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Eicosapentaenoic acid and 3,10 dithia stearic acid inhibit the desaturation of trans-vaccenic acid into cis-9, trans-11-conjugated linoleic acid through different pathways in Caco-2 and T84 cells

Published online by Cambridge University Press:  08 March 2007

Renaville Bénédicte ,
Anne Mullen ,
Fiona Moloney ,
Yvan Larondelle ,
Yves-Jacques Schneider  and
Helen M. Roche
Renaville Bénédicte
Affiliation:
Laboratoire de Biochimie cellulaire, Institut des Sciences de la Vie, Université catholique de Louvain, Louvain-la-Neuve, Belgium Unité de Biochimie de la Nutrition, Institut des Sciences de la Vie, Université catholique de Louvain, Louvain-la-Neuve, Belgium Fisiologia Veterinaria e Nutrizione, Dept Science degli Alimenti, Università degli Studi di Udine, Italy
Anne Mullen
Affiliation:
Nutrigenomics Research Group, Department of Clinical Medicine, Trinity College Dublin, St James's Hospital, James's Street, Dublin 8, Republic of Ireland
Fiona Moloney
Affiliation:
Nutrigenomics Research Group, Department of Clinical Medicine, Trinity College Dublin, St James's Hospital, James's Street, Dublin 8, Republic of Ireland
Yvan Larondelle
Affiliation:
Laboratoire de Biochimie cellulaire, Institut des Sciences de la Vie, Université catholique de Louvain, Louvain-la-Neuve, Belgium
Yves-Jacques Schneider
Affiliation:
Unité de Biochimie de la Nutrition, Institut des Sciences de la Vie, Université catholique de Louvain, Louvain-la-Neuve, Belgium
Helen M. Roche*
Affiliation:
Nutrigenomics Research Group, Department of Clinical Medicine, Trinity College Dublin, St James's Hospital, James's Street, Dublin 8, Republic of Ireland
*
*Corresponding author: Dr Helen Roche, fax +353 1 4542043, email [email protected]
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Abstract

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Stearoyl-CoA desaturase (SCD) is a key enzyme that determines the composition and metabolic fate of ingested fatty acids, in particular the conversion of trans-vaccenic acid (TVA) to conjugated linoleic acid (CLA). The present study addressed the hypothesis that intestinal TVA absorption and biotransformation into CLA can be modulated by EPA and 3,10-dithia stearic acid (DSA) via altered SCD mRNA levels and desaturation indices (cis-9, trans-11-CLA:TVA and oleic acid:stearic acid ratios) in Caco-2 and T84 cells, two well-established in vitro models of the human intestinal epithelium. The study determined the effect of acute (3h with 0·3mm-EPA or 0·3mm-DSA) and acute-on-chronic (1 week with 0·03mm-EPA or -DSA, followed by respectively, 0·3mm-EPA or -DSA for 3h) treatments. In both cell lines, acute EPA treatment did not alter SCD desaturation indices, whereas the acute-on-chronic treatment affected these surrogate markers of SCD activity. This was associated with reduced sterol regulatory-element binding protein-1c and SCD mRNA levels. In contrast, acute and acute-on-chronic DSA treatments significantly reduced SCD desaturation indices without affecting SCD mRNA levels in Caco-2 cells. The present study on intestinal cells shows that the conversion rate of TVA to c9, t11-CLA is affected by other fatty acids present in the diet such as EPA, confirming previous observations in hepatic and mammary cell models.

Keywords

Stearoyl-CoA desaturaseTrans fatty acidsConjugated linoleic acid
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 4 , April 2006 , pp. 688 - 695
Copyright
Copyright © The Nutrition Society 2006

References

Archibeque, SL, Lunt, DK, Gilbert, CD, Turner, RK & Smith, SBFatty acid indices of stearoyl-CoA desaturase do not reflect actual stearoyl-CoA desaturase enzyme activities in adipose tissues of beef steers finished with corn-, flaxseed-, or sorghum-based diets. J Anim Sci (2005) 83, 1153–1166.CrossRefGoogle ScholarPubMed
Astorg, P, Arnault, N, Czernichow, S, Noisette, N, Galan, P & Hercberg, SDietary intakes and food sources of n-6 and n-3 PUFA in French adult men and women. Lipids (2004) 39, 527–535.CrossRefGoogle ScholarPubMed
Belury, MADietary conjugated linoleic acid in health: physiological effects and mechanisms of action. Annu Rev Nutr (2002) 22, 505–531.CrossRefGoogle ScholarPubMed
Black, IL, Roche, HM & Gibney, MJAcute and chronic effects of conjugated linoleic acid (CLA) isomers (trans-10, cis-12 CLA and cis-9, trans-11 CLA) on human colon carcinoma (Caco-2) cell line lipid metabolism. J Nutr (2002 a) 132, 2167–2173.CrossRefGoogle ScholarPubMed
Black, IL, Roche, HM, Tully, AM & Gibney, MJAcute-onchronic effects of fatty acids on intestinal triacylglycerol-rich lipoprotein metabolism. Br J Nutr (2002 b) 88, 661–669.CrossRefGoogle Scholar
Bligh, EG & Dyer, WJA rapid method of total lipid extraction and purification. Can J Biochem Physiol (1959) 37, 911–917.CrossRefGoogle ScholarPubMed
Corl, BA, Barbano, DM, Bauman, DE & Ip, C4 Cis-9, trans-11 CLA derived endogenously from trans-11-18:1 reduces cancer risk in rats. J Nutr (2003) 133, 2893–2900.CrossRefGoogle Scholar
Field, CJ & Schley, PDEvidence for potential mechanisms for the effect of conjugated linoleic acid on tumor metabolism and immune function: lessons from n-3 fatty acids. Am J Clin Nutr (2004) 79, 1190S–1198S.CrossRefGoogle ScholarPubMed
Field, FJ, Born, E, Murthy, S & Mathur, SNPolyunsaturated fatty acids decrease the expression of sterol regulatory element-binding protein-1 in CaCo-2 cells: effect on fatty acid synthesis and triacylglycerol transport. Biochem J (2002) 368, 855–864.CrossRefGoogle ScholarPubMed
Gruffat, D, De La Torre, A, Chardigny, JM, Durand, D, Loreau, O & Bauchart, DVaccenic acid metabolism in the liver of rat and bovine. Lipids (2005) 40, 295–301.CrossRefGoogle ScholarPubMed
Hovik, KE, Spydevold, OS & Bremer, JThia fatty acids as substrates and inhibitors of stearoyl-CoA desaturase. Biochim Biophys Acta (1997) 1349, 251–256.CrossRefGoogle ScholarPubMed
Kim, YC & Ntambi, JMRegulation of stearoyl-CoA desaturase genes: role in cellular metabolism and preadipocyte differentiation. Biochem Biophys Res Commun (1999) 266, 1–4.CrossRefGoogle ScholarPubMed
Lin, X, Loor, JJ & Herbein, JHTrans10, cis12-18:2 is a more potent inhibitor of de novo fatty acid synthesis and desaturation than cis9, trans11-18:2 in the mammary gland of lactating mice. J Nutr (2004) 134, 1362–1368.CrossRefGoogle Scholar
Lock, AL, Corl, BA, Barbano, DMBauman, DE & Ip, CThe anticarcinogenic effect of trans-11 18:1 is dependent on its conversion to cis-9, trans-11 CLA by delta 9-desaturase in rats. J Nutr (2004) 134, 2698–2704.CrossRefGoogle ScholarPubMed
Loor, JJ, Lin, X & Herbein, JHDietary trans-vaccenic acid (trans11-18:1) increases concentration of cis9, trans11-conjugated linoleic acid (rumenic acid) in tissues of lactating mice and suckling pups. Reprod Nutr Dev (2002) 42, 85–99.CrossRefGoogle ScholarPubMed
Loor, JJ, Lin, X & Herbein, JHEffects of dietary cis 9, trans 11-18:2, trans 10, cis 12-18:2, or vaccenic acid (trans 11-18:1) during lactation on body composition, tissue fatty acid profiles, and litter growth in mice. Br J Nutr (2003) 90, 1039–1048.CrossRefGoogle ScholarPubMed
McLeod, RS, LeBlanc, AM, Langille, MA, Mitchell, PL & Currie, DLConjugated linoleic acids, atherosclerosis, and hepatic very-low-density lipoprotein metabolism. Am J Clin Nutr (2004) 79, 1169S–1174S.CrossRefGoogle ScholarPubMed
Madsen, L, Froyland, L, Grav, HJ & Berge, RKUp-regulated delta 9-desaturase gene expression by hypolipidemic peroxisomeproliferating fatty acids results in increased oleic acid content in liver and VLDL: accumulation of a delta 9-desaturated metabolite of tetradecylthioacetic acid. J Lipid Res (1997) 38, 554–563.CrossRefGoogle ScholarPubMed
Nakamura, MT & Nara, TYGene regulation of mammalian desaturases. Biochem Soc Trans (2002) 30, 1076–1079.CrossRefGoogle ScholarPubMed
Ntambi, JMRegulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. J Lipid Res (1999) 40, 1549–1558.CrossRefGoogle ScholarPubMed
Ntambi, JM & Miyazaki, MRegulation of stearoyl-CoA desaturases and role in metabolism. Prog Lipid Res (2004) 43, 91–104.CrossRefGoogle Scholar
O'Shea, M, Bassaganya-Riera, J & Mohede, ICImmunomodulatory properties of conjugated linoleic acid. Am J Clin Nutr (2004) 79, 1199S–1206S.CrossRefGoogle ScholarPubMed
Park, Y, Albright, KJ, Cai, ZY & Pariza, MWComparison of methylation procedures for conjugated linoleic acid and artifact formation by commercial (trimethylsilyl) diazomethane. J Agric Food Chem (2001) 49, 1158–1164.CrossRefGoogle ScholarPubMed
Roche, HM, Noone, E, Sewter, C, McBennett, SSavage, DGibney, MJO'Rahilly, S & Vidal-Puig, AJIsomer-dependent metabolic effects of conjugated linoleic acid: insights from molecular markers sterol regulatory element-binding protein-1c and LXRalpha. Diabetes (2002) 51, 2037–2044.CrossRefGoogle ScholarPubMed
Sessler, AM, Kaur, N, Palta, JP & Ntambi, JMRegulation of stearoyl-CoA desaturase-1 mRNA stability by polyunsaturated fatty acids in 3T3-L1 adipocytes. J Biol Chem (1996) 271, 29854–29858.CrossRefGoogle ScholarPubMed
Sewter, C, Berger, D, Considine, RVet al.. Human obesity and type 2 diabetes are associated with alterations in SREBP1 isoform expression that are reproduced ex vivo by tumor necrosis factoralpha. Diabetes (2002) 51, 1035–1041.CrossRefGoogle Scholar
Slattery, ML, Benson, J, Ma, KN, Schaffer, D & Potter, JDTransfatty acids and colon cancer. Nutr Cancer (2001) 39, 170–175.CrossRefGoogle ScholarPubMed
Sommerfeld, MTrans unsaturated fatty acids in natural products and processed foods. Prog Lipid Res (1983) 22, 221–233.CrossRefGoogle ScholarPubMed
Toomey, S, Roche, H, Fitzgerald, D & Belton, ORegression of pre-established atherosclerosis in the apoE-/- mouse by conjugated linoleic acid. Biochem Soc Trans (2003) 31, 1075–1079.CrossRefGoogle ScholarPubMed
Wang, Y & Jones, PJDietary conjugated linoleic acid and body composition. Am J Clin Nutr (2004) 79, 1153S–1158S.CrossRefGoogle ScholarPubMed
Wolff, RLContent and distribution of trans-18:1 acids in ruminant milk and meat fats. Their importance in European diets and their effect on human milk. J Am Oil Chem Soc (1995) 72, 259–272.CrossRefGoogle Scholar