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Hepatic fatty acid metabolism in rats fed diets with different contents of C18:0, C18:1cis and C18:1trans isomers

Published online by Cambridge University Press:  09 March 2007

Anna M. Giudetti ,
Anton C. Beynen ,
Arnoldina G. Lemmens ,
Gabriele V. Gnoni  and
Math J. H. Geelen
Anna M. Giudetti
Affiliation:
Laboratory of Biochemistry, Department of Biological and Environmental Sciences and Technologies, University of Lecce, Lecce, Italy
Anton C. Beynen
Affiliation:
Department of Nutrition, PO Box 80.152, 3508 TD, Utrecht, The Netherlands
Arnoldina G. Lemmens
Affiliation:
Department of Laboratory Animal Science, Utrecht, The Netherlands
Gabriele V. Gnoni
Affiliation:
Laboratory of Biochemistry, Department of Biological and Environmental Sciences and Technologies, University of Lecce, Lecce, Italy
Math J. H. Geelen*
Affiliation:
Department of Nutrition, PO Box 80.152, 3508 TD, Utrecht, The Netherlands Laboratory of Veterinary Biochemistry, Graduate School of Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
*
*Corresponding author: Dr Math J. H. Geelen, fax +31 302 531 817, email [email protected]
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Abstract

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In the present study the effects of some C18 fatty acids on hepatic fatty acid metabolism have been compared. Male rats were fed cholesterol-free diets containing either C18:0, C18:1cis or C18:1trans isomers as the variables. In accordance with previous work, oleic acid in the diet caused an increase in cholesterol concentration in the liver and in the lipoprotein fraction of density (d; kg/l)<1·006. Oleic acid also reduced the triacylglycerol:cholesterol value in this fraction. Surprisingly, the C18:1trans isomers diet induced a decrease in the amount of cholesterol in total plasma as well as in the 1·019<d<1·063 lipoprotein fraction. Both oleic acid and C18:1trans isomers increased the concentration of triacylglycerols in the liver. The two C18:1 fatty acids differently influenced the hepatic activities of carnitine palmitoyltransferase-I and 3-hydroxy-acyl-CoA dehydrogenase; both enzymes were inhibited by C18:1trans isomers, while no change was induced by oleic acid. The activity of the citrate carrier was lower in the oleic acid- and C18:1trans isomers-fed rats, when compared with the rats fed stearic acid. No diet effects were seen for the activities of acetyl-CoA carboxylase, fatty acid synthase, diacylglycerol acyltransferase, citrate synthase and phosphofructokinase. The results are interpreted in that oleic acid raised liver triacylglycerol by reducing the secretion of it with the d<1·006 lipoprotein fraction whereas the C18:1trans isomers enhanced liver triacylglycerol by lowering the hepatic oxidation of fatty acids.

Keywords

Dietary fatty acidsHepatic enzymesLiverPlasma lipoproteins
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 5 , November 2003 , pp. 887 - 893
Copyright
Copyright © The Nutrition Society 2003

References

Bailey, NTJ (1995) Statistical Methods in Biology, 3rd ed., Cambridge, UK: Cambridge University Press.CrossRefGoogle Scholar
Beynen, AC (1988) Dietary monounsaturated fatty acids and liver cholesterol. Artery 15, 170–175.Google ScholarPubMed
Beynen, AC, Bogaard, A, Van Laack, HLJM & Katan, MB (1984) Cholesterol metabolism in two strains of rats with high or low responses to a cholesterol-rich diet. J Nutr 114, 1640–1651.Google Scholar
Bligh, EG & Dyer, WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37, 911–917.CrossRefGoogle ScholarPubMed
Fritz, IB, Cheema-Dhadli, S, Taylor, WM, Morris, HP & Halperin, ML (1973) Inhibition of hepatic fatty acid synthesis by long-chain fatty acyl-CoA derivatives: role of the mitochondrial citrate transporter. Alfred Benzon Symposium VI1, 645–652.Google Scholar
Geelen, MJH & Beynen, AC (2000) Consumption of olive oil has opposite effects on plasma total cholesterol and sphingomyelin concentrations in rats. Br J Nutr 83, 541–547.CrossRefGoogle ScholarPubMed
Geelen, MJH, Harris, RA, Beynen, AC & McCune, SA (1979) Short-term hormonal control of hepatic lipogenesis. Diabetes 29, 1006–1022.Google Scholar
Geelen, MJH, Schoots, WJ, Bijleveld, C & Beynen, AC (1995 a) Dietary medium-chain fatty acids raise and (n-3) polyunsaturated fatty acids lower hepatic triacylglycerol synthesis in rats. J Nutr 125, 2449–2456.Google ScholarPubMed
Geelen, MJH, Tijburg, LBM, Bouma, CJ & Beynen, AC (1995 b) Cholesterol consumption alters hepatic sphingomyelin metabolism in rats. J Nutr 125, 2294–2300.CrossRefGoogle ScholarPubMed
Geelen, SNJ, Blázquez, C, Geelen, MJH, Sloet Van Oldruitenborgh-Oosterbaan, MM & Beynen, AC (2001) High fat intake lowers hepatic fatty acid synthesis and raises fatty acid oxidation in aerobic muscle in Shetland ponies. Br J Nutr 86, 31–36.CrossRefGoogle ScholarPubMed
Groot, PH, de Boer, BC, Haddeman, E, Houtsmuller, UM & Hulsmann, WC (1988) Effect of dietary fat composition on the metabolism of triacylglycerol-rich plasma lipoproteins in the postprandial phase in meal-fed rats. J Lipid Res 29, 541–551.CrossRefGoogle Scholar
Guzmán, M, Klein, W, Gómez del Pulgar, T & Geelen, MJH (1999) Metabolism of trans fatty acids by hepatocytes. Lipids 34, 381–386.CrossRefGoogle ScholarPubMed
Guzmán, M, Kolodziej, MP, Caldwell, A, Costorphine, CG & Zammit, VA (1994) Evidence against direct involvement of phosphorylation in the activation of carnitine palmitoyltransferase by okadaic acid in rat hepatocytes. Biochem J 300, 693–699.CrossRefGoogle ScholarPubMed
Haagsman, HP, de Haas, CGM, Geelen, MJH, Van Golde, LMG (1982) Regulation of triacylglycerol synthesis in the liver. J Biol Chem 257, 10593–10598.Google Scholar
Hassid, WZ & Abraham, S (1957) Determination of glycogen. Methods Enzymol 3, 34–50.CrossRefGoogle Scholar
Katan, MB (1998) Health effects of trans fatty acids. Eur J Clin Invest 28, 257–258.CrossRefGoogle ScholarPubMed
Khosla, P & Hayes, KC (1996) Dietary trans -monounsaturated fatty acids negatively impact plasma lipids in humans: critical review of the evidence. J Am Coll Nutr 15, 325–339.CrossRefGoogle ScholarPubMed
Lawson, LD & Holman, RT (1981) Beta-oxidation of the geometric and positional isomers of octadecenoic acid by rat heart and liver mitochondria. Biochim Biophys Acta 665, 60–65.Google Scholar
Lowry, OH, Rosebrough, NJ, Farr, AL & Randall, RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193, 265–275.CrossRefGoogle ScholarPubMed
Metcalfe, LD, Schmitz, AA & Pelka, JR (1966) Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Anal Biochem 38, 514–515.Google Scholar
Nelson, GJ (1998) Dietary fat, trans fat, and risk of coronary heart disease. Nutr Rev 56, 250–252.CrossRefGoogle ScholarPubMed
Palmieri, F, Stipani, I, Quagliariello, E & Klingeberg, M (1972) Kinetic study of the tricarboxylate carrier in rat-liver mitochondria. Eur J Biochem 26, 587–594.CrossRefGoogle Scholar
Schiller, CM, Taylor, WM & Halperin, ML (1974) Control of fatty acid synthesis in white adipose tissue by insulin: coordination between the mitochondrial citrate transporter and pyruvate dehydrogenase. Can J Biochem 52, 813–821.CrossRefGoogle ScholarPubMed
Smits, CHM, Moughan, PJ & Beynen, AC (2000) The inhibitory effect of a highly viscous carboxymethylcellulose on dietary fat digestibility in the growing chicken is dependent on the type of fat. J Anim Physiol Anim Nutr 83, 231–238.CrossRefGoogle Scholar
Sundler, R, Åkesson, B & Nilsson, Å (1974) Effect of different fatty acids on glycerolipid synthesis in isolated rat hepatocytes. J Biol Chem 249, 5102–5107.Google Scholar
Ten Hoor, F, Rietveld, WJ, Kooij, M & Flory, W (1980) Growth and 24 hour eating patterns of rats kept under various light:dark conditions. Lab Anim 14, 251–252.Google Scholar
Terpstra, AHM, Woodward, CJH, Sanchez-Muniz, FJ (1981) Improved techniques for the separation of serum lipoproteins by density gradient ultracentrifugation: visualization by pre-staining and rapid separation of serum lipoproteins from small volumes of serum. Anal Biochem 111, 149–157.CrossRefGoogle Scholar
Tijburg, LBM, Maquedano, A, Bijleveld, C, Guzmán, M & Geelen, MJH (1988) Effects of ethanol feeding on hepatic lipid synthesis. Arch Biochem Biophys 267, 568–579.CrossRefGoogle ScholarPubMed
Verbeek, MJF, Van den Berg, GJ, Lemmens, AG & Beynen, AC (1993) High protein intake raises apparent but not true magnesium absorption in rats. J Nutr 123, 1880–1887.CrossRefGoogle Scholar
Williams, B (1993) Biostatistics. In Concepts and Applications for Biologists. London, UK: Chapman & Hall.Google Scholar
Woldseth, B, Retterstol, K & Christopherson, BO (1998) Monounsaturated trans fatty acids, elaidic acid and trans-vaccenic acid, metabolism and incorporation in phospholipid molecular species in hepatocytes. Scand J Clin Lab Invest 58, 635–645.CrossRefGoogle ScholarPubMed
Zara, V & Gnoni, GV (1995) Effect of starvation on the activity of the mitochondrial tricarboxylate carrier. Biochim Biophys Acta 1239, 33–38.Google Scholar