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The effects of ispaghula on rat caecal fermentation and stool output

Published online by Cambridge University Press:  09 March 2007

Christine A. Edwards ,
Jacqueline Bowen ,
W. Gordon Brydon  and
Martin A. Eastwood
Christine A. Edwards
Affiliation:
Gastrointestinal Laboratory, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU
Jacqueline Bowen
Affiliation:
Gastrointestinal Laboratory, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU
W. Gordon Brydon
Affiliation:
Gastrointestinal Laboratory, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU
Martin A. Eastwood
Affiliation:
Gastrointestinal Laboratory, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU
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Abstract

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The colonic fermentation of ispaghula, a mucilage from Plantago ovata composed mainly of arabinoxylans, and its effects on stool output and caecal metabolism were investigated. Four groups of eight rats were fed on a basal diet (45 g non-starch polysaccharides/kg) for 28 d. The diet was then supplemented with ispaghula (g/kg; 0, 5, 15 or 50) for 28 d. Ispaghula increased stool dry weight and apparent wet weight but faecal water-holding capacity (amount of water held per g dry faecal material at 0.2 mPa) was unchanged. The extent of faecal drying in the metabolism cages was measured for rats fed on the basal diet and 50 g ispaghula/kg diet. At the faecal output levels encountered, only an 8% loss of wet weight would be predicted over 24 h and this was independent of diet. Faecal short-chain fatty acid (SCFA) concentration did not change but SCFA output increased. The molar proportion of SCFA as propionic acid increased and faecal pH was reduced. Values from pooled faecal samples suggested that approximately 50% of the ingested ispaghula was excreted by the 50 g ispaghula/kg diet group. Diaminopimelic acid (a constituent of bacterial cells) concentrations fell but output was unchanged indicating no change in bacterial mass. Similar changes were seen in the caecal contents but caecal pH and SCFA were unaffected. Ispaghula increased both caecal and colonic tissue wet weight and colonic length. Our results suggest that ispaghula is partly fermented in the rat caecum and colon, and loses its water-holding capacity. However, it is still an effective stool bulker and acts mainly by increasing faecal water by some unknown mechanism

Keywords

IspaghulaPlantago ovataCaecal fermentationstool bulking
Type
Nutritional Effects of Complex Carbohydrates
Information
British Journal of Nutrition , Volume 68 , Issue 2 , September 1992 , pp. 473 - 482
Copyright
Copyright © The Nutrition Society 1992

References

REFERENCES

Anderson, J. W., Zettwoch, R. N., Feldman, T., Tietyen-Clark, J., Oeltgen, P. & Bishop, W. C. (1988). Cholesterol lowering effects of Psyllium hydrophilic mucilloid for hypercholesterolaemic men. Archives of Internal Medicine 148, 292–296.Google Scholar
Chen, W. L. & Anderson, J. W. (1984). Propionate may mediate the hypocholesterolemic effects of plant fibres in cholesterol fed rats. Proceedings of the Society for Experimental Biology and Medicine 175, 215–218.Google Scholar
Czerkawski, J. W. (1974). Methods for determining 2-6-diaminopimelic acid and 2-aminoethylphosphonic acid in gut contents. Journal of Food Science and Agriculture 25, 45–55.Google Scholar
Demigne, C., Yacoub, C. & Remesey, C. (1986). Effects of absorption of large amounts of volatile fatty acids on rat liver metabolism. Journal of Nutrition 116, 77–86.Google Scholar
Englyst, H. N. & Cummings, J. H. (1984). Simplified method for the measurement of total non-starch polysaccharides by gas-liquid chromatography of constituent sugars as alditol acetates. Analyst 109, 937–942.Google Scholar
Goodlad, R. A., Lenton, W., Ghatei, M. A., Adrian, T. E., Bloom, S. R. & Wright, N. A. (1987). Effects of an elemental diet, inert bulk and different types of dietary fibre on the response of the intestinal epithelium to refeeding in the rat and relationship to plasma gastrin, enteroglucagon and PYY concentrations. Gut 28, 171–180.Google Scholar
Goodlad, R. A., Ratcliffe, B., Fordham, J. P. & Wright, N. A. (1989). Does dietary fibre stimulate intestinal epithelial cell proliferation in germ-free rats? Gut 30, 820–825.CrossRefGoogle ScholarPubMed
Gustaffson, B. E. (1982). The physiological importance of the colonic microflora. Scandinavian Journal of Gastroenterology 77, Suppl. 77, 117–131.Google Scholar
Nyman, M. & Asp, N.-G. (1985). Bulk laxatives: their dietary fibre composition, degradation and faecal bulking capacity in the rat. Scandinavian Journal of Gastroenterology 20, 887–895.Google Scholar
Prynne, C. J. & Southgate, D. A. T. (1979). The effects of dietary fibre on faecal excretion by human subjects. British Journal of Nutrition 41, 495–503.Google Scholar
Rasmussen, H. S., Holtug, K., Andersen, J. R., Krag, E. & Mortensen, P. B. (1987). The influence of ispaghula husk and lactulose on the in vivo and in vitro production capacity of short-chain fatty acids in humans. Scandinavian Journal of Gastroenterology 22, 406–410.CrossRefGoogle ScholarPubMed
Robertson, J. A. & Eastwood, M. A. (1981). A method to measure the water holding properties of dietary fibre using suction pressure. British Journal of Nutrition 46, 247–255.CrossRefGoogle ScholarPubMed
Roediger, W. E. W. (1982). Utilization of nutrients by isolated epithelial cells of the rat colon. Gastroenterology 83, 424–429.Google Scholar
Sandhu, J. S., Hudson, G. J. & Kennedy, J. F. (1981). The gel nature and structure of the carbohydrate of ispaghula husk of Plantago ovata. Carbohydrate Research 93, 247–260.CrossRefGoogle Scholar
Sakata, T. (1987). Stimulatory effect of short-chain fatty acids on epithelial cell proliferation in the rat intestine: a possible explanation for trophic effects of fermentable fibre, gut microbes and luminal trophic factors. British Journal of Nutrition 58, 85–102.Google Scholar
Sosulski, F. W. & Cadden, A. M. (1982). Composition and physiological properties of several sources of dietary fiber. American Journal of Food Science 47, 1472–1477.CrossRefGoogle Scholar
Spiller, G. A., Chernoff, M. C., Hill, R. A., Gates, J. E., Nassar, J. J. & Shipley, E. A. (1980). Effect of purified cellulose, pectin, and a low residue diet on faecal fatty acids, transit time and faecal weight in humans. American Journal of Clinical Nutrition 33, 754–755.Google Scholar
Tomlin, J., Taylor, J. S. & Read, N. W. (1989). The effects of mixed faecal bacteria on a selection of viscous polysaccharides in vitro. Nutrition Reports International 39, 121–135.Google Scholar
Walter, D. J., Eastwood, M. A., Brydon, W. G. & Elton, R. A. (1986). An experimental design to study colonic fiber fermentation in the rat: duration of feeding. British Journal of Nutrition 55, 465–479.Google Scholar