Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-19T12:49:21.071Z Has data issue: false hasContentIssue false
  • English
  • Français

The effects of iron deficiency and iron overload on cell-mediated immunity in the mouse

Published online by Cambridge University Press:  09 March 2007

Felix O. Omara  and
Barry R. Blakley
Felix O. Omara
Affiliation:
Department of Veterinary Physiological Sciences, Western College of Veterinary Medicine, University of Saskatchewan,Saskatoon S7N OW0, Canada
Barry R. Blakley
Affiliation:
Department of Veterinary Physiological Sciences, Western College of Veterinary Medicine, University of Saskatchewan,Saskatoon S7N OW0, Canada
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The influence of Fe status on cell-mediated immunity was studied in weanling mice fed on Fe-deficient (7 mg Fe/kg), Fe-sufficient (120 mg Fe/kg) and high-Fe (3000 or 5000 mg Fe/kg) diets for 7 weeks. The contact sensitivity (CS) response to dinitrofluorobenzene (DNFB), the in vivo delayed-type hypersensitivity (DTH) response to sheep erythrocytes (SRBC) and the ability of primed spleen cells to transfer DTH response to naive normal mice were suppressed in mice consuming the Fe-deficient diet. High-Fe diets (3000 or 5000 mg Fe/kg) selectively suppressed the CS response to DNFB, but the DTH response to SRBC or the transfer of DTH response by primed spleen cells to naive normal mice remained normal. Spleen cell functions associated with the expression of class II major histocompatibility (MHC) surface antigens, concanavalin A-induced interleukin-2 (IL-2) secretion or the antigen-presenting cell (APC) ability to stimulate antigen-dependent proliferation of an SRBC-specific helper T-lymphocyte clone were not altered by Fe status. However, consistent with the suppressed DTH response in the Fe- deficient mice was the suppressed concanavalin A-induced T-lymphocyte blastogenesis and the interferon-γ (INF-γ) production by spleen cells from mice fed on the Fe-deficient diet. Spleen cells from mice fed on excess levels of Fe in the diet secreted less INF-γ than the control mice, although T- lymphocyte proliferation remained unaffected. Suppression of the cellular immune response associated with Fe deficiency may be related in part to impaired T-lymphocyte proliferation and INF-γ secretion rather than to deficits in IL-2 secretion or APC function.

Keywords

Immune functionIron deficiencyIron overload
Type
Iron Status and cell-mediated immunity
Information
British Journal of Nutrition , Volume 72 , Issue 6 , December 1994 , pp. 899 - 909
Copyright
Copyright © The Nutrition Society 1994

References

RERERENCES

Abbas, A. K., Lichtman, A. H. & Pober, J. S. (1991) Cellular and Molecular Immunology. Toronto: W. B. Saunders Company.Google Scholar
Bhan, A. K., Perry, L. L., Cantor, H., McCluskey, R. T., Benacerraf, B. & Greene, M. I. (1981) The role of T cell sets in the rejection of methylcholanthrene-induced sarcoma (S1509a) in syngeneic mice. American Journal of Pathology 102, 20–27.Google ScholarPubMed
Bhaskaram, P., Sharada, K., Sivakumar, B., Rao, K. V. & Nair, M. (1989) Effects of iron and vitamin A deficiency on macrophage functions in children. Nutrition Research 9, 35–45.CrossRefGoogle Scholar
Blakley, B. R. & Hamilton, D. L. (1988) The effect of iron deficiency on the immune response in mice. Drug-Nutrient Interactions 5, 249–255. Bretscher, P. A. (1979) In vitro induction of delayed-type hypersensitivity. European Journal of Immunology 9, 311–316.Google Scholar
Chandra, R. K. (1975) Impaired immunocompetence associated with iron deficiency. Journal of Paediatrics 86, 899–902.CrossRefGoogle ScholarPubMed
Dovrak, H. F., Galli, S. J. & Dovrak, A. M. (1986) Cellular and vascular manifestations of cell-mediated immunity. Human Pathology 17, 122–137.CrossRefGoogle Scholar
Dwyer, J., Wood, C., McNamara, J., Williams, A, Andiman, W., Rink, L., O'Conner, T. & Pearson, H. (1987) Abnormalities in immune system of children with beta thalassemia major. Clinical and Experimental Immunology 68, 621–629.Google Scholar
Flesch, I. & Kaufmann, S. H. E. (1987) Mycobacteria growth inhibition of interferon-r-activated bone marrow macrophages and differential susceptibility among strains of Mycobacterium tuberculosis. Journal of Immunology 138, 4408–4413.CrossRefGoogle ScholarPubMed
Heinzel, F. P., Sadick, M. D., Holaday, B. J., Coffman, R. M. & Locksley, J. (1989) Reciprocal expression of interferon-r or interleukin 4 during the resolution or progression of murine leishmaniasis: evidence for expansion of distinct helper T cell subsets. Journal of Experimental Medicine 169, 59–72.CrossRefGoogle ScholarPubMed
Joynson, D. H. M., Walker, D. M., Jacobs, A. & Dolby, A. E. (1972) Defect of cell mediated immunity of patients with iron deficiency anemia. Lancet ii, 1058–1059.CrossRefGoogle Scholar
Kuvibidila, S. R., Baliga, B. S. & Suskind, R. M. (1981) Effects of iron deficiency anemia on delayed cutaneous hypersensitivity in mice. American Journal of Clinical Nutrition 34, 2635–2640.CrossRefGoogle ScholarPubMed
MacDougall, L. G., Anderson, R., McNab, G. M. & Katz, J. (1975) The immune response in iron-deficient children: impaired cellular defense mechanisms with lateral humoral components. Journal of Paediatrics 86, 833–843.CrossRefGoogle Scholar
Menard, S., Colnaghi, M. & Cornalba, G. (1973) Immunogenicity and immunosensitivity of urethan-induced murine lung adenomata in relation to the host immunological impairment of the primary tumour host. British Journal of' Cancer 27, 345–350.CrossRefGoogle Scholar
Omara, F. O. & Blakley, B. R. (1993). Influence of low dietary iron and iron overload on urethan-induced lung tumours in mice. Canadian Journal of Veterinary Research 57, 209–211.Google ScholarPubMed
Ray, M. C., Tharp, M. D., Sullivan, R. J. & Tigelaar, R. E. (1983) Contact sensitivity reactions to dinitrofluorobenzene mediated by monoclonal IgE anti-DNP antibodies. Journal of Immunology 131, 1096–1102.CrossRefGoogle ScholarPubMed
Smith, K. A. (1984) Interleukin 2. Annual Review of Immunology 2, 319–333.CrossRefGoogle ScholarPubMed
Sorbie, J. & Valberg, L. S. (1975) Iron balance in the mouse. Laboratory Animal Science 24, 900–904.Google Scholar
Spear, A. T. & Sherman, A. R. (1992) Iron deficiency alters DMBA-induced tumor burden and natural killer cell cytotoxicity in rats. Journal of Nutrition 122, 46–55.CrossRefGoogle ScholarPubMed
Thorson, J. A, Smith, K. M., Gomez, F., Naumann, P. W., & Kemp, J. D. (1991) Role of iron in T cell activation: TH1 clones differ from TH2 clones in their sensitivity to inhibition of DNA synthesis caused by IgG mAbs against the transferrin receptor and the iron chelator desferrioxamine. Cellular Immunology 124, 126–137.CrossRefGoogle Scholar
Vadas, M. A, Miller, J. F., A., P., Gamble, J. & Whitelaw, A. A. (1975) A radioisotopic method to measure delayed type hypersensitivity in the mouse. International Archives of Allergy and Applied Immunology 49, 70–92.Google ScholarPubMed
Van Asbeck, B. S., Vergrugh, H. A, Van, Oost, B. A., Marx, J. J. M., Imhof, H. & Verhoef, J. (1982) Lbteriu monocytogenes meningitis and decreased phagocytosis associated with iron overload. British Medical Journal 284, 542–544.CrossRefGoogle ScholarPubMed
Van Eijk, H. H. & de Jong, G. (1992) The physiology of iron, transferrin and ferritin. Biological Trace Element Research 35, 13–24.CrossRefGoogle ScholarPubMed
Weaver, C. T. & Unanue, E. R. (1990) The costimulatory function of antigen presenting cells. Immunology Today 11. 49–55.CrossRefGoogle ScholarPubMed