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Influence of different sources of injected selenium on certain enzymes, glutathione and adenosylmethionine concentration in buffalo (Bubalus bubalis) calves

Published online by Cambridge University Press:  09 March 2007

T. Prasad  and
S. P. Arora
T. Prasad
Affiliation:
National Dairy Research Institute, Karnal-132 001, Haryana, India
S. P. Arora
Affiliation:
National Dairy Research Institute, Karnal-132 001, Haryana, India
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Abstract

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Sodium selenite and selenomethionine were investigated as possible causative factors for the induction of Degnala disease syndrome in twelve buffalo (Bubalus bubalis) calves divided into three groups of four. Group 1 was the control group and received no additional selenium. Sodium selenite and selenomethionine were given daily as intramuscular injections on a selenium-equivalent basis, with a weekly increment in the dose of 0.05 mg Se/kg live weight from 0.05 to 0.20 mg Se/kg live weight per day, in groups 2 and 3 respectively. Only one animal from group 3 manifested the lesions of Degnala disease. The blood Se concentration and erythrocyte glutathione peroxidase (EC 1.11.1.9; GSH-Px) activity were both greater in groups 2 and 3 than in control group 1. The overall blood Se concentration was 0.22 (se 0.01), 0.38 (se 0.12) and 0.77 (se 0.20) μg Se/ml in groups 1 to 3 respectively with corresponding GSH-Px activities of 63.84 (se 7.38), 88.37 (se 12.38) and 165.32 (se 40.62) enzyme units/mg protein. Erythrocyte glutathione reductase (NAD(P)H) (EC 1.6.4.2) activity was not affected by treatment but reduced glutathione content was lower in groups 2 and 3. Liver adenosylmethionine, estimated at autopsy, was lowest (22.87 (se 6.17) μmol/g) in group 3, and greatest (102.63 (se 9.39) μmol/g) in group 1 (P < 0.01). Organic Se sources seemed to accumulate in tissues more than inorganic sources, and might be the causative toxic factors of Degnala disease.

Keywords

Glutathione peroxidaseAdenosylmethionineSelenium
Type
Digestion and Utilization of Inorganic Nutrients
Information
British Journal of Nutrition , Volume 66 , Issue 2 , September 1991 , pp. 261 - 267
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Arora, S. P., Kaur, P., Khirwar, S. S., Chopra, R. C. & Ludri, R. S. (1975). Selenium levels in fodders and its relationship with Degnala disease. Indian Journal of Dairy Science 28, 249–253.Google Scholar
Ehlig, C. F., Hogue, D. E., Allaway, W. H. & Hamm, J. (1967). Fate of selenium from selenite or selenomethionine with or without vitamin E in lambs. Journal of Nutrition 92, 121–126.CrossRefGoogle ScholarPubMed
Ganther, H. E., Hafeman, D. G., Lawrence, R. A., Serfass, R. E. & Hoekstra, W. G. (1976). Selenium and glutathione peroxidase in health and disease. In A Review on Trace Elements in Human Health and Disease, vol. 2, pp. 165–234 [Prasad, A. S., editor]. New York: Academic Press.Google Scholar
Ganther, H. E. & Hsieh, H. S. (1974). Mechanisms for the conversion of selenite to selenides in mammalian tissues. In Trace Element Metabolism in Animals, vol. 2, pp. 337–354 [Hoekstra, W. G., Suttie, J. W., Ganther, H. E. and Mertz, W., editors]. Baltimore: University Park Press.Google Scholar
Hafeman, D. G., Sunde, R. A. & Hoekstra, W. G. (1974). Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. Journal of Nutrition 104, 580–587.CrossRefGoogle ScholarPubMed
Hoffman, J. L. (1975). Selenium toxicity and methyl group metabolism. Federation Proceedings 34, 924 Abstr. 3991.Google Scholar
Hoffman, J. L. (1977) Selenite toxicity, depletion of liver S-adenosylmethionine, and inactivation of methionine adenosyltransferase. Archives of Biochemistry and Biophysics 179, 136–140.CrossRefGoogle ScholarPubMed
Hsieh, H. S. & Ganther, H. E. (1975). Acid volatile selenium formation catalysed by glutathione reductase. Biochemistry 14, 1632–1636.CrossRefGoogle Scholar
Khirwar, S. S. & Arora, S. P. (1975). Tissue distribution and excretion pattern of 75Se in animals. Journal of Nuclear Agriculture and Biology 4, 5–8.Google Scholar
Khirwar, S. S. & Arora, S. P. (1977). Incorporation of 75Se-Selenomethionine in milk proteins of goats. Milchwissenschaft 32, 283–285.Google Scholar
King, J. (1965). Practical Clinical Enzymology, pp. 70–74. London: Van Norstrend Company Ltd.Google Scholar
Kraus, R. J., Stephen, J. F. & Ganther, H. E. (1983). Identification of selenocysteine in glutathione peroxidase by mass spectroscopy. Biochemistry 22, 5853–5858.Google Scholar
Lee, M., Doug, A. & Yano, J. (1969). Metabolism of 75Se-selenite by human whole blood in vitro. Canadian Journal of Biochemistry 47, 791–797.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.Google Scholar
Mathias, M. M., Hogue, D. E. & Loosli, J. K. (1967). The biological value of selenium in bovine milk for the rat and chick. Journal of Nutrition 93, 14–20.Google Scholar
Ochoa-Solano, A. & Gitler, C. (1968). Incorporation of 75Se-selenomethionine and 35S-methionine into chicken egg white proteins. Journal of Nutrition 94, 243–248.Google Scholar
Olson, O. E., Palmer, I. S. & Carry, E. E. (1975). Modification of official fluorimetric method for selenium in plants. Journal of the Association of Official Analytical Chemists 58, 117–121.Google Scholar
Prasad, T. & Arora, S. P. (1980). 75Se accumulation in rice plants and its effect on yield. Journal of Nuclear Agriculture and Biology 9, 77–78.Google Scholar
Prasad, T. & Arora, S. P. (1984). Influence of feeding high and low selenium rice straw on erythrocyte glutathione peroxidase activity and blood selenium concentration of buffalo calves. Indian Journal of Animal Nutrition 1, 1–4.Google Scholar
Prins, H. K. & Loos, J. A. (1969). Glutathione. In Biochemical Methods in Red Cell Genetics, pp. 115–137 [Yunis, G. J., editor]. New York: Academic Press.Google Scholar
Rosenfeld, I. & Beath, O. A. (1964). Selenium: Geobotany, Biochemistry, Toxicity and Nutrition, pp. 130–169. New York: Academic Press.Google Scholar
Rowell, J. G. & Walters, D. E. (1976). Analysing data with repeated observations on each experimental unit. Journal of Agricultural Science, Cambridge 87, 423–432.CrossRefGoogle Scholar
Salvatore, E., Utili, R., Zappa, V. & Shapiro, S. K. (1971). Quantitative analysis of S-adenosylmethionine and S-adenosylhomocysteine in animal tissues. Analytical Biochemistry 41, 16–28.CrossRefGoogle ScholarPubMed
Sen, K. C., Ray, S. N. & Ranjhan, S. K. (1978). Nutritive Value of Indian Feeds and Feeding of Animals. Indian Council of Agricultural Research Bulletin no. 25. New Delhi: Indian Council of Agricultural Research, Naba Mudran Pvt. Ltd. Press.Google Scholar
Snedecor, G. W. & Cochran, W. B. (1968). Statistical Methods, 7th ed.. Ames: Iowa State University Press.Google Scholar
Tekchandani, N. & Arora, S. P. (1978). Incorporation of 75Se-selenomethionine in rabbit tissue proteins and rumen microbial proteins in the cow. Indian Journal of Dairy Science 31, 345–349.Google Scholar