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Evaluation of inert markers for the determination of ileal and faecal apparent digestibility values in the pig

Published online by Cambridge University Press:  09 March 2007

S. Jagger ,
J. Wiseman ,
D. J. A. Cole  and
J. Craigon
S. Jagger
Affiliation:
Department of Agriculture and Horticulture, University of Nottingham, Faculty of Agricultural and Food Sciences, Sutton Bonington, Loughborough, Leics LE12 5RD
J. Wiseman
Affiliation:
Department of Agriculture and Horticulture, University of Nottingham, Faculty of Agricultural and Food Sciences, Sutton Bonington, Loughborough, Leics LE12 5RD
D. J. A. Cole
Affiliation:
Department of Agriculture and Horticulture, University of Nottingham, Faculty of Agricultural and Food Sciences, Sutton Bonington, Loughborough, Leics LE12 5RD
J. Craigon
Affiliation:
Department of Physiology and Environmental Studies, University of Nottingham, Faculty of Agricultural and Food Sciences, Sutton Bonington, Loughborough, Leics LE12 5RD
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Abstract

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A series of experiments was designed to evaluate inert markers employed in studies of ileal and faecal apparent digestibilities of nitrogen and amino acids in pigs fitted with simple ‘T’ piece cannulas. Trial 1 assessed the palatability of diets containing (a) 5 g chromic oxide/kg, (b) 1 and (c) 5 g titanium dioxide/kg at two levels of feeding. The results indicated that there were slight problems in terms of period of feed consumption associated with diets (a) and (c), but that these effects were transitory and disappeared following acclimatization, although higher levels of feeding may compound the problem. Trial 2 estimated N balance, faecal apparent digestibility of amino acids as determined by both total collection and ratio of markers, ileal apparent digestibility of amino acids and recovery of markers using diets containing (a) 1 and (b) 5 g Cr2O3/kg, (c) 1 and (d) 5 g TiO2/kg. An additional assessment of acid-insoluble lignin present within the diet was also undertaken. Cr2O3 was associated with the lowest recovery, whilst calculations based on TiO2 gave lower standard errors. Trial 3 examined diurnal variation in digesta sampling and its effect on faecal apparent digestibility of N and amino acids. There were no evident effects. In general, it was concluded that the most appropriate marker to use in studies of this nature was TiO2 at a rate of 1 g/kg.

Keywords

Inert markersIleal digestibilityFaecal digestibilityPig
Type
Measurement of Intestinal Functions
Information
British Journal of Nutrition , Volume 68 , Issue 3 , November 1992 , pp. 729 - 739
Copyright
Copyright © The Nutrition Society 1992

References

Agricultural Research Council (1981). The Nutrient Requirements of Pigs. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Barnicote, C. R. (1945). Estimation of apparent digestibility coefficients by means of an inert reference substance. New Zealand Journal of Science and Technology A 27, 202–212.Google Scholar
Bech-Anderson, S. (1979). Single-column analysis of amino acids in hydrolysates containing chromic oxide. Journal of Chromatography 179, 227–228.CrossRefGoogle Scholar
Buttery, P. J. & Soar, J. B. (1975). A spectrofluorimetric assay of the tryptophan content of feedstuffs. Journal of the Science of Food and Agriculture 26, 1273–1277.CrossRefGoogle Scholar
Corring, T. (1980). Endogenous secretions in the pig. In Current Concepts of Digestion and Absorption in Pigs, pp. 136–150 [Low, A. G. and Partridge, I. G., editors]. Reading: National Institute for Research in Dairying.Google Scholar
Fenton, T. W. & Fenton, M. (1979). An improved procedure for the determination of chromic oxide in feed and feces. Canadian Journal of Animal Science 59, 631–634.CrossRefGoogle Scholar
Jagger, S. (1987). Ileal digestibility of pig diets. PhD Thesis, University of Nottingham.Google Scholar
Kane, E. A., Jacobsen, W. C. & Moore, L. A. (1950). A comparison of techniques used in digestibility studies with dairy cattle. Journal of Nutrition 41, 583–596.CrossRefGoogle ScholarPubMed
Kotb, A. R. & Luckey, T. D. (1972). Markers in nutrition. Nutrition Abstracts and Reviews 42, 813–845.Google ScholarPubMed
Leone, J. L. (1973). Collaborative study of the quantitative determination of titanium dioxide in cheese. Journal of the Association of Official Analytical Chemists 56, 535–537.Google ScholarPubMed
Lloyd, L. E., Rutherford, B. E. & Crampton, E. W. (1955). A comparison of titanic oxide and chromic oxide as index materials for determining apparent digestibility. Journal of Nutrition 56, 265–271.CrossRefGoogle ScholarPubMed
Low, A. G. (1982). Digestibility and availability of amino acids from feedstuffs for pigs: a review. Livestock Production Science 9, 511–520.CrossRefGoogle Scholar
Marsden, M. (1984). Amino acid metabolism in the rumen. PhD Thesis, University of Nottingham.Google Scholar
Maynard, L. A., Loosli, J. K., Hintz, H. F. & Warner, R. G. (1979). Animal Nutrition, 7th ed. London: McGraw-Hill Book Company.Google Scholar
Moore, J. H. (1957). Diurnal variations in the composition of faeces of pigs on diets containing chromium oxide. British Journal of Nutrition 11, 173–288.CrossRefGoogle ScholarPubMed
Moore, J. H. (1959). The use of indicators in digestibility studies. Agricultural Progress 34, 48–63.Google Scholar
Morgan, D. J. (1972). Energy values in pig nutrition. PhD Thesis, University of Nottingham.Google Scholar
Njaa, L. R. (1961). Determination of protein digestibility with titanium oxide as indicator substance. Acta Agriculturae Scandinavica 11, 227–241.CrossRefGoogle Scholar
Peddie, J., Dewar, W. A., Gilbert, A. B. & Waddington, D. (1982). The use of titanium dioxide for determining apparent digestibility in mature domestic fowls (Callus domesticus). Journal of Agricultural Science, Cambridge 99, 233–236.Google Scholar
Peers, D. G., Taylor, A. G. & Whittemore, C. T. (1977). The influence of feeding level and level of dietary inclusion on the digestibility of barley meal in the pig. Animal Feed Science and Technology 2, 41–47.CrossRefGoogle Scholar
Rowell, J. G. & Walters, D. E. (1976). Analysis of data with repeated observations on each experimental unit. Journal of Agricultural Science, Cambridge 87, 423–432.CrossRefGoogle Scholar
Steele, W. & Clapperton, J. L. (1982). The use of chromic oxide as a food marker - a warning. Journal of the Science of Food and Agriculture 33, 325–328.CrossRefGoogle Scholar
Tanksley, T. D. Jr, Knabe, D. A., Purser, K., Zebrowska, T. & Corley, J. R. (1981). Apparent digestibility of amino acids and nitrogen in three cottonseed meals and one soybean meal. Journal of Animal Science 52, 769–777.CrossRefGoogle Scholar
Taylor, J. (1973). The analysis of designed experiments with censored observations. Biometrics 29, 35–43.CrossRefGoogle Scholar
Van Soest, P. J. (1963). Use of detergents in the analysis of fibrous feeds. II. A rapid method for the determination of fibre and lignin. Journal of the Association of Official Analytical Chemists 46, 829–835.Google Scholar
Wishart, J. (1938). Growth rate determinations in nutrition studies with the bacon pig and their analysis. Biometrika 30, 16–28.CrossRefGoogle Scholar