Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-09T01:31:02.491Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of disodium fumarate on in vitro rumen microbial growth, methane production and fermentation of diets differing in their forage:concentrate ratio

Published online by Cambridge University Press:  08 March 2007

R. García-Martínez ,
M. J. Ranilla ,
M. L. Tejido  and
M. D. Carro
R. García-Martínez
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071 León, Spain
M. J. Ranilla
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071 León, Spain
M. L. Tejido
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071 León, Spain
M. D. Carro*
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071 León, Spain
*
*Corresponding author: Dr M. D. Carro, fax +34 987 291311, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effects of disodium fumarate on microbial growth, CH4 production and fermentation of three diets differing in their forage content (800, 500 and 200 g/kg DM) by rumen micro-organisms in vitro were studied using batch cultures. Rumen contents were collected from four Merino sheep. Disodium fumarate was added to the incubation bottles to achieve final concentrations of 0, 4 and 8 mm-fumarate, and 15N was used as a microbial marker. Gas production was measured at regular intervals from 0 to 120 h of incubation. Fumarate did not affect (P>0·05) any of the measured gas production parameters. In 17 h incubations, the final pH and the production of acetate and propionate were increased linearly (P<0·001) by the addition of fumarate. Fumarate tended to increase (P=0·076) the organic matter disappearance of the diets and to decrease (P=0·079) the amount of NH3-N in the cultures. Adding fumarate to batch cultures tended (P=0·099) to decrease CH4 production, the mean values of the decrease being 5·4 %, 2·9 % and 3·8 % for the high-, medium- and low-forage diet, respectively. Fumarate tended to increase (P=0·082) rumen microbial growth for the high-forage diet, but no differences (P>0·05) were observed for the other two diets. These results indicate that the effects of fumarate on rumen fermentation depend on the nature of the incubated substrate, the high-forage diet showing the greatest response.

Keywords

FumarateRumen microbial growthMethaneBatch cultures
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 1 , July 2005 , pp. 71 - 77
Copyright
Copyright © The Nutrition Society 2005

References

Asanuma, N, Iwamoto, M & Hino, T (1999) Effect of the addition of fumarate on methane production by ruminal microorganisms in vitro. J Dairy Sci 82, 780–787.CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists. (1999) Official Methods of Analysis, 16th ed..5th revision Gaithersburg, MD: AOAC International.Google Scholar
Barrie, S & Workman, CT (1984) An automated analytical system for nutritional investigations using N-15 tracers. Spectr Int J 3, 439–447.Google Scholar
Bayaru, E, Kanda, S, Kamada, T, Itabashi, H, Andoh, S, Nishida, T, Ishida, M, Itoh, T, Nagara, K & Isobe, Y (2001) Effect of fumaric acid on methane production, rumen fermentation and digestibility of cattle fed roughage alone. Anim Sci J 72, 139–146.Google Scholar
Blümmel, M, Karsi, A & Russell, JR (2003) Influence of diet on growth yields of rumen micro-organisms in vitro and in vivo: influence on growth yield of variable carbon fluxed to fermentation products. Br J Nutr 90, 625–634.CrossRefGoogle ScholarPubMed
Callaway, TR & Martin, SA (1996) Effects of organic acid and monensin treatment on in vitro mixed ruminal microorganism fermentation of cracked corn. J Anim Sci 74, 1982–1989.CrossRefGoogle ScholarPubMed
Carro, MD, López, S, Valdés, C & Ovejero, FJ (1999) Effect of DL-malate on mixed ruminal microorganism fermentation using the rumen simulation technique (RUSITEC). Anim Feed Sci Technol 79, 279–288.CrossRefGoogle Scholar
Carro, MD & Miller, EL (1999) Effect of supplementing a fibre basal diet with different nitrogen forms on ruminal fermentation and microbial growth in an in vitro semicontinuous culture system (RUSITEC). Br J Nutr 82, 149–157.CrossRefGoogle Scholar
Carro, MD & Miller, EL (2002) Comparison of microbial markers ( 15 N and purine bases) and bacterial isolates for the determination of rumen microbial protein synthesis. Anim Sci 75, 315–321.CrossRefGoogle Scholar
Carro, MD & Ranilla, MJ (2003a) Influence of different concentrations of disodium fumarate on methane production and fermentation of concentrate feeds by rumen micro-organisms in vitro. Br J Nutr 90, 617–623.CrossRefGoogle ScholarPubMed
Carro, MD & Ranilla, MJ (2003b) Effect of the addition of malate on in vitro rumen fermentation of cereal grains. Br J Nutr 89, 279–288.CrossRefGoogle ScholarPubMed
Demeyer, DI & Fievez, V (2000) Ruminants et environnement: la méthanogenèse (Ruminants and environment: methanogenesis). Ann Zootech 49, 95–112.CrossRefGoogle Scholar
Demeyer, DI & Henderickx, MK (1967) Competitive inhibition of in vitro methane production by mixed rumen bacteria. Arch Int Phys Bioch 75, 157–159.Google ScholarPubMed
Dewhurst, RJ, Davies, DR & Merry, RJ (2000) Microbial protein supply from the rumen. Anim Feed Sci Technol 85, 1–21.CrossRefGoogle Scholar
France, J, Dijkstra, J, Dhanoa, MS, López, S & Bannick, A (2000) Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro: a derivation of models and other mathematical considerations. Br J Nutr 83, 143–150.CrossRefGoogle ScholarPubMed
Goering, MK & Van Soest, PJ (1970) Forage Fiber Analysis (Apparatus, Reagents, Procedures and Some Applications). Agricultural Handbook no. 379. Washington DC: Agricultural Research Services, USDA.Google Scholar
Illg, DJ & Stern, MD (1994) In vitro and in vivo comparisons of diaminopimelic acid and purines for estimating protein synthesis in the rumen. Anim Feed Sci Technol 48, 49–55.CrossRefGoogle Scholar
Isobe, Y & Shibata, F (1993) Rumen fermentation in goats administered fumaric acid. Anim Sci Technol 64, 1024–1030.Google Scholar
Iwamoto, M, Asanuma, N & Hino, T (1999) Effects of nitrate combined with fumarate on methanogenesis, fermentation, and cellulose digestion by rumen microbes in vitro. Anim Sci J 70, 471–478.Google Scholar
López, S, Newbold, CJ, Bochi-Brum, O, Moss, AR & Wallace, RJ (1999 a) Propionate precursors and other metabolic intermediates as possible alternative electron acceptors to methanogenesis in ruminal in vitro. S Afr J Anim Sci 29, 106–107.Google Scholar
López, S, Valdés, C, Newbold, CJ & Wallace, RJ (1999 b) Influence of sodium fumarate addition on rumen fermentation in vitro. Br J Nutr 81, 59–64.CrossRefGoogle ScholarPubMed
Moss, AR, Jouany, JP & Newbold, J (2000) Methane production by ruminants: its contribution to global warming. Ann Zootech 49, 231–253.CrossRefGoogle Scholar
Newbold, CJ, Ouda, JO, López, S, Nelson, N, Omed, H, Wallace, RJMoss, AR, Takahashi, J & Young, BA (2002) Propionate precursors as possible alternative electron acceptors to methane in ruminal fermentation. In Greenhouse Gases and Animal Agriculture, Proceedings, 151–154 [Takahashi, J and Young, BA, editors].Amsterdam: Elsevier.Google Scholar
Nisbet, DJ & Martin, SA (1993) Effects of fumarate, L-malate, and an Aspergillus oryzae fermentation extract on D-lactate utilization by the ruminal bacterium Selenomonas ruminantium. Curr Microbiol 26, 133–136.CrossRefGoogle Scholar
Ranilla, MJ, Carro, MD, López, S, Newbold, JC & Wallace, J (2001) Influence of N source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal micro-organisms in vitro. Br J Nutr 86, 717–724.CrossRefGoogle Scholar
Taylor, KACC (1996) A simple colorimetric assay for muramic acid and lactic acid. Appl Biochem Biotechnol 56, 49–58.CrossRefGoogle Scholar
Tejido, ML, Carro, MD, Ranilla, MJ & López, S (2001) In vitro microbial growth as affected by the type of carbohydrate and the source of N. In Proceedings of the Winter Meeting of the British Society of Animal Science, pp.152York: British Society of Animal Science.Google Scholar
Van Kessel, JAS & Russell, JB (1996) The effect of pH on ruminal methanogenesis. FEMS Microbiol Ecol 20, 205–210.CrossRefGoogle Scholar
Van Soest, PJRobertson, JB & Lewis, BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74, 3583–3597.CrossRefGoogle ScholarPubMed