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Gastrointestinal implications in pigs of wheat and oat fractions

1. Digestibility and bulking properties of polysaccharides and other major constituents

Published online by Cambridge University Press:  09 March 2007

K. E. Bach Knudsen  and
Inge Hansen
K. E. Bach Knudsen
Affiliation:
National Institute of Animal Science, Department of Animal Physiology and Biochemistry, Foulum P.O. Box 39, DK-8830 Tjele, Denmark
Inge Hansen
Affiliation:
National Institute of Animal Science, Department of Animal Physiology and Biochemistry, Foulum P.O. Box 39, DK-8830 Tjele, Denmark
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Abstract

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The present work was undertaken to study the gastrointestinal effects of wheat and oat dietary fibre (DF) using 40–50 kg pigs cannulated in the terminal ileum. The variables studied were: chemical characteristics of the DF, ileal and faecal digestibility of nutrients and bulking properties of polysaccharides and other major constituents. The wheat products studied included refined wheat flour and wheat fractions rich in the following botanical components: aleurone, pericarp/testa and bran. The oat products used were rolled oats and oat bran. The products varied considerably in DF content (g/kg dry matter) and composition; non-starch polysaccharides (NSP) and Klason lignin content ranged from 34 and 1 g/kg respectively in wheat flour, to 465 and 92 g/kg in pericarp/testa. The main NSPs in the wheat were arabinoxylans (AX) (64–69%) and cellulose (15–31%) and in oats mixed linked β(1 → 3; 1 → 4)-D-glucans (β-glucans; 46–63%) and AX (28–32%). The lowest content of soluble NSP was found in the lignified wheat fractions (bran and pericarp/testa) and the highest in oat bran. Eight diets were produced using the wheat and oat products and studied in two series of experiments using wheat flour as the DF-depleted control. The diets in Expt 1 were based on wheat flour and three iso-DF enriched diets prepared by adding DF from the fractions rich in wheat aleurone, pericarp/testa or bran. In Expt 2, oat bran was added to wheat flour to achieve the same DF intake level as in Expt 1. This series also included diets based on rolled oats and rolled oats plus oat bran. Starch was almost completely digested in the small intestine (0.97–1.00). However, there was a tendency to a slightly lower digestibility of oat starch compared with wheat starch. The recovery of wheat NSP in ileal digesta was 82–104 % compared with 64–66% for oats. The low recovery of NSP in oat diets was primarily due to the low recovery of β-glucans (25–36%). In the large intestine NSP and starch residues were extensively degraded. For the DF-depleted control diets or diets based on oats, 8–17% NSP survived breakdown while in the diets enriched with aleurone, pericarp/testa or bran fractions, NSP recovery was 33, 50 and 38 % respectively. Fermentative breakdown of carbohydrates in the large intestine was estimated to contribute between 10 and 24 % of the energy for maintenance. Energy derived from the inflow of organic acids from the ileum contributed an additional 1–4% of maintenance energy. In wheat endosperm, AX were broken down to a greater extent than cellulose, while the breakdown of AX in pericarp/testa was similar to that of cellulose. This difference in NSP breakdown can be explained by structural differences in the two types of cell walls. The breakdown of oat AX was lower than that of wheat flour. Wheat DF increased faecal bulk primarily by virtue of its physical presence and its water-holding capacity, while the oat DF stimulated faecal output through an increase in microbial biomass (Bach Knudsen et al. 1991). The result was a higher excretion of protein and fat. The higher fat excretion with the oat diets was probably due to a higher bile acid excretion caused by the more extensive fermentation of carbohydrates and the lower lumen pH.

Keywords

PolysaccharidesDietary fibreDigestibilityPig
Type
Diet and its Effects on Gastrointestinal Function
Information
British Journal of Nutrition , Volume 65 , Issue 2 , March 1991 , pp. 217 - 232
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Anderson, J. W. & Chen, W. L. (1979). Plant fiber. Carbohydrate and lipid metabolism. American Journal of Clinical Nutrition 32, 346–363.CrossRefGoogle ScholarPubMed
Aspinall, G. O. & Carpenter, R. C. (1984). Structural investigations on the non-starchy polysaccharides of oat bran. Carbohydrate Polymers 4, 271–282.CrossRefGoogle Scholar
Association of Official Analytical Chemists (1975). Official Methods of Analysis, 11th ed. Washington DC: Association of Official Analytical Chemists.Google Scholar
Bach Knudsen, K. E., Åman, P. & Eggum, B. O. (1987). Nutritive value of Danish-grown barley varieties. I. Carbohydrates and other major constituents. Journal of Cereal Science 6, 173–186.CrossRefGoogle Scholar
Bach Knudsen, K. E., Borg Jensen, B., Andersen, J. O. & Hansen, I. (1991). Gastrointestinal implications in pigs of wheat and oat fractions. 2. Microbial activity in the gastrointestinal tract. British Journal of Nutrition 65, 233–248.CrossRefGoogle ScholarPubMed
Bach Knudsen, K. E. & Eggum, B. O. (1984). The nutritive value of botanically defined mill fractions of barley. 3. The protein and energy value of pericarp, testa, germ, aleuron, and endosperm rich decortication fractions of the variety Bomi. Zeitschrift für Tierphysiologie, Tierernährung und Futtermittelkunde 51, 130–148.CrossRefGoogle Scholar
Bach Knudsen, K. E., Hansen, I., Borg Jensen, B. & Østergård, K. (1990). Physiological implications of wheat and oat dietary fiber. In Dietary Fiber – New Developments: Physiological Effects and Physiochemical Properties, pp. 135–150 [Furda, I. and Brine, C. J., editors]. New York: Plenum Press.CrossRefGoogle Scholar
Bach Knudsen, K. E., Wolstrup, J. & Eggum, B. O. (1984). The nutritive value of botanically defined mill fractions of barley. 4. The influence of hind-gut microflora in rats on digestibility of protein and energy of pericarp, testa, germ, aleuron and endosperm rich decortication fractions of the variety Bomi. Zeitschrift für Tierphysiologie, Tierernährung und Futtermittelkunde 52, 182–193.CrossRefGoogle Scholar
Bacic, A. & Stone, B. A. (1981a). Isolation and ultrastructure of aleurone cell walls from wheat and barley. Australian Journal of Plant Physiology 8, 453–474.Google Scholar
Bacic, A. & Stone, B. A. (1981b). Chemistry and organization of aleurone cell wall components from wheat and barley. Australian Journal of Plant Physiology 8, 475–495.Google Scholar
Chen, W. J. L. & Anderson, J. W. (1986). Hypocholesterolemic effects of soluble fibers. In Dietary Fiber. Basic and Clinical Aspects, pp. 275–286 [Vahouny, G. V. and Kritchevsky, D., editors]. New York: Plenum Press.CrossRefGoogle Scholar
Cheng, B.-Q., Trimble, R. P., Illman, R. J., Stone, B. A. & Topping, D. L. (1987). Comparative effects of dietary wheat bran and its morphological components (aleurone and pericarp-seed coat) on volatile fatty acid concentrations in the rat. British Journal of Nutrition 57, 69–76.CrossRefGoogle ScholarPubMed
Cummings, J. H., Southgate, D. A. T., Branch, W. J., Houston, H., Jenkins, D. J. A. & James, W. P. T. (1978). Colonic response to dietary fibre from carrot, cabbage, apple, bran and guar gum. Lancet i, 5–8.CrossRefGoogle Scholar
Donangelo, C. M. & Eggum, B. O. (1985). Comparative effects of wheat bran and barley husk on nutrient utilization in rats. 1. Protein and energy. British Journal of Nutrition 54, 741–751.CrossRefGoogle ScholarPubMed
Eggum, B. O., Thorbek, G., Beams, R. M., Chwalibog, A. & Henckel, S. (1982). Influence of diet and microbial activity in the digestive tract on digestibility, and nitrogen and energy metabolism in rats and pigs. British Journal of Nutrition 48, 161–175.CrossRefGoogle ScholarPubMed
Englyst, H. N. & Cummings, J. H. (1985). Digestion of the polysaccharides of some cereal foods in the human small intestine. American Journal of Clinical Nutrition 42, 778–787.CrossRefGoogle ScholarPubMed
Englyst, H. N., Wiggins, H. S. & Cummings, J. H. (1982). Determination of non-starch polysaccharides in plant foods by gas–liquid chromatography of constituent sugars as alditol acetates. Analyst 107, 307–318.CrossRefGoogle ScholarPubMed
Gawehn, K. (1984 d).– Lactate. In Methods of Enzymatic Analysis, vol. 6, pp. 588–592 [Bergmeyer, J. and Grasl, M., editors]. Weinheim: Verlag Chemie.Google Scholar
Graham, H., Hesselmann, K. & Åman, P. (1986a). The influence of wheat bran and sugar-beet pulp on the digestibility of dietary components in a cereal-based pig diet. Journal of Nutrition 116, 242–251.CrossRefGoogle Scholar
Graham, H., Hesselman, K., Jonsson, E. & Åman, P. (1986b). Influence of β-glucanase supplementation on digestion of a barley-based diet in the pig gastrointestinal tract. Nutrition Reports International 34, 1089–1096.Google Scholar
Holt, S., Heading, R. C., Carter, D. C., Prescott, L. F. & Tothill, P. (1979). Effects of gel fibre on gastric emptying and absorption of glucose and paracetamol. Lancet i, 636–639.CrossRefGoogle Scholar
Imoto, S. & Namioka, S. (1978). VFA production in the pig large intestine. Journal of Animal Science 47, 467–478.CrossRefGoogle ScholarPubMed
Jenkins, D. J. A., Wolever, T. M. S., Leeds, A. R., Gassull, M. A., Haisman, P., Dilawari, J., Goff, D. V., Metz, G. L. & Alberti, K. G. M. M. (1978). Dietary fibres, fibre analogues and glucose tolerance: importance of viscosity. British Medical Journal 1, 1392–1394.CrossRefGoogle ScholarPubMed
Jørgensen, K. G. & Aastrup, S. (1987). Determination of β-glucan in barley, malt, wort and beer. In Modern Methods of Plant Analysis, vol. 7, pp. 88–108 [Linskens, H. F. and Jackson, J. F., editors]. Berlin: Springer-Verlag.Google Scholar
Judd, P. A. & Truswell, S. A. (1981). The effect of rolled oats on blood lipids and fecal steroid excretion in man. American Journal of Clinical Nutrition 34, 2061–2067.CrossRefGoogle ScholarPubMed
Just, A. (1975). Feed evaluation in pigs. World Review of Animal Production 11, 18–30.Google Scholar
Keys, J. E. & DeBarthe, J. V. (1974). Site and extent of carbohydrate, dry matter, energy and protein digestion and the rate of passage of grain diets in swine. Journal of Animal Science 39, 57–62.CrossRefGoogle ScholarPubMed
Kirby, R. W., Anderson, J. W., Sieling, B., Rees, E. D., Chen, W. J. L., Miller, R. E. & Kay, R. M. (1981). Oat-bran intake selectively lowers serum low density lipoprotein cholesterol concentrations of hypercholesterolemic men. American Journal of Clinical Nutrition 34, 824–829.CrossRefGoogle ScholarPubMed
Larsson, K. & Bengtsson, S. (1983). Bestämning av lättilgängeliga kolhydrater i växtmaterial (Determination of readily available carbohydrates in plant material). Methods Report no. 22. Uppsala: National Laboratory of Agricultural Chemistry.Google Scholar
Mares, D. J. & Stone, B. A. (1973). Studies on wheat endosperm. I. Chemical composition and ultrastructure of the cell walls. Australian Journal of Biological Science 26, 793–812.Google Scholar
Markwalder, H. U. & Neulkom, H. (1976). Diferulic acid as a possible crosslink to hemicellulose from wheat germ. Phytochemistry 15, 836–937.CrossRefGoogle Scholar
Mason, V. C. (1980). Role of the large intestine in the processes of digestion and absorption in the pig. In Current Concept of Digestion and Absorption in Pigs, Technical Bulletin no. 3, pp. 112–129 [Low, A. G. and Patridge, I. G., editors]. Reading: National Institute for Research in Dairying, and Ayr, Scotland: Hannah Research Institute.Google Scholar
Mason, V. C. & Just, A. (1976). Bacterial activity in the hind-gut of pigs. 1. Its influence on the apparent digestibility of dietary energy and fat. Zeitschrift für Tierphysiologie, Tierernährung und Futtermittelkunde 36, 301–310.CrossRefGoogle ScholarPubMed
Millard, P. & Chesson, A. (1984). Modification to swede (Brassica napus L.) anterior to the terminal ileum of pigs: some implications for the analysis of dietary fibre. British Journal of Nutrition 52, 583–594.CrossRefGoogle Scholar
Neukom, H., Providoli, L., Gremli, H. & Hui, P. A. (1964). Recent investigation on wheat flour pentosans. Cereal Chemistry 44, 238–244.Google Scholar
Noll, F. (1984 d). (+)-Lactate. In Methods of Enzymatic Analysis, vol. 6, pp. 582–588 [Bergmeyer, J. and Grasl, M., editors]. Weinheim: Verlag Chemie.Google Scholar
Nyman, M. & Asp, N. G. (1982). Fermentation of dietary fibre components in the rat intestinal tract. British Journal of Nutrition 47, 357–366.CrossRefGoogle ScholarPubMed
Ring, S. R. & Selvendran, R. R. (1980). Isolation and analysis of cell wall material from beeswing wheat bran (Triticum aestivum). Phytochemistry 19, 1723–1730.CrossRefGoogle Scholar
Sandberg, A.-S., Ahderinne, R., Andersson, H., Hallgren, B. & Hulteén, L. (1983). The effect of citrus pectin on the absorption of nutrients in the small intestine. Human Nutrition: Clinical Nutrition 37C, 171–183.Google Scholar
Sandberg, A.-S., Anderson, H., Hallgren, B., Hasselblad, K., Isaksson, B. & Hultén, L. (1981). Experimental model for in vivo determination of dietary fibre and its effect on the absorption of nutrients in the small intestine. British Journal of Nutrition 45, 283–294.CrossRefGoogle ScholarPubMed
Schürch, A., Lloyd, L. E. & Crapton, E. W. (1950). The use of chromic oxide as an index for determining the digestibility of a diet. Journal of Nutrition 50, 629–636.CrossRefGoogle Scholar
Selvendran, R. R. (1983). The chemistry of plant cell walls. In Dietary Fibre, pp. 95–147 [Birch, G. G. and Parker, K. J., editors]. London: Applied Science Publishers.Google Scholar
Selvendran, R. R. (1984). The plant cell wall as a source of dietary fibre: chemistry and structure. American Journal of Clinical Nutrition 39, 320–337.Google ScholarPubMed
Snedecor, G. W. & Cochran, W. G. (1973). Statistical Method. Ames: Iowa State University Press.Google Scholar
Southgate, D. A. T., Branch, W. J., Hill, M. J., Drasar, B. S., Walters, R. L., Davies, P. S. & Baird, I. M. (1976). Metabolic responses to dietary supplements of bran. Metabolism 25, 1129–1135.CrossRefGoogle ScholarPubMed
Spiller, G. A., Story, J. A., Wong, L. G., Nunes, J. D., Alton, M., Petro, M. S., Furumoto, E. J., Whittam, J. H. & Scala, J. (1986). Effect of increasing levels of hard wheat fiber on fecal weight, minerals and steroids and gastrointestinal transit time in healthy young women. Journal of Nutrition 116, 778–785.CrossRefGoogle ScholarPubMed
Stephen, A. M. & Cummings, J. H. (1980). The microbial contribution to human faecal mass. Journal of Medical Microbiology 13, 45–56.CrossRefGoogle ScholarPubMed
Stoldt, W. (1957). Verslag zur vereinheitlichung der fettbestimmung in lebensmitteln. Fetten, Seifen, Anstrichmittel 54, 206–207.CrossRefGoogle Scholar
Theander, O. & Åman, P. (1979). Studies on dietary fibre. 1. Analysis and chemical characterization of water-soluble and water-insoluble dietary fibres. Swedish Journal of Agricultural Research 9, 97–106.Google Scholar
Theander, O. & Westerlund, E. A. (1986). Studies on dietary fiber. 3. Improved procedures for analysis of dietary fiber. Journal of Agricultural and Food Chemistry 34, 330–336.CrossRefGoogle Scholar
Wisker, E., Feldheim, W., Pomeranz, Y. & Meuser, F. (1985). Dietary fiber in cereals. In Advances in Cereal Science and Technology, vol. 7, pp. 169–238 [Pomeranz, Y., editor]. St Paul: American Association of Cereal Chemists.Google Scholar
Wood, P. J. (1986). Oat β-glucan: structure, location, and properties. In Oats: Chemistry and Technology, pp. 121–152 [Webster, F. H., editor]. St Paul: American Association of Cereal Chemists.Google Scholar
Wyman, J. B., Heaton, K. W., Manning, A. P. & Wicks, A. C. B. (1976). The effect on intestinal transit and the feces of raw and cooked bran in different doses. American Journal of Clinical Nutrition 29, 1474–1479.CrossRefGoogle ScholarPubMed