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The interaction between nutritional status and growth hormone in young cattle: differential responsiveness of fat and protein metabolism

Published online by Cambridge University Press:  09 March 2007

Janet M. Dawson ,
Henry M. R. Greathead ,
Jim Craigon ,
David L. Hachey ,
Peter J. Reeds ,
Jennifer M. Pell  and
Peter J. Buttery
Janet M. Dawson*
Affiliation:
School of Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
Henry M. R. Greathead
Affiliation:
School of Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
Jim Craigon
Affiliation:
School of Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
David L. Hachey
Affiliation:
USDA Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA
Peter J. Reeds
Affiliation:
USDA Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA
Jennifer M. Pell
Affiliation:
Babraham Institute, Babraham, Cambridge CB2 4AT, UK
Peter J. Buttery
Affiliation:
School of Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
*
*Corresponding author:Dr Janet Dawson, fax +44 (0) 115 951 6122, email [email protected]
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Abstract

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The effect of dietary intake level on in vivo plasma leucine and plasma palmitate flux rates and on the response to a bolus injection of bovine growth hormone (GH) was investigated in six young steers. Animals were fed on a pelleted diet of dried grass–barley (0.7:0.3, w/w) in quantities sufficient to supply 0.8, 1.2, 1.6, 2.0, 2.4 or 2.65 × maintenance energy requirement, offered in hourly portions. Continuous intravenous infusions of [1-13C]leucine or [1-13C]palmi-tate were used to determine the flux of amino acid and fatty acid through the plasma pool before, immediately (1–3 h) after and 22–24 h after a subcutaneous injection of bovine GH (0.55 mg/kg body weight). Hourly blood samples were taken for 27 h to monitor the temporal responses of circulating hormones and metabolites following GH administration. The animal on the lowest plane of nutrition had elevated plasma GH and reduced insulin-like growth factor-1 concentrations compared with those fed on higher intake levels. Plasma leucine flux and leucine concentration increased with intake while palmitate flux and plasma non-esterified fatty acid (NEFA) concentrations were inversely related to intake. Leucine flux rate decreased in the animals fed on the two highest intake levels in response to GH 22–24 h after administration, but plasma leucine concentrations were reduced in all animals at this time. Only the animal fed on the lowest intake level showed an immediate response to GH (within 3 h of administration) with increased palmitate flux and plasma NEFA concentrations but a lipolytic response was apparent in other animals 22–24 h post-administration although the magnitude of the response was markedly reduced at high intakes. We conclude that lipid and protein metabolism are differentially responsive to GH and nutritional status.

Keywords

Growth hormonePalmitateLeucineCattle
Type
Animal Nutrition
Information
British Journal of Nutrition , Volume 79 , Issue 3 , March 1998 , pp. 275 - 286
Copyright
Copyright © The Nutrition Society 1998

References

Agricultural and, Food Research Council (1993) Energy and Protein Requirements of Ruminants. Wallingford: CAB INTERNATIONAL.Google Scholar
Bass, JJ, Spencer, GSG & Hodgkinson, SC (1992) Nutritional control of the growth hormone axis. In The Control of Fat and Lean Deposition pp. 175–195 [Buttery, Boorman, PJ KN, Lindsay, DB, editors]. Oxford: Butterworth-Heinemann.CrossRefGoogle Scholar
Bauman, DE, Peel, CJ, Steinhour, WD, Reynolds, PJ, Tyrrell, HF, Brown, ACG & Haaland, GL (1988) Effect of bovine somatotropin on metabolism of lactating dairy cows: influence on rates of irreversible loss and oxidation of glucose and nonesterified fatty acids. Journal of Nutrition 118, 1031–1040.CrossRefGoogle ScholarPubMed
Bauman, DE & Vernon, RG (1993) Effects of exogenous bovine somatotropin on lactation. Annual Review of Nutrition 13, 437–461.CrossRefGoogle ScholarPubMed
Berthold, HK, Hachey, DL, Reeds, PJ, Thomas, OP, Hoeksema, S & Klein, PD (1991) Uniformly 13C-labeled algal protein used to determine amino acid essentiality in vivo. Proceedings of the National Academy of Sciences USA 88, 8091–8095.CrossRefGoogle ScholarPubMed
Brameld, JM, Atkinson, JL, Saunders, JC, Pell, JM, Buttery, PJ & Gilmour, RS (1996) Effects of growth hormone administration and dietary protein intake on insulin-like growth factor-I (IGF-I) and growth hormone receptor (GHR) mRNA expression in porcine liver, skeletal muscle and adipose tissue. Journal of Animal Science 74, 1832–1841.CrossRefGoogle ScholarPubMed
Breier, BH, Bass, JJ, Butler, JH & Gluckman, PD (1986) The somatotrophic axis in young steers: influence of nutritional status on pulsatile release of growth hormone and circulating concentrations of insulin-like growth factor-1. Journal of Endocrinology 111, 209–215.CrossRefGoogle ScholarPubMed
Breier, BH, Gluckman, PD & Bass, JJ (1988) Influence of nutritional status and oestradiol-17β on plasma growth hormone, insulin-like growth factors-I and -II and the response to exogenous growth hormone in young steers. Journal of Endocrinology 118, 243–250.CrossRefGoogle ScholarPubMed
Breier, BH & Sauerwein, H (1995) Regulation of growth in ruminants by the somatotropic axis. In Ruminant Physiology: Digestion, Metabolism, Growth and Reproduction, pp. 451–474 [Engelhardt, W von, Leonhard-Mareck, S, Breves, G, Giesecke, D, editors]. Stuttgart, Germany: Ferdinand Enke Verlag.Google Scholar
Coleman, ME, Russell, L & Etherton, TD (1994) Porcine somatotropin (pST) increases IGF-1 mRNA abundance in liver and subcutaneous adipose tissue but not in skeletal muscle of growing pigs. Journal of Animal Science 72, 918–924.CrossRefGoogle Scholar
Crompton, LA & Lomax, MA (1993) Hindlimb protein turnover and muscle protein synthesis in lambs: a comparison of techniques. British Journal of Nutrition 69, 345–358.CrossRefGoogle ScholarPubMed
Dawson, JM, Essex, CP, Walsh, A, Beever, DE, Gill, M & Buttery, PJ (1993 a) Effect of fishmeal supplementation and β-agonist administration on adipose tissue metabolism in steers given silage. Animal Production 57, 397–406.Google Scholar
Dawson, JM, Greathead, HMR, Hachey, DL, Reeds, PJ, Buttery, PJ, Pell, JM & Beever, DE (1993 b) The interaction between plane of nutrition and response to growth hormone on fat and protein metabolism in cattle. Proceedings of the Nutrition Society 52, 301A.Google Scholar
Digby, P, Galway, N & Lane, P (1989) Genstat 5, A Second Course. Oxford: Oxford Science Publications.Google Scholar
Douglas, RG, Gluckman, PD, Ball, K, Breier, BH & Shaw, JHF (1991) The effects of infusion of insulin-like growth factor (IGF)-I, IGF-II and insulin on glucose and protein metabolism in fasted lambs. Journal of Clinical Investigation 88, 614–622.CrossRefGoogle ScholarPubMed
Dunshea, FR, Bauman, DE, Boyd, RD & Bell, AW (1992) Temporal response of circulating metabolites and hormones during somatotropin treatment of growing pigs. Journal of Animal Science 70, 123–131.CrossRefGoogle ScholarPubMed
Eisemann, JH, Hammond, AC, Bauman, DE, Reynolds, PJ, McCutcheon, SN, Tyrrell, HF & Haaland, GL (1986) Effect of bovine growth hormone administration on metabolism of growing Hereford heifers: protein and lipid metabolism and plasma concentrations of metabolites and hormones. Journal of Nutrition 116, 2504–2515.CrossRefGoogle ScholarPubMed
Eisemann, JH, Hammond, AC, Rumsey, TS & Bauman, DE (1989) Nitrogen and protein metabolism and metabolites in plasma and urine of beef steers treated with somatotropin. Journal of Animal Science 67, 105–115.CrossRefGoogle ScholarPubMed
Elsasser, TH, Rumsey, TS & Hammond, AC (1989) Influence of diet on basal and growth hormone-stimulated plasma concentrations of IGF-1 in beef cattle. Journal of Animal Science 67, 128–141.CrossRefGoogle ScholarPubMed
Hachey, DL, Patterson, BW, Reeds, PJ & Elsas, LJ (1991) Isotopic determination of organic keto acid pentafluorobenzyl esters in biological fluids by negative chemical ionization gas chromato-graphy/mass spectrometry. Analytical Chemistry 63, 919–923.Google Scholar
Kriel, GV, Bryant, MJ & Lomax, MA (1992) Effect of dietary protein intake and intravenous glucose infusion on plasma concentrations of insulin-like growth factor-1 in lambs. Journal of Endocrinology 132, 195–199.CrossRefGoogle ScholarPubMed
Lobley, GE (1992) Control of the metabolic fate of amino acids in ruminants: a review. Journal of Animal Science 70, 3264–3275.CrossRefGoogle ScholarPubMed
McGuire, MA, Bauman, DE, Dwyer, DA & Cohick, WS (1995) Nutritional modulation of the somatotropin/insulin-like growth factor system: response to feed deprivation in lactating cows. Journal of Nutrition 125, 493–502.Google ScholarPubMed
McGuire, MA, Vicini, JL, Bauman, DE & Veenhuizen, JJ (1992) Insulin-like growth factors and binding proteins in ruminants and their nutritional regulation. Journal of Animal Science 70, 2901–2910.Google ScholarPubMed
McShane, TM, Schillo, KK, Estienne, MJ, Boling, JA, Bradley, NW & Hall, JB (1989) Effects of recombinant DNA-derived somatotropin and dietary energy intake on development of beef heifers: II. Concentrations of hormones and metabolites in blood sera. Journal of Animal Science 67, 2237–2244.CrossRefGoogle ScholarPubMed
Maiter, D, Underwood, LE, Maes, M & Ketelslegers, JM (1988) Acute down regulation of the somatogenic receptors in rat liver by a single injection of growth hormone. Endocrinology 122, 1291–1296.CrossRefGoogle ScholarPubMed
Mills, SE, Lemenager, RP & Hortsman, LA (1989) Adipose tissue lipogenesis in growing steers adapted to different levels of feed intake. Journal of Animal Science 67, 3011–3017.CrossRefGoogle ScholarPubMed
National Research Council (1994) Metabolic Modifiers: Effects on the Nutrient Requirements of Food-Producing Animals. Washington: National Academic Press.Google Scholar
Oddy, VH, Warren, HM, Moyse, KJ & Owens, PC (1991) IGF-1 inhibits muscle protein degradation in sheep. In 2nd International Symposium on Insulin-like Growth Factors/Somatomedins, p. 281 Abstr. San Francisco, CA: University of California.Google Scholar
Pell, JM & Bates, PC (1992) Differential actions of growth hormone and insulin-like growth factor-1 on tissue protein metabolism in dwarf mice. Endocrinology 130, 1942–1950.Google ScholarPubMed
Peters, JP (1986) Consequences of accelerated gain and growth hormone administration for lipid metabolism in growing beef steers. Journal of Nutrition 116, 2490–2503.CrossRefGoogle ScholarPubMed
Roe, JA, Harper, JMM & Buttery, PJ (1989) Protein metabolism in ovine primary muscle cultures derived from satellite cells – effects of selected peptide hormones and growth factors. Journal of Endocrinology 122, 565–571.CrossRefGoogle ScholarPubMed
Sauerwein, H, Breier, BH, Bass, JJ & Gluckman, PD (1991) Chronic treatment with bovine growth hormone up-regulates high-affinity hepatic somatotropic receptors in sheep. Acta Endocrinologica 12, 306–313.Google Scholar
Smith, SB, Prior, RL, Koong, LJ & Mersmann, HJ (1992) Nitrogen and lipid metabolism in heifers fed at increasing levels of intake. Journal of Animal Science 70, 152–160.CrossRefGoogle ScholarPubMed
Tesseraud, S, Grizard, J, Debras, E, Papet, I, Bonnet, Y, Bayle, G & Champredon, C (1993) Leucine metabolism in lactating and dry goats: effect of insulin and substrate availability. American Journal of Physiology 265, E402–E413.Google ScholarPubMed
Thissen, J-P, Triest, S, Moats-Staats, BM, Underwood, LE, Mauerhoff, T, Maiter, D & Ketelslegers, J-M (1991) Evidence that pretranslational and translational defects decrease serum insulin-like growth factor-1 concentrations during dietary protein restriction. Endocrinology 129, 429–433.CrossRefGoogle ScholarPubMed
Tindal, JS, Blake, LA, Simmonds, AD & Hart, IC (1985) Inhibition of growth hormone release by rumen distension in goats. Journal of Endocrinology 104, 159–163.CrossRefGoogle ScholarPubMed
Vernon, RG & Flint, DJ (1989) Role of growth hormone in the regulation of adipocyte growth and function. In Biotechnology in Growth Regulation, pp. 57–71 [ProsserRB Heap, CG RB Heap, CG, Lamming, GE, editors]. London: Butterworth.CrossRefGoogle Scholar
Winer, BJ, Brown, DR & Michels, KM (1991) Statistical Principles in Experimental Design, 3rd ed. New York: McGraw-Hill Inc.Google Scholar
Wolfe, RR (1992) Radioactive and Stable Isotope Tracers in Biomedicine: Principles and Practice of Kinetic Analysis. New York: Wiley-Liss.Google Scholar