Folic acid (FA) has the character of accepting and donating one-carbon units and is essential for methionine cycle and purine, pyrimidine and protein synthesis(Reference Brosnan, Macmillan and Stevens1,Reference Girard and Matte2) . However, the supply in FA based on ruminal synthesis and dietary sources is not sufficient to optimise metabolic efficiency and performance in cattle(Reference Girard and Matte2,3) . Approximately 97 % of dietary supplementary FA would be degraded by ruminal microbes(Reference Santschi, Berthiaume and Matte4). When comparing the supplementation mode at the equal addition level of FA, coated folic acid (CFA) which releases 23 % of FA in the rumen could cause more FA to reach the intestine and be absorbed compared with the supplementation of FA directly. Previous studies observed that addition of CFA elevated daily weight gain and hepatic genes expressions linked to protein synthesis metabolism in Angus bulls(Reference Liu, Chen and Wang5,Reference Wang, Liu and Zhang6) . Likewise, studies of dairy cows found that FA supply improved energy metabolism efficiency(Reference Duplessis, Girard and Santschi7), and elevated milk and milk protein yields and blood total sulphur amino acids contents(Reference Graulet, Matte and Desrochers8). In the rumen, FA was growth factor of Ruminococcus albus, R. flavefaciens and Butyrivibrio fibrisolvens (Reference Bryant and Robinson9–Reference Wejdemar11). Supplementation with CFA in bull diets elevated ruminal volatile fatty acids (VFA) production and fibrolytic micro-organism numbers and stimulated the digestion of DM, organic matter (OM), crude protein (CP) and neutral-detergent fibre (NDF) in the rumen and total tract(Reference Santschi, Berthiaume and Matte4,Reference Wang, Liu and Guo12) . The findings of studies cited above showed that dietary CFA inclusion was necessary for improving the performance and nutrients digestion in bulls.
Riboflavin (RF), as the precursor substance of FAD and FMN, participates in a variety of redox reactions involved in protein, lipid and carbohydrate metabolisms(Reference Northrop-Clewes and Thurnham13). The studies in vitro found that some strains of R. albus growth and cellulose digestion required RF(Reference Bryant and Robinson9,Reference Hall, Cheng and Burrows14) , and that B group vitamins containing RF addition stimulated protozoa growth(Reference Bonhomme15). Wu et al. observed that RF addition increased total VFA content, total bacteria, fungi and protozoa numbers and nutrients digestibility in the rumen of bulls(Reference Wu, Zhang and Wang16). However, limited response of production performance was observed in bulls with RF supply(Reference Wu, Zhang and Wang16) and in cows with a B vitamins mixture containing RF addition(Reference Majee, Schwab and Bertics17), and this might be due to the fact that 99 % of dietary-supplemented RF was degraded or metabolised in the rumen(Reference Santschi, Berthiaume and Matte4). Hence, the supplement of coated riboflavin (CRF) should be used in ruminant diets.
In the one-carbon units cycle, the FAD-dependent 5,10-methylene tetrahydrofolate reductase (MTHFR) is in charge of the reductive conversion of 5,10-methylene tetrahydrofolate into 5-methyl-tetrahydrofolate(Reference García-Minguillán, Fernandez-Ballart and Ceruelo18). Methionine synthase (MS) catalyses the reaction that homocysteine accepts the one carbon group of 5-methyl-tetrahydrofolate to convert to methionine and finally loses activity, but methionine synthase reductase (MSR) can restore MS activity via FAD/FMN reductive methylation reaction(Reference García-Minguillán, Fernandez-Ballart and Ceruelo18). Furthermore, Ganji and Kafai reported that dietary RF level was negatively related to serum homocysteine concentration, and the combined supply of FA and RF decreased serum homocysteine concentration in human(Reference Ganji and Kafai19). Therefore, RF is a key link in FA utilisation(Reference García-Minguillán, Fernandez-Ballart and Ceruelo18).
Based on the studies above, supplementing CRF in diets containing CFA might enhance the utilisation efficiency of FA. It was reported that the improvement in performance with B vitamins addition was associated with an enhancement in metabolism efficiency in bulls(Reference Liu, Chen and Wang5) or cows(Reference Duplessis, Girard and Santschi7,Reference Graulet, Matte and Desrochers8) . Hence, it was hypothesised that the increase in average daily gain (ADG) would be greater for combined addition of CFA and CRF than for CFA addition alone in bulls. This study investigated the effects of CFA or/and CRF supply on bull performance, nutrients digestion and ruminal fermentation.
Materials and methods
Animals, experimental design and diets
The animal care followed the guidelines of Animal Care and Use Committee of Shanxi Agriculture University (Taigu, Shanxi, China). This experiment was conducted from August 2020 to October 2020 at a commercial beef farm (Qixian Wanmu Beef Farm, Jinzhong, China). Forty-eight Angus bulls of 247 ± 10·9 d of age and 261 ± 3·31 kg of body weight (BW) were assigned into four treatments according to a 2 × 2 factorial and randomised block (BW) design. The CFA at 0 or 6 mg FA/kg DM was supplemented in diets including CRF 0 or 60 mg RF/kg DM. The additional level of CFA or CRF was determined based on previous studies in bulls(Reference Liu, Chen and Wang5,Reference Wang, Liu and Guo12,Reference Wu, Zhang and Wang16) . The CFA and CRF were made based on the procedures described by Wang et al.(Reference Wang, Liu and Guo12). The CFA product contains 100 g/kg of FA, 550 g/kg of hydrogenated fat (ratio of C16:0-C18:0 = 2:1), 150 g/kg of silicon dioxide, and 200 g/kg of calcium stearate. The CRF product contains 65 g/kg of RF, 460 g/kg of hydrogenated fat (ratio of C16:0-C18:0 = 2:1), 230 g/kg of silicon dioxide, and 240 g/kg of calcium stearate. The ruminal and intestinal FA or RF release percentages were measured by using four bulls with ruminal and duodenal cannulas(Reference Wang, Liu and Zhang6), and were 23 % and 67 % for CFA and 25 % and 69 % for CRF, respectively. The supplementary CFA or CRF was premixed in the bull premix. The diets shown in Table 1 were formulated based on the nutritional requirements of beef cattle in NRC(3) and served twice a day at 06.00 and 18.00 h. The experiment period consisted of 20 d adaptation and 60 d sample collection. During the trial, bulls were kept individually and consumed water and feed ad libitum.
CFA, coated folic acid; CRF, coated riboflavin; FA, folic acid; RF, riboflavin.
* Contained per kg premix: 100 mg Co, 8500 mg Cu, 50 000 mg Fe, 30 000 mg Mn, 30 000 mg Zn, 300 mg I, 300 mg Se, 7 500 000 μg vitamin A, 1200, 000 μg vitamin D and 40, 000 μg vitamin E.
† Non-fibre carbohydrate, calculated by 1000 – crude protein – neutral-detergent fibre – fat – ash.
‡ The supplementary CFA or CRF were mixed with the premix before feeding. The CFA at 0 or 6 mg FA/kg DM was supplemented in diets including CRF 0 or 60 mg RF/kg DM. The CFA product contains 100 g/kg of FA, 550 g/kg of hydrogenated fat, 150 g/kg of silicon dioxide and 200 g/kg of calcium stearate. The CRF product contains 65 g/kg of RF, 460 g/kg of hydrogenated fat, 230 g/kg of silicon dioxide and 240 g/kg of calcium stearate.
Sampling and analyses
In the sampling period, the bull BW was individually measured on day 1, 30 and 60. The bull DM intake (DMI) was determined by measuring the amount of feed offered and refusals each day. From day 50 to 56, feed and refusal samples of individual bull were collected daily. Feces of individual bull (250 g) were sampled by the rectum four times a day and with a 6-h interval. These samples were stored at −20°C, mixed by each bull in equal proportion weight (wet basis), dried at 65°C(20), and ground through a 1-mm screen. The feed, orts and feces samples were analysed for DM (method 934·01), OM (method 942·05), nitrogen (method 976·05) and acid-detergent fibre (ADF; method 973·18) by using the method of AOAC(20), and NDF by the procedure of Van Soest et al.(Reference Van Soest, Robertson and Lewis21). Acid-insoluble ash content as an indicator for digestibility was measured according to procedure described by Van-Keulen and Young(Reference Van-Keulen and Young22).
On day 57 and 58, samples of ruminal fluid for each animal was taken with a stomach tube daily at 05.00, 08.00, 11.00 and 14.00 h. To avoid saliva contamination, the first collected sample of 200 ml was abandoned and the subsequent 200 ml was kept, determined for pH, and filtered using four layers of cotton gauze. Filtrates (10 ml) used for the measurement of ammonia-N and VFA concentrations were stored at −20°C, and that (30 ml) for the determination of microbial population and enzyme activity were stored at −80°C(Reference Wang, Liu and Guo12).
Ruminal VFA concentration was analysed using a GC (GC9860; Jinan Qida Analytical Instrument Co., Ltd) and the internal standard was 2-ethylbutyric acid. Ruminal ammonia-N content was measured using a colorimetric spectrophotometer (UV-2450, Jinan Qida Analytical Instrument Co., Ltd)(20). Ruminal fluid samples were sonicated in a ice-water bath with 20-s pulse rate and 10 min and separated the supernatant by centrifuging at 30 000 g and 4°C for 10 min to measure enzyme activity according to the method of Agarwal et al.(Reference Agarwal, Kamra and Chaudhary23) and Rodrigues et al.(Reference Rodrigues, Pinto and Bezerra24). The 1·2 ml of homogenised ruminal fluid was applied to the extraction of micro-organism DNA based on the procedures (RBB + C) described by Yu and Morrison(Reference Yu and Morrison25). The extracted DNA was evaluated quality and quantity by using the agarose gel electrophoresis and NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific). Micro-organism DNA primer sequences were designed according to the reports of Zhou et al.(Reference Zhou, McSweeney and Wang26) and Stevenson and Weimer(Reference Stevenson and Weimer27) and presented in Table 2. The standard of each micro-organism DNA was produced using regular PCR, purified with the PureLinkTM Quick Gel Extraction and PCR Purification Combo Kit (Thermo Fisher Scientific Co., Ltd) and then quantified using a spectrophotometer. The copy number of micro-organism DNA standard was evaluated based on length and mass of each PCR product by using a serial 10-fold diluent from 101 to 108 DNA copies(Reference Kongmun, Wanapat and Pakdee28). The amplification and detection of real-time PCR were carried out in a Chromo 4™ system (Bio-Rad). The samples of real-time PCR amplification were analysed in triplicate by using the Biotools QuantiMix EASY SYG Kit (B&M Labs, S. A.). The PCR assay condition was initial denaturation (1 cycle of 50°C for 2 min and 95°C for 2 min) and primer annealing and product elongation (40 cycles of 95°C for 15 s and 60°C for 1 min).
On day 59, blood samples of each bull were taken via coccygeal vein using a Vacutainer system (10 ml, Jinan Qiansi Biotechnology Co. Ltd) at 09.00 h, centrifuged at 2000 g and 4°C for 15 min to separate serum and kept at −80°C. Concentrations of glucose, albumin, total protein, urea nitrogen, folate, homocysteine and RF in serum were analysed by a PT-3502PC Microplate reader (Beijing Putian Xinqiao Technology Co., Ltd) and corresponding commercial kits designed for bovine (Meilian Biotechnology Co., Ltd).
Calculation and statistical analyses
For each bull, feed efficiency (FE) was calculated as ADG divided by DMI. Supplementary CFA at 0 or 6 mg of FA/kg and CRF at 0 or 60 mg RF/kg were used in a randomised block and 2 × 2 factorial design. The MIXED procedure of SAS(29) was used to analyse the variables of current study. The data of DMI were averaged by every 30 d, and DMI, BW, ADG and FE were analysed by using the model, Yijklm = μ + Bi + G j + H k + (GH) jk + T l + (TG) jl + (TH) kl + (TGH) jkl + R m:ijk + ϵ ijklm . Rumen fluid samples were analysed using the average value of all sampling time, and nutrients digestibility and ruminal fermentation and blood parameters were analysed by using the model, Yijklm = μ + Bi + G j + H k + (GH) jk + R m:ijk + ϵ ijkm . In the model, Yijklm is the dependent variable, μ is the overall mean, B i is the random effects of the ith block, G j is the fixed effects of CFA supplementation (j = 0 or 6 mg/kg), H k is the fixed effects of CRF supplementation (k = 0 or 60 mg/kg), (GH) jk is the CFA × CRF interaction, T l is the fixed effect of time; (TG) jl is the time × CFA interaction, (TH) kl is the time × CRF interaction, (TGH) jkl is the time × CFA × CRF interaction, R m is the random effects of the mth bull, and ϵ ijklm is the the residual error. Mean separations of difference tests was done by using the PDIFF option in the LSMEANS statement (Tukey’s test) in SAS(29)only for influences that were significant at P < 0·05. The significant effects for the factors were suggested at P < 0·05.
Results
Body weight change, DM intake and feed efficiency
The significant CFA × CRF interaction was noted on ADG and FE; the increases in ADG and FE were higher (P < 0·05) for providing CRF in the CFA+ diets compared with the CFA diets (Table 3). The DMI and 0- and 30-d BW among treatments had no significant difference. The 60-d BW elevated (P < 0·05) with CFA addition and was unaltered with CRF supplementation.
FA, folic acid; RF, riboflavin; FE, feed efficiency; ADG, average daily gain.
* CFA-, CFA, CFA+, 6 mg FA/kg DM as CFA, CFR-, without CFR, CFR+, 60 mg RF/kg DM as CRF.
† CFA, CFA effect; CRF, CRF effect; CFA × CRF, the interaction between CFA and CRF addition. The P-value of time for DM intake, average daily gain and feed efficiency were 0·001, 0·001 and 0·314, respectively. The time × CFA, time × CRF and time × CFA × CRF interaction for all the studied variables were not significant (P > 0·05).
‡ FE was calculated as ADG divided by DMI.
Nutrients digestibility and ruminal fermentation
Significant CFA × CRF interaction was not observed on total tract nutrients digestibility and ruminal fermentation parameters (Table 4). The digestibility of DM, OM, CP, NDF and NFC in the total tract enhanced (P < 0·05) for CFA or CRF addition. The digestibility of ADF in the total tract elevated (P = 0·019) for CFA supply but was unaltered for CRF supply. Ruminal pH reduced (P < 0·05), but total VFA content elevated (P < 0·05) for CFA or CRF supplementation. Addition of CFA elevated (P < 0·05) acetate percentage and acetate to propionate ratio but decreased (P = 0·003) the percentage of propionate. Supplementation with CRF did not influence the percentages of acetate and propionate but elevated (P = 0·011) acetate to propionate ratio. The percentages of butyrate, valerate, isobutyrate and isovalerate were not influenced by CFA or CRF supplementation. Ammonia-N content reduced (P < 0·05) for CFA or CRF supply.
FA, folic acid; RF, riboflavin; VFA, volatile fatty acids.
* CFA-, without CFA; CFA+, 6 mg FA/kg DM as CFA; CFR-, without CFR; CFR+, 60 mg RF/kg DM as CRF.
† CFA, CFA effect; CRF, CRF effect; CFA × CRF, the interaction between CFA and CRF addition.
‡ VFA.
Rumen enzyme activity and micro-organism number
The CFA × CRF interaction was not significant for rumen enzyme activity and microbial number (Table 5). The CFA or CRF supply enhanced (P < 0·05) the activities of carboxymethyl cellulase, cellobiase and xylanase. The activities of pectinase, α-amylase and protease were enhanced (P < 0·05) by CFA but were unaffected with CRF supply. Supplementation of CFA or CRF elevated (P < 0·05) numbers of total bacteria, fungi, protozoa, R. albus, R. flavefaciens, Fibrobacter succinogenes and Prevotella ruminicola. The numbers of B. fibrisolvens and Ruminobacter amylophilus elevated (P < 0·05) for CFA supply but did not change with CRF addition.
FA, folic acid; RF, riboflavin.
* CFA- , without CFA; CFA+, 6 mg FA/kg DM as CFA; CFR-, without CFR; CFR+, 60 mg RF/kg DM as CRF.
† CFA, CFA effect; CRF, CRF effect; CFA × CRF, the interaction between CFA and CRF addition.
‡ Units of enzyme activity are carboxymethyl cellulase (μmol glucose/min/ml), cellobiase (μmol glucose/min/ml), xylanase (μmol xylose/min/ml), pectinase (μmol D-galactouronic acid/min/ml), α-amylase (μmol glucose/min/ml) and protease (μg hydrolysed protein/min/ml).
Blood parameters
Significant CFA × CRF interaction was not found for blood parameters (Table 6). Blood glucose concentration was elevated (P = 0·023) by CFA but was unaltered with CRF addition. Dietary CFA or CRF supply elevated (P < 0·05) blood contents of total protein and albumin but did not influence urea nitrogen. Blood content of folate increased (P = 0·007), homocysteine reduced (P = 0·028) and RF was unchanged for bulls with CFA supplementation. Blood concentrations of folate and RF increased (P < 0·05) and homocysteine was unaltered for bulls with CRF inclusion.
FA, folic acid; RF, riboflavin.
* CFA- , without CFA; CFA+, 6 mg FA/kg DM as CFA; CFR-, without CFR, CFR+, 60 mg RF/kg DM as CRF.
† CFA, CFA effect; CRF, CRF effect; CFA × CRF, the interaction between CFA and CRF addition.
Discussion
The CFA contains 100 g/kg of FA and CRF contains 65 g/kg of RF. The ruminal and intestinal disappearance rates were 23 % and 67 % for CFA, and 25 % and 69 % for CRF, respectively. The addition levels of CFA and CRF were 6 mg FA/kg DM and 60 mg RF/kg DM, respectively. Based on daily DMI, addition of CRF provided 126 and 123 mg of RF in the rumen and 349 and 339 mg of RF in the intestine for bulls in the CRF+ and CFA + CRF+ groups, and addition of CFA provided 11·6 and 11·3 mg of FA in the rumen and 33·9 and 32·9 mg of FA in the intestine for bulls in the CFA+ and CFA + CRF+ groups. Dietary added fat due to the coating of CFA and CRF was 0·42, 0·033 and 0·45 g/kg DM for CRF+, CFA+ and CFA + CRF+, respectively, and had limited impacts on the total fat percentage of the diets.
As in previous study of Liu et al.(Reference Liu, Chen and Wang5), CFA supplement increased ADG and FE without affecting DMI of bulls. Likewise, Levesque et al.(Reference Levesque, Girard and Matte30) reported that dietary addition of FA improved growth performance but had no effect on feed intake in calves. The changes of ADG and FE were in line with the elevation in nutrients apparent digestibility and rumen total VFA content and were likely also due to the fact that CFA supply improved metabolism efficiency of bulls. The increase in blood contents of total protein and albumin combined with the unchanged urea nitrogen content showed that dietary nitrogen utilisation efficiency might be improved with supplementation of CFA in bull diets. The increment in blood folate and reduction in blood homocysteine suggested that supplementary FA was absorbed effectively and promoted the remethylation of homocysteine to methionine, facilitating an increase in ADG. FA participates in protein synthesis metabolism by transmitting one-carbon units, and homocysteine receives the methyl group of 5-methyl-tetrahydrofolate to generate methionine(Reference Brosnan, Macmillan and Stevens1). Studies indicated that FA addition increased hepatic gene expression linked to protein synthesis metabolism in bulls(Reference Liu, Chen and Wang5), promoted murine myoblasts differentiation by activating the Akt pathway(Reference Hwang, Kang and Sung31) and improved energy metabolism efficiency in dairy cows receiving vitamin B12 addition(Reference Duplessis, Girard and Santschi7). The results of Petitclerc et al.(Reference Petitclerc, Dumoulin and Ringuet32) that intramuscular injection of FA increased weight gain of heifers during a 5-week period following weaning also indicated that metabolism efficiency might be improved by FA addition. The elevation in digestibility of DM and OM in the total tract suggested that nutrients digestibility in the rumen and post-rumen were probably enhanced by CFA supply. FA is a prime requisite for cell growth and multiplication(Reference Brosnan, Macmillan and Stevens1). Studies verified that FA addition was required for ruminal cellulose digestion in vitro (Reference Bryant and Robinson9) and improved the structure and function of small intestine in monogastric animals(Reference Davidson and Townley33,Reference Liu, Chen and Mao34) . In addition, it had been reported that digestibility of DM, CP and NDF in the rumen and total tract enhanced with CFA supplementation in steers(Reference Wang, Liu and Guo12), and DM digestibility in post-rumen and total tract elevated for FA supply in vitro (Reference Parnian-Khajehdizaj, Taghizadeh and Hosseinkhani35). Similar with the observation of Wang et al.(Reference Wang, Liu and Guo12), CFA supply reduced ruminal pH but elevated total VFA content in bulls. Nevertheless, the mean ruminal pH for bulls receiving CFA supply was 6·30 and was over the critical pH value of 6·0 for cellulolytic bacteria growth and cellulosic materials degradation(Reference Russell and Wilson36). The higher ruminal total VFA content and acetate proportion corresponded with the increase in total tract digestibility of NDF and ADF and were probably attributed to the elevation in fibrolytic enzyme (carboxymethyl cellulase, cellobiase, xylanase and pectinase) activity and total bacteria, protozoa and fungi numbers. Ruminal fibrolytic enzymes were produced by cellulolytic species (R. albus, R. flavefaciens, F. succinogenes and B. fibrisolvens), protozoa and fungi, and hydrolysed cellulosic materials to acetate(Reference Castillo-González, Burrola-Barraza and Domínguez-Viveros37). It was reported that ruminal protozoa were responsible for approximately 34 % of total microbial degradation of fibre, and fungi were capable of releasing lignin from plant particles(Reference Demeyer38,Reference Orpin39) . The present results reflected a stimulatory influence of FA on ruminal micro-organism growth and nutrients digestion. FA functions in the cycle of one-carbon units of micro-organisms(Reference Brosnan, Macmillan and Stevens1,Reference Slyter and Weaver10) . Studies verified that FA was necessary for some species of Ruminococcus and B. fibrisolvens in vitro (Reference Bryant and Robinson9–Reference Wejdemar11), and that FA supplementation increased acetate content and Lactobacillus relative abundance in the caecum of piglets(Reference Wang, Zou and Li40) as well as folate synthesis and activity of the bacteria in vitro (Reference Maynard, Cummins and Green41). The change of acetate percentage resulted in an increase in acetate to propionate ratio, causing the fermentation mode shift to more acetate yielding, reflected as the decrease in propionate percentage. Likewise, previous studies observed that CFA supply increased ruminal total VFA content, acetate proportion, microbial counts and enzymes activities in steers(Reference Wang, Liu and Guo12) or Angus bulls(Reference Liu, Chen and Wang5,Reference Wang, Liu and Zhang6) . The reduction in ruminal ammonia-N content was probably attributed to the increase in total numbers of bacteria. Rumen ammonia-N is the primary source of cellular nitrogen of bacterial species and is a predictor of conversion efficiency of feed nitrogen to microbial nitrogen(Reference Firkins, Yu and Morrison42). Moreover, studies reported that FA addition in vitro increased the utilisation efficiency of ammonia-N by bacteria(Reference Wejdemar11), and CFA supply in steer diets increased the excretion of urinary total purine derivatives, an indicator of microbial protein amount(Reference Wang, Liu and Guo12,Reference Verbic, Chen and MacLeod43) . Likewise, it was reported that ammonia-N content reduced with FA addition in vitro (Reference Parnian-Khajehdizaj, Taghizadeh and Hosseinkhani35) or in steers(Reference Wang, Liu and Guo12). The increase in activities of α-amylase and protease were in line with the increment in numbers of Rb. amylophilus, P. ruminicola and B. fibrisolvens and were a reason for the elevation of NFC and CP digestibility. Furthermore, the elevation in NFC apparent digestibility was likely the reason of the increment in blood glucose concentration, since ruminal propionate concentration (22·3 and 21·5 mM for CFA- and CFA+, respectively) was unaltered with CFA addition. Blood glucose of approximately 80 % originates from propionate gluconeogenesis, and another 20 % is absorbed from the small intestine glucose, products of NFC digestion(Reference Larsen and Kristensen44). In addition, studies in dairy cows found that FA addition upregulated genes expression related to gluconeogenesis metabolism(Reference Khan, Lei and Zhang45) and promoted utilisation efficiency of glucose precursors for gluconeogenesis when vitamin B12 was sufficient in diets(Reference Preynat, Lapierre and Thivierge46). Similarly, Duplessis et al.(Reference Duplessis, Lapierre and Pellerin47) observed that plasma glucose concentration increased for cows receiving FA supplement.
The absence of response in DMI indicated that the increase in ADG and FE with addition of CRF should be attributed to an improvement in utilisation efficiency of nutrients or/and energy. The increment in total tract nutrients digestibility and rumen total VFA content reflected that CRF supply stimulated nutrients utilisation in bulls. The higher blood RF content showed that CRF supply improved RF status of bulls. In the form of FMN or FAD, RF assists in the generation of ATP by transferring electrons(Reference Northrop-Clewes and Thurnham13), and studies indicated that RF supply improved energy-yielding metabolism in human(Reference Depeint, Bruce and Shangari48) or mice(Reference Wang, Wei and Yang49). Similarly, Wu et al.(Reference Wu, Zhang and Wang16) observed that DMI was unaltered, but ADG tended to increase linearly when RF supplementation was increased from 30, 60 to 90 mg/kg DM in Holstein bulls. Majee et al.(Reference Majee, Schwab and Bertics17) observed an unchanged feed intake and tended increased milk yield with inclusion of a B–vitamins mixture containing RF in cow diets. The apparent digestibility of DM and OM elevated for CRF addition. The elevation in content of rumen total VFA suggested that digestion of nutrients in the rumen was stimulated by CRF supply. Moreover, studies had verified that RF participated in cellular division and differentiation and was essential for maintaining normal structure and function of small intestine(Reference Nakano, Mushtaq and Heath50). Likewise, Wu et al. (Reference Wu, Zhang and Wang16) found increased ruminal and total tract digestibility of DM and OM with RF supply in Holstein bulls. The increment in CP apparent digestibility was in line with the elevation in blood contents of total protein and albumin, supporting the positive response of growth performance for bulls with CRF supply. The reduction in rumen pH (from 6·55 to 6·25) for CRF supply was consistent with the changes of total VFA content and had no negative influence on feed degradation and microbial growth(Reference Russell and Wilson36). Ruminal acetate was the principal product formed by cellulolytic bacteria, protozoa and fungi(Reference Castillo-González, Burrola-Barraza and Domínguez-Viveros37). Hence, the elevation in total VFA content and acetate to propionate ratio was related to the increase in activities of carboxymethyl cellulase, cellobiase and xylanase as well as numbers of bacteria, fungi, protozoa and fibrolytic bacteria (R. albus, R. flavefaciens and F. succinogenes), suggesting that the mode of ruminal fermentation was shifted to yield more acetate due to CRF supplementation. In the rumen, FAD accepts hydrogen ions produced in carbohydrate fermentation and transfers them to NAD to generate NADH, which participates in the reduction reaction in microbes(Reference Sepúlveda Cisternas, Salazar and García-Angulo51). Likewise, the in vitro studies found that RF supply was required for some strains of R. albus growth(Reference Bryant and Robinson9) and cellulose digestion(Reference Hall, Cheng and Burrows14), and B group vitamins containing RF addition stimulated the growth of protozoa(Reference Bonhomme15). The limited response in propionate proportion was associated with the unchanged numbers of B. fibrisolvens and Rb. amylophilus and activity of α-amylase and was in line with the unchanged blood glucose content with CRF supplementation. The current results were similar with Wu et al.(Reference Wu, Zhang and Wang16), reflecting that addition of CRF mainly favoured the growth of microbes involved in the fermentation of dietary fibre. Moreover, Beaudet et al.(Reference Beaudet, Gervais and Graulet52) proposed that starch-degrading bacteria produced more RF compared with fibre degrading microbes. The reduction in ammonia-N content was probably related to an enhancement in the synthesis of micro-organism protein. Wu et al.(Reference Wu, Zhang and Wang16) noted that dietary RF supply elevated urinary total purine derivatives excretion in bulls.
The significant CFA × CRF interaction was noted on ADG and FE, showing that the increased magnitudes of ADG and FE were higher when providing CRF in diets with CFA compared with diets without CFA. The CFA × CRF interaction was not significant on rumen total VFA content and total tract nutrients digestibility. Nevertheless, when the CFA was supplemented in diets, blood concentration of folate was higher but that of homocysteine was no difference for bulls with CRF addition compared with those without CRF addition, indicating that FA utilisation efficiency might be elevated by CRF addition. The RF is a key link in FA utilisation, and FAD and FMN are coenzymes of MTHFR and MSR(Reference García-Minguillán, Fernandez-Ballart and Ceruelo18). The MTHFR is responsible for the conversion of 5,10-methylene-tetrahydrofolate into 5-methyl-tetrahydrofolate, and MSR activates MS which catalyses homocysteine to accept the methyl group of 5-methyl-tetrahydrofolate to convert to methionine(Reference García-Minguillán, Fernandez-Ballart and Ceruelo18). Therefore, the responses of ADG and FE to CFA × CRF interaction were likely due to an increase in FA utilisation efficiency when CRF was supplemented in the CFA diets.
Conclusion
Supplementation with CFA of 6 mg FA/kg DM or CRF of 60 mg RF/kg DM promoted bull growth, and this was associated with the elevation in nutrients digestibility and ruminal VFA production. Addition of CFA or CRF stimulated growth of ruminal micro-organisms responsible for fibre degradation and changed rumen fermentation pattern to produce more acetate. The increase in ADG was greater for combined addition of CFA and CRF than for CFA or CRF addition alone, and CRF supply increased blood folate concentration in bull. Therefore, the utilisation efficiency of CFA might be enhanced with CRF addition in bull diets.
Acknowledgements
The authors thank the staff of Shanxi Agriculture University beef cattle unit for care of the animals. All authors read and approved the manuscript.
This work was supported by a grant from Key Research and Development project of Shanxi Province (201903D221001 and 201903D211012) and Animal Husbandry ‘1331 project’ Key Discipline Construction programme of Shanxi Province.
W. and Q. L. designed the experiment. J. Z., L. C. and G. G. conducted the experiment. C.-Q. X., Y.-W.Z., W.-J. H. and C.-X. P. collected and analysed data. C. W. and Q. L. wrote and revised the manuscript.
The authors declare that no conflict of interest exist.