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The effects of polyunsaturated:saturated fatty acids ratios and peroxidisability index values of dietary fats on serum lipid profiles and hepatic enzyme activities in rats

Published online by Cambridge University Press:  08 March 2007

Min Jeong Kang ,
Myoung Suk Shin ,
Jung Nan Park  and
Sang Sun Lee
Min Jeong Kang
Affiliation:
Department of Food and Nutrition, Hanyang University, Seoul 133-791, South Korea
Myoung Suk Shin
Affiliation:
Department of Food and Nutrition, Hanyang University, Seoul 133-791, South Korea
Jung Nan Park
Affiliation:
Department of Food and Nutrition, Hanyang University, Seoul 133-791, South Korea
Sang Sun Lee*
Affiliation:
Department of Food and Nutrition, Hanyang University, Seoul 133-791, South Korea
*
*Corresponding author: Professor S. S. Lee, fax +82 2 2281 8285, email [email protected]
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Abstract

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Raising the dietary PUFA:saturated fatty acids (SFA) ratio has been recommended for the prevention of CVD. However, a high PUFA:SFA (P:S) ratio diet enhances oxidative stress because PUFA are highly susceptible to lipid peroxidation. Thus, we focused on the role of the dietary P:S ratio and peroxidisability index (PI) value on serum and liver tissue and investigated the effects of dietary P:S ratios (0·4, 1·0, and 4·8) with a fixed PI value (81) on serum lipid parameters and hepatic enzyme activities (experiment 1). To elucidate whether those phenomena were unique to the P:S ratio, we examined the effects of dietary PI values (36, 81, 126, and 217) with a constant P:S ratio (1·0) (experiment 2). Female Sprague–Dawley rats weighing 240–280 g were fed experimental diets for 4 weeks. When dietary PI value was maintained at 81, serum HDL-cholesterol (HDL-C) increased with increasing dietary P:S ratio. When the P:S ratio was fixed at 1·0, HDL-C was the lowest with mid–low PI (MLPI) (PI value of 81). In both experiments, serum LDL-cholesterol:HDL-C ratio kept in the range of 0–2. The hepatic superoxide dismutase, catalase, and glutathione peroxidase (GSH-Px) activities and thiobarbituric acid reactive substance (TBARS) concentrations were the highest in the lowest dietary P:S ratio group (experiment 1). GSH-Px, glutathione-S-transferase, and TBARS were the lowest in rats fed the MLPI diet (experiment 2). In conclusion, these results indicate that a P:S ratio of 1·0–1·5 and a PI value of 80–90 in the diet are within a favourable range to reduce the risk of CVD.

Keywords

Dietary fatty acidsPolyunsaturated:saturated fatty acids ratioPeroxidisability indexLipid profilesAntioxidant enzyme activity
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 4 , October 2005 , pp. 526 - 532
Copyright
Copyright © The Nutrition Society 2005

References

Aebi, HE (1984) Catalase. In Methods of Enzymatic Analysis, 3red., pp.273–285 [Bergmeyer, HU, Bergmeyer, J & Graßl, M, editiors]. Weinheim, Germany: Verlag Chemie.Google Scholar
Bermingham, MA, Jones, E, Steinbeck, K & Brock, K (1995) Plasma cholesterol and other cardiac risk factors in adolescent girls. Arch Dis Child 73, 392–397.CrossRefGoogle ScholarPubMed
Bradford, MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254.Google Scholar
Buckingham, KW (1985) Effect of dietary polyunsaturated/saturated fatty acid ratio and dietary vitamin E on lipid peroxidation in the rat. J Nutr 115, 1425–1435.CrossRefGoogle ScholarPubMed
Chopra, S & Wallace, HM (1998) Induction of spermidine/spermine N1-acetyltrasferase in human cancer cells in response to increased production of reactive oxygen species. Biochem Pharmacol 55, 1119–1123.CrossRefGoogle ScholarPubMed
Claiborne, A (1984) Catalase activity. In CRC Handbook of Methods for Oxygen Radical Research, pp.283 [Greenwald, RA, editor]. Boca Raton, FLCRC Press Inc.Google Scholar
Conner, WE (2000) Importance of n -3 fatty acids in health and disease. Am J Clin Nutr 71, 171S–175S.CrossRefGoogle Scholar
Czernichow, S & Hercberg, S (2001) Interventional studies concerning the role of antioxidant vitamins in cardiovascular diseases: a review. J Nutr Health Aging 5 188–195.Google Scholar
Du, C, Sato, A, Watanabe, S, Wu, CZ, Ikemoto, A, Ando, K, Kikugawa, K, Fujii, Y & Okuyama, H (2003) Cholesterol synthesis in mice suppressed but lipofuscin formation is not affected by long-term feeding of n -3 fatty acid-enriched oils compared with lard and n -6 fatty acid-enriched oils. Biol Pharm Bull 26, 766–770.Google Scholar
Ehnholm, C, Huttunen, JK, Pietinen, P, Leino, U, Mutanen, M, Kostiainen, E, Pikkarainen, J, Dougherty, R, Iacono, J & Puska, P (1982) Effect of diet on serum lipoproteins in a population with a high risk of coronary heart disease. N Engl J Med 307, 850–855.CrossRefGoogle Scholar
Flohe, L & Gunzler, WA (1984) Assays of glutathione peroxidase. Methods Enzymol 105, 114–121.CrossRefGoogle ScholarPubMed
Fraga, CG, Leibovitz, BE & Tappel, AL (1988) Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes. Free Radic Biol Med 4, 155–161.Google Scholar
Friedewald, WT, Levy, RI & Fredrickson, DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18, 499–502.CrossRefGoogle ScholarPubMed
Green, MS, Heiss, G, Rifkind, BM, Cooper, GR, Williams, OD & Tyroler, HA (1985) The ratio of plasma high-density lipoprotein cholesterol to total and low-density lipoprotein cholesterol: age-related changes and race and sex difference in selected North American populations. The Lipid Research Clinics Program Prevalence Study. Circulation 72, 93–104.Google Scholar
Hsiang, JN, Wang, JY, Ip, SM, Ng, HK, Stadlin, A, Yu, AL & Poon, WS (1997) The time course and regional variations of lipid peroxidation after diffuse brain injury in rats. Acta Neurochir 139, 464–468.Google Scholar
Hu, ML, Frankel, EN, Leibovitz, BE & Tappel, AL (1989) Effect of dietary lipids and vitamin E on in vitro lipid peroxidation in rat liver and kidney homogenates. J Nutr 119, 1574–1582.CrossRefGoogle ScholarPubMed
Hurst, R, Bao, Y, Jemth, P, Mannervik, B & Williamson, G (1997) Phospholipid hydroperoxide glutathione peroxidase activity of rat class theta glutathione transferase T2-2. Biochem Soc Trans 25, S559.Google Scholar
Jiang, ZY, Hunt, JV & Wolff, SP (1992) Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxide in low density lipoprotein. Anal Biochem 202, 384–389.Google Scholar
Jornot, L, Petersen, H & Junod, AF (1998) Hydrogen peroxide-induced DNA damage is independent of nuclear calcium but dependent on redox-active ions. Biochem J 335, 85–94.Google Scholar
Kamanli, A, Naziroglu, M, Aydilek, N & Hacievliyagil, C (2004) Plasma lipid peroxidation and antioxidant levels in patients with rheumatoid arthritis. Cell Biochem Funct 22, 53–57.CrossRefGoogle ScholarPubMed
Kang, MJ, Lee, EK & Lee, SS (2003) Effects of P/S ratio of fatty acids and antioxidants supplement on serum lipid levels and hepatic antioxidant enzyme activities in rats. Kor J Nutr 36, 245–254.Google Scholar
Kang, MJ, Lee, EK & Lee, SS (2004) Effects of two P/S ratios with the same peroxidizability index value and antioxidants supplementation on serum lipid concentration and hepatic enzyme activities of rats. Clin Chim Acta 350, 79–87.CrossRefGoogle ScholarPubMed
Knezevic, V, Mujovic, VM & Milosevic, A (2000) Effect of vitamin E on erythrocyte enzymes and total antioxidant status in diabetic patients with ischemic heart disease. Srp Arh Celok Lek 128, 241–246.Google Scholar
Kuratko, C & Pence, BC (1991) Changes in colonic antioxidant status in rats during long-term feeding of different high fat diets. J Nutr 121, 1562–1569.CrossRefGoogle ScholarPubMed
Lands, WE, Hamazaki, T, Yamazaki, K, Okuyama, H, Sakai, K, Goto, Y & Hubbard, VS (1990) Changing dietary patterns. Am J Clin Nutr 51, 991–993.Google Scholar
Lee, JH, Fukumoto, M, Nishida, H, Ikeda, I & Sugano, M (1989) The interrelated effects of n -6/ n -3 and polyunsaturated/saturated ratios of dietary fats on the regulation of lipid metabolism in rats. J Nutr 119, 1893–1899.CrossRefGoogle Scholar
Lutoslawska, G, Tkaczyk, J, Panczenko-Kresowska, B, Hubner-Wozniak, E, Skierska, E & Gajewski, AK (2003) Plasma TBARS, blood GSH concentrations, and erythrocyte antioxidant enzyme activities in regularly menstruating women with ovulatory and anovulatory menstrual cycles. Clin Chim Acta 331, 159–163.CrossRefGoogle ScholarPubMed
Marklund, S (1984) Pyrogallol autoxidation. In CRC Handbook of Methods for Oxygen Radical Research, pp.243 [Greenwald, RA, editor]. Boca Raton, FL: CRC Press Inc.Google Scholar
Meyer, D, Liebenberg, PH & Maritz, FJ (2004) Serum lipid parameters and the prevalence of corneal arcus in a dyslipidaemic patient population. Cardiovasc J S Afr 15, 166–169.Google Scholar
Mills, EM, Takeda, K & Yu, ZX (1998) Never growth factor treatment prevents the increase in superoxide produced by epidermal growth factor in PC12 cells. J Biol Chem 273, 22165–22168.Google Scholar
Morrison, WR & Smith, LM (1964) Preparation of fatty acid methyl esters and dimethyl acetals from lipids with boron fluoride-methanol. J Lipid Res 53, 600–608.Google Scholar
Muller, H, Lindman, AS, Brantsaeter, AL & Pedersen, JI (2003) The serum LDL/HDL cholesterol ratio is influenced more favorably by exchanging saturated with unsaturated fat than by reducing saturated fat in the diet of women. J Nutr 133, 78–83.CrossRefGoogle ScholarPubMed
Nagyova, A, Krajcovicova-Kudlackova, M & Klvanova, J (2001) LDL and HDL oxidation and fatty acid composition in vegetarians. Ann Nutr Metab 45, 148–151.CrossRefGoogle ScholarPubMed
Nam, JH & Park, HS (1993) Effect of quality and quantity of dietary fats on the status of tocopherol and lipid peroxidation of plasma and tissue in rats. Kor J Nutr 26, 566–577.Google Scholar
National Research Council (1996) Guide for the Care and Use of Laboratory Animals. Bethesda, MD: National Institutes of Health.Google Scholar
Oliver, MF (1987) Dietary fat and coronary heart disease. Br Heart J 58, 423–428.Google Scholar
Park, SM, Ahn, SH, Choi, MK & Chio, SB (1999) The effect of vitamin E supplementation on insulin resistance and oxidative stress in Sprague Dawley rats fed high ω-6 polyunsaturated fat diet. Kor J Nutr 32, 644–653.Google Scholar
Qujeq, D, Aliakbarpour, HR & Kalavi, K (2004) Relationship between malondialdehyde level and glutathione peroxidase activity in diabetic rats. Clin Chim Acta 340, 79–83.CrossRefGoogle ScholarPubMed
Reeves, PG (1997) Components of the AIN-93 diets as improvements in the AIN-76A diet. J Nutr 127, 838S–841S.Google Scholar
Saito, M & Kubo, K (2003) Relationship between tissue lipid peroxidation and peroxidizability index after α-linolenic, eicosapentaenoic, or docosahexaenoic acid intake in rats. Br J Nutr 89, 19–28.Google Scholar
Warholm, M, Guthenberg, C, von Bahr, C & Mannervik, B (1985) Glutathione transferase from human liver. Methods Enzymol 113, 499–504.CrossRefGoogle ScholarPubMed