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Initial studies on leucine metabolism in the rumen of sheep

Published online by Cambridge University Press:  09 March 2007

M. Marsden ,
C. I. Bruce ,
C. G. Bartram  and
P. J. Buttery
M. Marsden
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD
C. I. Bruce
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD
C. G. Bartram
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD
P. J. Buttery
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD
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Abstract

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1. [3H]leucine infused directly into the rumen of sheep labelled microbial protein and, when compared with the specific activity of the rumen free-leucine pool, indicated that 50% of the bacterial protein leucine originated from the rumen free-leucine pool.

2. The lower limit for bacterial protein turnover in the rumen was 0.37/d when calculated as the difference between the specific rate of disappearances of labelled bacteria from the rumen and the liquid-phase dilution rate.

3. Intravenously infused leucine also labelled the rumen bacteria. The build-up of specific activity in the rumen bacteria was sigmoidal and did not resemble that of the salivary protein which suggested that the rumen epithelium was a major endogenous protein input to the rumen. Additionally, bacteria isolated from the rumen epithelium had high radioactivity indicating that they were ingesting the rumen epithelial cells.

Type
General Nutrition Papers
Information
British Journal of Nutrition , Volume 60 , Issue 1 , July 1988 , pp. 161 - 171
Copyright
Copyright © The Nutrition Society 1988

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