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Effects of dietary protein and fat level on oxidative phosphorylation in rat heart mitochondria

Published online by Cambridge University Press:  09 March 2007

Masaaki Toyomizu  and
M. Thomas Clandinin
Masaaki Toyomizu
Affiliation:
Nutrition and Metabolism Research Group, Departments of Foods & Nutrition and Medicine, University of Alberta, Edmonton, Alberta T6G 2C2, Canada
M. Thomas Clandinin
Affiliation:
Nutrition and Metabolism Research Group, Departments of Foods & Nutrition and Medicine, University of Alberta, Edmonton, Alberta T6G 2C2, Canada
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Abstract

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The effect of dietary protein and fat levels on cardiac mitochondrial oxidative phosphorylation was assessed polarographically. Weanling rats were fed on semi-purified diets containing different protein levels (10, 30, 50 and 70%) on a gross energy basis (PGE) for 9, 23 and 58 d. Cardiac mitochondria isolated from rats fed on a 70% PGE diet for 23 d exhibited significantly reduced ADP: oxygen (ADP: O) values compared with mitochondria from rats fed on a low-protein diet. Feeding low-protein diets for 58 d increased the ADP:O value. When the dietary fat level was altered to provide (% PGE: % fat-energy): 30:14, 30:30, 70:14, 70:30, feeding 70% PGE diets reduced the ADP:O value compared with the 30 % PGE level, but no difference was observed between low-fat and high-fat groups. These results indicate that the impaired ADP:O value for rats fed on very-high-protein diets was not due to the dietary fat level but that the level of dietary protein is an important determinant of oxidative phosphorylation in rat heart mitochondria.

Keywords

Protein intakeFat intakeOxidative phosphorylationRat
Type
Metabolic Effects of Nutrition Intakes
Information
British Journal of Nutrition , Volume 69 , Issue 1 , January 1993 , pp. 97 - 102
Copyright
Copyright © The Nutrition Society 1993

References

REFERENCES

Chance, B. & Williams, G. R. (1956). The respiratory chain and oxidative phosphorylation. Advances in Enzymology 17, 65–134.Google ScholarPubMed
Clandinin, M. T. (1978). The role of dietary long chain fatty acids in mitochondrial structure and function. Effects on rat cardiac mitochondrial respiration. Journal of Nutrition 108, 273–281.Google Scholar
Clandinin, M. T. (1979). Effect of dietary long chain fatty acids on energy transport in cardiac mitochondria. FEBS Letters 102, 173–176.Google Scholar
Jaworek, B., Gruber, W. & Bergmeyer, H. U. (1974). Adenosine-5'-diphosphate and adenosine-5'-mono-phosphate. In Methods of Enzymatic Analysis, pp. 2127–2131 [Bergmeyer, H. U. editor]. New York, NY: Academic Press.CrossRefGoogle Scholar
Kita, K., Muramatsu, T. & Okumura, J. (1989). Influence of excess protein intake on whole-body protein synthesis in chicks. Nutrition Reports International 39, 1091–1097.Google Scholar
Livesey, G. (1984). The energy equivalents of ATP and the energy values of food proteins and fats. British Journal of Nutrition 51, 15–28.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–275.Google Scholar
Rafael, J., Patzelt, J., Schafer, H. & Elmadfa, I. (1984). The effect of essential fatty acid deficiency on basal respiration and function of liver mitochondria in rats. Journal of Nutrition 114, 255–262.Google Scholar
Robblee, N. M. & Clandinin, M. T. (1984). Effect of dietary fat level and polyunsaturated fatty acid content on the phospholipid composition of rat cardiac mitochondrial membranes and mitochondrial ATPase activity. Journal of Nutrition 114, 263–269.CrossRefGoogle ScholarPubMed
Statistical Analysis System Institute. SAS User's Guide: Statistics, version 6. Cary, NC, USA: SAS Institute Inc.Google Scholar
Schemmel, R. A., Stone, M., Warren, M. J. & Stoddart, K. A. (1983). Nitrogen and protein losses in rats during weight reduction with a high protein, very low energy diet or fasting. Journal of Nutrition 113, 727–734.CrossRefGoogle ScholarPubMed
Toyomizu, M., Hayashi, K., Yarnashita, K. & Tomita, Y. (1988). Response surface analyses of the effects of dietary protein on feeding and growth patterns in mice from weaning to maturity. Journal of Nutrition 118, 86–92.Google Scholar
Toyomizu, M., Matsukubo, M., Hayashi, K. & Tomita, Y. (1991). Response surface analyses of the effects of dietary fat on feeding and growth patterns in mice from weaning to maturity. Animal Production 52, 207–214.Google Scholar
Usami, M., Seino, Y., Seino, S., Takemura, J., Nakahara, H., Ideka, M. & Imura, H. (1982). Effects of high protein diet on insulin and glucagon secretion in normal rats. Journal of Nutrition 112, 681–685.Google Scholar
Wander, R. C. & Berdanier, C. D. (1985). Effects of dietary carbohydrate on mitochondrial composition and function in two strains of rats. Journal of Nutrition 115, 190–199.Google Scholar
Williams, J. N. Jr (1971). Response of oxidative phosphorylation in rat liver to prolonged protein depletion. Journal of Nutrition 101, 981–988.Google Scholar
Zaragoza, R., Renau-Piqueras, J., Portoles, M., Hernandez-Yago, J., Jorda, A. & Grisolia, S. (1987). Rats fed prolonged high protein diets show an increase in nitrogen metabolism and liver megamitochondria. Archives of Biochemistry and Biophysics 258, 426–435.CrossRefGoogle ScholarPubMed