 34 78 82, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Compared with saturated fat, n-3 long-chain PUFA-rich fish oil improves insulin sensitivity in rats. We studied whether n-3 long-chain PUFA could prevent insulin resistance induced by dexamethasone (a glucocorticoid) in healthy human volunteers. A group of eight subjects was studied twice after a 2d dexamethasone treatment, before and after a 3-week supplementation with fish oil (providing daily doses of 1·1g 20:5n-3 and 0·7g 22:6n-3). The subjects were studied during the basal state and over the 6h following an oral glucose load (1g/kg). Plasma glucose fluxes were traced with [6,6-2H2]glucose and [13C]glucose (naturally 13C-enriched corn glucose). Substrate oxidation was obtained from indirect calorimetry. Following fish oil supplementation, plasma glucose fluxes and substrate oxidation were maintained despite a 17% reduction (P<0·05) in the area under the curve of plasma insulin response, suggesting an insulin-sensitizing effect.

Keywords

Eicosapentaenoic acidDocosahexaenoic acidPolyunsaturated fatty acidsStable isotopesInsulin resistance
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 2 , February 2006 , pp. 267 - 272
Copyright
Copyright © The Nutrition Society 2006

References

Bergmeyer, HU, Bergmeyer, J & Grassl, JMMethods of Enzymatic Analysis, 3rd ed., vol. VI. Weinheim: Verlag Chemie. 1977.Google Scholar
Borkman, M, Chisholm, DJ, Furler, SM, Storlien, LH, Kraegen, EW, Simons, LA & Chesterman, CNEffects of fish oil supplementation on glucose and lipid metabolism in NIDDM. Diabetes 1989 38, 1314–1319.CrossRefGoogle ScholarPubMed
Cusi, K, Maezono, K, Osman, A, Pendergrass, M, Patti, ME, Pratipanawatr, T, DeFronzo, RA, Kahn, CR & Mandarino, LJInsulin resistance differentially affects the PI 3-kinase- and MAP kinasemediated signaling in human muscle. J Clin Invest 2000 105, 311–320.CrossRefGoogle ScholarPubMed
De Bodo, RC, Steele, R, Altszuler, N, Dunn, A & Bishop, JSOn the hormonal regulation of carbohydrate metabolism: studies with 14C glucose. Recent Prog Horm Res 1963 19, 445–448.Google Scholar
Delarue, J, Normand, S, Pachiaudi, C, Beylot, M, Lamisse, F & Riou, JPThe contribution of naturally labelled 13C fructose to glucose appearance in humans. Diabetologia 1993 36, 338–345.CrossRefGoogle ScholarPubMed
Delarue, J, Maingourd, C, Lamisse, F, Garrigue, MA, Bagros, P & Couet, CGlucose oxidation after a peritoneal and an oral glucose load in dialyzed patients. Kidney Int 1994 45, 1147–1152.CrossRefGoogle Scholar
Delarue, J, Couet, C, Cohen, R, Brechot, JF, Antoine, JM & Lamisse, FEffects of fish oil on metabolic responses to oral fructose and glucose loads in healthy humans. Am J Physiol Endocrinol Metab 1996 270, E353–E362.CrossRefGoogle ScholarPubMed
Delarue, J, LeFoll, C, Corporeau, C & Lucas, DN-3 long chain polyunsaturated fatty acids: a nutritional tool to prevent insulin resistance associated to type 2 diabetes and obesity?” Reprod Nutr Dev 2004 44, 289–299.CrossRefGoogle ScholarPubMed
Dimitriadis, G, Leighton, B, Parry-Billings, M, Sasson, S, Young, M, Krause, U, Bevan, S, Piva, T, Wegener, G & Newsholme, EAEffects of glucocorticoid excess on the sensitivity of glucose transport and metabolism to insulin in rat skeletal muscle. Biochem J 1997 321, 707–712.CrossRefGoogle ScholarPubMed
Endres, S, Ghorbani, R, Kelley, VE, et al. The effect of dietary supplementation with n-3 polyunsaturated fatty acids on the synthesis of interleukin-1 and tumor necrosis factor by mononuclear cells. N Engl J Med 1989 320, 265–271CrossRefGoogle ScholarPubMed
Hawk, PBKjeldahl method. In Practical Physiological Chemistry, pp. 814–822. Toronto: Blakinston. 1977Google Scholar
Kahn, SEThe relative contributions of insulin resistance and beta-cell dysfunction to the pathophysiology of type 2 diabetes. Diabetologia 2003 46, 3–19.CrossRefGoogle Scholar
Livesey, G & Elia, MEstimation of energy expenditure, net carbohydrate utilization, and net fat oxidation and synthesis by indirect calorimetry: evaluation of errors with special reference to the detailed composition of fuels. Am J Clin Nutr 1988 47, 608–628.CrossRefGoogle Scholar
Montori, VM, Farmer, A, Wollan, PC & Dinneen, SFFish oil supplementation in type 2 diabetes: a quantitative systematic review Diabetes Care 2000 23, 1407–1415.CrossRefGoogle ScholarPubMed
Nicod, N, Giusti, V, Besse, C & Tappy, LMetabolic adaptations to dexamethasone-induced insulin resistance in healthy volunteers. Obes Res 2003 11, 625–631.CrossRefGoogle ScholarPubMed
Podolin, DA, Gayles, EC, Wei, Y, Thresher, JS & Pagliassotti, MJMenhaden oil prevents but does not reverse sucrose-induced insulin resistance in rats. Am J Physiol Endocrinol Metab 1998 274, R840–R848.Google Scholar
Proietto, J, Rohner-Jeanrenaud, F, Ionescu, E, Terrettaz, J, Sauter, JF & Jeanrenaud, BNon-steady-state measurement of glucose1 turnover in rats by using a one-compartment model. Am J Physiol Endocrinol Metab 1987 252, E77–E84.CrossRefGoogle Scholar
Puhakainen, I, Ahola, I & Yki-Jarvinen, HDietary supplementation with n-3 fatty acids increases gluconeogenesis from glycerol but not hepatic glucose production in patients with non-insulindependent diabetes mellitus Am J Clin Nutr 1995 61, 121–126.CrossRefGoogle ScholarPubMed
Rivellese, AA, Maffettone, A, Iovine, C, Di Marino, L, Annuzzi, G, Mancini, M & Riccardi, GLong-term effects of fish oil on insulin resistance and plasma lipoproteins in NIDDM patients with hypertriglyceridemia. Diabetes Care 1996 19, 1207–1213.CrossRefGoogle ScholarPubMed
Ryder, JW, Yang, J, Galuska, D, et al. Use of a novel impermeable biotinylated photolabeling reagent to assess insulin- and hypoxia-stimulated cell surface GLUT4 content in skeletal muscle from type 2 diabetic patients. Diabetes 2000 49, 647–654.CrossRefGoogle ScholarPubMed
Saad, MJ, Folli, F, Kahn, JA & Kahn, CRModulation of insulin receptor, insulin receptor substrate-1, and phosphatidylinositol 3- kinase in liver and muscle of dexamethasone-treated rats. J Clin Invest 1993 92, 2065–2072.CrossRefGoogle ScholarPubMed
Schneiter, P & Tappy, LKinetics of dexamethasone-induced alterations of glucose metabolism in healthy humans. Am J Physiol Endocrinol Metab 1998 275, E806–E813. CrossRefGoogle ScholarPubMed
Steele, R, Wall, JS, De Bodo, RC& Altszuler, N 1956 Measurement of size and turn over rate of body glucose pool by the isotope dilution method. Am J Physiol Endocrinol Metab 187, E15–E24.Google Scholar
Storlien, LH, Kraegen, EW, Chisholm, DJ, Ford, GL, Bruce, DG & Pascoe, WSFish oil prevents insulin resistance induced by high fat feeding in rats. Science 1987 237, 885 – 888.CrossRefGoogle ScholarPubMed
Storlien, LH, Jenkins, AB, Chisholm, DJ, Pascoe, WS, Khouri, S & Kraegen, EWInfluence of dietary fat composition on development of insulin resistance in rats. Diabetes 1991 40, 280–289.CrossRefGoogle ScholarPubMed
Taouis, M, Dagou, C, Ster, C, Durand, G, Pinault, M & Delarue, JN-3 polyunsaturated fatty acids prevent the defect of insulin receptor signaling in muscle. Am J Physiol Endocrinol Metab 2002 282, E664–E671.CrossRefGoogle ScholarPubMed
Tappy, L, Randin, D, Vollenweider, P, Vollenweider, L, Paquot, N, Scherrer, U, Schneiter, P, Nicod, P & Jequier, EMechanisms of dexamethasone-induced insulin resistance in healthy humans. J Clin Endocrinol Metab 1994 79, 1063 – 1069.Google ScholarPubMed
Tissot, S, Normand, S, Guilluy, R, Pachiaudi, C, Beylot, M, Laville, M, Cohen, R, Mornex, R & Riou, JPUse of a new gas chromatograph isotope ratio mass spectrometer to trace exogenous 13C labelled glucose at a very low level of enrichment in man. Diabetologia 1990 33, 449–456.CrossRefGoogle Scholar
Wajngot, A, Giacca, A, Grill, V, Vranic, M & Efendic, SThe diabetogenic effects of glucocorticoids are more pronounced in lowthan in high-insulin responders. Proc Natl Acad Sci U S A 1992 89, 6035–6039.CrossRefGoogle Scholar
Weinstein, SP, Wilson, CM, Pritsker, A & Cushman, SWDexamethasone inhibits insulin-stimulated recruitment of GLUT4 to the cell surface in rat skeletal muscle. Metabolism 1998 47, 3–6.CrossRefGoogle Scholar
Willi, SM, Kennedy, A, Wallace, P, Ganaway, ERogers, NL & Garvey, WTTroglitazone antagonizes metabolic effects of glucocorticoids in humans: effects on glucose tolerance, insulin sensitivity, suppression of free fatty acids, and leptin. Diabetes 2002 51, 2895–2902.CrossRefGoogle ScholarPubMed