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Influence of nitrogen source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal micro-organisms in vitro

Published online by Cambridge University Press:  09 March 2007

M. J. Ranilla ,
M. D. Carro ,
S. López ,
C. J. Newbold  and
R. J. Wallace
M. J. Ranilla*
Affiliation:
Department de Produción Animal I, Campus de Vegazana, Universidad de León, 2407 León, Spain
M. D. Carro
Affiliation:
Department de Produción Animal I, Campus de Vegazana, Universidad de León, 2407 León, Spain
S. López
Affiliation:
Department de Produción Animal I, Campus de Vegazana, Universidad de León, 2407 León, Spain
C. J. Newbold
Affiliation:
Rowett Research Institute, Buckburn, Aberden AB21 9SB, UK
R. J. Wallace
Affiliation:
Rowett Research Institute, Buckburn, Aberden AB21 9SB, UK
*
*Corresponding author: Dr C. L. Girard, fax +1 819 564 5507, email [email protected]
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Abstract

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Incubations were carried out with a batch culture system to study the effects of different N sources on the fermentation by ruminal micro-organisms from Merino sheep of two fibre substrates derived from feedstuffs that differed in their fermentation rate. The substrates were neutral-detergent fibre (NDF) from barley straw and sugarbeet pulp. N sources were ammonia (NH4Cl) and peptides (Trypticase). Three treatments were made by replacing ammonia-N with peptide-N at levels of 0 (AMMO), 33 (PEPLOW) and 66 % (PEPHIGH) of total N. There were no differences (P>0·05) between treatments in NDF degradation for both the barley straw and the sugarbeet pulp. Peptides increased (P<0·05) total volatile fatty acids daily production for both substrates, with greater values (P<0·001) for PEPHIGH than for PEPLOW for the sugarbeet pulp. The presence of peptides also increased (P<0·05) microbial N synthesis compared with AMMO, with PEPHIGH supporting more growth (P<0·001) than PEPLOW when the sugarbeet pulp NDF was fermented. The presence of peptides increased (P<0·01) the amount of solids-associated micro-organisms (SAM)-N for both the barley straw and the sugarbeet pulp fibres, values in the PEPHIGH treatment being higher (P<0·001) than those in PEPLOW. The proportion of SAM-N in the total microbial N was not affected (P>0·05) by the presence of peptides compared with the AMMO treatment, but values were greater for the PEPHIGH compared with the PEPLOW N source, reaching statistical significance (P<0·05) only for the sugarbeet pulp. For liquid-associated micro-organisms, the AMMO treatment resulted in the greatest (P<0·05) proportion of N derived from ammonia for both substrates, with a further decrease (P<0·01) for the PEPHIGH treatment compared with the PEPLOW for the sugarbeet pulp, indicating preferential uptake of peptides when they were available. Microbial growth efficiency (g microbial N/kg NDF degraded) was not affected (P>0·05) by N source. These results indicate that N forms other than ammonia are needed for maximal growth of fibre-digesting ruminal micro-organisms.

Keywords

NitrogenRumen microbial protein sysnthesisBatch culturesSolids-associated micro-organismsliquid-associated micro-organisms
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 6 , December 2001 , pp. 717 - 724
Copyright
Copyright © The Nutrition Society 2001

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