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Ethanol with a mixed meal increases postprandial triacylglycerol but decreases postprandial non-esterified fatty acid concentrations

Published online by Cambridge University Press:  09 March 2007

Barbara A. Fielding ,
Guy Reid Michelle Grady ,
Sandy M. Humphreys ,
Kevin Evans  and
Keith N. Frayn
Barbara A. Fielding*
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford OX2 6HE, UK
Guy Reid Michelle Grady
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford OX2 6HE, UK
Sandy M. Humphreys
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford OX2 6HE, UK
Kevin Evans
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford OX2 6HE, UK
Keith N. Frayn
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford OX2 6HE, UK
*
*Corresponding author: Dr Barbara Fielding, fax +44 (0) 1865 224872, email [email protected]
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Abstract

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Plasma triacylglycerol concentrations increase after the acute ingestion of alcohol (specifically ethanol). However, the effect of ethanol when consumed with a mixed meal has not been well studied. The objective of the present study was to determine the perturbations of lipid metabolism that occur after ingestion of ethanol in combination with a mixed meal of specific fatty acid composition. Blood samples were taken from seven healthy male subjects before and after a mixed meal, with and without ethanol. The specific fatty acid composition of the test meal allowed the fatty acids to be traced into the plasma non-esterified fatty acid pool during the postprandial period. Statistical analysis by repeated measures ANOVA showed significant effects of ethanol. For example, postprandial lipaemia was enhanced after the ethanol test meal compared with the control (P < 0·05), mainly due to increases in triacylglycerol-rich lipoproteins in the flotation range Sf 60–400 (VLDL1) (P < 0·05); those in the range Sf 20–60 (VLDL2) and also Sf > 400 (chylomicrons) were not significantly affected. The later postprandial increase in plasma non-esterified fatty acid concentrations was reduced after the ingestion of ethanol (P < 0·001), but the proportions of palmitoleic acid (a marker of fatty acid content of the test meal) and of linoleic acid (a marker of endogenous lipolysis) were not affected. The results suggest a primary effect of ethanol on the stimulation of secretion of large VLDL particles, which then compete for clearance with chylomicrons by lipoprotein lipase. The results do not support an effect of ethanol on the release of non-esterified fatty acid into the plasma. The suppression of plasma non-esterified fatty acid concentrations during the postprandial period may contribute towards the beneficial effects of moderate ethanol consumption.

Keywords

Postprandial lipid metabolismNon-esterified fatty acidsEthanol
Type
Research Article
Information
British Journal of Nutrition , Volume 83 , Issue 6 , June 2000 , pp. 597 - 604
Copyright
Copyright © The Nutrition Society 2000

References

Abraha, AHumphreys, SMClark, ML, Matthews, DR and Frayn, KN (1998) Acute effect of fructose on postprandial lipaemia in diabetic and non-diabetic subjects British Journal of Nutrition 80, 169–175.CrossRefGoogle ScholarPubMed
Abrams, M and Cooper, C (1976) Mechanism of increased hepatic uptake of unesterified fatty acid from serum of ethanol-treated rats Biochemical Journal 156, 47–54.CrossRefGoogle ScholarPubMed
Abrams, M and Cooper, C (1976) Quantitative analysis of metabolism of hepatic triglyceride in ethanol-treated rats Biochemical Journal 156, 33–46.CrossRefGoogle ScholarPubMed
Baraona, E and Lieber, C (1979) Effects of ethanol on lipid metabolism Journal of Lipid Research 20, 289–315.CrossRefGoogle ScholarPubMed
Betteridge, D & Morrel, J (1998) Clinicians' Guide to Lipids and Coronary Heart Disease. London: Chapman and Hall.Google Scholar
Christiansen, CThomsen, CRasmussen, O, Hansen, C and Hermansen, K (1996) The acute impact of ethanol on glucose, insulin, triacylglycerol, and free fatty acid responses and insulin sensitivity in type 2 diabetes British Journal of Nutrition 76, 669–675.CrossRefGoogle ScholarPubMed
Cohen, JC and Schall, R (1988) Reassessing the effects of simple carbohydrates on the serum triglyceride responses to fat meals American Journal of Clinical Nutrition 48, 1031–1034.CrossRefGoogle ScholarPubMed
Coppack, SWEvans, RDFisher, RM, Frayn, KNGibbons, GF, Humphreys, SMKirk, ML, Potts, JL and Hockaday, TDR (1992) Adipose tissue metabolism in obesity: lipase action in vivo before and after a mixed meal Metabolism 41, 264–272.CrossRefGoogle ScholarPubMed
Crouse, JRGerson, CD DeCarli LM and Lieber, CS (1968) Role of acetate in the reduction of plasma free fatty acids produced by ethanol in man Journal of Lipid Research 9, 509–512.CrossRefGoogle ScholarPubMed
Fielding, BACallow, J Owen M, Samra, JSMatthews, DR and Frayn, KN (1996) Postprandial lipaemia: the origin of an early peak studied by specific dietary fatty acid intake during sequential meals American Journal of Clinical Nutrition 63, 36–41.CrossRefGoogle ScholarPubMed
Franceschini, GMoreno, YApebe, P, Calabresi, LGatti, E, Noe, Dde Fabiani, EZoppi, F and Sirtori, C (1988) Alterations in high-density lipoprotein subfractions during postprandial lipidaemia induced by fat with and without ethanol Clinical Science 75, 135–142.CrossRefGoogle ScholarPubMed
Frayn, KNCoppack, SWWalsh, PE, Butterworth, HCHumphreys, SM and Pedrosa, HC (1990) Metabolic responses of forearm and adipose tissues to acute ethanol ingestion Metabolism 39, 958–966.CrossRefGoogle ScholarPubMed
Frayn, KNSummers, LKM and Fielding, BA (1997) Regulation of the plasma non-esterified fatty acid concentration in the postprandial state Proceedings of the Nutrition Society 56, 713–721.CrossRefGoogle ScholarPubMed
Frayn, KNWilliams, CM and Arner, P (1996) Are increased plasma non-esterified fatty acid concentrations a risk marker for coronary heart disease and other chronic diseases? Clinical Science 90, 243–253.CrossRefGoogle ScholarPubMed
Goldberg, CTall, A and Krumholz, S (1984) Acute inhibition of hepatic lipase and increase in plasma lipoproteins after alcohol intake Journal of Lipid Research 25, 714–720.CrossRefGoogle ScholarPubMed
Holland, BA, Welch, AA, Unwin, ID, Buss, DH, Paul, AA & Southgate, DAT (1991) McCance and Widdowson's The Composition of Foods, 5th ed. Cambridge: Royal Society of Chemistry and Ministry of Agriculture, Fisheries and Food.Google Scholar
Humphreys, SM and Frayn, KN (1988) Micro-method for preparing perchloric extracts of blood Clinical Chemistry 34, 1657.CrossRefGoogle ScholarPubMed
Jeppesen, JChen, Y-DI, Zhou, M-Y, Schaaf, PCoulston, A and Reaven, GM (1995) Postprandial triglyceride and retinyl ester responses to oral fat: effects of fructose American Journal of Clinical Nutrition 61, 787–791.CrossRefGoogle ScholarPubMed
Karpe, FHumphreys, SMSamra, JS, Summers, LKM and Frayn, KN (1997) Clearance of lipoprotein remnant particles in adipose tissue and muscle in humans Journal of Lipid Research 38, 2335–2343.CrossRefGoogle ScholarPubMed
Konstantinov, V (1998) Editorial comment Current Opinion in Lipidology 9, 363–365.CrossRefGoogle Scholar
Lundquist, ETygstrup, KWinkler, K, Mellengaard, K and Munch-Petersen, S (1962) Ethanol metabolism and production of free acetate in the human liver Journal of Clinical and Laboratory Investigation 41, 955–961.CrossRefGoogle ScholarPubMed
Malmstrom, R, Packard, CJCaslake, MBedford, D, Stewart, PYki-Jarvinen, H, Shepherd, J and Taskinen, MR (1998) Effects of insulin and acipimox on VLDL1 and VLDL2 apolipoprotein B production in normal subjects Diabetes 47, 779–787.CrossRefGoogle ScholarPubMed
Mendeloff, A (1954) Effect of intravenous infusions of ethanol upon estimated hepatic blood flow in man Journal of Clinical Investigation 33, 1298–1302.CrossRefGoogle ScholarPubMed
Nilsson-Ehle, P (1978) Effects of ethanol intake on lipoprotein lipase activity in adipose tissue of fasting subjects Lipids 13, 433–437.CrossRefGoogle ScholarPubMed
Pownall, H (1994) Dietary ethanol is associated with reduced lipolysis of intestinally derived lipoproteins Journal of Lipid Research 35, 2105–2113.CrossRefGoogle ScholarPubMed
Reid, GGrady, MFielding, B, Humphreys, SEvans, K and Frayn, K (1999) Potentiation of postprandial lipaemia by ethanol Proceedings of the Nutrition Society 58, 122A.Google Scholar
Rimm, EKlatsky, AGrobbee, D and Stampfer, M (1996) Review of moderate alcohol consumption and reduced risk of coronary heart disease: is the effect due to beer, wine, or spirits? British Medical Journal 312, 731–736.CrossRefGoogle ScholarPubMed
Siler, SNeese, RParks, E and Hellerstein, M (1998) VLDL-triglyceride production after alcohol ingestion, studied using [2–13C1]glycerol Journal of Lipid Research 39, 2319–2328.CrossRefGoogle ScholarPubMed
Talbott, G and Keating, B (1962) Effects of preprandial whiskey on postalimentary lipemia Geriatrics 17, 802–808.Google ScholarPubMed
Truswell, AS (1994) Food carbohydrates and plasma lipids - an update American Journal of Clinical Nutrition 59, 710S–718S.CrossRefGoogle ScholarPubMed
Van Tol, AGroener, J, Scheek, LVan Gent, TVeenstra, J, Van de Pol, HHendriks, H and Schaafsma, G (1995) Induction of net mass lipid transfer reactions in plasma by wine consumption with dinner European Journal of Clinical Investigation 25, 390–395.Google ScholarPubMed
Zilversmit, DB (1979) Atherogenesis: a postprandial phenomenon Circulation 60, 473–485.CrossRefGoogle ScholarPubMed