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Effects of dietary oleic-rich oils (virgin olive and high-oleic-acid sunflower) on vascular reactivity in Wistar-Kyoto and spontaneously hypertensive rats

Published online by Cambridge University Press:  09 March 2007

M. D. Herrera ,
C. Péerez-Guerrero ,
E. Marhuenda  and
V. Ruiz-Gutiéerrez
M. D. Herrera
Affiliation:
Departamento de Farmacologíia, Facultad de Farmacia, C/Profesor García González s/n, 41012 Sevilla, Spain
C. Péerez-Guerrero
Affiliation:
Departamento de Farmacologíia, Facultad de Farmacia, C/Profesor García González s/n, 41012 Sevilla, Spain
E. Marhuenda
Affiliation:
Departamento de Farmacologíia, Facultad de Farmacia, C/Profesor García González s/n, 41012 Sevilla, Spain
V. Ruiz-Gutiéerrez*
Affiliation:
Instituto de la Grasa (CSIC), Avda, Padre García Tejero no. 4, 41012 Sevilla, Spain
*
Corresponding author: Dr Ruiz-Gutiérrez, fax +34 954 616790, email [email protected]
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Abstract

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The effects of two monounsaturated fatty acid (MUFA)-rich diets, containing virgin olive oil (OO) and high-oleic-acid sunflower oil (HOSO), on development of vascular response from isolated thoracic rat aorta and lipid composition and fatty acid composition were studied and compared with samples from rats fed on a control diet. Dietary MUFA oils were fed for 6 weeks to spontaneously hypertensive (SHR) and Wistar–Kyoto (WKY) rats from 4 weeks of age. The maximum contraction of aortic ring preparations in response to phenylephrine (10-6 M) WAS SIGNIFICANTLY DECREASED IN SHR RATS FED WITH OO (0·81 (sem 0·05) v. 1·18 (sem 0·09) g, P<0·01) and treatment with HOSO did not alter the phenylephrine-induced contractions. The relaxant responses to acetylcholine (10-5 m) were significantly enhanced (30·03 (sem 0·70) v. 18·47 (sem 0·28) %, P<0·001) in the rings from SHR rats treated with OO, and were more pronounced than in WKY rats (P<0·05). In the same way, OO attenuated the dose–response curves induced by phenylephrine (10-8–10-5 m) from SHR rats, accompained with a slower contraction. These results suggest that only the chronic feeding of OO diet was able to attenuate the vascular response of rat aorta. In addition, an increase in phospholipid content (186·7 (sd 3·2) v. 159·1 (sd 11·3) g/kg, P<0·01) and changes in the fatty acid composition of aorta (mainly a decrease in arachidonic acid) could contribute to improving endothelial function. Therefore, the effects can not be attributed exclusively to the content of MUFA (mainly oleic acid). Other components of OO, such as polyphenols, not present in HOSO, may help to explain the vascular protective effect of OO consumption.

Keywords

Olive oilHigh-oleic-acid sunflower oilVascular reactivityRat aorta
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 3 , September 2001 , pp. 349 - 357
Copyright
Copyright © The Nutrition Society 2001

References

Andriambeloson, E, Kleschyov, AL, Muller, B, Beretz, A, Stoclet, JC & Andriantsitohaina, R (1997) Nitric oxide production and endothelium-dependent vasorelaxation induced by wine polyphenols in rat aorta. British Journal of Pharmacology 120, 1053–1058.CrossRefGoogle ScholarPubMed
Andriantsitohaina, R (1999) Regulation of vascular tone by plant polyphenols: role of nitric oxide. Genetics, Physiology and Biophysics 18, Suppl. 1, 3–5.Google ScholarPubMed
Alvarez de Sotomayor, M, Pérez-Guerrero, C, Herrera, MD & Marhuenda, E (1999) Effects of chronic treatment with simvastatin on endothelial dysfunction in spontaneously hypertensive rats. Journal of Hypertension 17, 769–776.CrossRefGoogle ScholarPubMed
Arce, L, Rios, A & Valcarcel, M (1998) Determination of anticarcinogenic polyphenols present in green tea using capillary electrophoresis coupled to a flow injection system. Journal of Chromatography A 827, 113–120.CrossRefGoogle ScholarPubMed
Badley, RA, Davis, PJ & Tolley, DM (1993) Phospholipids in diagnosis. In Phospholipids Handbook, pp. 833–853 [Cevc, G, editor]. New York, NY: Marcel Dekker, Inc.Google Scholar
Bazan, E, Campbell, AK & Rapoport, RM (1993) Effects of protein kinase C activation on norepinephrine-induced phosphatidylinositide hydrolysis in intact rat aorta. European Journal of Pharmacology 245, 173–177.CrossRefGoogle ScholarPubMed
Bohr, DF & Webb, RC (1988) Vascular smooth muscle membrane in hypertension. Annual Review of Pharmacology and Toxicology 28, 389–409.CrossRefGoogle ScholarPubMed
Cevc, G (editor) (1993) Phospholipids Handbook, New York, NY: Marcel Dekker, Inc.Google Scholar
Cuevas, AM, Guasch, V, Castillo, O, Irribarra, V, Mizon, C, San Martin, A, Strobel, P, Perez, D, Germain, AM & Leighton, F (2000) A high-fat diet induces and red wine counteracts endothelial dysfunction in human volunteers. Lipids 35, 143–148.CrossRefGoogle ScholarPubMed
De Schrijver, R & Vermeulen, D (1991) Separation and quantification of phospholipids in animal tissues by Iatroscan TLC/FID. Lipids 26, 74–75.CrossRefGoogle Scholar
Egashira, K, Suzuki, S, Hirooka, Y, Kai, H, Sugimachi, M, Imaizumi, T & Takeshita, A (1995) Impaired endothelium-dependent vasodilation of large epicardial and resistance coronary arteries in patients with essential hypertension: different responses to acetylcholine and substance P. Hypertension 25, 201–206.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M & Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipides from the animal tissues. Journal of Biological Chemistry 26, 497–509.CrossRefGoogle Scholar
Garcia Regueiro, JA, Gilbert, J & Diaz, I (1994) Determination of neutral lipids from subcutaneous fat cured ham by capillary gas chromatography and liquid chromatography. Journal of Chromatography A 667, 225–233.CrossRefGoogle ScholarPubMed
Herrera, MD, Zarzuelo, A, Jiménez, J, Marhuenda, E & Duarte, J (1996) Effects of flavonoids on rat aortic smooth muscle contractility: Structure-activity relationships. Genetics and Pharmacology 27, 273–277.Google ScholarPubMed
Hirata, M, Kohse, KP, Chang, CH, Ikebe, TJ & Murad, F (1990) Mechanism of cyclic GMP inhibition of inositol phosphate formation in rat aorta segment and cultured bovine aortic smooth muscle cells. Journal of Biological Chemistry 265, 1268–1273.CrossRefGoogle ScholarPubMed
Ji, J, Benishin, CG & Pang, PK (1998) Nitric oxide selectively inhibits intracellular Ca++ release elicited by inositol trisphosphate but not caffeine in rat vascular smooth muscle. Journal of Pharmacology and Experimental Therapeutics 285, 16–21.Google Scholar
Keys, A (1995) Mediterranean diet and public health: personal reflections. American Journal of Clinical Nutrition 61, 1321S–1323S.CrossRefGoogle Scholar
Komori, S & Bolton, TB (1989) Actions of guanine nucleotides and cyclic nucleotides on calcium stores in single patch clamped smooth muscle cells from rabbit portal vein. British Journal of Pharmacology 97, 973–982.CrossRefGoogle ScholarPubMed
Kramer, JK, Blais, L, Fouchard, RC, Melnyk, RA & Kallery, KM (1997) A rapid method for the determination of vitamin E forms in tissues and diet by high-performance liquid chromatography using a normal-phase diol column. Lipids 32, 323–330.CrossRefGoogle ScholarPubMed
Massaro, M, Carluccio, MA & De Caterina, R (1999) Direct vascular antiatherogenic effects of oleic acid: a clue to the cardioprotective effects of the Mediterranean diet. Cardiologia 44, 507–513.Google Scholar
Moncada, S & Higgs, EA (1993) The L-arginine-nitric oxide pathway. New England Journal of Medicine 329, 2002–2012.Google ScholarPubMed
Moncada, S, Palmer, RMJ & Higgs, EA (1991) Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacology Reviews 43, 109–142.Google ScholarPubMed
Moncada, S, Reed, DD, Schulz, R & Palmer, RMJ (1992) Development and mechanism of a specific supersensitivity to nitrovasodilators after inhibition of vascular nitric oxide synthesis in vivo. Proceedings of the National Academy of Science, USA 88, 2166–2170.CrossRefGoogle Scholar
Monteodoro, GF, Servili, M, Baldioli, M & Miniati, E (1992) Simple and hydrolyzable phenolic compounds in virgin olive oil. Their extraction, separation and quantitative and semiquantitative evaluation by HPLC. Journal of Agriculture and Food Chemistry 40, 1571–1576.CrossRefGoogle Scholar
Muriana, FJG, Ruiz-Gutiérrez, V, Blaya, JA & Bolufer, J (1995) Phospholipid fatty acid composition of hepatopancreatic brush-border membrane vesicles fron the prawn Panaeus japonicus. Biochimie 77, 190–193.CrossRefGoogle Scholar
Navarro, MD, Hortelano, P, Periago, JL & Pita, ML (1992) Effect of dietary olive and sunflower oils on the lipid composition of the aorta and platelets and on blood eicosanoids in rats. Arteriosclerosis and Thrombosis 12, 830–835.CrossRefGoogle ScholarPubMed
Osugi, S, Shimamura, K & Sunano, S (1990) Decreased modulation by endothelium of noradrenaline-induced contractions in aorta from stroke-prone spontaneously hypertensive rats. Archives of Internal Pharmacodynamics 305, 86–99.Google ScholarPubMed
Pérez-Guerrero, C, Alvarez de Sotomayor, M, Herrera, MD & Marhuenda, E (2000) Endothelium modulates contractile response to simvastatin in rat aorta. Zeitschrift für Naturforschung 55, 121–124.CrossRefGoogle ScholarPubMed
Rapoport, RM & Murad, F (1983) Agonist-induced endothelium-dependent relaxation in rat thoracic aorta may be mediated through cyclic GMP. Circulation Research 62, 961–964.Google Scholar
Ruiz-Gutiérrez, V, Molina, MT & Vázquez, CM (1990) Comparative effects of feeding different fats on fatty acid composition of major individual phospholipids of rat hearts. Annals of Nutrition and Metabolism 34, 350–358.CrossRefGoogle ScholarPubMed
Ruiz-Gutiérrez, V, Montero, E & Villar, J (1992) Determination of fatty acid and triacylglycerol composition of human adipose tissue. Journal of Chromatography B 581, 171–178.CrossRefGoogle ScholarPubMed
Ruiz-Gutiérrez, V, Perona, JS, Pacheco, YM, Muriana, FJ & Villar, J (1999) Incorporation of dietary triacylglycerols from olive oil and high-oleic sunflower oil into VLDL triacylglycerols of hypertensive patients. European Journal of Clinical Nutrition 53, 687–693.CrossRefGoogle ScholarPubMed
Ruiz-Gutiérrez, V, Muriana, FJG, Guerrero, A, Cert, AM & Villar, J (1996) Plasma lipids, erythrocyte membrane lipids and blood pressure of hypertensive women after dietary oleic acid from two different sources. Journal of Hypertension 14, 1483–1490.CrossRefGoogle ScholarPubMed
Ruiz-Gutiérrez, V, Muriana, FJG, Maestro, R & Graciani, E (1995) Oleuropein and lipid and fatty acid composition of rat heart. Nutrition Research 15, 37–51.CrossRefGoogle Scholar
Ruiz-Gutiérrez, V, Muriana, FJG & Villar, J (1998) El aceite de oliva virgen en la prevención de enfermedades cardiovasculares perfil lipídico en plasma y composición lipídica de la membrana de eritrocito humano. Grasas y Aceites 49, 9–29.CrossRefGoogle Scholar
Ruth, P, Wang, GX, Boekhoff, I, May, B, Pfeifer, A, Penner, R, Korth, M, Breer, M & Hoffmann, F (1993) Transfected cGMP-dependent protein kinase suppresses calcium transients by inhibition of inositol 1,4,5-triphosphate production. Proceedings of the National Academy of Science, USA 90, 2623–2627.CrossRefGoogle ScholarPubMed
Shirasaki, Y, Kolm, P, Nickols, GA & Lee, TJ-F (1988) Endothelial regulation of cyclic GMP and vascular responses in hypertension. Journal of Pharmacology and Experimental Therapeutics 245, 53–58.Google ScholarPubMed
Silver, PJ, Cumiskey, WR & Harris, AL (1992) Vascular protein kinase C in Wistar-Kyoto and spontaneously hypertensive rats. European Journal of Pharmacology 212, 143–149.CrossRefGoogle ScholarPubMed
Skulladottir, G, Hardanson, T & Sigfusson, N (1985) Arachidonic acid levels in serum phospholipids of patients with angina pectoris or fatal myocardial infarction. Acta Medica Scandinavica 218, 55–58.CrossRefGoogle Scholar
Spector, AA & Yorek, MA (1985) Membrane lipid composition and cellular function. Journal of Lipid Research 26, 1015–1035.CrossRefGoogle ScholarPubMed
Sudhir, K & Angus, JA (1990) Contractile responses to alpha 1-adrenoceptor stimulation during maturation in the aorta of the normotensive and spontaneously hypertensive rat: relation to structure. Clinical and Experimental Pharmacology and Physiology 17, 69–82.CrossRefGoogle ScholarPubMed
Sulpioce, JC & Ferezou, J (1984) Squalene isolation by HPLC and quantitative comparison by HPLC and GLC. Lipids 19, 631–635.CrossRefGoogle Scholar
Sunano, S, Osugi, O & Shimamura, K (1989) Blood pressure and impairment of endothelium-dependent relaxation in spontaneously hypertensive rats. Experientia 45, 705–708.CrossRefGoogle ScholarPubMed
Taddei, S, Virdis, A, Mattei, P, Ghiadoni, L, Gennari, A, Fasolo, CB, Sudano, I & Salvetti, A (1995) Aging and endothelial function in normotensive subjects and patients with essential hypertension. Circulation 91, 1981–1987.CrossRefGoogle ScholarPubMed
Tesfamariam, B & Halpern, W (1988) Endothelium-dependent and endothelium-independent vasodilation in resistance arteries from hypertensive rats. Hypertension 11, 440–444.CrossRefGoogle ScholarPubMed
Thorin-Trescases, N, Hamilton, CA, Jardine, E & Reid, JL (1994) Signal transduction mechanisms of the vasoconstriction in hypertension. European Journal of Pharmacology 268, 199–207.CrossRefGoogle ScholarPubMed
Triggle, CR & Laher, I (1985) A review of changes in vascular smooth muscle functions in hypertension: isolated tissue versus in vivo studies. Canadian Journal of Physiology and Pharmacology 63, 355–365.CrossRefGoogle ScholarPubMed
Tsimikas, S, Philis-Tsimikas, A, Alexopoulos, S, Sigari, F, Lee, C & Reaven, PD (1999) LDL isolated from Greek subjects on a typical diet or from American subjects on an oleate-supplemented diet induces less monocyte chemotaxis and adhesion when exposed to oxidative stress. Arteriosclerosis Thrombosis and Vascular Biology 19, 122–130.CrossRefGoogle ScholarPubMed
Turla, MB & Webb, RC (1987) Enhanced vascular reactivity to protein kinase C activators in genetically hypertensive rats. Hypertension 9, III150–III154.CrossRefGoogle ScholarPubMed
Turla, MB & Webb, RC (1990) Augmented phosphoinositide metabolism in aortas from genetically hypertensive rats. American Journal of Physiology 258, H173–H178.Google ScholarPubMed
Twort, CHC & van Breemen, C (1988) Cyclic guanosine monophosphate-enhanced sequestration of Ca2+ by sarcoplasmic reticulum in vascular smooth muscle. Circulation Research 62, 961–964.CrossRefGoogle ScholarPubMed
Visioli, F & Galli, C (1994) Oleuropein protects low density lipoprotein from oxidation. Life Sciences 55, 1965–1971.CrossRefGoogle ScholarPubMed
Visioli, F & Galli, C (1995) Natural antioxidants and prevention of coronary heart disease: the potential role of olive oil and its minor constituents. Nutrition and Metabolism in Cardiovascular Disease 5, 306–314.Google Scholar