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Exposure to purified lignan from flaxseed (Linum usitatissimum) alters bone development in female rats

Published online by Cambridge University Press:  09 March 2007

Wendy E. Ward ,
Yvonne V. Yuan ,
Angela M Cheung  and
Lilian U. Thompson
Wendy E. Ward
Affiliation:
Department of Nutritional Sciences, University of Toronto, Toronto, Ontario M5S 3E2, Canada
Yvonne V. Yuan
Affiliation:
School of Nutrition, Ryerson Polytechnic University, Toronto, Ontario M5B 2K3, Canada
Angela M Cheung
Affiliation:
Osteoporosis Program, Department of Medicine, University Health Network & Mount Sinai Hospital, Toronto, Ontario
Lilian U. Thompson*
Affiliation:
Department of Nutritional Sciences, University of Toronto, Toronto, Ontario M5S 3E2, Canada
*
*Corresponding author: Dr Lilian U. Thompson, fax +1 416 978 5882, email [email protected]
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Abstract

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Due to the potential oestrogenic effects of secoisolariciresinol diglycoside (SDG), the mammalian lignan precursor in flaxseed (Linum usitatissimum), we hypothesized that exposure to purified SDG during early life would have a positive effect on developing bone. This present study determined whether exposure to SDG purified from flaxseed during suckling via mother's milk or continuously to adolescence (postnatal day (PND) 50) or adulthood (PND 132) increased bone mineral content (BMC) or bone strength in female rat offspring. Offspring were exposed to basal diet (BD) or one of two doses of SDG (50S, 100S) equivalent to that in a 50 or 100 g flaxseed/kg diet during lactation only or through to PND 50 or 132. At PND 50 and 132, femurs were analysed for BMC by dual energy X-ray absorptiometry and biomechanical strength by a 3-point bending test. Compared with BD group, rats exposed to continuous 50S or 100S diet had stronger femurs at PND 50 without changes in BMC. At PND 132 there were no differences in femur strength despite the fact that continuous exposure to BD resulted in a higher (P<0·05) BMC than rats exposed to 100S during lactation only or to 50S or 100S during lactation through to adulthood. In conclusion, female rat bone is more sensitive to the oestrogen-like action of lignans during early life when endogenous levels of sex hormones are low, but by adulthood the improved bone strength does not persist. Importantly, exposure to purified lignan does not have negative effects on bone strength.

Keywords

LignanBiomechanicsBone mineralDeveloping rats
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 4 , October 2001 , pp. 499 - 505
Copyright
Copyright © The Nutrition Society 2001

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