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The hydrogenation of some cis- and trans-octadecenoic acids to stearic acid by a rumen Fusocillus sp.

Published online by Cambridge University Press:  09 March 2007

Patrick Kemp ,
David J. Lander  and
Frank D. Gunstone
Patrick Kemp
Affiliation:
AFRC Institute of Animal Physiology, Babraham, Cambridge CB2 4AT
David J. Lander
Affiliation:
AFRC Institute of Animal Physiology, Babraham, Cambridge CB2 4AT
Frank D. Gunstone
Affiliation:
Department of Chemistry, The University, St Andrews, Fife, Scotland
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Abstract

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1. The hydrogenation of a range of double-bond positional and configurational octadecenoic acid isomers (cis (δ2 and δ4 to δ13) and trans (δ2 and δ5 to δ13)) to stearic acid by a rumen Fusocillus sp. were examined.

2. The cis and trans δS to δ13 isomers were all hydrogenated to some extent by late-log-phase cultures added to suspensions of individual isomers and incubated for a further 3 h. Of the cis-isomers, δ5 to δll (79–73% conversion to stearic acid) were the preferred substrates. δ24s- (30%) and δ13-cis-isomers ( 5 % ) were poorly hydrogenated. Of the trans-isomers, δ8, δ9 and δ10 were 45% converted to stearic acid, the other isomers were poorly hydrogenated. These results are in agreement with less extensive studies using sheep rumen micro-organisms.

3. When cultures were grown from small inocula in media containing individual isomers more extensive hydrogenation was found than with late-log-phase cultures. At 24 h, cis δ2, δ4 and δ5 gave the highest conversions to stearic acid (90%) followed by the cis δ6 to δ12 and trans δ8 to δ10 isomers (approximately 75%), although at 6 and 12 h δ9-trans gave higher yields of stearic acid than δ9-cis, probably because the growth of the cis cultures showed a longer log-phase.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 52 , Issue 1 , July 1984 , pp. 165 - 170
Copyright
Copyright © The Nutrition Society 1984

References

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