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Glutathione peroxidase (EC 1.11.1.9), glutathione-S-transferase (EC 2.5.1.13), superoxide dismutase (EC 1.15.1.1) and catalase (EC 1.11.1.6) activities in tissues of ducklings deprived of vitamin E and selenium

Published online by Cambridge University Press:  09 March 2007

Guang-Lu Xu  and
Anthony T. Diplock
Guang-Lu Xu
Affiliation:
Department of Biochemistry, Guy's Hospital Medical School, London SE1 9RT
Anthony T. Diplock
Affiliation:
Department of Biochemistry, Guy's Hospital Medical School, London SE1 9RT
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Abstract

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1. Vitamin E and selenium deficiencies were produced in the Pekin duckling (Anser cinerens) and were characterized by the development of lesions after 14 d in gizzard, intestine, heart and skeletal muscle.

2. The activities of glutathione peroxidase (EC 1.11.1.9), using hydrogen peroxide and cumene hydroperoxide as substrates, glutathione-S-transferase (EC 2.5.1.13), superoxide dismutase (EC 1.15.1.1) and catalase (EC 1.11.1.6) were measured in homogenate supernatant fractions of liver, heart, intestine, gizzard and skeletal muscle of deficient ducklings and of control birds given vitamin E or Se or both.

3. Glutathione peroxidase activities were dramatically lower in tissues of Se-deficient ducklings, and this was unaffected by vitamin E. No adaptive changes were seen in the activity of the other enzymes, even after 21 d when the deficiency was severe.

4. It appeared likely that the variability of the enzyme activities, other than glutathione peroxidase, in the different tissues studied might explain differences among the tissues in susceptibility to peroxidative damage.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 50 , Issue 2 , September 1983 , pp. 437 - 444
Copyright
Copyright © The Nutrition Society 1983

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