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Influence of the pattern of peptide supply on microbial activity in the rumen simulating fermenter (RUSITEC)

Published online by Cambridge University Press:  09 March 2007

Juan P. Russi ,
R. John Wallace  and
C. James Newbold
Juan P. Russi
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
R. John Wallace
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
C. James Newbold*
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
*
*Corresponding author: Dr C. James Newbold, fax +44 1224 716687, email [email protected]
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Abstract

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The source and pattern of N supply was varied in the rumen simulation technique (RUSITEC) in order to determine if continuous, rather than transient, availability of peptides was required for optimum ruminal fermentation. The energy source was fibre prepared from sugar-beet pulp. N was added as NH3 continuously infused (AC) or peptides (Bacto® Casitone, a pancreatic hydrolysate of casein; Difco Laboratories, Detroit, MI, USA) continuously infused (PC) or added as a single dose at the time of feeding (PS). Free peptides were detected in the fermenter liquid for 4 h after feeding in the AC treatment, for 10 h in the PS treatment, and at all times with the PC treatment. Treatments had no effect on DM degradation. Approximately 40 % of the degradation occurred during the time no peptides were detected in the PS treatment. Microbial N flow tended to be higher with the peptide additions (P<0·061), with no significant difference between the two peptides treatments. The production of liquid-associated micro-organisms (LAM) was higher in the PC treatment (P<0·05) and the proportion of LAM derived from NH3 lower (P<0·05). However, LAM only accounted for 20–30 % total microbial population. Our main conclusion was that peptides had a small stimulatory effect on the fermentation, but there was no indication that synchrony of supply of energy and amino acid-N in the fermenter promoted a more efficient fermentation than non-synchronous supply. This conclusion must be qualified, however, because some N remained in the fibre and may have become available progressively as the fibre was digested by the micro-organisms.

Keywords

SynchronyMicrobial protein synthesisRUSITEC
Type
Research Article
Information
British Journal of Nutrition , Volume 88 , Issue 1 , July 2002 , pp. 73 - 80
Copyright
Copyright © The Nutrition Society 2002

References

Association of Analytical Chemists (1975) Official Methods of Analysis. Washington, DC: AOAC.Google Scholar
Broderick, GA & Wallace, RJ (1988) Effects of dietary nitrogen source on concentrations of ammonia, free amino acids and fluorescamine-reactive peptides in the rumen. Journal of Animal Science 66, 2233–2238.CrossRefGoogle Scholar
Bryant, MP (1972) Commentary on the Hungate technique for culture of anaerobic bacteria. American Journal of Clinical Nutrition 25, 1324–1328.CrossRefGoogle ScholarPubMed
Carro, MD & Miller, EL (1999) Effect of supplementing a fibre basal diet with different nitrogen forms on ruminal fermentation and microbial growth in an in vitro semi-continuous culture system (RUSITEC). British Journal of Nutrition 82, 149–157.CrossRefGoogle Scholar
Chamberlain, DG & Choung, JJ (1995) The importance of rate of ruminal fermentation of energy sources in diets for dairy cows. In Recent Advances in Animal Nutrition, pp. 3–27 [Garnsworthy, PC and Cole, DJA, editors]. Nottingham: Nottingham University Press.Google Scholar
Chen, G, Sniffen, CJ & Russell, JB (1987) Concentration and estimated flow of peptides from the rumen of dairy cattle: effects of protein quantity, protein solubility, and feeding frequency. Journal of Dairy Science 70, 983–992.CrossRefGoogle ScholarPubMed
Chikunya, S, Newbold, CJ, Rode, LM, Chen, XB & Wallace, RJ (1996) Influence of dietary rumen-degradable protein on bacterial growth in the rumen of sheep receiving different energy sources. Animal Feed Science Technology 63, 333–340.CrossRefGoogle Scholar
Conway, EJ (1957) Microdiffusion Analysis and Volumetric Error, 4th ed., pp. 277–278. London: Crosby Lockwood & Son.Google Scholar
Cotta, MA & Russell, JB (1982) Effect of peptides and amino acids on efficiency of rumen bacterial protein synthesis in continuous culture. Journal of Dairy Science 65, 226–235.CrossRefGoogle Scholar
Genstat 5 Committee (1987) Genstat 5 Users' Manual. Oxford: Oxford University Press.Google Scholar
Cruz Soto, R, Muhammed, SA, Newbold, CJ, Stewart, CS & Wallace, RJ (1994) Influence of peptides, amino acids and urea on microbial activity in the rumen of sheep receiving grass hay and on the growth of rumen bacteria in vitro. Animal Feed Science and Technology 49, 151–161.CrossRefGoogle Scholar
Czerkawski, JW & Breckenridge, G (1977) Design and development of a long-term rumen simulation technique (Rusitec). British Journal of Nutrition 38, 371–384.CrossRefGoogle ScholarPubMed
Foss-Heraeus, (1990) Macro-N Nitrogen Analyzer Instruction Manual. York: Foss-Heraeus.Google Scholar
Henderson, AR, Garnsworthy, PC, Newbold, JR & Buttery, PJ (1998) The effect of asynchronous diets on the function of the rumen in the lactating dairy cow. Proceedings of the British Society of Animal Science 1998, 19.CrossRefGoogle Scholar
Henning, PH, Steyn, DG & Meissner, HH (1991) The effect of energy and nitrogen supply pattern on rumen bacteria growth in vitro. Animal Production 53, 165–175.Google Scholar
Henning, PH, Steyn, DG & Meissner, HH (1993) Effect of synchronization of energy and nitrogen supply on ruminal characteristics and microbial growth. Journal of Animal Science 71, 2516–81CrossRefGoogle ScholarPubMed
Hobson, PN (1969) Rumen bacteria. Methods in Microbiology 3B, 133–159.CrossRefGoogle Scholar
Hristov, AN & Broderick, GA (1996) Synthesis of microbial protein in ruminally cannulated cows fed alfalfa silage, alfalfa hay, or corn silage. Journal of Dairy Science 79, 1627–1637.CrossRefGoogle ScholarPubMed
Hume, ID (1970) Synthesis of microbial protein in the rumen. II A response to higher volatile fatty acids. Australian Journal of Agriculture Research 21, 297–304.CrossRefGoogle Scholar
Hungate, RE (1966) The Rumen and its Microbes. Davis, CA: Academic Press.Google Scholar
Johnson, RR (1976) Influence in carbohydrate solubility on non-protein nitrogen utilization in the ruminant. Journal of Animal Science 43, 184–191.CrossRefGoogle ScholarPubMed
Kim, KH, Oh, YG, Choung, JJ & Chamberlain, DG (1999) Effects of varying the degrees of synchrony of energy and nitrogen release in the rumen on the synthesis of microbial protein in cattle consuming grass silage. Journal of Science Food and Agriculture 79, 833–839.3.0.CO;2-C>CrossRefGoogle Scholar
López, S, Valdes, C, Newbold, CJ & Wallace, RJ (1999) Influence of sodium fumarate on rumen fermentation in vitro. British Journal of Nutrition 81, 59–64.CrossRefGoogle ScholarPubMed
Macaulay Land Use Research Institute (2001) Curve fitting Software for in sacco degradability and in vitro gas production data. http://www.miuri.sari.uk/IFRU/Fcurve.htmlGoogle Scholar
McAllan, AB (1991) Carbohydrate and nitrogen metabolism in the fore stomach of steers given untreated or ammonia treated barley straw diets supplemented with urea or urea plus fish meal. Animal Feed Science and Technology 33, 195–208.CrossRefGoogle Scholar
Maeng, WJ & Baldwin, RL (1976) Factors influencing rumen microbial growth rates and yields: effect of amino acids additions to a purified diet with nitrogen from urea. Journal of Dairy Science 59, 648–655.CrossRefGoogle ScholarPubMed
Maeng, WJ, Van Nevel, CJ, Baldwin, RL & Morris, JG (1976) Rumen microbial growth rates and yields: effects of amino acids and proteins. Journal of Dairy Science 59, 68–79.CrossRefGoogle Scholar
Mann, SO (1968) An improved method for determining cellulolytic activity in anaerobic bacteria. Journal of Applied Bacteriology 31, 241–244.CrossRefGoogle Scholar
McDougall, EF (1948) Studies on ruminant saliva. I. The composition and output of sheep's saliva. Biochemical Journal 43, 99–109.CrossRefGoogle ScholarPubMed
Merry, RJ, McAllan, AB & Smith, RH (1990) In vitro continuous culture studies on the effect of nitrogen source on rumen microbial growth and fibre digestion. Animal Feed Science and Technology 31, 55–64.CrossRefGoogle Scholar
Minato, H & Suto, T (1978) Technique for fractionation of bacteria in rumen microbial ecosystem. II. Attachment of bacteria isolated from bovine rumen to cellulose powder in vitro and elution of bacteria attached there from. Journal of General and Applied Microbiology 24, 1–16.CrossRefGoogle Scholar
Newbold, CJ, Williams, AG & Chamberlain, DG (1987) The in vitro metabolism of d,l-lactic acid by rumen microorganisms. Journal of the Science of Food and Agriculture 38, 9–19.CrossRefGoogle Scholar
Newbold, JR & Rust, SR (1992) Effect of asynchronous nitrogen and energy supply on growth of ruminal bacteria in continuous cultures. Journal of Animal Science 70, 538–546.CrossRefGoogle Scholar
Ørskov, ER & McDonald, I (1979) The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science, Cambridge 92, 499–503.CrossRefGoogle Scholar
Rooke, JA & Armstrong, DG (1989) The importance of the form of nitrogen on microbial protein synthesis in the rumen of cattle receiving grass silage and continuous intra-rumen infusions of sucrose. British Journal of Nutrition 61, 113–121.CrossRefGoogle Scholar
Rooke, JA, Brett, PA, Overend, MA & Armstrong, DG (1985) The energetic efficiency of rumen microbial protein synthesis in cattle given silage-based diets. Animal Feed Science and Technology 13, 255–267.CrossRefGoogle Scholar
Salter, DN, Daneshvar, K & Smith, RH (1979) The origin of nitrogen incorporated into compounds in the rumen bacteria of steers given protein and urea-containing diets. British Journal of Nutrition 41, 197–209.CrossRefGoogle ScholarPubMed
Sinclair, LA, Garnsworthy, PC, Newbold, JR & Buttery, PJ (1993) Effect of synchronizing the rate of dietary energy and nitrogen release on rumen fermentation and microbial protein synthesis in sheep. Journal of Agricultural Science 120, 251–263.CrossRefGoogle Scholar
Stewart, CS & Duncan, SH (1985) The effect of avoparcin on cellulolytic bacteria of the ovine rumen. Journal of General Microbiology 131, 427–435.Google Scholar
Van Soest, PJ & Robertson, JB (1985) Analysis of forages and fibrous foods. In Laboratory Manual for Animal Science. Ithaca, NY: Cornell University.Google Scholar
Wallace, RJ (1992) Gel filtration studies of peptide metabolism by rumen microorganisms. Journal of the Science of Food and Agriculture 58, 174–184.CrossRefGoogle Scholar
Wallace, RJ & McKain, N (1990) A comparison of methods for determining the concentration of extracellular peptides in rumen fluid of sheep. Journal of Agricultural Science, Cambridge 114, 101–105.CrossRefGoogle Scholar
Wallace, RJ, Onodera, R & Cotta, MA (1997) Metabolism of nitrogen-containing compounds. In The Rumen Microbial Ecosystem, 2nd ed., pp. 283–328 [Hobson, PN and Stewart, CS, editors]. London: Chapman and Hall.CrossRefGoogle Scholar
Whitehead, R, Cooke, GH & Chapman, BT (1967) Problems associated with the continuous monitoring of ammoniacal nitrogen in river water. Automation in Analytical Chemistry 2, 377–380.Google Scholar