Vitamin E is a potent lipid-soluble antioxidant that is only synthesised by plants and cyanobacteria, and therefore it is essential for human and animal nutrition(Reference Schneider1). In nature, compounds with vitamin E activity include α-, β-, γ- and δ-tocopherols (TCP) as well as α-, β-, γ- and δ-tocotrienols (TCT). The molecular structure of TCT differs from the corresponding TCP in their aliphatic tail containing an unsaturated isoprenoid chain, whereas the tail of TCP has a saturated phytyl chain(Reference Schneider1). TCT are minor plant constituents especially abundant in palm oil, cereal grains and rice bran, all providing significant sources of vitamin E activity(Reference Ong, Packer and Fuchs2). The antioxidant properties of TCT have been well investigated by several in vitro experiments and seem to be higher than those of α-TCP(Reference Serbinova, Kagan and Han3, Reference Begum and Terao4). Recently, TCT have gained increasing scientific interest mainly by the discovery of their non-antioxidant actions, which include neuroprotective, anti-carcinogenic and cholesterol-lowering properties(Reference Packer, Weber and Rimbach5, Reference Sen, Khanna and Roy6). These properties of TCT have also spurred interest in determining their ability to prevent degenerative diseases(Reference Theriault, Chao and Wang7). However, TCT metabolism in dogs or other companion animals is not known. Therefore, the present study was conducted to measure the intestinal absorption of TCT and antioxidant capacity in the plasma of dogs receiving a single oral dosage of TCT.
Experimental methods
Animals and experimental design
A total of eight adult Beagle dogs, age ranging from 3 to 6 years (average 3·8 years), were enrolled in the present study according to the guidelines for animal welfare of the German Society of Experimental Animal Science. The study protocol was approved by the local Animal Welfare Committee (Referat 74; Regierungspräsidium, Leipzig, Germany). Dogs were fed a commercial dry dog food (Altromin breeding and maintenace diet for dogs #4130; Altromin Spezialfutter GmbH, Lage, Germany) formulated to meet the nutrient recommendations of the National Research Council for adult dogs(8). Crude nutrient and energy content of the basal diet per kg were as follows: DM (880 g); crude protein (240 g); crude fat (55 g); crude fibre (40 g); crude ash (95 g); N-free extract (450 g); metabolisable energy (12·5 MJ); Ca (14 g); P (11 g); Mg (2 g); Na (2 g); K (9 g); vitamin A (4500 μg retinol equivalents); vitamin D3 (15 μg); vitamin E (as α-tocopherol acetate, 75 mg). Dogs had free access to tap water. Following an overnight fast, the animals received an oral bolus of 40 mg/kg body weight of a tocotrienol-rich fraction (TRF) extracted from palm oil (Gold Tri E; Golden Hope Plantations Berhard, Kuala Lumpur, Malaysia) containing 32 % α-TCT, 2 % β-TCT, 27 % γ-TCT, 14 % δ-TCT and 25 % α-TCP, together with 5 ml cream (fat, 30 %; α-TCP, 0·9 μg/ml). A baseline blood sample was collected from the cephalic vein into EDTA-evacuated tubes immediately before administration of the TRF, and subsequent serial blood samples were collected at 1, 2, 3, 4, 5, 6, 8 and 12 h post-dosage.
Analytical methods
Plasma was prepared by centrifugation of the blood samples (1500 g; 10 min at 4°C), and the chylomicrons were isolated by ultracentrifugation (30 min, 100 000 g at 10°C) from 1 ml fresh plasma at density < 1·006 g/ml(Reference Havel, Eder and Bragdon9). Plasma and chylomicrons were organically extracted with n-hexane stabilised with 0·05 % butylated hydroxytoluene for measuring their TCT and α-TCP concentrations, and were separated using a modified gradient RP-HPLC system (Waters, Eschborn, Germany) with a C30 column (5 μm, 250 × 4·6 mm; YMC, Wilmington, NC, USA) as described elsewhere(Reference Raila, Willnow and Schweigert10). α-, β-, γ- and δ-TCT and α-TCP were identified by comparing the retention time and the peak area with external standards (BASF, Ludwigshafen, Germany) using a Waters 474 fluorescence detector (excitation 290 nm, emission 330 nm). All solvents or chemicals used for extraction or HPLC were of high-purity commercial grade (Roth, Karlsruhe, Germany). Plasma TAG concentrations were measured using a commercially available enzymatic colorimetric assay kit (Thermo Electron, Melbourne, VIC, Australia). The total antioxidant status in plasma was determined by the Trolox equivalent antioxidant capacity assay as described elsewhere(Reference Carlsohn, Rohn and Bittmann11).
Statistical analysis
Results are expressed as means and standard deviations. Variations in the response variable were partitioned using the general linear model procedure of SPSS (version 15.0; SPSS Inc., Chicago, IL, USA) for repeated-measures design. The maximum plasma concentration and the time to reach maximum plasma concentration were obtained directly from the plasma values. Statistical significance was accepted at P < 0·05.
Results
Male dogs (n 4) weighed 14·3 (sd 1·06) kg and female dogs (n 4) weighed 12·7 (sd 1·33) kg. Total plasma TCT concentrations (sum of α-, β-, γ- and δ-TCT) at baseline (0 h) were only detectable in three out of eight dogs and gave a mean value of 0·07 (sd 0·03) μmol/l. As a consequence of the administration of 40 mg TRF/kg body weight, plasma total TCT concentrations peaked at 2 h and reached the highest values (P < 0·01) ranging between 5·69 and 18·11 μmol/l (Fig. 1(a)). At this time point, α-TCT (43 (sd 12) %) as well as β- and γ-TCT (43 (sd 7) %) were the predominant form of TCT followed by δ-TCT (13·3 (sd 6·35) %). Starting from 2 h and beyond, plasma TCT content was significantly higher (P < 0·01) than the baseline levels throughout the postprandial intervention, and at 12 h post-TRF dosage, total TCT levels were still significantly higher than the baseline levels (0·67 (sd 0·44) μmol/l; P < 0·01). Plasma α-TCP was the major circulating vitamin E isomer at baseline (128 (sd 21·3) μmol/l) and remained constant during the entire 12 h study interval. The total TCT response in the chylomicrons (Fig. 1(b)) paralleled the increase in TCT observed in plasma with a peak at 2 h (3·49 (sd 2·06) μmol/l; P < 0·01) and was accompanied with the maximum increment in plasma TAG (Fig. 1(c)). The total antioxidant capacity in plasma measured as Trolox equivalents (Fig. 1(d)) increased continuously from 2 h post-TRF dosage (776 (sd 51·2) μmol/l) to maximum concentrations at 12 h (1130 (sd 77·2) μmol/l) (P < 0·01).
Fig. 1 Postprandial changes in α-tocotrienols (TCT, ●), β- + γ-TCT (○) and δ-TCT (▲) in (a) plasma and (b) chylomicrons as well as (c) TAG (■) in plasma and (d) Trolox equivalents (♦) in the plasma of eight dogs measured after the intake of a tocotrienol-rich fraction (40 mg/kg body weight). Values are means, with standard deviations represented by vertical bars. *Mean values were significantly higher relative to the baseline levels on the basis of the general linear model procedure of SPSS (t = 0; P < 0·05).
Discussion
To our knowledge, the present results demonstrate for the first time that dogs are capable of absorbing orally ingested TCT from a TRF obtained from palm oil as the plasma and chylomicron α-, β-, γ- and δ-TCT levels increased throughout the postprandial period. The maximum increment in TCT concentrations in plasma and chylomicrons was paralleled by peaked TAG, suggesting that intestinal TCT absorption in dogs is processed similarly as other dietary lipids, although no direct evidence is available. Moreover, the distribution of the TCT isomers at the time of their maximal plasma concentrations reflects the percentage distribution of TCT in the palm oil TRF supplement. This result suggests that in dogs, the intestinal absorption rate is not different among α-, β-, γ- and δ-TCT, which seems to be the same situation as in human subjects(Reference O'Byrne, Grundy and Packer12–Reference Fairus, Nor and Cheng14), but it is in contrast to rats, in which α-TCT is absorbed preferentially compared with γ- and δ-TCT(Reference Ikeda, Imasato and Sasaki15, Reference Yap, Yuen and Lim16).
In the present study, α-TCP was the major vitamin E isomer in plasma, even when dogs were challenged with a preparation whose composition was high (75 %) in TCT. This finding is in agreement with previous studies that examined the effect of TCT supplementation in human subjects(Reference O'Byrne, Grundy and Packer12, Reference Fairus, Nor and Cheng14, Reference Mensink, van Houwelingen and Kromhout17) and might be due to a rapid replacement of α-TCP in plasma lipoproteins by newly absorbed α-TCP from the gut(Reference Traber, Rader and Acuff18). Thus, it was concluded that the capacity to increase plasma α-TCP concentrations is limited(Reference Brigelius-Flohe, Kelly and Salonen19). Moreover, it is well established that intestinal chylomicrons are converted to remnant particles that are subsequently taken up by the liver, where newly absorbed vitamin E is incorporated into nascent VLDL(Reference Traber, Burton and Hamilton20). Of the various isomers of dietary vitamin E, α-TCP is preferentially secreted by the liver into the plasma VLDL mediated by α-tocopherol transfer protein (TTP)(Reference Traber, Burton and Hamilton20, Reference Schaffer, Muller and Eckert21). By contrast, the transfer of TCT into VLDL may be independent of TTP function, because TTP has a comparatively poor efficiency to transport TCT than α-TCP(Reference Hosomi, Arita and Sato22). On the other hand, feeding experiments in rats have shown that oral TCT not only reach the plasma but also accumulate in considerable amounts in several tissues including the heart, lung and skin(Reference Khanna, Patel and Rink23). In addition, a long-term feeding trial has demonstrated that dietary α-TCT can be detected in plasma and all tissues of rats fed a diet containing α-TCT for 8 weeks(Reference Ikeda, Tohyama and Yoshimura24). Thus, it is at present not clear whether, or to what extent, the delivery of orally supplemented TCT to peripheral tissues is dependent on the function of TTP(Reference Sen, Khanna and Roy6). Another explanation of the lower affinity of TCT for α-TTP is the rapid degradation of TCT to water-soluble carboxychromans and subsequent excretion into urine(Reference Brigelius-Flohe25). β- and γ-TCT rather than α-TCT may be preferentially metabolised(Reference Birringer, Pfluger and Kluth26), which would explain the more rapid decline in plasma β- and γ-TCT concentrations in the present study.
All vitamin E isomers are well known for their antioxidant properties(Reference Sen, Khanna and Roy6). Structurally, TCT differ from TCP by the presence of the unsaturated isoprenoid side chain and are thought to be more potent antioxidants than α-TCP(Reference Serbinova, Kagan and Han3, Reference Begum and Terao4). The antioxidant ability of TCT and TCP was mainly investigated in several in vitro experiments(Reference Schaffer, Muller and Eckert21). In the present study, the total plasma antioxidant capacity was measured in vivo and a continuous increase in Trolox equivalents throughout the postprandial intervention was found, while the concentrations of total TCT already peaked at 2 h post-dosage. This result seems to contradict with the assumption that TCT are able to enhance plasma antioxidant capacity. However, with the data obtained in the present study, it is difficult to address the mechanism of the antioxidant action of TCT in vivo, because the transport and exchange of TCT among lipoproteins, the presence of carrier proteins specific for TCT and especially the incorporation of TCT into the target cells remain unknown in dogs. Moreover, TCT are able to activate the nuclear pregnane X receptor and thus regulate the expression of several target genes that are involved in the antioxidant defence system including glutathione S-transferase(Reference Landes, Pfluger and Kluth27). The delayed activity of such antioxidant-active enzymes may be an explanation for the observed time differences between the maximum TCT and Trolox equivalent concentrations in the plasma of dogs.
In summary, the present results show that dogs are able to absorb α-, β-, γ- and δ-TCT isomers after the oral administration of a TRF obtained from palm oil. This result and the observed increase in total plasma antioxidant capacity provide significant information regarding the biopotency of TCT isomers in dogs. The supplementation with TCT may therefore be considered in the prevention and treatment of several degenerative diseases that are accompanied with increased oxidative stress.
Acknowledgements
The present study received no specific grant from any funding agency in the public, commercial or not-for-profit sectors. All authors contributed to the conception and design of the study, the acquisition and interpretation of the data and writing of the manuscript. There is no conflict of interest for any of the authors.
