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Exogenous and endogenous nitrogen flow rates and level of protein hydrolysis in the human jejunum after [15N]milk and [15N]yoghurt ingestion

Published online by Cambridge University Press:  09 March 2007

Clarie Gaudichon ,
Sylvain Mahé ,
Nils Roos ,
Robert Benamouzig ,
Catherine Luengo ,
Jean-François Huneau ,
Hinrich Sick ,
Christine Bouley ,
Jaques Rautureau  and
Daniel Tome
Clarie Gaudichon
Affiliation:
Unité INRA de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Sylvain Mahé
Affiliation:
Unité INRA de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Nils Roos
Affiliation:
Institut für Physiologie und Biochemie der Ernährung, Bundesanstalt für Milchforschung, PO Box 6069, D-2300 Kiel 14, Germany
Robert Benamouzig
Affiliation:
Service de Gastroentérologie, Hôpital Avicenne, 125 route de Stalingrad, 93009 Bobigny Cédex, France
Catherine Luengo
Affiliation:
Unité INRA de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Jean-François Huneau
Affiliation:
Unité INRA de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Hinrich Sick
Affiliation:
Institut für Physiologie und Biochemie der Ernährung, Bundesanstalt für Milchforschung, PO Box 6069, D-2300 Kiel 14, Germany
Christine Bouley
Affiliation:
Groupe Danone, Branche Produits Frais, 15 avenue Galilée, 92350 Le-Plessis-Robinson, France
Jaques Rautureau
Affiliation:
Service de Gastroentérologie, Hôpital Avicenne, 125 route de Stalingrad, 93009 Bobigny Cédex, France
Daniel Tome
Affiliation:
Unité INRA de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
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Abstract

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Milk and yoghurt proteins were 15N-labelled in order to measure the flow rate of exogenous N during digestion in the human intestine. After fasting overnight, sixteen healthy volunteers, each with a naso-jejunal tube, ingested either [15N]milk (n 7) or [15N]yoghurt (n 9). Jejunal samples were collected every 20 min for 4 h. A significant stimulation of endogenous N secretion was observed during the 20–60 min period after yoghurt ingestion and the 20–40 min period after milk ingestion. The endogenous N flows over a 4 h period did not differ between the groups (44·3 (SEM 6·5) mmol for milk and 63·5 (SEM 5·9) mmol for yoghurt). The flow rates of exogenous N indicated a delayed gastric emptying of the yoghurt N compared with N from milk. The jejunal non-protein N (NPN) flow rate increased significantly after milk and yoghurt ingestion due to an increase in the exogenous NPN flow rate. The NPN fraction of exogenous N ranged between 40 and 80%. The net gastro-jejunal absorption of exogenous N did not differ significantly between milk (56·7 (SEM 8·5)%) and yoghurt (50·9 (SEM 7)%). The high level of exogenous N hydrolysis is in accordance with the good digestibility of milk products. Fermentation modifies only the gastric emptying rate of N and does not affect the level of diet hydrolysis, the endogenous N stimulation or the digestibility rate.

Keywords

Milk proteins15NDigestionHumanJejunum
Type
Digestion of protein from milk and yoghurt
Information
British Journal of Nutrition , Volume 74 , Issue 2 , August 1995 , pp. 251 - 260
Copyright
Copyright © The Nutrition Society 1995

References

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