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The hypercholesterolaemic effect of caffeine in rats fed on diets with and without supplementary cholesterol

Published online by Cambridge University Press:  24 July 2007

R. Fears
Affiliation:
Beecham Pharmaceuticals Research Division, Nutritional Research Centre, Walton Oaks, Tadworth, Surrey KT20 7NT
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Abstract

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1. The effect of caffeine (2.5 g/kg diet) on lipid metabolism was examined in rats fed on a stock (low-cholesterol) diet or on a cholesterol plus cholic acid-supplemented (high-cholesterol) semi-synthetic diet.

2. When caffeine was included in the stock diet fed to rats for 7 d, there was a moderate but significant increase in the concentration of serum cholesterol compared to the levels observed in the control rats. This change can be accounted for by the increase that was observed in the rate of cholesterogenesis in the liver.

3. After 25 d of caffeine in the stock diet, hepatic cholesterogenesis was still increased but the concentration of serum cholesterol was now the same as in the control rats. During the experimental period there was a progressive increase in the faecal excretion of neutral sterols in the rats receiving caffeine.

4. When caffeine was added to a cholesterol plus cholic acid-supplemented diet, there was a marked increase in the concentration of serum cholesterol but hepatic cholesterogenesis was now reduced.

5. Caffeine in the high-cholesterol diet appeared to delay, but probably did not reduce, the absorption of an oral dose of radio-labelled cholesterol. This conclusion was confirmed using rats which had not previously received either caffeine or cholesterol in the diet.

6. When the effect of caffeine in the high-cholesterol diet was investigated during a 24 h period, an exacerbation of the hypercholesterolaemia was seen only at certain times.

7. After a 4-month period of feeding rats on the caffeine-supplemented high-cholesterol diet, histological examination did not detect any damage to the heart and aorta.

8. The metabolic regulations involved in the effects of caffeine in the two diets are discussed and the relevance of the present results to observations made with human subjects is considered.

Type
Papers of direct relevance to Clinical and Human Nutrition
Copyright
Copyright © The Nutrition Society 1978

References

REFERENCES

Akinyanju, P. & Yudkin, J. (1967). Nature, Lond. 214, 426.CrossRefGoogle Scholar
Axelrod, J. & Reichenthal, J. (1953). J. Pharmac. exp. Ther. 107, 519.Google Scholar
Balasubramaniam, S., Goldstein, J. L., Faust, J. R. & Brown, M. S. (1976). Proc. natn. Acad. Sci. U.S.A. 73, 2564.CrossRefGoogle Scholar
Bellet, S., Roman, L., DeCastro, O., Kim, K. E. & Kershbaum, A. (1969). Metabolism 18, 288.CrossRefGoogle Scholar
Bligh, E. G. & Dyer, W. J. (1959). Can. J. Biochem. 37, 911.Google Scholar
Bortz, W. M. (1968). Biochim, biophys. Acta 152, 619.Google Scholar
Cholewa, J. (1973). Materia med. Polona 5, 235.Google Scholar
Dawber, T. R., Kannel, W. B. & Gordon, T. (1974). New Engl. J. Med. 291, 871.Google Scholar
Diplock, A. T., Green, J., Bunyan, J., McHale, D. & Muthy, I. R. (1967). Br. J. Nutr. 21, 115.CrossRefGoogle Scholar
Dominguez, M. C. & Herrera, E. (1976). Horm. Metab. Res. 8, 33.CrossRefGoogle Scholar
Duncombe, W. G. (1963). Biochem. J. 88, 7.CrossRefGoogle Scholar
Edwards, P. A. (1975). Archs Biochem. Biophys. 170, 188.Google Scholar
Ehret, C. F., Potter, V. R. & Dobra, K. W. (1975). Science, N.Y. 188, 1212.Google Scholar
Fears, R. (1973). Adaptations in the metabolism of protein during pregnancy, and their nutritional implications. PhD thesis, University of London.Google Scholar
Fears, R. & Morgan, B. (1976). Biochem. J. 158, 53.Google Scholar
Feldman, J. F. (1975). Science, N. Y. 190, 789.CrossRefGoogle Scholar
Folch, J., Lees, M. & Sloane-Stanley, G. H. (1957). J. biol. Chem. 226, 497.CrossRefGoogle Scholar
Frantsuzova, S. B. (1975). Byull. eksp. Biol. Med. 79, 68.Google Scholar
Hennekens, C. H., Drolette, M. E., Jesse, M. J., Davies, J. E. & Hutchison, G. B. (1976). New Engl. J. Med. 294, 633.CrossRefGoogle Scholar
Itaya, K. & Ui, M. (1965). J. Lipid Res. 6, 16.Google Scholar
Jeanrenaud, B. (1968). Ergebn. Physiol. 60, 57.Google Scholar
Mann, H. B. & Whitney, D. R. (1947). Ann. math. Statist. 18, 52.Google Scholar
Mann, J. I. & Thorogood, M. (1975). Lancet ii, 1215.CrossRefGoogle Scholar
Marks, V. (1974). Proc. Nutr. Soc. 33, 209.Google Scholar
Miettinen, T. A., Ahrens, E. H. Jr & Grundy, S. M. (1965). J. Lipid Res. 6, 411.Google Scholar
Moore, M. C., Guzman, M. A., Schilling, P. E. & Strong, J. P. (1975). J. Am. diet. Ass. 67, 22.CrossRefGoogle Scholar
Morgan, B., Heald, M., Atkin, S. D., Green, J. & Chain, E. B. (1974). Br. J. Nutr. 32, 447.CrossRefGoogle Scholar
Murphy, B. E. P. (1967). J. clin. Endocr. Metab. 27, 973.Google Scholar
Myasnikov, A. L. (1958). Circulation 17, 99.Google Scholar
Naidoo, S. S., Lossow, W. J. & Chaikoff, I. L. (1962). J. Lipid Res. 3, 309.CrossRefGoogle Scholar
Naismith, D. J., Akinyanju, P. A., Szanto, S. & Yudkin, J. (1970). Nutr. Metab. 12, 144.CrossRefGoogle Scholar
Naismith, D. J., Akinyanju, P. A. & Yudkin, J. (1969). J. Nutr. 97, 375.Google Scholar
Nichols, A. B., Ravenscroft, C., Lamphier, D. E. & Ostrander, L. D. (1976). J. Am. med. Ass. 236, 1948.Google Scholar
Oberman, Z., Herzberg, M., Jaskolka, H., Harell, A., Hoerer, E. & Laurian, L. (1975). Israel J. med. Sci. 11, 33.Google Scholar
Shafrir, E., Sussman, K. E. & Steinberg, D. (1959). J. Lipid Res. 1, 109.CrossRefGoogle Scholar
Shrivastava, B. K. (1965). Indian Heart J. 17, 234.Google Scholar
Siegel, A. L. & Bowdoin, B. C. (1971). Clin. Chem. 17, 229.CrossRefGoogle Scholar
Technicon Instruments Corp. (1965). Laboratory Method Files N 24A, N78. Tarrytown, New York: Technicon Instruments Corp.Google Scholar
Truswell, A. S. (1974). Proc. Nutr. Soc. 33, 215.Google Scholar
Vost, A. & Hollenberg, C. H. (1969). Lancet ii, 219.Google Scholar
Wald, A. & Bayless, T. M. (1975). Gastroenterology 68, 1008.CrossRefGoogle Scholar
Waldeck, B. (1975). Acta pharmac. tox. 36, Suppl. 4, 1.CrossRefGoogle Scholar