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Influence of sources of dietary vitamin E on the maternal transfer of α-tocopherol to fetal and neonatal guinea pigs as determined by a stable isotopic technique

Published online by Cambridge University Press:  01 August 2008

N. Hidiroglou ,
R. Madere ,
L. R. McDowell  and
P. L. Toutain
N. Hidiroglou*
Affiliation:
Nutrition Research Division, Health Products and Food Branch, Health Canada, Banting Research Center (PL 2203C), Tunneys Pasture, Ottawa, Ontario, Canada K1A-OL2
R. Madere
Affiliation:
Nutrition Research Division, Health Products and Food Branch, Health Canada, Banting Research Center (PL 2203C), Tunneys Pasture, Ottawa, Ontario, Canada K1A-OL2
L. R. McDowell
Affiliation:
University of Florida, Department of Animal Sciences, Gainesville, Florida, USA
P. L. Toutain
Affiliation:
UMR INRA-Ecole Nationale Veterinaire de Toulouse de Physiopathologie et Toxicologie Expérimentales, Toulouse, F 31076, France
*
*Corresponding Author: Dr Nick Hidiroglou, fax +1 613 941 6182, email [email protected]
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Abstract

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The accepted biological potencies of vitamin E (United States Phamacopeia, 1985) for 1 mg all-rac-α-tocopheryl acetate (synthetic form) is 1·00 IU and that of 1 mg (RRR)-α-tocopheryl acetate (natural form) is 1·36 IU. In the present study, a stable isotopic (2H) technique was employed to evaluate the bioavailability of natural v. synthetic forms of vitamin E and to determine whether the potency of the forms is the stated relationship of 1·36:1·00 (RRR)-α-tocopheryl acetate:all-rac-α-tocopheryl acetate. Sixty female in-bred guinea pigs received either 40 or 80 mg vitamin E/kg diet with equal levels of (RRR)-α-tocopheryl acetate and all-rac-α-tocopheryl acetate throughout gestation and lactation. At late-term pregnancy (day 50 or 60) and during early lactation, dams and their corresponding fetuses or neonates were killed and various tissues collected for subsequent α-tocopherol analysis. Vitamin E analysis of fetal and neonatal tissues indicated a substantial transfer of 2H-labelled α-tocopherol across the placenta and through the mammary gland. Total α-tocopherol concentrations were significantly influenced by tissue type and dose level, but not by stage of gestation or lactation. The relative bioavailability (d3:d6) across fetal and neonatal tissues was on average 1·81:1·00, with a range from 1·62:1·00 to 2·01:1·00. Maternal tissues had a mean ratio of 1·77:1·00. A higher relative bioavailability (P≤0·05) was observed with natural compared with synthetic α-tocopherol as shown by a higher d3:d6 ratio in all tissues examined. Vitamin E was highest in colostrum on day 2 then declined through to day 5. Results from this present experiment further question the accepted biological potencies of natural:synthetic α-tocopheryl acetate of 1·36:1·00.

Keywords

Vitamin E sourcesBioavailabilityStable isotopesGuinea pig
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 4 , April 2003 , pp. 455 - 466
Copyright
Copyright © The Nutrition Society 2003

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