Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-08T12:27:43.082Z Has data issue: false hasContentIssue false
  • English
  • Français

Inhibitory effect of yoghurt and soya yoghurt containing bifidobacteria on the proliferation of Ehrlich ascites tumour cells in vitro and in vivo in a mouse tumour model

Published online by Cambridge University Press:  09 March 2007

I. A. Abd El-Gawad ,
E. M. El-Sayed ,
S. A. Hafez ,
H. M. El-Zeini  and
F. A. Saleh
I. A. Abd El-Gawad*
Affiliation:
Dairy Science and Technology Department, Faculty of Agriculture, Cairo University, PO 12613, Giza, Egypt
E. M. El-Sayed
Affiliation:
Dairy Science and Technology Department, Faculty of Agriculture, Cairo University, PO 12613, Giza, Egypt
S. A. Hafez
Affiliation:
Special Food and Technology Department, Food Technology Research Institute, Agriculture Research Center, Giza, Egypt
H. M. El-Zeini
Affiliation:
Dairy Science and Technology Department, Faculty of Agriculture, Cairo University, PO 12613, Giza, Egypt
F. A. Saleh
Affiliation:
Special Food and Technology Department, Food Technology Research Institute, Agriculture Research Center, Giza, Egypt
*
*Corresponding author: fax +20 2 7743824, Email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effect of yoghurt and soya yoghurt containing Bifidobacterium lactis Bb-12 or B. longum Bb-46 on Ehrlich ascites tumour cell proliferation was investigated in vitro and in vivo. Tumour cells were incubated with B. lactis Bb-12 or B. longum Bb-46 cultivated in de Mann Rogosa Sharpe (MRS) broth medium, or with their centrifuged supernatant fractions or sediments, for 2 h at 37°C. Treatment resulted in the inhibition of tumour cell proliferation by 85·42 (sd 0·78) and 85·10 (sd 1·28) % by intact micro-organisms, 77·61 (sd 0·29) and 71·43 (sd 1·75) % by their supernatant fractions, but only 4·00 (sd 0·19) and 9·09 (sd 1·24) % by the two sedimented bacteria, respectively. The incubation of tumour cells with yoghurt and soya yoghurt containing Bb-12 for 2 h resulted in 83·01 (sd 0·11) and 88·23 (sd 0·06) % inhibition, respectively, while it was 83·82 (sd 0·24) and 86·36 (sd 0·06) %, respectively for the same products containing Bb-46. Corresponding values for plain yoghurt and soya milk (without bifidobacteria) were 32·81 (sd 0·14) and 5·55 (sd 0·12) %, respectively. The differences between yoghurt or soya yoghurt containing Bb-12 or Bb-46 and plain yoghurt, soya milk or control treatments were statistically significant (n 3; P>0·05). Female Swiss albino mice were injected intraperitoneally with the same tumour cells. The lifespan of mice fed diets supplemented with yoghurt or soya yoghurt containing Bb-12 or Bb-46 was prolonged by 16, 23, 34 and 39%, respectively compared with that of the positive control group (n 6; P>0·05). The lifespan of groups fed plain yoghurt or soya milk was prolonged by 15 and 8%, respectively. Prolongation of lifespan was positively correlated with faeces bifidobacterial count in the groups fed yoghurt or soya yoghurt containing bifidobacteria (r 0·917; P>0·05).

Keywords

YoghurtSoya yoghurtEhrlich ascites tumour cellsBifidobacteria
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 1 , July 2004 , pp. 81 - 86
Copyright
Copyright © The Nutrition Society 2004

References

1Abdelali, H, Cassand, P, Soussotte, V, Daubeze, M, Bouley, C & Narbonne, JFEffect of dairy products on initiation of precursor lesions of colon cancer in rats. Nutr Cancer 1995 24, 121–132.Google Scholar
2Abd El-Gawad, IA, Hefny, AA, El-Sayed, EM & Saleh, FAReduction of flatulence-causing soymilk oligosaccharides by different starter cultures. In Proceedings of the 7th Egyptian Conference of Dairy Science and Technology, pp.125–144. [Abd El-Salam, MH, editor] Cairo, Egypt: ESDS-Publications. 1998.Google Scholar
3Anthony, MS, Clarkson, TB, Hughes, JRCL, Morgan, TM & Burke, GLSoybean isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal Rhesus monkeys. J Nutr 1996 126, 43–50.Google Scholar
4Baricault, L, Denariaz, G, Houri, JJ, Bouley, C,Sapin, C & Trugnan, GUse of HT-29, a cultured human colon cancer cell line, to study the effect of fermented milks on colon cancer cell growth and differentiation. Carcinogenesis 1995 16, 245–252.CrossRefGoogle Scholar
5Bennett, JM, Catousky, D, Danniel, MT, Galton, DAG, Greanlink, HR & Sultan, CProposal for the classification of the acute leukaemias. French-American-British (FAB) co-operative group. Br J Haematol 1976 33, 451–458.Google Scholar
6Biffi, A, Coradini, D, Larsen, R, Riva, L & Di Fronzo, GAntiproliferative effect of fermented milk on the growth of a human breast cancer cell line. Nutr Cancer 1997 28, 93–99.CrossRefGoogle ScholarPubMed
7Chen, RM, Wu, JJ, Lee, SC, Huang, AH & Wu, HMIncrease of intestinal Bifidobacterium and suppression of coliform bacteria with short-term yogurt ingestion. J Dairy Sci 1999 82, 2308–2314.Google Scholar
8Chou, CC & Hou, JWGrowth of bifidobacteria in soymilk and their survival in the fermented soymilk drink during storage. Int J Food Microbiol 2000 56, 113–121.Google Scholar
9De Simone, C, Bianchi Salvadori, B, Negri, R, Ferrazzi, M, Baldinelli, L & Vesely, RThe adjuvant effect of yogurt on production of gamma-interferon by ConA-stimulated human peripheral blood lymphocytes. Nutr Rep Int 1986 33, 419–433.Google Scholar
10Dinakar, P & Mistry, VVGrowth and viability of Bifidobacterium bifidum in cheddar cheese. J Dairy Sci 1994 77, 2854–2864.CrossRefGoogle ScholarPubMed
11El-Sayed, EM, Hefny, AA, Saleh, FA & Abd El-Gawad, IABifidobacteria as a starter for the manufacture of soy-yoghurt products. In Proceedings of the 7th Egyptian Conference of Dairy Science and Technology, pp 269–294. [Abd El-Salam, MH, editor] Cairo, Egypt: ESDS-Publications. 1998.Google Scholar
12Fernandes, CF & Shahani, KMAnticarcinogenic and immunological properties of dietary Lactobacilli. J Food Prot 1990 53, 704–710.CrossRefGoogle ScholarPubMed
13Friend, BA, Farmer, RE & Shahani, KMEffect of feeding and intraperitoneal implantation of yoghurt culture cells on Ehrlich ascites tumour. Milchwissenschaft 1982 37, 708–710.Google Scholar
14Hosono, A, Wardojo, R & Otani, HInhibitory effects of lactic acid bacteria from fermented milk on the mutagenicities of volatile nitrosamines. Agric Biol Chem 1990 54, 1639–1643.Google Scholar
15Kailasapathy, K & Rybka, SLactobacillus acidophilus and Bifidobacterium spp. - their therapeutic potential and survival in yoghurt. Austr J Dairy Technol 1997 52, 28–35.Google Scholar
16Kohwi, Y, Hashimoto, Y & Tamura, ZAntitumor and immunological adjuvant effect of Bifidobacterium infantis in mice. Bifid Microflora 1982 1, 61–66.Google Scholar
17Kohwi, Y, Imai, K, Tamura, Z & Hashimoto, YAntitumor effect of Bifidobacterium infantis in mice. Gann 1978 69, 613–618.Google Scholar
18Lee, SY, Vedemuthu, ER, Washam, CJ & Reinbold, BWAn agar medium for the differential enumeration of yoghurt starter bacteria. J Milk Food Tech 1973 37, 272.Google Scholar
19McCarthy, AC, La, E, Conti, CJ & Locniskar, MFEffect of spray-dried yogurt and lactic acid bacteria on the initiation and promotion stages of chemically induced skin carcinogenesis in mice. Nutr Cancer 1997 27, 231–237.Google Scholar
20Nagata, Y, Ishiwaki, N & Sugano, MStudies on the mechanism of the antihypercholesterolemic action of soy protein and soy protein type amino acid mixtures in relation to their casein counterparts in rats. J Nutr 1982 112, 1614–1625.Google Scholar
21Ohta, T, Nakatsugi, S, Watanabe, K, Kawamori, T, Ishikawa, F, Morotomi, M, Sugie, S, Toda, T, Sugimura, T & Wakabayashi, KInhibitory effects of Bifidobacterium -fermented soy milk on 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine-induced rat mammary carcinogenesis, with a partial contribution of its component isoflavones. Carcinogenesis 2000 21, 937–941.CrossRefGoogle ScholarPubMed
22Perdigon, G, Alvarez, S, Gobbato, N, de Budeguer, MV & de Ruiz Holgado, AAPComparative effect of the adjuvant capacity of Lactobacillus casei and lipopolysaccharide on the intestinal secretory antibody response and resistance to Salmonella infection in mice. Food Agric Immunol 1995 7, 283–294.CrossRefGoogle Scholar
23Perdigon, G, Valdez, JC & Rachid, MAntitumour activity of yoghurt: study of possible immune mechanisms. J Dairy Res 1998 65, 129–138.CrossRefGoogle ScholarPubMed
24Pool-Zobel, BL, Neudecker, C, Domizlaff, I, Ji, S, Schillinger, U, Rumney, C, Moretti, M, Vilarini, I, Scassellati, SR & Rowland, ILactobacillus and Bifidobacterium mediated antigenotoxicity in the colon of rats. Nutr Cancer 1996 26, 365–380.CrossRefGoogle ScholarPubMed
25Rafter, JJThe role of lactic acid bacteria in colon cancer prevention. Scand J Gastroenterol 1995 30, 497–502.CrossRefGoogle ScholarPubMed
26Reddy, BS & Rivenson, AInhibitory effect of Bifidobacterium longum on colon, mammary and liver carcinogenesis induced by 2-amino-3-methylimidazo [4,5-f] quinoline, a food mutagen. Cancer Res 1993 53, 3914–3918.Google ScholarPubMed
27Reddy, GV, Shaheni, KM, Friend, BA & Chandam, RCNatural antibiotic activity of Lactobacillus acidophilus and bulgaricus. 3. Production and partial purification of bulgarican from Lactobacillus bulgaricus. Cult Dairy Prod J 1983 19, 7–11.Google Scholar
28Rice, LJ, YiJiun, C, Conti, CJ, Willis, RA, Locniskar, MF & Chai, YJThe effect of dietary fermented milk products and lactic acid bacteria on the initiation and promotion stages of mammary carcinogenesis. Nutr Cancer 1995 24, 99–109.CrossRefGoogle ScholarPubMed
29Samona, A & Robinson, RKEnumeration of bifidobacteria in dairy products. J Soc Dairy Technol 1991 44, 64–66.Google Scholar
30SAS Institute, Inc. SAS ® User's Guide: Statistics Cary, NC: SAS Institute, Inc version 6.0. 1990.Google Scholar
31Sekine, K, Toida, T, Saito, M, Kuboyama, M, Kawashima, T & Hashimoto, YA new morphologically characterized cell wall preparation (whole peptidoglycan) from Bifidobacterium infantis with a higher efficacy on the regression of an established tumor in mice. Cancer Res 1985 45, 1300–1307.Google Scholar
32Sekine, K, Watanabe, SE, Ohta, J, Toida, T, Tatsuki, T, Kawashima, T & Hashimoto, YInduction and activation of tumoricidal cells in vivo and in vitro by the bacterial cell wall of Bifidobacterium infantis. Bifid Microflora 1994 13, 65–77.Google Scholar
33Shackelford, LA, Rao, DR, Chawan, CB & Pulusani, SREffect of feeding fermented milk on the incidence of chemically induced colon tumors in rats. Nutr Cancer 1983 5, 159–164.Google Scholar
34Singh, J, Rivenson, A, Tomita, M, Shimamura, S, Ishibashi, N & Reddy, BSBifidobacterium longum, a lactic acid-producing intestinal bacterium inhibits colon cancer and modulates the intermediate biomarkers of colon carcinogenesis. Carcinogenesis 1997 18, 833–841.Google Scholar
35Takano, T, Arai, K, Murota, I, Hayakawa, K, Mizutani, T & Mitsuoka, TEffects of feeding sour milk on longevity and tumorigenesis in mice and rats. Bifid Microflora 1985 4, 31–37.Google Scholar
36Tanteeratarm, K, Nelson, AI & Wei, LSManufacturing of bland soymilk Soybean Extrusion and Soymilk. in Technology, Soy Food Products and Home Utilization. Urbana-Champaign, IL: University of Illinois, International Soybean Program (INTSOY) 1993.Google Scholar