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Intake, digestion and small intestinal protein availability in sheep in relation to ammoniation of wheat straw with or without protein supplementation

Published online by Cambridge University Press:  09 March 2007

S. J. Oosting ,
J. Van Bruchem  and
X. B. Chen
S. J. Oosting
Affiliation:
Department of Animal Husbandry, Section of Tropical Animal Production, Agricultural University, PO Box 338, NL 6700, AH Wageningen, The Netherlands
J. Van Bruchem
Affiliation:
Department of Animal and Human Physiology, Agricultural University, Haarweg 10, NL 6709 PJ Wageningen, The Netherlands
X. B. Chen
Affiliation:
The Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB2 9SB
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Abstract

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The effects of ammoniation of wheat straw with or without supplementation of protein sources of either high (casein) or relatively low (potato protein) rumen degradability on intake and digestion were studied with four sheep in a 4 × 4 Latin square design. Rations offered were: (1) untreated wheat straw (UWS), (2) ammoniated wheat straw (AWS), (3) AWS supplemented with 3·2 g casein/kg live weight (W)0·75 per d (AWSC) and (4) AWS supplemented with 3·9 g potato protein/kg W0·75per d (AWSP). Straw was offered ad lib. and all rations were supplemented with sugarbeet pulp and a mineral mixture. NH3 treatment increased intake and digestion. Supplementation of AWS with potato protein increased total digestible organic matter intake (DOMI) compared with AWS whereas supplementation with casein did not affect total DOMI. Protein supplementation of AWS significantly reduced rumen digestion of cellulose, and when the supplementation was with casein it reduced rumen digestion of neutral-detergent fibre and hemicellulose also. This lower rumen digestion was compensated by a higher proportion of digestion occurring in the hindgut for hemicellulose (P<0·05 for AWSC, P>0·05 for AWSP), but not for cellulose. Across all rations, rumen fluid volume increased with increasing cell-wall intake. The efficiencies of microbial protein synthesis were (average of three different methods of estimation) 23·3, 26·2, 34·8 and 31·7gN/kg apparently-rumen-degraded organic matter for UWS, AWS, AWSC and AWSP respectively. The difference between UWS and AWS was not significant, but values for AWSC and AWSP were significantly higher than that for AWS. The rumen digestion of feed aimno acid-N (AA-N) was significantly higher for AWSC than for the other rations. The apparent small-intestinal digestion of AA-N and N was significantly higher for AWSP than for the other rations. The true small-intestinal digestion values were 0·86, 0·84 and 0·68 for AA-N, N and non-protein-N respectively. Heal endogenous losses of AA-N were approximately 6 mg/g duodenal non-protein dry-matter flow. Linear relationships were observed between DOMI and N balance and truly absorbed AA-N, indicating that DOMI could have been limited by small-intestinal amino acid availability. Regression of N balance v. truly absorbed AA-N resulted in an estimate of net efficiency of utilization of truly absorbed AA-N of 0·54.

Keywords

Protein availabilityAmmoniated wheat strawN balanceMicrobial protein synthesis
Type
Digestion of ammoniated wheat straw
Information
British Journal of Nutrition , Volume 74 , Issue 3 , September 1995 , pp. 347 - 368
Copyright
Copyright © The Nutrition Society 1995

References

Agricultural Research Council (1980) The Nutrient Requirements of Ruminant Livestock. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Barnes, R. J. (1980) Near infra-red spectra of ammonia-treated straw and of isolated cell walls. Animal Feed Science and Technology 21, 209–218.CrossRefGoogle Scholar
Brouwer, B. O. (1989) DBSTAT User's Guide. Wageningen, The Netherlands: Department of Animal Husbandry, Wageningen Agricultural University.Google Scholar
Chen, X. B., Hovell, F. D. DeB, Ørskov, E. R. & Brown, D. S. (1990 a) Excretion of purine derivatives by ruminants: effect of endogenous nucleic acid supply on purine derivative excretion by sheep. British Journal of Nutrition 63, 131–142.CrossRefGoogle ScholarPubMed
Chen, X. B., Mathieson, J., Hovell, F. D. DeB. & Reeds, P. J. (1990 b) Measurement of purine derivatives in urine of ruminants using automated methods. Journal of the Science of Food and Agriculture 53, 23–33.CrossRefGoogle Scholar
Chen, X. B., Ørskov, E. R. & Hovell, F. D. DeB. (1991) The use of intragastric infusion in studies on excretion of purine derivatives as a measure of protein supply in ruminants. In Protein Metabolism and Nutrition, European Association of Animal Production Publication no. 59, Vol. 2, short communications, pp. 67–70 [Eggum, B.O., Boisen, S., Børsting, C., Danfaer, A. and Hvelplund, T., editors]. Foulum, Denmark: National Institute of Animal Science.Google Scholar
Demeyer, D. I. (1991) Quantitative aspects of microbial metabolism in the rumen and hindgut. In Rumen Microbial Metabolism and Ruminant Digestion, pp. 217–239 [Jouany, J. P., editor]. Paris: INRA.Google Scholar
Doyle, P. T. & McLaren, C. E. (1988) Utilization of clover diets by sheep. II. Intake, digestion and utilisation of nitrogen and sulfur. Australian Journal of Agricultural Research 39, 881–890.CrossRefGoogle Scholar
Doyle, P. T. & Panday, S. B. (1990) The feeding value of cereal straws for sheep. III. Supplementation with minerals or minerals and urea. Animal Feed Science and Technology 29, 29–43.CrossRefGoogle Scholar
Egan, A. R. (1965) Nutritional status and intake regulation in sheep. III. The relationship between improvement of nitrogen status and increase in voluntary intake of low-protein roughages by sheep. Australian Journal of Agricultural Research 16, 463–472.CrossRefGoogle Scholar
Egan, A. R. (1977) Nutritional status and intake regulation in sheep. VII. Relationships between the voluntary intake of herbage by sheep and the protein/energy ratio in the digestion products. Australian Journal of Agricultural Research 28, 907–915.CrossRefGoogle Scholar
Elliott, R. C. & Topps, J. H. (1964) Effects of various low protein diets on the distribution of ruminal nitrogen and on the nitrogen required for maintenance of African sheep. Animal Production 6, 345–355.Google Scholar
Faichney, G. J. (1980) The use of markers to measure digesta flow from the stomach of sheep fed once daily. Journal of Agricultural Science, Cambridge 94, 313–319.CrossRefGoogle Scholar
Grenet, E. & Demarquilly, C. (1977) Utilisation de l'azote des fourrages vertes par le mouton en croissance: influence du stade de végétation, de l'espéce fourragère, de la fertilisation azotée et de l'addition d'orge (utilization of fresh forage by growing sheep: influence of stage of maturity, forage type, nitrogen fertilization and barley supplementation). Annales de Zootechnie 26, 481–501.CrossRefGoogle Scholar
Grovum, W. L. & Williams, V. J. (1973) Rate of passage of digesta in sheep. 4. Passage of marker through the alimentary tract and the biological relevance of rate constants from the changes in concentration of marker in faeces. British Journal of Nutrition 30, 313–329.CrossRefGoogle ScholarPubMed
Hespell, R. B. & Bryant, M. P. (1979) Efficiency of rumen microbial growth: influence of some theoretical and experimental factors on YATP. Journal of Animal Science 49, 1640–1659.CrossRefGoogle ScholarPubMed
Hoover, W. H. (1986) Chemical factors involved in ruminal fiber digestion. Journal of Dairy Science 69, 2755–2766.CrossRefGoogle ScholarPubMed
Hvelplund, T. (1989) Protein evaluation of treated straws. In Evaluation of Straws in Ruminant Feeding, pp. 66–74 [Chenost, M. and Reiniger, P., editors]. Barking, Essex: Elsevier Science Publishers Ltd.Google Scholar
Kellaway, R. C. & Leibholz, J. (1983) Effects of nitrogen supplements on intake and utilization of low-quality forages. World Animal Review 48, 33–37.Google Scholar
Ketelaars, J. J. M. H. & Tolkamp, B. J. (1991) Toward a new theory of feed intake regulation. PhD Thesis, Agricultural University Wageningen, The Netherlands.Google Scholar
MacRae, J. C. & Lobley, G. E. (1982) Some factors which influence thermal energy losses during the metabolism of ruminants. Livestock Production Science 11, 447–456.CrossRefGoogle Scholar
MacRae, J. C., Smith, J. S., Dewey, P. J. S., Brewer, A. C., Brown, D. S. & Walker, A. (1985) The efficiency of utilization of metabolizable energy and apparent absorption of amino acids in sheep given spring-and autumn-harvested dry grass. British Journal of Nutrition 54, 197–209.CrossRefGoogle Scholar
Oosting, S. J., Boekholt, H. A, Los, M. J. M. & Leffering, C. P. (1993 a) Intake and utilization of energy from ammonia-treated and untreated wheat straw by steers and wether sheep fed a basal diet of grass pellets and hay. Animal Production 57, 227–236.Google Scholar
Oosting, S. J., Viets, T. C., Lammers-Wienhoven, S. C. W. & van Bruchem, J. (1993 b) Ammonia treatment of wheat straw. 2. Efficiency of microbial protein synthesis, rumen microbial protein pool size and turnover, and small intestinal protein digestion in sheep. Netherlands Journal of Agricultural Science 41, 135–151.CrossRefGoogle Scholar
Oosting, S. J., Vlemmix, P. J. M. & van Bruchem, J. (1994) Effect of ammonia treatment of wheat straw with or without supplementation of potato protein on intake, digestion and kinetics of comminution, rumen degradation and passage in steers. British Journal of Nutrition 72, 147–165.CrossRefGoogle ScholarPubMed
Øskov, E. R. (1982) Protein Nutrition in Ruminants. London: Academic Press Inc. Ltd., Harcourt Brace Jovanovich Publishers.Google Scholar
Owens, F. N. & Goetsch, A. L. (1986) Digesta passage and microbial protein synthesis. In Control of Digestion and Metabolism in Ruminants, pp. 196–227 [Milligan, L.P., Grovum, W. L. and Dobson, A., editors]. Englewood Cliffs, NJ: Prentice Hall.Google Scholar
Satter, L. D. & Slyter, L. L. (1974) Effect of ammonia concentration on rumen microbial protein production in vitro. British Journal of Nutrition 32, 199–208. Siddons, R. C., Beever, D. E. & Nolan, J. V. (1982). A comparison of methods for the estimation of microbial nitrogen in duodenal digesta of sheep. British Journal of Nutrition 48, 377–389.Google Scholar
Snedecor, G. W. & Cochran, W. G. (1967) Statistical Methods. Ames, USA: The lowa State University Press. Storm, E., Brown, D. S. & Ørskov, E. R. (1983). The nutritive value of rumen micro-organisms in ruminants. 3. The digestion of microbial amino and nucleic acids in, and losses from, the small intestine of sheep. British Journal of Nutrition 50, 479–485.Google Scholar
Tolkamp, B. J. & Ketelaars, J. J. M. H. (1992) Toward a new theory of feed intake regulation in ruminants. 2. Costs and benefits of feed consumption: an optimization approach. Livestock Production Science 30, 297–317.CrossRefGoogle Scholar
Tolkamp, B. J. & Ketelaars, J. J. M. H. (1993) The effect of ad lib feeding on the efficiency of energy utilization in growing and lactating cattle. Animal Production 56, 431–432.Google Scholar
Udén, P., Colluci, P. E. & van Soest, P. J. (1980) Investigation of chromium, cerium and cobalt as markers in digesta. Journal of the Science of Food and Agriculture 31, 625–632.CrossRefGoogle ScholarPubMed
Van Bruchem, J., Bongers, L. J. G. M., Lammers-Weinhoven, S. C. W., Bangma, G. A. & van Adrichem, P. W. M. (1989) Apparent and true digestibility of protein and amino acids in the small intestine of sheep as related to the duodenal passage of protein and non-protein dry matter. Livestock Production Science 23, 317–327.CrossRefGoogle Scholar
Van Bruchem, J., Oosting, S. J., Lammers-Wienhoven, S. C. W. & Leffering, C. P. (1993) Ammonia treatment of wheat straw. 1. Voluntary intake, chewing behaviour, rumen pool size and turnover and partition of digestion along the gastro-intestinal tract of sheep. Netherlands Journal of Agricultural Science 41, 111–133.CrossRefGoogle Scholar
Van Soest, P. J. (1982) Nutritional Ecology of the Ruminant. Corvallis, Oregon: O & B books.Google Scholar
Zorrilla-Rios, J., Horn, G. W. & McNew, R. W. (1991) Nutritive value of ammoniated wheat straw fed to cattle. Journal of Animal Science 69, 283–294.CrossRefGoogle ScholarPubMed