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The intake of fried virgin olive or sunflower oils differentially induces oxidative stress in rat liver microsomes

Published online by Cambridge University Press:  09 March 2007

José L. Quiles ,
Jesús R. Huertas ,
Maurizio Battino ,
M. Carmen Ramírez-Tortosa ,
Modesta Cassinello ,
José Mataix ,
Magdalena Lopez-Frias  and
Mariano Mañas
José L. Quiles*
Affiliation:
Institute of Nutrition and Food Technology, Dept of Physiology, University of Granada, Granada, Spain
Jesús R. Huertas
Affiliation:
Institute of Nutrition and Food Technology, Dept of Physiology, University of Granada, Granada, Spain
Maurizio Battino
Affiliation:
Institute of Biochemistry, Faculty of Medicine, University of Ancona, Ancona, Italy
M. Carmen Ramírez-Tortosa
Affiliation:
Institute of Nutrition and Food Technology, Dept of Biochemistry and Molecular Biology, University of Granada, Granada, Spain
Modesta Cassinello
Affiliation:
Institute of Nutrition and Food Technology, Dept of Physiology, University of Granada, Granada, Spain
José Mataix
Affiliation:
Institute of Nutrition and Food Technology, Dept of Physiology, University of Granada, Granada, Spain
Magdalena Lopez-Frias
Affiliation:
Institute of Nutrition and Food Technology, Dept of Physiology, University of Granada, Granada, Spain
Mariano Mañas
Affiliation:
Institute of Nutrition and Food Technology, Dept of Physiology, University of Granada, Granada, Spain
*
*Corresponding author:Dr José L. Quiles, fax +34 958 248 326, email [email protected]
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Abstract

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The effects of non-fried and fried virgin olive and sunflower oils on rat liver microsomal compositional features have been investigated. In addition, plasma antioxidants (α-tocopherol and ubiquinone 9) were investigated as well as the possible oxidative modifications suffered by virgin olive and sunflower oils during the frying process. The frying process decreased the content of α-tocopherol and phenolics in the oils and increased total polar materials. Sunflower oil was affected to a greater extent than olive oil. In rats, the intake of fried oil led to higher levels of lipid peroxidation and a lower concentration of plasma antioxidants. Microsomal fatty acid and antioxidant profiles were also altered. It seems that a strong relationship exists between the loss of antioxidants and the production of toxic compounds in the oils after frying and the extent of the peroxidative events in microsomes, which were also different depending on the fat source. The highly unsaturated sunflower oil was less resistant to the oxidative stress produced by frying and led to a higher degree of lipid peroxidation in liver microsomes in vivo than virgin olive oil.

Keywords

Virgin olive oilSunflower oilFried oilLipid peroxidationRat
Type
Research Article
Information
British Journal of Nutrition , Volume 88 , Issue 1 , July 2002 , pp. 57 - 65
Copyright
Copyright © The Nutrition Society 2002

References

American Institute of Nutrition (1977) Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
Amiot, MT, Fleuriet, A & Macheix, JT (1986) Importance and evolution of phenolic compounds in olive during growth and maturation. Journal of Agricultural and Food Chemistry 34 823–826.CrossRefGoogle Scholar
Barrera-Arellano, D, Marquez-Ruiz, G & Dobarganes, MC (1997) A simple procedure to evaluate the performance of fats and oils at frying temperatures. Grasas y Aceites 48 231–235.CrossRefGoogle Scholar
Barzanti, V, Battino, M, Baracca, A, Cavazzoni, M, Cocchi, M, Noble, R, Maranesi, M, Turchetto, E & Lenaz, G (1994) The effect of dietary lipid changes on the fatty acid composition and function of liver, heart and brain mitochondria in the rat at different ages. British Journal of Nutrition 71, 193–202.CrossRefGoogle ScholarPubMed
Battino, M, Bullon, P, Wilson, M & Newman, H (1999) Oxidative injury and inflammatory periodontal diseases: the challenge of anti-oxidants to free radicals and reactive oxygen species. Critical Reviews in Oral Biology and Medicine 10, 458–476.CrossRefGoogle ScholarPubMed
Charnock, JS, McLennan, PL & Abeywardena, MY (1992) Dietary modulation of lipid metabolism and mechanical performance of the heart. Molecular and Cellular Biochemistry 116, 19–25.CrossRefGoogle ScholarPubMed
Cinquanta, L, Esti, M & La Notte, E (1997) Evolution of phenolic compounds in virgin olive oil during storage. Journal of the American Oil Chemists' Society 74, 1259–1264.CrossRefGoogle Scholar
Cortesi, N, Azzolini, M, Rovellini, P & Fedeli, E (1995) I componenti minori polari degli oli vergini di oliva: ipotesi di structuttura mediante LC-MS (Minor polar components from virgin olive oil: hypothesis of structure by LC-MS). Rivista Italiana di Sostanze Grasse 43, 1802–1807.Google Scholar
Dobarganes, MC, Pérez, MC & Gutiérrez, R (1984) Métodos analíticos de aplicación en grasas calentadas. I. Determinación de ésteres metílicos no alterados (Analytical methods for application on heated fats. I. Determination of non modified methyl esters). Grasas y Aceites 35, 172–177.Google Scholar
Donelly, JK & Robinson, DS (1995) Free radicals in foods. Free Radical Research 22, 147–176.CrossRefGoogle Scholar
European Economic Community (1991) On the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis. Diario Oficial de las Communidades Europeas L248 1–82.Google Scholar
Fillion, L & Henry, CJ (1998) Nutrient losses and gains during frying: a review. International Journal of Food Sciences and Nutrition 49, 157–168.CrossRefGoogle ScholarPubMed
Fleischer, S, McIntyre, IO & Vidal, JC (1979) Large scale preparation of rat liver mitochondria in high yield. Methods in Enzymology 55, 32–39.CrossRefGoogle ScholarPubMed
Grootveld, M, Atherton, MD, Sheerin, AN, Haweks, J, Blake, DR, Richens, TE, Silwood, CJL, Lynch, E & Claxon, AWD (1998) In vivo absorption, metabolism, and urinary excretion of alpha, beta-unsaturated aldehydes in experimental animals. Relevance to the development of cardiovascular diseases by the dietary ingestion of thermally stressed polyunsaturate-rich culinary oils. Journal of Clinical Investigation 101, 1210–1218.CrossRefGoogle Scholar
Gunstone, FD, Harwood, JL & Padley, FB (1994) The Lipid Handbook, 2nd ed., pp. 53–117. London: Chapman & Hall.Google Scholar
Gutfinger, T (1981) Polyphenols in olive oils. Journal of the American Oil Chemists' Society 58, 966–968.CrossRefGoogle Scholar
Halliwell, B & Gutteridge, JMC (1999) Free Radicals in Biology and Medicine. Oxford: Oxford University Press.Google Scholar
Huertas, JR, Battino, M, Lenaz, G & Mataix, J (1991 a) Changes in mitochondrial and microsomal rat liver coenzyme Q9 and Q10 content induced by dietary fat and endogenous lipid peroxidation. FEBS Letters 287, 89–92.CrossRefGoogle ScholarPubMed
Huertas, JR, Battino, M, Mataix, FJ & Lenaz, G (1991 b) Cytochrome oxidase induction after oxidative stress induced by adriamycin in liver of rats fed with dietary olive oil. Biochemical and Biophysical Research Communications 181, 375–382.CrossRefGoogle ScholarPubMed
Lang, JK & Packer, L (1987) Quantitative determination of vitamin E and oxidised and reduced CoQ by high-performance liquid chromatography with in-line ultraviolet and electrochemical detection. Journal of Chromatography 385, 109–117.CrossRefGoogle Scholar
Lepage, G & Roy, CC (1986) Direct transesterification of all classes of lipids in one-step reaction. Journal of Lipid Research 27, 114–120.CrossRefGoogle ScholarPubMed
Liu, JF & Huang, CJ (1995) Tissue alpha-tocopherol retention in male rats is compromised by feeding diets containing oxidized frying oil. Journal of Nutrition 125, 3071–3080.Google ScholarPubMed
Liu, JF & Huang, CJ (1996) Dietary oxidized frying oil enhances tissue alpha-tocopherol depletion and radioisotope tracer excretion in vitamin E-deficient rats. Journal of Nutrition 126, 2227–2235.CrossRefGoogle ScholarPubMed
Lowry, OH, Rosebrough, NJ, Farr, AL & Randall, RG (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
MacCrehan, WA (1990) Determination of retinol, α-tocopherol and β-carotene in serum by liquid chromatography. Methods in Enzymology 189, 172–181.CrossRefGoogle ScholarPubMed
McMillin, JB, Bick, RJ & Benedict, CR (1992) Influence of dietary fish oil on mitochondrial function and response to ischemia. American Journal of Physiology 263, H1479–H1485.Google ScholarPubMed
Mataix, J, Quiles, JL, Huertas, JR, Battino, M & Mañas, M (1998) Tissue specific interactions of exercise, dietary fatty acids, and vitamin E in lipid peroxidation. Free Radicals in Biology and Medicine 24, 511–521.CrossRefGoogle ScholarPubMed
Melton, SL, Jafar, S, Sykes, D & Trigiano, MK (1994) Review of stability measurements for frying oils and fried food flavor. Journal of the American Oil Chemists' Society 71, 1301–1308.CrossRefGoogle Scholar
Nourooz-Zadeh, J, Tajaddini-Sarmadi, J & Wolff, S (1994) Measurement of plasma hydroperoxide concentrations by the ferrous oxidation–xylenol orange assay in conjunction with triphenylphosphine. Analytical Biochemistry 220, 403–409.CrossRefGoogle ScholarPubMed
Orrenius, S, Moldeus, P, Theor, H & Hogberg, J (1977) Microsomes and Drugs Oxidations. New York, NY: Pergamon.Google Scholar
Paul, S & Mittal, GS (1997) Regulating the use of degraded oil/fat in deep-fat/oil food frying. Critical Reviews in Food Science and Nutrition 37, 635–662.CrossRefGoogle ScholarPubMed
Quiles, JL, Huertas, JR, Mañas, M, Battino, M & Mataix, J (1999a) Physical exercise affects the lipid profile of mitochondrial membranes in rats fed with virgin olive oil or sunflower oil. British Journal of Nutrition 81, 21–24.CrossRefGoogle ScholarPubMed
Quiles, JL, Huertas, JR, Mañas, M, Ochoa, JJ, Battino, M & Mataix, J (1999 b) Oxidative stress induced by exercise and dietary fat modulates the coenzyme Q and vitamin A balance between plasma and mitochondria. International Journal for Vitamin and Nutrition Research 69, 243–249.CrossRefGoogle ScholarPubMed
Quiles, JL, Ramírez-Tortosa, MC, Ibañez, S, González, A, Duthie, GG, Huertas, JR & Mataix, J (1999 c) Vitamin E supplementation increases the stability and the in vivo antioxidant capacity of refined olive oil. Free Radicals Research 31, 129–135.CrossRefGoogle ScholarPubMed
Ramírez-Tortosa, MC, Aguilera, CM, Quiles, JL & Gil, A (1998) Influence of dietary lipids on lipoprotein composition and LDL Cu2+ induced oxidation in rabbits with experimental atherosclerosis. Biofactors 8, 79–85.CrossRefGoogle ScholarPubMed
Ramírez-Tortosa, MC, Gil, A, Quiles, JL & Mataix, J (1997) Rabbit liver mitochondria coenzyme Q10 and hydroperoxide levels: an experimental model of atherosclerosis. Molecular Aspects of Medicine 18, 233–236.CrossRefGoogle ScholarPubMed
Ramírez-Tortosa, C, Lopez-Pedrosa, JM, Suarez, A, Ros, E, Mataix, J & Gil, A (1999) Olive oil- and fish oil-enriched diets modify plasma lipids and susceptibility of LDL to oxidative modification in free-living male patients with peripheral vascular disease: the Spanish Nutrition Study. British Journal of Nutrition 82, 31–39.CrossRefGoogle ScholarPubMed
Stillwell, W, Jenski, LJ, Crump, FT & Ehringer, W (1997) Effect of docosahexaenoic acid on mouse mitochondrial membrane properties. Lipids 32, 497–506.Google ScholarPubMed
Svegliati-Baroni, S, Amelio, M, Sangiorgi, Z, Gaddi, A & Battino, M (1999) Solid monounsaturated diet lowers LDL unsaturation trait and oxidisability in hypercholesterolemic (type IIb) patients. Free Radical Research 30, 275–285.CrossRefGoogle Scholar
Takeoka, GR, Fuli, GH & Dao, LT (1997) Effect of heating on the characteristics and chemical composition of selected frying oils and fats. Journal of Agricultural and Food Chemistry 45, 3244–3249.CrossRefGoogle Scholar
Tsimidou, M, Lytridou, M, Boskou, D, Pappa-Louisi, A, Kotsifaki, F & Petrakis, C (1996) On the determination of minor phenolic acids of virgin olive oil by RP-HPLC. Grasas y Aceites 47, 151–157.CrossRefGoogle Scholar
Ueda, T & Igarashi, O (1990) Determination of vitamin E in biological specimens and foods by HPLC. Pretreatment of Samples and extraction of tocopherols. Journal of Micronutrient Analysis 7, 79–96.Google Scholar
Vázquez-Roncero, A, Janer del Valle, C & Janer del Valle, ML (1973) Determinación de los polifenoles totales del aceite de oliva. Grasas y Aceites 24, 350–355.Google Scholar
Warner, K (1999) Impact of high-temperature food processing on fats and oils. Advances in Experimental Medicine and Biology 459, 67–77.CrossRefGoogle ScholarPubMed
Yamaoka, S, Urade, R & Kito, M (1988) Mitochondrial function in rats is affected by modification of membrane phospholipids with dietary sardine oil. Journal of Nutrition 118, 290–296.CrossRefGoogle ScholarPubMed