Alterations in glucose homoeostasis seem to occur in the postprandial state long before they appear in the fasted state(Reference Hanefeld and Temelkova-Kurktschiev1–Reference Ceriello and Colagiuri5). Under physiological conditions, following a meal, insulin released by the pancreas stimulates glucose uptake by peripheral tissues, but this effect is mediated to a large extent by concomitant activation of the hepatic parasympathetic nerves(Reference Lautt, Macedo and Sadri6–Reference Puschel8), as demonstrated by the observations that hepatic parasympathetic denervation(Reference Xie and Lautt9, Reference Moore, Satake and Baranowski10) causes a major reduction (up to 60 %) in postprandial insulin sensitivity (IS)(Reference Xie and Lautt9), with the same effect being observed after atropine administration(Reference Xie and Lautt9, Reference Sadri, Reid and Afonso11). In fact, atropine administration does not produce any additional impairment of IS in previously hepatic parasympathetic denervated rats(Reference Xie and Lautt9, Reference Xie and Lautt12). Atropine's effects are dose dependent, and the same effect on IS can be achieved by intraportal and intravenous (i.v.) atropine administrations, although with different median effective doses (ED50), but without any changes in plasma concentrations of glucagon or insulin(Reference Takayama, Legare and Lautt13, Reference Reid, Latour and Legare14). Both hepatic denervation and atropine-induced insulin resistance can be reversed by intraportal acetylcholine administration(Reference Moore, Satake and Baranowski10, Reference Xie and Lautt15), suggesting that both procedures interfere with the same pathway and that the activation of hepatic parasympathetic nerves leads to acetylcholine release in the liver(Reference Puschel8, Reference Xie and Lautt15, Reference Guarino, Correia and Lautt16). Acetylcholine stimulates muscarinic receptors(Reference Xie and Lautt15) and results in hepatic NO production(Reference Guarino, Correia and Lautt16, Reference Sadri and Lautt17). As with acetylcholine(Reference Moore, Satake and Baranowski10, Reference Xie and Lautt15), administration of a NO donor to a denervated liver(Reference Sadri and Lautt17) also restores whole-body postprandial IS.
This hepatic parasympathetic-dependent NO mechanism is additionally dependent upon the availability of hepatic glutathione, which is increased after a meal, when hepatic pathway activation is also maximal(Reference Guarino, Afonso and Raimundo18). This hepatic-dependent mechanism seems to result in the hepatic release of a humoral factor, referred to as hepatic insulin-sensitising substance (HISS), which acts mainly in the skeletal muscle(Reference Xie and Lautt9) and appears to be responsible for 50–60 % of whole-body insulin action in the postprandial state(Reference Lautt, Macedo and Sadri6, Reference Xie and Lautt9, Reference Sadri, Reid and Afonso11, Reference Guarino, Afonso and Raimundo18, Reference Lautt19). This mechanism accounts for the increment in IS from the fasted to the fed state(Reference Guarino and Macedo20).
The HISS-dependent contribution to overall postprandial insulin action can be calculated from the difference in postprandial IS before and after the inhibition of the HISS pathway, which can be achieved by either fasting(Reference Lautt, Macedo and Sadri6, Reference Sadri, Reid and Afonso11), parasympathetic denervation(Reference Xie and Lautt9, Reference Xie and Lautt15), blockade with atropine(Reference Xie and Lautt15) or hepatic NO synthase inhibition (e.g. using NG-monomethyl-l-arginine or NG-nitro arginine methyl ester)(Reference Sadri and Lautt17, Reference Guarino, Afonso and Raimundo18, Reference Afonso, Ribeiro and Fernandes21). Similar impairment of insulin action can be obtained by hepatic glutathione depletion(Reference Guarino, Afonso and Raimundo18). Autonomic dysfunction is observed in obesity(Reference Peterson, Rothschild and Weinberg22–Reference Lindmark, Lonn and Wiklund24), suggesting that decreased parasympathetic activity is associated with increased body fat(Reference Teff and Townsend7, Reference Peterson, Rothschild and Weinberg22, Reference Lindmark, Lonn and Wiklund24).
Earlier experiments that were performed by our group, in which only IS was assessed, suggest that short-term high-fat diet (HFD, 1 week) induces partial impairment of postprandial IS(Reference Afonso, Lautt and Ribeiro25); however, to what extent and the time course during which HFD induces the deterioration of postprandial IS are not known, and neither the relationship between adiposity and postprandial IS or hepatic glutathione content is known. In the present study, we aimed to evaluate the effects of different HFD durations on fat deposition and obesity, as well as to relate them with the postprandial IS, which depends on HISS action. For the first time, we have aimed to study the relationship between abdominal and whole-body adiposities and HISS action.
Thus, in the present work, we have hypothesised that a HFD induces postprandial insulin resistance, which is accounted for by the decrease of HISS-dependent insulin action and is proportional to the degree of obesity. We tested this by measuring the effect of muscarinic nerve blockade on IS in rats fed a standard diet (STD), short-term (1 week) HFD or long-term (4 weeks) HFD. Fat mass deposition and its correlation with HISS-dependent and HISS-independent insulin resistance were evaluated. The present work reveals further pathways interconnecting the impact of diets on insulin resistance.
Experimental methods
Animals
Male Sprague–Dawley rats (Charles River Laboratories, Saint Constant, QC, Canada) were used. The rats reached our facilities at the age of 4 weeks, and were housed under climate-controlled conditions and a 12 h light–dark cycle (07.00–19.00 hours). During the conditioning period, rats were fed ad libitum with a standard laboratory chow (Prolab RMH 3000 5P00; Labdiet, St Louis, MO, USA), and they had free access to tap water.
At the end of the experiment, rats were killed by i.v. administration of an overdose of anaesthetic (sodium pentobarbital).
All applicable institutional and national guidelines for the care and use of animals were followed, and all the experimental procedures involving animals were approved by the University of Manitoba Ethics Committee on Animal Care. These studies were compliant with the NIH Principles of Laboratory Animal Care (NIH publication no. 85-23, revised 1985), the Canadian Council on Animal Care guidelines and the Laboratory Animal Care Guidelines of the European Union (86/609/CEE).
Diets and feeding protocols
The control diet that was used was the standard laboratory chow (STD), which consisted of 26 % protein, 60 % carbohydrates and 14 % fat, whereas the HFD (DIO Series Diets D12492; Research Diet, Inc., New Brunswick, NJ, USA) consisted of 20 % protein, 20 % carbohydrates and 60 % fat (percentage of total energy) as described previously(Reference DeFuria, Bennett and Strissel26); the fat content was mostly provided by soyabean oil and lard (5·5 and 54·4 % of the total energy, respectively). After 1 week of conditioning, the rats were randomly divided into three groups according to the diet they were fed: the control group (n 14), which was fed the standard laboratory diet for the whole period of the study; the HFD-1 group (n 9), which was fed HFD for 1 week, between 8 and 9 weeks of age; and the HFD-4 group (HFD-4, n 10), which was fed HFD for 4 weeks, between 5 and 9 weeks of age.
All the rats were tested at the age of 9 weeks. They were given free access to food and water. On the day before the experiment, rats were fasted for 8 h, starting from 22.00 hours. Free access to water was maintained. At 06.00 hours on the next day, rats were allowed access to food for 2 h (HFD for the HFD-1 (n 9) and HFD-4 (n 7) groups; STD for the STD group (n 10)) in order to ensure that they had eaten by the time the experiment was started (postprandial studies).
A small group of STD-fed (n 4) and HFD-4-fed (n 3) rats were subjected to a 24 h fast, which was started at 08.00 hours on the day before the experiment in order to assess IS in the fasted state.
Surgical procedure
The rats were anaesthetised using sodium pentobarbital (65 mg/kg, intraperitoneal). Body temperature was maintained at 37·0 ± 0·5°C throughout the experiment using a Homeothermic Blanket Control Unit (Harvard Apparatus, Holliston, MA, USA).
A tracheotomy was performed to allow spontaneous respiration. Anaesthesia was maintained by continuous infusion of sodium pentobarbital (10 mg/h per kg, i.v.) through a left jugular vein catheter (polyethylene tubing PE50, Intramedic; Becton Dickinson, Franklin Lakes, NJ, USA), which was also used to infuse glucose.
The right femoral artery and vein were also catheterised. A femoral arterial-venous shunt was created by connecting the catheters through a silicone sleeve, which allows blood circulation(Reference Lautt, Wang and Sadri27). The arterial-venous shunt was primed with sodium heparin (200 IU/ml) to prevent blood clotting. Mean arterial pressure was obtained by clamping the venous side of the shunt, and was monitored using the Lab View Software (National Instruments, Austin, TX, USA).
Multiple arterial blood samples were collected by puncturing into the arterial side, and i.v. drug infusions were given through the venous side of the shunt. After the surgery, a 30 min period (minimum) was allowed for the measurement of blood glucose and blood pressure before further procedures were undertaken.
Insulin sensitivity assessment
The rapid IS test (RIST) was used to assess IS as described previously(Reference Lautt, Wang and Sadri27). The RIST is a modified euglycaemic clamp procedure, which is suitable for the rapid and reproducible assessment of IS. Since there is no carry-over effect between RIST, it is possible to repeat the RIST up to four times in the same animal on the same day(Reference Lautt, Wang and Sadri27), providing a suitable index for IS both in anaesthetised and conscious animals(Reference Sadri, Reid and Afonso11, Reference Latour and Lautt28). The RIST also allows for the assessment of IS in both the fasted and the fed states(Reference Lautt, Macedo and Sadri6).
Before each RIST, basal arterial glycaemia (baseline) was determined. The RIST was started with the administration of an i.v. bolus of insulin (50 mU/kg, 5 min; t = 0 min), and 1 min later (t = 1 min), glucose infusion (i.v.) was given (at a rate of 3 mg/kg per min). Then, at 2-min intervals, arterial glycaemia was measured, and the glucose infusion rate was adjusted (every 2 min) accordingly (using a ‘Genie’ infusion pump; Kent Scientific Corporation, Torrington, CT, USA) in order to maintain euglycaemia. The RIST was considered complete when no further glucose infusion was required to maintain euglycaemia. The total amount of glucose infused during the RIST is referred to as the RIST index (mg glucose per kg body weight, bw), and it is the parameter used to evaluate IS.
Glycaemia was determined by the glucose oxidase method using a glucose analyser (1500 Sport Analyser; Yellow Springs Instruments, Yellow Springs, OH, USA).
Insulinaemia quantification
Before and after each RIST, arterial blood samples (40 μl) were collected and centrifuged (3 min, 16 755 g; Corning Costar Corporation centrifuge, Cambridge, MA, USA) to determine plasma insulin levels, which was done using an ELISA technique (Rat Ultrasensitive Insulin ELISA; Mercodi, Inc., Winston Salem, NC, USA).
Hepatic glutathione quantification
At the end of the experiment, before the rats were killed, liver samples were collected and immediately frozen ( − 70°C) for spectrophotometric quantification of glutathione using the Bioxytech GSH-420 kit (from Oxisresearch, Portland, OR, USA).
Fat mass assessment
Fat mass assessment was done by measuring both whole-body fat mass and the weights of specific fat pads. Whole-body fat mass was estimated by tetrapolar bioelectrical impedance analysis using a Bioelectrical Body Composition Analyser (Quantum II; RJL Systems, Clinton Township, MI, USA) as described previously(Reference Hall, Lukaski and Marchello29).
At the end of the experiment (fed rats), a laparotomy was performed in order to harvest the abdominal fat pads, i.e. the fat pads surrounding the kidneys (perinephric), the testis (epididymal) and the intestine (perienteric). Total abdominal fat pad mass was the sum of the individual weights of perinephric, epididymal and perienteric fat pads.
Experimental protocols
An initial postprandial stable arterial glycaemic baseline (control baseline) was determined, and a control RIST was performed. Atropine (1 mg/kg, 5 min, i.v.) was then infused to block the HISS pathway. A 30 min period was then allowed for stabilisation before a new glycaemic baseline (post-atropine baseline) was determined, and another RIST (post-atropine RIST) was performed. The HISS-dependent component of insulin action was quantified by subtracting the post-atropine RIST index from the control RIST index.
In the STD-fed and HFD-fed rats that were to be tested in the fasted state (24 h fast), a single RIST was performed, following the determination of fasted glycaemic baseline.
Before and after each RIST (protocols followed in the fed state), arterial blood samples were collected for the determination of plasma insulin levels as described previously. At the end of the experiment, liver samples were collected for glutathione quantification (see above).
Statistical analysis
Data are presented as means with their standard errors. The significance of the differences was calculated through two-tailed Student's t tests and one-way ANOVA followed by the Tukey–Kramer multiple comparison tests, repeated-measures ANOVA or through ANCOVA, as applicable. Differences were accepted as statistically significant at P < 0·05.
Regression analysis (linear and polynomial) was used to assess the relationship between insulin action (total, HISS-dependent and HISS-independent) and fat mass, both whole body and regional (perinephric, epididymal and perienteric pads), using Stata version 9.2 (StataCorp, College Station, TX, USA).
Drugs
Sodium pentobarbital (Somnotol) was obtained from Biomeda-MTC Animal Healt, Inc., Cambridge, ON, Canada; sodium heparin was obtained from Leo Pharma, Thornhill, ON, Canada; insulin (Novolin) was obtained from Novo Nordisk, Mississauga, ON, Canada; and d-glucose and atropine were supplied by Sigma-Aldrich, Oakville, ON, Canada. All solutions were prepared in saline (Baxter Corporation, Toronto, ON, Canada).
Results
Body weight and blood pressure
A relationship between bw and HFD duration was observed: bw of the STD-fed rats (STD, 371·6 (se 7·3) g, n 10) was lower than those of the 1-week HFD-fed rats (HFD-1, 401·4 (se 5·2) g, n 9; P < 0·001) and the 4-week HFD-fed rats (HFD-4, 420·5 (se 4·7) g, n 7; P < 0·001).
Basal mean arterial pressure was similar in all the groups (STD, 116·6 (se 5·4) mmHg; HFD-1, 115·0 (se 5·6) mmHg; HFD-4, 115·0 (se 5·8) mmHg), and was unaltered throughout the experiment (post-atropine: STD, 118·0 (se 6·6) mmHg; HFD-1, 110·7 (se 5·2) mmHg; HFD-4, 113·8 (se 11·4) mmHg).
Glycaemia
There was no difference in the basal postprandial arterial glucose levels between the HFD-1-fed (7·1 (se 0·4) mmol/l, n 9), HFD-4-fed (7·6 (se 0·8) mmol/l, n 7) and STD-fed (6·5 (se 0·1) mmol/l, n 10) rats, although the glucose levels tended to be higher in the HFD-fed rats. These glycaemic levels were not significantly affected either by the control RIST (STD, 6·5 (se 0·1) mmol/l; HFD-1, 7·0 (se 0·4) mmol/l; HFD-4, 7·8 (se 0·8) mmol/l) or by atropine administration (STD, 6·5 (se 0·1) mmol/l; HFD-1, 7·1 (se 0·3) mmol/l; HFD-4, 7·8 (se 1·0) mmol/l).
The fasting (24 h fast) arterial glycaemia was similar between the STD-fed (5·2 (se 0·2) mmol/l, n 4) and HFD-4-fed (5·7 (se 0·2) mmol/l, n 3) rats, despite it showing a tendency to increase with the HFD feeding.
Insulinaemia
Basal postprandial insulinaemia was not statistically different between the STD-fed (2·3 (se 0·4) μg/l) and HFD-1-fed (4·2 (se 0·5) μg/l) rats, but it was higher in the HFD-4-fed rats (4·6 (se 0·7) μg/l; P < 0·05 v. STD). After the control RIST, insulin levels remained at baseline (STD, 2·4 (se 0·4) μg/l; HFD-1, 4·6 (se 0·3) μg/l; HFD-4, 6·1 (se 1·2) μg/l). Atropine administration did not affect insulinaemia (STD, 1·9 (se 0·4) μg/l; HFD-1, 6·0 (se 0·9) μg/l; HFD-4, 4·9 (se 0·7) μg/l), and the post-atropine RIST insulin levels were also similar to the baseline levels (STD, 2·3 (se 0·4) μg/l; HFD-1, 5·4 (se 1·2) μg/l; HFD-4, 4·9 (se 0·8) μg/l).
Hepatic glutathione levels
Hepatic glutathione levels were different between the STD-fed (6·2 (se 0·1) μmol/g, n 10) and HFD-fed (HFD-1, 5·1 (se 0·2) μmol/g, n 8; P < 0·01 v. STD; HFD-4, 5·4 (se 0·3) μmol/g, n 7; P < 0·05 v. STD) rats, although there was no difference between the HFD-1-fed and HFD-4-fed rats.
Insulin sensitivity assessment
Fasting IS, measured after a 24 h fast, in which HISS contribution is absent, was not different between STD-fed (80·2 (se 5·4) mg glucose/kg bw, n 4) and HFD-4-fed (79·0 (se 6·0) mg glucose/kg bw, n 3) groups, suggesting that the HFD does not induce changes in the HISS-independent component of insulin action (insulin action per se).
Postprandial insulin action comprises both the HISS-dependent and HISS-independent components. As indicated by the control RIST index (Fig. 1(a)), postprandial IS was lower in the HFD-4-fed rats (69·3 (se 2·6) mg glucose/kg bw, n 7) than in both the HFD-1-fed (108·8 (se 2·9) mg glucose/kg bw, n 9; P < 0·001) and STD-fed (185·8 (se 5·6) mg glucose/kg bw, n 10; P < 0·001) rats, suggesting that insulin resistance increases with HFD duration. The difference in insulin action between the HFD-1-fed and STD-fed rats was also statistically significant (P < 0·001).
Fig. 1 Postprandial insulin sensitivity, assessed by the rapid insulin sensitivity test (RIST), was significantly lower in both the 1-week high-fat diet-fed (HFD-1, n 9) and 4-week HFD-fed (HFD-4, n 7) rats than in the control rats (standard diet (STD), n 10), an effect which seems to be primarily due to the impairment of the hepatic insulin-sensitising substance (HISS) pathway-dependent component. (a) RIST index for the HISS-dependent (□) and HISS-independent (post-atropine, ■) components of insulin action. The HISS-independent component represents the direct insulin action. The sum of both components represents the control RIST index (postprandial insulin action) for each group. (b) Relative contribution (%) of the HISS-dependent component to the overall postprandial insulin action in the STD-fed (□), HFD-1-fed (□) and HFD-4-fed (■) rats. Data are means with their standard errors. *** Mean values were significantly different (P < 0·001). bw, Body weight.
The insulin action observed after the blockade of the hepatic parasympathetic nerves (post-atropine RIST index), i.e. after completely abolishing the HISS component, corresponded to the HISS-independent insulin action (Fig. 1(a)). The HISS-independent (post-atropine) insulin action was similar between the STD-fed (76·9 (se 3·8) mg glucose/kg bw, n 10) and HFD-1-fed (70·2 (se 2·9) mg glucose/kg bw, n 9) rats, but it was slightly lower in the HFD-4-fed rats (63·8 (se 1·8) mg glucose/kg bw, n 7; P < 0·05 v. STD only).
In contrast, the HISS-dependent component of insulin action (Fig. 1(a)), obtained by subtraction of the post-atropine RIST index from the control RIST index, decreased significantly with HFD duration: STD, 108·9 (se 3·9) mg glucose/kg bw; HFD-1, 38·6 (se 4·2) mg glucose/kg bw (P < 0·001 v. STD); HFD-4, 5·4 (se 1·7) mg glucose/kg bw (P < 0·001 v. STD and HFD-1).
HISS pathway contribution to overall insulin action (Fig. 1(b)) was reduced in the HFD-1-fed rats (37·1 (se 3·4) %), and became relatively insignificant in the HFD-4-fed rats (7·5 (se 2·2) %; P < 0·001 v. HFD-1) than in the STD-fed rats (58·7 (se 1·5) %; P < 0·001 v. HFD-4 and HFD-1).
HISS-dependent insulin action was decreased by 64·6 % in the HFD-1-fed rats than in the STD-fed rats, increasing to 95 % suppression after 4 weeks of HFD feeding. In contrast, the HISS-independent component was less affected, since the fall was only significant in the HFD-4-fed rats, in which the HISS-independent insulin action was 17 % lower than that in the STD-fed rats. HFD seems to affect the HISS pathway first, and that too to a greater extent than the HISS-independent component.
Fat mass assessment
Whole-body fat mass, determined by tetrapolar bioelectrical impedance, was significantly higher in the HFD-4-fed rats (n 7) than in the HFD-1-fed (P < 0·05) and STD-fed (n 9; P < 0·001 v. HFD-1 and HFD-4) rats (Table 1). The non-fat mass was similar between the HFD-4-fed (314·1 (se 8·0) g) and HFD-1-fed (323·0 (se 6·9) g) rats, but it was higher in the STD-fed rats (347·4 (se 7·5) g; P < 0·05 v. HFD-4). In terms of bw percentage, whole-body fat mass was 25·5 (se 1·5) % in the HFD-4-fed rats, 19·7 (se 1·8) % in the HFD-1-fed rats (P < 0·05) and 8·5 (se 0·9) % in the STD rats (P < 0·001 v. HFD-1; P < 0·001 v. HFD-4).
Table 1 Whole-body fat, individual abdominal fat pads (perinephric, epididymal and perienteric) and total regional fat pad masses of rats fed the standard chow diet (STD) and of rats fed the high-fat diet (HFD) for 1 week (HFD-1) and 4 weeks (HFD-4)§
(Mean values with their standard errors)
* Mean values were significantly different compared with STD (P < 0·001).
† Mean values were significantly different compared with HFD-1 (P < 0·05).
‡ Mean values were significantly different compared with HFD-1 (P < 0·01).
§ Total fat pad mass was determined by the sum of perinephric, epididymal and perienteric fat pads masses.
There was a negative polynomial (square) correlation between whole-body fat mass, measured by bioimpedance, and overall postprandial insulin action, given by the control RIST index (R 2 0·81, n 16; P < 0·05) (Fig. 2). There was also a similar negative polynomial correlation between whole-body fat mass and HISS-dependent insulin action (R 2 0·87; P < 0·01) (Fig. 2). In contrast, there was no correlation between the HISS-independent insulin action (post-atropine RIST) and whole-body fat mass, either polynomial (R 2 0·20; P = 0·73) or linear (R 2 0·19; P = 0·09) (Fig. 2). These results suggest that the increase in whole-body adiposity affects the HISS-dependent component of insulin action (or vice versa) in a fast and pronounced way, whereas it is not associated with significant changes in the HISS-independent insulin action.
Fig. 2 There is a negative polynomial correlation between whole-body fat mass, measured by bioelectrical impedance, and insulin action. (a) Whole-body fat mass correlates (negative polynomial correlation) with total insulin action (R 2 0·81; P < 0·05). (b) There is also a negative polynomial correlation between hepatic insulin-sensitising substance (HISS) pathway-dependent insulin sensitivity and whole-body fat mass (●, R 2 0·87; P < 0·01); however, there is no correlation between whole-body fat mass and HISS-independent insulin action (▲; P = 0·73). bw, Body weight.
The individual weights of the abdominal fat depots collected (perinephric, epididymal and perienteric) increased significantly with HFD duration (Table 1). Total regional fat pad content, given by the sum of the individual fat pad weights, was also higher in the HFD-4-fed rats than in the HFD-1-fed rats (P < 0·01), which had higher content than the STD-fed rats (P < 0·001 v. HFD-1 and HFD-4) as shown in Table 1.
The weight of each of the abdominal fat depots collected (perirenal, perienteric and epididymal) was also negatively correlated with IS as shown in Table 2. Indeed, a negative linear correlation was observed between each of the individual fat pad masses and overall insulin action (control RIST index), HISS-dependent and HISS-independent insulin action (Table 2). Similarly, there was also a negative linear correlation between the sum of the individual abdominal fat pad masses (total regional fat) and total insulin action (R 2 0·85; P < 0·001), HISS-dependent insulin action (R 2 0·79; P < 0·001) and HISS-independent insulin action (R 2 0·45; P < 0·01) (Table 2 and Fig. 3). However, this correlation was stronger for the HISS-dependent component (adjusted R 2, 0·78) than for the HISS-independent component (adjusted R 2, 0·41; P < 0·001) of insulin action (Fig. 3). In Fig. 3, the slopes of the linear regressions for total ( − 5·4 (se 0·6)) and HISS-dependent insulin action ( − 4·6 (se 0·6)) are more pronounced than the slopes of the linear regressions for the HISS-independent insulin action ( − 0·9 (se 0·3)), similar to that observed for the individual abdominal fat depots (Table 2), suggesting that the change in regional adiposity has a more pronounced impact on the HISS-dependent component than on the HISS-independent component.
Table 2 Correlation of both the components of whole-body insulin action (hepatic insulin-sensitising substance (HISS)-dependent and HISS-independent) with perinephric, perienteric and epididymal fat masses
(Mean values with their standard errors and R 2 values)
bw, Body weight; R 2, squared R of the linear regressions.
* Mean values were significantly different from zero (P < 0·001).
† Mean values were significantly different from zero (P < 0·05).
‡ Mean values were significantly different from zero (P < 0·01).
§ Mean values were significantly different from HISS-independent action (P < 0·001).
Fig. 3 Total regional fat pad mass and insulin action present a negative linear correlation. (a) Total regional fat pad mass v. overall insulin action (R 2 0·85, adjusted R 2 0·84; P < 0·001). (b) Regional fat mass v. hepatic insulin-sensitising substance (HISS)-dependent (●, R 2 0·79, adjusted R 2 0·78; P < 0·001) and HISS-independent (▲, R 2 0·45, adjusted R 2 0·41; P < 0·01) insulin action. The total regional fat pad mass is given by the sum of the individual weights of the perinephric, perienteric (abdominal) and epididymal fat depots.
Discussion
In the present study, we observed that increasing HFD durations lead to increasing insulin resistance, specifically in the postprandial state, with less impact on fasting IS. Our data suggest that the major contribution to the HFD-induced postprandial insulin resistance derives from the significant impairment of HISS action, which correlates with adiposity.
Methodological considerations
Most methods used to assess IS are based solely on the steady-state glucose and insulin concentrations observed in the fasted state, disregarding postprandial insulin action. We used the transient euglycaemic clamp, RIST, to assess IS, since this is the most appropriate test for the present study, which requires more than one evaluation of IS in the postprandial state. Anaesthesia does not alter the outcome of the RIST(Reference Sadri, Reid and Afonso11), and insulin administered as a bolus does not induce vagal impairment, as observed for the methods that require insulin infusions that are to be given for a longer duration resulting in hyperinsulinaemic states(Reference Van De Borne, Hausberg and Hoffman30). The RIST is reproducible within the same experiment (up to four consecutive RIST) while retaining high sensitivity(Reference Afonso, Ribeiro and Fernandes21, Reference Lautt, Wang and Sadri27), and it has been used in several animal models(Reference Sadri, Reid and Afonso11, Reference Afonso, Ribeiro and Fernandes21, Reference Lautt, Wang and Sadri27, Reference Ribeiro, Lautt and Legare31, Reference Ribeiro, Afonso and Macedo32) and in human subjects(Reference Patarrao, Lautt and Guarino33), with results comparable to those obtained using the insulin tolerance test(Reference Reid, Latour and Legare14).
The use of atropine to specifically block the HISS-dependent component of insulin action has been validated already by numerous studies performed in several different models, showing no additional effects on insulin action per se or insulinaemia(Reference Lautt, Macedo and Sadri6, Reference Sadri, Reid and Afonso11, Reference Sadri and Lautt17, Reference Afonso, Ribeiro and Fernandes21, Reference Ribeiro, Afonso and Macedo32, Reference Ribeiro, Afonso and Guarino34, Reference Lautt, Ming and Macedo35). Moreover, the impact on postprandial IS by the use of atropine equals that observed after selective hepatic parasympathetic surgical ablation(Reference Sadri, Reid and Afonso11, Reference Xie and Lautt15). The fasted-state experiments further provided an estimation of the efficacy of the blockade of parasympathetic nerves by atropine.
Our aim was to compare a regular STD with a diet that was clearly rich in fat content (HFD). We used a 60 % HFD (energetic contribution of fat to the diet) in order to detect changes induced by dietary fat in a short period of time. This was done to avoid having ageing as a confounding factor for the present study, since we reported previously that ageing affects HISS-dependent insulin action(Reference Ribeiro, Afonso and Guarino34).
The effect of high-fat diet on hepatic glutathione
Reports from several groups suggest that HFD induces oxidative stress(Reference Roberts, Barnard and Sindhu36–Reference Milagro, Campion and Martinez38) in a process that may involve glutathione depletion(Reference Carmiel-Haggai, Cederbaum and Nieto37). Whether the oxidative stress caused by an HFD-induced depletion of glutathione is directly linked to the impairment of IS remains unclear. We determined glutathione content in the liver, since a decrease in hepatic glutathione could lead not only to a state of reduced free radical quenching, but also to a compromise in postprandial insulin action as glutathione is required for HISS-dependent insulin action(Reference Guarino, Afonso and Raimundo18).
The hepatic glutathione levels were higher in the STD-fed rats than in the HFD-fed rats, but there was no difference between the HFD-1- and HFD-4-fed rats, although postprandial insulin resistance was aggravated during these periods. These results suggest that glutathione impairment might not be directly involved in the progressive increase of insulin resistance with HFD duration, although it might play a role in the initiation of the process.
The effect of high-fat diet on insulin sensitivity
The HFD induced postprandial insulin resistance, which was already present after 1 week (HFD-1: approximately 59 % of the IS in the STD-fed rats) and was further aggravated by 4 weeks on HFD feeding (approximately 37 % of the IS observed in the STD-fed rats), in accordance with the previous observations(Reference Zhang, Ye and Li39–Reference Pagliassotti, Gayles and Podolin41).
Postprandial insulin action depends not only on insulin per se, but also on a hepatic mechanism that involves both parasympathetic nerves(Reference Xie and Lautt15) and glutathione(Reference Guarino, Afonso and Raimundo18) – the HISS-dependent component of insulin action(Reference Guarino and Macedo20).
HISS-independent insulin action was unaltered during the HFD-1 feeding, and it was only slightly decreased in the HFD-4-fed group. This observation possibly explains the fact that not all authors observe differences in insulin-induced glucose utilisation between HFD-fed and control animals(Reference Cruciani-Guglielmacci, Vincent-Lamon and Rouch42), although a majority do(Reference Zhang, Ye and Li39–Reference Pagliassotti, Gayles and Podolin41). Such differences could arise from variations in diet composition, diet duration and the methodology used to assess IS. HFD feeding that is carried out for a period longer than 4 weeks may also lead to the deterioration of the HISS-independent component of insulin action, and result in fasting hyperglycaemia or frank diabetes.
The reduction in HISS-dependent insulin action paralleled the reduction in total postprandial IS: an impairment of about 65 % was observed after 1 week of HFD feeding, and by 4 weeks of HFD feeding, HISS action was completely absent. Since 1 week of HFD feeding was not sufficient to affect HISS-independent insulin action, HISS impairment seems to be primarily responsible for the early postprandial insulin resistance associated with HFD.
Because there were no differences in hepatic glutathione levels between the HFD-1-fed and HFD-4-fed rats, the progressive decrease of HISS action appears to be solely due to the impairment of hepatic parasympathetic nerves.
The postprandial insulin resistance described for the HFD-induced obesity model studied herein is mechanistically different from that observed in other animal models of obesity, such as the genetically obese Zucker (fa/fa) rats, in which the two components of insulin action were impaired in the same proportion(Reference Afonso, Ribeiro and Fernandes21), probably due to post-insulin receptor abnormalities at the level of the skeletal muscle(Reference Brozinick, Etgen and Yaspelkis43, Reference King, Horton and Hirshman44). This does not seem to be the case with HFD-induced model, in which insulin resistance was observed only in the postprandial state for the period studied, which was caused by impairment of HISS action.
High-fat diet-induced obesity, parasympathetic dysfunction and postprandial insulin resistance
The importance of hepatic parasympathetic nerves in insulin-induced glucose homoeostasis has been addressed previously(Reference Lautt, Macedo and Sadri6, Reference Sadri, Reid and Afonso11, Reference Xie and Lautt15, Reference Xie and Lautt45, Reference Stumpel, Scholtka and Jungermann46). Work done by others has shown functional decrease of intra-hepatic muscarinic nerves in livers of streptozotocin-treated rats, suggesting that impairment of hepatic parasympathetics can deprive the chronically diabetic patient of an adequate response to postprandial hyperglycaemia(Reference Stumpel, Scholtka and Jungermann46). More recently, studies performed by Li et al. (Reference Li, Gautam and Han47) in mice suggest that the hepatic muscarinic acetylcholine receptors are not essential to ensure adequate IS in the fasted state. These findings are in agreement with our previous data, showing that although hepatic parasympathetic nerves are not critical for fasting IS, they are required for proper postprandial IS as we reported previously(Reference Sadri, Reid and Afonso11). Furthermore, Li et al. (Reference Li, Gautam and Han47) did not observe any effect of overexpression of hepatic muscarinic receptors in the fasted state, which in the light of the HISS hypothesis should be expected, since besides parasympathetics, hepatic glutathione content must also increase, which is achieved by feeding(Reference Guarino, Afonso and Raimundo18).
Several studies have addressed the association between obesity and parasympathetic dysfunction(Reference Teff and Townsend7, Reference Peterson, Rothschild and Weinberg22, Reference Lindmark, Lonn and Wiklund24), and although no tissue specificity of such parasympathetic dysfunctions has been reported, the liver seems to be affected(Reference Teff and Townsend7). A hepatic parasympathetic dysfunction associated with obesity could result in the impairment of the HISS pathway and, consequently, of the postprandial insulin action.
Obesity has been associated with an imbalance between sympathetic and parasympathetic nervous systems caused by increased sympathetic tone(Reference Lindmark, Lonn and Wiklund24, Reference Landsberg48), therefore leading to the impairment of parasympathetic activity(Reference Lindmark, Lonn and Wiklund24, Reference Van Vliet, Hall and Mizelle49). The existence of an association between the parasympathetic nervous system, visceral fat and liver, proposed by Fliers et al. (Reference Fliers, Kreier and Voshol50), together with the observations suggesting that attenuation of parasympathetic activity promotes insulin resistance(Reference Moore, Satake and Baranowski10, Reference Sadri, Reid and Afonso11, Reference Xie and Lautt15, Reference Afonso, Ribeiro and Fernandes21, Reference Ribeiro, Afonso and Macedo32, Reference Fliers, Kreier and Voshol50–Reference Bartness53), provides further support for our conclusions. In addition to hepatic parasympathetic nerves, acetylcholine and NO are also intermediates in the HISS pathway, and functional impairment of either can result in HISS-dependent insulin resistance(Reference Xie and Lautt15–Reference Guarino, Afonso and Raimundo18, Reference Afonso, Ribeiro and Fernandes21). Therefore, recent reports of impaired acetylcholine-induced activation of NO synthase(Reference Zecchin, Priviero and Souza54) and NO-related abnormalities in HFD-induced obese models(Reference Du, Edelstein and Obici55, Reference Erdei, Toth and Pasztor56) are also consistent with our data.
Fat mass and insulin resistance
The decrease in postprandial IS observed in the HFD-fed rats was associated with the increase in adipose mass as in previous reports(Reference Milagro, Campion and Martinez38, Reference Pitombo, Araujo and De Souza57–Reference Lim, Son and Song59). The present results add to this by showing that the effect of adiposity is predominantly mediated by the abrupt decrease in the HISS-dependent component of insulin action. Indeed, there was a significant polynomial correlation between whole-body fat mass and HISS-dependent insulin action, which was similar to the correlation between whole-body fat mass and total insulin action, whereas the HISS-independent insulin action did not correlate with the whole-body fat mass (Fig. 2). On the other hand, the regional abdominal fat mass presents a linear correlation with both components of insulin action, although this correlation was stronger for the total and for HISS-dependent insulin action than for the HISS-independent insulin action, as given by the adjusted R 2 and linear regression slopes (Table 2 and Fig. 3). This suggests either that both the HISS-dependent and HISS-independent components of insulin action decrease with adiposity, or that the fat accumulation results from the observed insulin resistance. Nevertheless, we also showed that the HISS-independent insulin action (insulin action per se) tends to decrease at a slower rate than the HISS-dependent action.
In the present study, we evaluated fat content by measuring both whole-body fat and specific regional fat pad masses. The measurements of whole-body fat and specific abdominal fat depots, which provided us with an assessment of the fat accumulation in adipose tissue in response to the HFD, were shown for the first time to be associated with pronounced insulin resistance due to HISS pathway impairment. These results are in agreement with the previous reports associating high adiposity with severe insulin resistance, both in human subjects(Reference Lindmark, Lonn and Wiklund24) and in animal models(Reference Pagliassotti, Gayles and Podolin41, Reference Barzilai, She and Liu58–Reference Korach-Andre, Gao and Gounarides60). Despite this strong correlation, we cannot differentiate cause and effect, i.e. whether the inadequate HISS-dependent insulin action causes the high-fat storage and concomitant adiposity, or vice versa. However, the HISS hypothesis proposes that the absence of HISS action shifts the balance of nutrient storage away from glycogen in skeletal muscle towards fat deposition.
Studies performed by other groups support the involvement of visceral and/or abdominal fat in the process of obesity-related insulin resistance(Reference Lindmark, Lonn and Wiklund24, Reference Pitombo, Araujo and De Souza57), and report that visceral fat removal improves IS in animal models of obesity(Reference Pitombo, Araujo and De Souza57, Reference Barzilai, She and Liu58). However, the increase in regional abdominal fat pad mass and that in whole-body fat mass were correlated, suggesting that HFD does not induce fat accumulation in any specific site, but rather induces a general increase in adiposity, i.e. whole-body fat, which is similar to the observations reported by others(Reference Lim, Son and Song59, Reference Korach-Andre, Gao and Gounarides60). Therefore, the development of insulin resistance due to an inadequate HISS pathway action does not seem to be associated with fat deposition in any particular depot.
In conclusion, HISS pathway is essential for postprandial insulin action. The present study shows that impairment of HISS action is the major contributor for HFD-induced insulin resistance. It was observed for the first time that animals fed an HFD present increasing disturbances of glucose homoeostasis observed primarily and more severely in the postprandial state than in the fasted state: HFD resulted in insulin resistance, higher insulinaemia and slightly higher (NS) glycaemia in the postprandial state readily after 1 week of HFD feeding. Furthermore, the HISS impairment correlated with adiposity, as it did with the overall postprandial insulin resistance. In the HFD-4-fed group, fasted rats did not show significant alterations in insulin responsiveness.
Thus, our data suggest that HFD-induced obesity leads to a progressive decline in HISS-dependent IS, resulting in postprandial insulin resistance and predisposing to frank diabetes.
Acknowledgements
We are indebted to Dr Zhi Ming for sharing technical expertise and Gerald Nolette from the Central Animal Care Services of the University of Manitoba for animal care assistance. The present work was supported by the Portuguese Foundation for Science and Technology (FCT, grant POCI/SAU-OBS/56 716/2004), the Portuguese Society of Endocrinology, Diabetes and Metabolism and the Canadian Diabetes Association and Canadian Institutes of Health Research (operating grants). M. P. M. and W. W. L. were involved in the conceptual work; R. A. A. was involved in all aspects of the work presented; D. J. L. and J. S. conducted insulin and GSH quantifications; A. G. O. was involved in data analysis. There are no conflicts of interests to report.
