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Effects of fish oil- and olive oil-rich diets on iron metabolism and oxidative stress in the rat

Published online by Cambridge University Press:  09 March 2007

S. Miret ,
M. P. Sáiz  and
M. T. Mitjavila
S. Miret
Affiliation:
Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal, 645, 08028 Barcelona, Spain
M. P. Sáiz
Affiliation:
Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal, 645, 08028 Barcelona, Spain
M. T. Mitjavila*
Affiliation:
Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal, 645, 08028 Barcelona, Spain
*
*Corresponding Author: Dr M. T. Mitjavila, fax +34 93 411 03 58, email [email protected]
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Abstract

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The objective of the present study was to examine the effects of fish oil (FO)- and olive oil (OO)-rich diets on Fe metabolism and oxidative stress. Rats were fed for 16 weeks with diets containing 50 g lipid/g; either OO, maize oil (MO) or FO. OO or MO diets contained a standard amount (100 m/g) of all-rac-α-tocopheryl acetate. FO diets were supplemented with 0, 100 or 200 mg all-rac-α-tocopheryl acetat/g (FO-0, FO-1 or FO-2 diets, respectively). At the end of the feeding period, we measured non-haem Fe stores in liver and spleen, and erythrocyte and reticulocyte count. We also determined antioxidants and products derived from lipid peroxidation in plasma and erythrocytes. Our results showed reduced non-haem Fe stores in rats fed any of the FO diets. Reticulocyte percentage was higher in the rats fed FO-0 and FO-1. Plasma α-tocopherol was very low in rats fed the FO-0 diet. Rats fed the FO-1 and FO-2 diets showed higher α-tocopherol in plasma than the FO-0 group but lower than the MO or OO groups. We did not observe such differences in the α-tocopherol content in erythrocyte membranes. Superoxide dismutase and glutathione peroxidase activities were lower in the erythrocytes of rats fed the FO-0 diet. The products derived from lipid peroxidation were also higher in the FO groups. The administration of FO-rich diets increased lipid peroxidation and affected Fe metabolism. On the other hand, the OO-rich diet did not increase oxidative stress and did not alter Fe metabolism. Based on these results, we conclude that FO supplementation should be advised carefully.

Keywords

Fatty acidsIron metabolismOxidative stressVitamin E
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 1 , January 2003 , pp. 11 - 18
Copyright
Copyright © The Nutrition Society 2003

References

Aebi, H (1984) Catalase in vitro. Methods in Enzymology 105, 121–126.CrossRefGoogle ScholarPubMed
Ågren, JJ, Väisänen, S, Hänninen, O, Muller, AS & Hornstra, G (1997) Hemostatic factors and platelet aggregation after fish-enriched diet or fish oil or docosahexaenoic acid supplementation. Prostaglandins, Leukotrienes and Essential Fatty Acids 57, 419–421.CrossRefGoogle ScholarPubMed
Allard, JP, Kurian, R, Aghdassi, E, Muggli, R & Royall, D (1997) Lipid peroxidation during n-3 fatty acid and vitamin E supplementation in humans. Lipids 32, 535–541.CrossRefGoogle ScholarPubMed
Bang, HO & Dyerberg, J (1972) Plasma lipids and lipoproteins in Greenlandic west coast Eskimos. Acta Medica Scandinavica 192, 85–94.Google Scholar
Bang, HO & Dyerberg, J (1987) Fatty acid pattern and ischaemic heart disease. Lancet i, 633.CrossRefGoogle Scholar
Bieri, JG, Tolliver, TJ & Catignani, GL (1979) Simultaneous determination of alpha-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography. American Journal of Clinical Nutrition 32, 2143–2149.CrossRefGoogle ScholarPubMed
Carbonell, T, Ródenas, J, Miret, S & Mitjavila, MT (1997) Fish oil and oxidative stress by inflammatory leukocytes. Free Radical Research 27, 591–597.Google Scholar
Cho, SH & Choi, YS (1994) Lipid peroxidation and antioxidant status is affected by different vitamin E levels when feeding fish oil. Lipids 29, 47–52.CrossRefGoogle ScholarPubMed
Connor, WE (2000) Importance of n-3 fatty acids in health and disease. American Journal of Clinical Nutrition 71, 171S–175S.CrossRefGoogle ScholarPubMed
Davis, BH, Ornvold, K & Bigelow, NC (1995) Flow cytometric reticulocyte maturity index: a useful laboratory parameter of erythropoietic activity in anemia. Cytometry 22, 35–39.CrossRefGoogle ScholarPubMed
Drabkin, DL & Austin, JH (1935) Spectrophotometric studies II. Preparation from washed blood cells: nitric oxide hemoglobin and sulphemoglobin. Journal of Biological Chemistry 112, 51–64.CrossRefGoogle Scholar
Drevon, CA (1992) Marine oils and their effects. Nutrition Reviews 50, 38–45.CrossRefGoogle ScholarPubMed
Eda, S, Kikugawa, K & Beppu, M (1997) Oxidatively damaged erythrocytes are recognized by membrane proteins of macrophages. Free Radical Research 27, 23–30.CrossRefGoogle ScholarPubMed
Engler, MB (1994) Vascular effects of omega-3 fatty acids: possible therapeutic mechanisms in cardiovascular disease. Journal of Cardiovascular Nursing 8, 53–67.CrossRefGoogle ScholarPubMed
Farwer, SR, der Boer, BC, Haddeman, E, Kivits, GA, Wiersma, A & Danse, BH (1994) The vitamin E nutritional status of rats fed on diets high in fish oil, linseed oil or sunflower seed oil. British Journal of Nutrition 72, 127–145.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M & Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipids from animal tissue. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle Scholar
Fujii, J & Taniguchi, N (1999) Down regulation of superoxide dismutases and glutathione peroxidase by reactive oxygen and nitrogen species. Free Radical Research 31, 301–308.CrossRefGoogle ScholarPubMed
Garrido, A, Garate, M & Valenzuela, A (1993) Changes in the antioxidant capacity of blood plasma are produced after the ingestion of high doses of fish oil. Reearch Communications in Chemical and Pathological Pharmacology 82, 367–370.Google Scholar
Haan, GJ, van der Heide, S & Wolthers, BG (1979) Analysis of fatty acids from human lipids by gas chromatography. Journal of Chromatography 162, 261–271.CrossRefGoogle ScholarPubMed
Hershko, C, Link, G & Pinson, A (1987) Modification of iron uptake and lipid peroxidation by hypoxia, ascorbic acid, and alpha-tocopherol in iron-loaded rat myocardial cell cultures. Journal of Laboratory and Clinical Medicine 110, 355–361.Google Scholar
Hu, ML (1994) Measurement of protein thiol groups and glutathione in plasma. Methods in Enzymology 233, 380–385.CrossRefGoogle ScholarPubMed
Ibrahim, W, Lee, US, Yeh, CC, Szabo, J, Bruckner, G & Chow, CK (1997) Oxidative stress and antioxidant status in mouse liver: effects of dietary lipid, vitamin E and iron. Journal of Nutrition 127, 1401–1406.CrossRefGoogle ScholarPubMed
Kawai, S, Kasashima, K & Tomita, M (1989) High-performance liquid chromatographic determination of malonaldehyde in serum. Journal of Chromatography 495, 235–238.Google Scholar
Kay, MM, Bosman, GJ, Shapiro, SS, Bendich, A & Bassel, PS (1986) Oxidation as a possible mechanism of cellular aging: vitamin E deficiency causes premature aging and IgG binding to erythrocytes. Proceedings of the National Academy of Sciences USA 83, 2463–2467.CrossRefGoogle ScholarPubMed
Kubo, K, Saito, M, Tadokoro, T & Maekawa, A (1997) Changes in susceptibility of tissues to lipid peroxidation after ingestion of various levels of docosahexaenoic acid and vitamin E. British Journal of Nutrition 78, 655–669.CrossRefGoogle ScholarPubMed
Lairon, D (1997) Dietary fatty acids and arteriosclerosis. Biomedicine and Pharmacotherapy 51, 333–336.CrossRefGoogle Scholar
Lairon, D (1999) Mediterranean diet, fats and cardiovascular disease risk: what news? British Journal of Nutrition 82, 5–6.CrossRefGoogle ScholarPubMed
Lands, WE (1992) Biochemistry and physiology of n-3 fatty acids. FASEB Journal 6, 2530–2536.CrossRefGoogle ScholarPubMed
Largillière, C & Mélancon, SB (1988) Free malondialdehyde determination in human plasma by high-performance liquid chromatography. Analytical Biochemistry 170, 123–126.CrossRefGoogle ScholarPubMed
McGuire, SO, Alexander, DW & Fritsche, KL (1997) Fish oil source differentially affects rat immune cell alpha-tocopherol concentration. Journal of Nutrition 127, 1388–1394.Google Scholar
Marklund, S (1985) Pyrogallol autooxidation. In Handbook of Methods for Oxygen Radical Research, pp. 243–247 [Greenwald, RA, editor]. Boca Raton, FL: CRC Press.Google Scholar
Moreno, JJ, Carbonell, T, Sánchez, T, Miret, S & Mitjavila, MT (2001) Olive oil decreases both oxidative stress and the production of arachidonic acid metabolites by the prostaglandin /synthase pathway in rat macrophages. Journal of Nutrition 131, 2145–2149.CrossRefGoogle ScholarPubMed
Needham, CD & Simpson, RG (1952) The benzidine test for occult blood in faeces. Quarternary Journal of Medicine 21, 123–133.Google Scholar
Perona, JS & Ruiz-Gutierrez, V (2000) Effect of two high-oleic oils on the liver lipid composition of spontaneously hypertensive rats. Life Sciences 66, 521–531.CrossRefGoogle ScholarPubMed
Pradhan, D, Weiser, M, Lumley-Sapanski, K, Frazier, D, Kemper, S, Williamson, P & Schlegel, RA (1990) Peroxidation-induced perturbations of erythrocyte lipid organization. Biochimica et Biophysica Acta 1023, 398–404.CrossRefGoogle ScholarPubMed
Prior, RL & Cao, G (1999) In vivo total antioxidant capacity: comparison of different analytical methods. Free Radical Biology and Medicine 27, 1173–1181.Google Scholar
Rabbani, PI, Sheikh, NM, Chirtel, SJ, Jackson, R & Ruffin, G (1999) Effects of long-term consumption of high doses of fish oil concentrates on clinical parameters in male and female rats. Journal of Nutritional Sciences and Vitaminology (Tokyo) 45, 553–565.CrossRefGoogle ScholarPubMed
Roche, HM (1999) Unsaturated fatty acids. Proceedings of the Nutrition Society 58, 397–401.Google Scholar
Roche, HM, Zampleas, A, Knapper, JME, Webb, D, Brooks, C, Jackson, KG, Wright, JW, Gould, BJ, Kafatos, A, Gibney, MJ & Williams, CM (1998) Effect of long-term olive oil dietary intervention on postprandial triacylglycerol and factor VII metabolism. American Journal of Clinical Nutrition 68, 552–560.CrossRefGoogle ScholarPubMed
Rodríguez, MC, Sáiz, MP, Muntané, J & Mitjavila, MT (1996) Fatty acid composition of erythrocyte membranes affects iron absorption in rats. Journal of Nutrition 126, 3109–3117.CrossRefGoogle ScholarPubMed
Ruiz-Gutiérrez, V, Pérez-Espinosa, A, Vázquez, CM & Santa-María, C (1999) Effects of dietary fats (fish, olive and high-oleic-acid sunflower oils) on lipid composition and antioxidant enzymes in rat liver. British Journal of Nutrition 82, 233–241.CrossRefGoogle ScholarPubMed
Signorini, C, Ferrali, M, Ciccoli, L, Sugherini, L, Magnani, A & Comporti, M (1995) Iron release, membrane protein oxidation and erythrocyte ageing. FEBS Letters 362, 165–170.CrossRefGoogle ScholarPubMed
Singer, P, Wirth, M, Berger, I, Heinrich, B, Godicke, W, Voigt, S, Taube, C, Jaross, W & Gehrisch, S (1992) Long-chain omega 3 fatty acids are the most effective polyunsaturated fatty acids for dietary prevention and treatment of cardiovascular risk factors. Conclusions from clinical studies. World Review of Nutrition and Dietetics 69, 74–112.CrossRefGoogle ScholarPubMed
Stocks, J & Dormandy, TL (1971) The autoxidation of human red cell lipids induced by hydrogen peroxide. British Journal of Haematology 20, 95–111.CrossRefGoogle ScholarPubMed
Sunderman, FWJ & Nomoto, S (1970) Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase activity. Clinical Chemistry 16, 903–910.CrossRefGoogle ScholarPubMed
Torrance, JD & Bothwell, TH (1968) A simple technique for measuring storage iron concentrations in formalinised liver samples. South African Journal of Medical Sciences 33, 9–11.Google ScholarPubMed
Uauy, R, Mena, P & Valenzuela, A (1999) Essential fatty acids as determinants of lipid requirements in infants, children and adults. European Journal of Clinical Nutrition 53, S66–S77.CrossRefGoogle ScholarPubMed
Uauy, R & Valenzuela, A (2000) Marine oils: the health benefits of n-3 fatty acids. Nutrition 16, 680–684.CrossRefGoogle ScholarPubMed
Van der Tempel, H, Tulleken, JE, Limburg, PC, Muskiet, FA & van Rijswijk, MH (1990) Effects of fish oil supplementation in rheumatoid arthritis. Annals of Rheumatic Diseases 49, 76–80.CrossRefGoogle ScholarPubMed
Weber, P, Bendich, A & Machlin, LJ (1997) Vitamin E and human health: rationale for determining recommended intake levels. Nutrition 13, 450–460.CrossRefGoogle ScholarPubMed
Yagi, K (1984) Assay for blood plasma or serum. Methods in Enzymology 105, 328–331.CrossRefGoogle ScholarPubMed
Yaqoob, P, Knapper, JA, Webb, DH, Williams, CM, Newsholme, EA & Calder, PC (1998) Effect of olive oil on immune function in middle-aged men. American Journal of Clinical Nutrition 67, 129–135.CrossRefGoogle ScholarPubMed