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Effects of dietary maritime pine (Pinus pinaster)-seed oil on high-density lipoprotein levels and in vitro cholesterol efflux in mice expressing human apolipoprotein A-I

Published online by Cambridge University Press:  09 March 2007

Gaëllee Asset ,
Arnaud Leroy ,
Eric Bauge ,
Robert L. Wolff ,
Jean-Charles Fruchart  and
Jean Dallongeville
Gaëllee Asset
Affiliation:
INSERM U-325, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cedex, France Département d'Athérosclérose, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cedex, France
Arnaud Leroy
Affiliation:
Laboratoire de Lipochimie Alimentaire, Universiteé Paris XI, France
Eric Bauge
Affiliation:
INSERM U-325, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cedex, France Département d'Athérosclérose, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cedex, France
Robert L. Wolff
Affiliation:
Laboratoire de Lipochimie Alimentaire, ISTAB, Universiteé Bordeaux I, France
Jean-Charles Fruchart
Affiliation:
INSERM U-325, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cedex, France Département d'Athérosclérose, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cedex, France Universiteé Lille II, 42 rue Paul Duez, 59800, Lille, France
Jean Dallongeville*
Affiliation:
INSERM U-508 and, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cedex, France Département d'Athérosclérose, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cedex, France
*
*Corresponding author: Dr Jean Dallongeville, fax +33 320 877 360, email [email protected]
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Abstract

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Maritime pine (Pinus pinaster)-seed oil contains two Δ5 unsaturated polymethylene interrupted fatty acids (all cis-5,9,12–18:3 and all cis-5,11,14–20:3 acids) one of which resembles eicosapentaenoic acid. The goal of the present study was to test whether maritime pine-seed oil consumption affects HDL and apolipoprotein (Apo) A-I levels as well as the ability of serum to promote efflux of cholesterol from cultured cells. To this end, wild type (WT) non-transgenic mice and transgenic mice expressing human ApoA-I (HuA-ITg) were fed on isoenergetic diet containing either 200 g maritime pine-seed oil/kg or 200 g lard/kg for 2 weeks. WT and HuA-ITg mice fed maritime pine-seed oil had lower cholesterol, HDL-cholesterol, LDL-cholesterol and HuA-ITg mice had lower human ApoA-I than those fed lard. The differences in cholesterol (P < 0·0001) and HDL-cholesterol (P < 0·003) levels between mice fed on the two diets were more pronounced in the HuA-ITg than in the WT mice. The ability of HuA-ITg serum to promote cholesterol efflux in cultured cells was greater (P < 0·008) than that of WT animals. However, the maritime pine-seed oil diet was associated with lower (P < 0·005) in vitro cholesterol efflux ability than the lard diet in both mice genotypes. This suggests a negative effect of the maritime pine-seed oil on reverse cholesterol transport. Cholesterol efflux was correlated with serum free or esterified cholesterol and phospholipid levels. The slope of the regression line was smaller in the HuA-ITg than in the WT mice indicating that overexpression of human ApoA-I reduces the negative impact of maritime pine-seed oil on cholesterol efflux. In conclusion, maritime pine-seed oil diet lowers HDL-cholesterol and diminishes in vitro cholesterol efflux. This potentially detrimental effect is attenuated by overexpression of human ApoA-I in mice.

Keywords

Maritime pine-seed oilApolipoprotein A-ILipoproteinsReverse cholesterol transport
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 3 , September 2000 , pp. 353 - 360
Copyright
Copyright © The Nutrition Society 2000

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