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The hypocholesterolaemic effects of pectins in rats

Published online by Cambridge University Press:  24 July 2007

Patricia A. Judd  and
A. S. Truswell
Patricia A. Judd
Affiliation:
Department of Food Science and Nutrition, Queen Elizabeth College, Campden Hill Road, London W8 7AH
A. S. Truswell
Affiliation:
Department of Food Science and Nutrition, Queen Elizabeth College, Campden Hill Road, London W8 7AH
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Abstract

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1. The hypocholesterolaemic effects of pectins were studied in a series of five experiments in weanling Sprague-Dawley rats.

2. Expt A examined the effects of differing levels of dietary fat on plasma and liver lipids. Rats were given diets containing 100 g pectin, National Formulary (NF)/kg, and either 50,100, 150 or 200 g oil/kg. All diets contained 10 g cholesterol/kg. Plasma cholesterol levels were lower in all pectin-fed groups compared with rats given the control diet containing 100 g Solkafloc and 100 g oil/kg. Liver lipid and cholesterol levels increased with increasing fat content of the diet, reaching a plateau at 150 g fat/kg diet, but were still significantly lower in all pectin-fed groups compared with the control group.

3. Expt B. The effects of molecular weight and degree of methoxylation of pectins were studied in five groups of rats given either a control diet containing 100 g Solkafloc/kg or high molecular weight, high methoxyl pectin (HMW HMP); high molecular weight, low methoxyl pectin (HMW LMP); low molecular weight, high methoxyl pectin (LMW HMP); low molecular weight, low methoxyl pectin (LMW LMP). All diets in this and subsequent experiments contained 100 g fat and 10 g cholesterol/kg. Plasma cholesterol levels were significantly lower than control values only in the HMW HMP group. Compared with controls, animals given HMW pectins had lower levels of liver lipid and liver cholesterol; on the LMW HMP diet the liver cholesterol, but not the liver lipid, was lower.

4. Expt C. An attempt was made to clarify the possible effect of degree of methoxylation by feeding diets containing either 100 g Solkafloc/kg,100 g pectin NF/kg or 100 g very high methoxyl pectin/kg.Plasma cholesterol levels were significantly reduced by both pectins but there was no difference in effect between the two. Both had similar viscosities suggesting that this is a more important factor than methoxyl content.

5. Expts D and E. Effects of dose on hypocholesterolaemic effects of HMP and LMP were studied. Diets containing 50 or 100 g Solkafloc, HMP or LMP/kg were given in Expt D, and 25 g Solkafloc or HMP/kg, 50 g Solkafloc, HMP or LMP/kg and 100 g Solkafloc or LMP/kg in Expt E. Plasma cholesterol levels were significantly reduced in groups given 50 or 100 g HMP/kg and in groups given 100 g LMP/kg.

6.HMP were found to be more effective at lowering plasma cholesterol levels than LMP. LMW pectins were not effective. This suggests that the hypocholesterolaemic effects are at least partly due to viscosity.

7. Gut length and weight was increased in pectin-fed animals compared with controls despite their lower body-weight. The weight of small intestinal contents at death was also greater in pectin-fed rats (Expts A and B), particularly in the distal small intestine.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 53 , Issue 3 , May 1985 , pp. 409 - 425
Copyright
Copyright © The Nutrition Society 1985

References

Abell, L. L., Levy, B. B., Brodie, B. B. & Kendall, F. E. (1959). Journal of Biological Chemistry 195, 357–366.CrossRefGoogle Scholar
Addis, T. (1931–1932). American Journal of Physiology, 99, 415–423.Google Scholar
American national formulary XIII (1970). Pectin. Monograph no. 525.Google Scholar
Anderson, T. A. & Bowman, R. D. (1969). Proceedings of the Society of Experimental Biology and Medicine 130, 665–666.CrossRefGoogle Scholar
Bradstreet, B. B. (1965). The Kjeldahl Method for Organic Nitrogen. New York and London: Academic press.Google Scholar
Brown, R. C., Kelleher, J. & Losowsky, M. S. (1978). Gut 19, A45–A454.Google Scholar
Brown, R. C., Kelleher, J., Walker, B. L. & Losowsky, M. S. (1979). British Journal of Nutrition 41, 455–464.CrossRefGoogle Scholar
Cassidy, M. M., Lightfoot, F. G., Grau, L. E., Story, J. A., Kritchevsky, D. & Vahouney, G. V. (1981). American Journal of Clinical Nutrition 34, 218–228.CrossRefGoogle Scholar
Chang, M. L. W. & Johnson, M. (1976). Journal of Nutrition 106, 1562–1568.CrossRefGoogle Scholar
Cumrnings, J. H., Southgate, D. A. T., Branch, W. J., Wiggins, H. S., Houston, H., Jenkins, D. J. A., Jivraj, T. & Hill, M. J. (1979). British Journal of Nutrition 41, 477–485.CrossRefGoogle Scholar
Duncan, D. B. (1955). Biometrics. 11, 1–42.CrossRefGoogle Scholar
Eastwood, M. A. & Hamilton, D. (1968). Biochimica et Biophysica Acta 152, 165–173.CrossRefGoogle Scholar
Eastwood, M. A. & Mowbray, L. (1976). American Journal of Clinical Nutrition 29, 1461–1467.CrossRefGoogle Scholar
Ershoff, B. H. & Wells, A. F. (1962). Excerpta Medica Surgica 20, 272–276.Google Scholar
Ershoff, B. H. & Wells, A. F. (1967). Proceedings of the Society for Experimental Biology and Medicine 124, 749–752.Google Scholar
Fausch, H. D. & Anderson, T. A. (1965). Journal of Nutrition 85, 145–148.CrossRefGoogle Scholar
Fisher, H., Griminger, P. & Siller, W. G. (1967). Journal of Atherosclerosis Research 7, 381–386.CrossRefGoogle Scholar
Fisher, H., Griminger, P., Weiss, H. S. & Siller, W. G. (1964). Science 146, 1063–1064.CrossRefGoogle Scholar
Folch, J., Lees, M. & Sloane-Stanley, G. H. (1957). Journal of Biological Chemistry 226, 497–509.CrossRefGoogle Scholar
Food and argriculture organization/world health organization (1975). Fao nutrition report series, no. 55/A. Rome/geneva:Fao/who.Google Scholar
Hove, E. L. & King, S. (1979). Journal of Nutrition 109, 1274–1279.CrossRefGoogle Scholar
Hyun, S. A., Vahouney, G.V. & Threadwell, C. R. (1963). Proceedings of the Society for Experimental Biology and Medicine 112, 496–500.CrossRefGoogle Scholar
Judd, P. A., Kay, R. M. & Truswell, A. S. (1977). Nutrition and Metabolism 21 (Suppl. 1 ), 84–85.CrossRefGoogle Scholar
Kay, R. M., Judd, P. A. & Truswell, A. S. (1978). American Journal of Clinical Nutrition 31, 562–563.CrossRefGoogle Scholar
Kay, R. M. & Truswell, A. S. (1977). American Journal of Clinical Nutrition 30, 171–175.CrossRefGoogle Scholar
Kelley, J. J. & Tsai, A. C. (1978). Journal of Nutrition 108, 630–639.CrossRefGoogle Scholar
Kertesz, Z.I. (1951). The pectic substances. New york: Interscience.Google Scholar
Kertesz, Z. I. (1963). In Comprehensive biochemistry, vol. V, pp. 233–245 [Florkin, M and Stotz, E. H. editors].New york: Elsevier.Google Scholar
Kim, K. S. & Ivy, A. C. (1952). American Journal of Physiology 171, 302–318.CrossRefGoogle Scholar
Kiriyama, S., Okasaki, Y. & Yoshida, A. (1969). Journal of Nutrition 97, 382–388.CrossRefGoogle Scholar
Leeds, A. R., Bolster, N. R., Andrews, R. & Truswell, A. S. (1979). Proceedings of the Nutrition Society 38, 44A..CrossRefGoogle Scholar
Leveille, G. A. & Sauberlich, H. E. (1966). Journal of Nutrition 88, 209–213.CrossRefGoogle Scholar
Lin, T. M., Kim, K. S., Karvinen, E. & Ivy, A. C. (1957). American Journal of Physiology 188, 66–70.CrossRefGoogle Scholar
McIntyre, N., Kirsh, K., Orr, J. C. & Isselbacher, K. J. (1971). Journal of Lipid Research 12, 336–346.CrossRefGoogle Scholar
Mckay, J. E., Stainsby, G. & Stirrup, J. E. (1969). Pectin – a review of publications on viscosity and molecular weight. Hereford: H. p. bulmer ltd.Google Scholar
Miller, D. S. & Payne, P. R. (1959). British Journal of Nutrition 13, 501–508.CrossRefGoogle Scholar
Mokady, S. (1973). Nutrition and Metabolism 15, 290–294.CrossRefGoogle Scholar
Mokady, S. (1974). Nutrition and Metabolism 16, 203–207.CrossRefGoogle Scholar
Pilnik, W.&Voragen, A. G. J. (1970). In The Biochemistry of Fruits and Their products, pp. 53–65 [Hulme, A. A. editor]. London: Academic press.Google Scholar
Rees, D. A. (1967). The shapes of molecules: Carbohydrate Polymers. Contemporary Science Paperbacks. Edinburgh: Oliver and boyd.Google Scholar
Riccardi, B. A. & Fahrenbach, M. J. (1967). Proceedings of the Society for Experimental Biology and Medicine 124, 749–752.CrossRefGoogle Scholar
Scott, S. (1978). Investigations into the effects of dietary fibre on the morphology of the gastrointestinal tract of the rat. msc thesis, Queen elizabeth college, University of london.Google Scholar
Tsai, A. C., Elias, J., Kelly, J. M., Lin, R. S. & Robson, J. R. K. (1976). Journal of Nutrition 106, 18–23.Google Scholar
Viola, S., Zimmerman, G. & Mokady, S. (1970). Nutrition Reports International 1, 367–375.Google Scholar
Wells, A. F. & Ershoff, B. H. (1961). Journal of Nutrition 74, 87–92.CrossRefGoogle Scholar
Wells, A. F. & Ershoff, B. H. (1962). Proceedings of the Society for Experimental Biology and Medicine 111, 147–149.CrossRefGoogle Scholar
Werch, S. C. & Ivy, A. C. (1941). American Journal of Digestive Diseases 8, 101–105.CrossRefGoogle Scholar
Williams, R. D. & Olmstead, W. H. (1938). Journal of Nutrition 11, 433–449.CrossRefGoogle Scholar
Younoszai, M. K., Adedoyin, M. & Ranshaw, J. (1978). Journal of Nutrition 108, 341–350.CrossRefGoogle Scholar