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Effects of dietary macronutrient content on energy metabolism and uncoupling protein mRNA expression in broiler chickens

Published online by Cambridge University Press:  09 March 2007

Anne Collin ,
Ramon D. Malheiros ,
Vera M. B. Moraes ,
Pieter Van As ,
Veerle M. Darras ,
Mohammed Taouis ,
Eddy Decuypere  and
Johan Buyse
Anne Collin
Affiliation:
Station de Recherches Avicoles, Institut National de la Recherche Agronomique, F-37380 Nouzilly, France Laboratory for Physiology of Domestic Animals, Department of Animal Production, K. U. Leuven, Kasteelpark Arenberg 30, B-3001 Leuven, Belgium
Ramon D. Malheiros
Affiliation:
Laboratory for Physiology of Domestic Animals, Department of Animal Production, K. U. Leuven, Kasteelpark Arenberg 30, B-3001 Leuven, Belgium
Vera M. B. Moraes
Affiliation:
Laboratory for Physiology of Domestic Animals, Department of Animal Production, K. U. Leuven, Kasteelpark Arenberg 30, B-3001 Leuven, Belgium UNESP, Campus de Jaboticabal, São Paulo, Brazil
Pieter Van As
Affiliation:
Laboratory for Physiology of Domestic Animals, Department of Animal Production, K. U. Leuven, Kasteelpark Arenberg 30, B-3001 Leuven, Belgium
Veerle M. Darras
Affiliation:
Laboratory of Comparative Endocrinology, K. U. Leuven, Naamsestraat 61, B-3000 Leuven, Belgium
Mohammed Taouis
Affiliation:
Station de Recherches Avicoles, Institut National de la Recherche Agronomique, F-37380 Nouzilly, France
Eddy Decuypere
Affiliation:
Laboratory for Physiology of Domestic Animals, Department of Animal Production, K. U. Leuven, Kasteelpark Arenberg 30, B-3001 Leuven, Belgium
Johan Buyse*
Affiliation:
Laboratory for Physiology of Domestic Animals, Department of Animal Production, K. U. Leuven, Kasteelpark Arenberg 30, B-3001 Leuven, Belgium
*
*Corresponding author: Dr Johan Buyse, fax +32 16 321994, email [email protected]
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Abstract

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The objective of the present study was to investigate the effects of dietary macronutrient ratio on energy metabolism and on skeletal muscle mRNA expression of avian uncoupling protein (UCP), thought to be implicated in thermogenesis in birds. Broiler chickens from 2 to 6 weeks of age received one of three isoenergetic diets containing different macronutrient ratios (low-lipid (LL) 30 v. 77 g lipid/kg; low-protein (LP) 125 v. 197 g crude protein (N×6·25)/kg; low-carbohydrate (LC) 440 v. 520 g carbohydrate/kg). LP chickens were characterised by significantly lower body weights and food intakes compared with LL and LC chickens (−47 and −38 % respectively) but similar heat production/kg metabolic body weight, as measured by indirect calorimetry, in the three groups. However, heat production/g food ingested was higher in animals receiving the LP diet (+41 %, P<0·05). These chickens also deposited 57 % less energy as protein (P<0·05) and 33 % more as fat. No significant differences in energy and N balances were detected between LL and LC chickens. The diets with the higher fat contents (i.e. the LP and LC diets) induced slightly but significantly higher relative expressions of avian UCP mRNA in gastrocnemius muscle, measured by reverse transcription–polymerase chain reaction, than the LL diet (88 and 90 v. 78 % glyceraldehyde-3-phosphate dehydrogenase respectively, P<0·05). Our present results are consistent with the recent view that UCP homologues could be involved in the regulation of lipid utilisation as fuel substrate and provide evidence that the macronutrient content of the diet regulates energy metabolism and especially protein and fat deposition.

Keywords

MacronutrientsEnergy metabolismUncoupling proteinsBroiler chickens
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 2 , August 2003 , pp. 261 - 269
Copyright
Copyright © The Nutrition Society 2003

References

Boss, O, Samec, S & Kühne, F (1998) Uncoupling protein-3 expression in rodent skeletal muscle is modulated by food intake but not by changes in environmental temperature. J Biol Chem 273, 5–8.CrossRefGoogle Scholar
Buyse, J, Decuypere, E, Berghman, L, Kühn, ER & Vandesande, F (1992) The effect of dietary protein content on episodic growth hormone secretion and on heat production of male broilers. Br Poult Sci 33, 1101–1109.CrossRefGoogle Scholar
Buyse, J, Janssens, GP & Decuypere, E (2001) The effects of dietary L-carnitine supplementation on the performance, organ weights and circulating hormone and metabolite concentrations of broiler chickens reared under a normal or low temperature schedule. Br Poult Sci 42, 230–241.CrossRefGoogle ScholarPubMed
Buyse, J, Michels, H & Vloeberghs, J et al. (1998) Energy and protein metabolism between 3 and 6 weeks of age of male broiler chickens selected for growth rate for improved food efficiency. Br Poult Sci 39, 264–272.CrossRefGoogle ScholarPubMed
Carew, LB & Alster, FA (1997) Dietary carbohydrate and fat do not alter the thyroid response to protein deficiency in chicks. Proc Soc Exp Biol Med 215, 82–86.CrossRefGoogle Scholar
Collin, A, Buyse, J & Van As, P (2003) Cold-induced enhancement of avian uncoupling protein expression, heat production and triiodothyronine concentrations in broiler chicks. Gen Comp Endocrinol 130, 70–77.CrossRefGoogle ScholarPubMed
Darras, VM, Visser, TJ, Berghman, LR & Kuhn, ER (1992) Ontogeny of type I and III deiodinase activities in embryonic and posthatch chicks: relationship with changes in plasma triiodothyronine and growth hormone levels. Comp Biochem Physiol 103, 131–136.CrossRefGoogle ScholarPubMed
Donaldson, WE (1985) Lipogenesis and body fat in chicks: Effect of calorie:protein ratio and dietary fat. Poult Sci 64, 191–204.CrossRefGoogle ScholarPubMed
Dulloo, AG & Samec, S (2001) Uncoupling proteins: their roles in adaptive thermogenesis and substrate metabolism reconsidered. Br J Nutr 86, 123–139.CrossRefGoogle ScholarPubMed
Ferrannini, E (1988) The theoretical bases of indirect calorimetry: a review. Metabolism 37, 287–301.CrossRefGoogle ScholarPubMed
Gabarrou, J-F, Geraert, P-A, Picard, M & Bordas, A (1997) Diet-induced thermogenesis in cockerels is modulated by genetic selection for high or low residual feed intake. J Nutr 127, 2371–2376.CrossRefGoogle ScholarPubMed
Geraert, PA, MacLeod, MG & Leclercq, B (1988) Energy metabolism in genetically fat and lean chickens: diet- and cold-induced thermogenesis. J Nutr 118, 1232–1239.CrossRefGoogle ScholarPubMed
Jones, R & Smith, WK (1986) Effect of dietary protein concentration on the growth and carcass composition of male broilers from hatch to maturity. Br Poult Sci 27, 502–503.Google Scholar
Kita, K, Muramatsu, T & Okumura, J (1993) Effect of dietary protein and energy intakes on whole-body protein turnover and its contribution to heat production in chicks. Br J Nutr 69, 681–688.CrossRefGoogle ScholarPubMed
Lin, CY, Friars, GW & Moran, ET (1980) Genetic and environmental aspects of obesity in broilers. Worlds Poult Sci J 36, 103–111.CrossRefGoogle Scholar
MacLeod, MG (1990) Energy and nitrogen intake, expenditure and retention at 20° in growing fowl given diets with a wide range of energy and protein contents. Br J Nutr 64, 625–637.CrossRefGoogle ScholarPubMed
MacLeod, MG (1992) Energy and nitrogen intake, expenditure and retention at 32° in growing fowl given diets with a wide range of energy and protein contents. Br J Nutr 67, 195–206.CrossRefGoogle ScholarPubMed
Masaki, T, Yoshimatsu, H & Sakata, T (2000) Expression of rat uncoupling protein family mRNA levels by chronic treatment with thyroid hormone. Int J Obes Relat Metab Disord 24, S162–S164.CrossRefGoogle ScholarPubMed
Matsuda, J, Hosoda, K & Itoh, H (1997) Cloning of rat uncoupling protein-3 and uncoupling protein-2 cDNAs: their gene expression in rats fed high-fat diet. FEBS Lett 418, 200–204.CrossRefGoogle ScholarPubMed
Nieto, R, Aguilera, JF, Fernandez-Figares, I & Prieto, C (1997) Effect of a low protein diet on the energy metabolism of growing chickens. Arch Tierernahr 50, 105–109.CrossRefGoogle ScholarPubMed
Raimbault, S, Dridi, S & Denjean, F (2001) An uncoupling protein homologue putatively involved in facultative thermogenesis in birds. Biochem J 353, 441–444.CrossRefGoogle ScholarPubMed
Reyns, GE, Janssens, KA, Buyse, J, Kühn, ER & Darras, VM (2002) Changes in thyroid hormone levels in chicken liver during fasting and refeeding. Comp Biochem Physiol B 132, 239–245.CrossRefGoogle ScholarPubMed
Ricquier, D & Bouillaud, F (2000) The uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP. Biochem J 345, 161–179.CrossRefGoogle ScholarPubMed
Romijn, C & Lokhorst, W (1961) Some aspects of energy metabolism in birds. In Proceedings of the IInd Symposium on Energy Metabolism. European Association for Animal Production. pp. 49–59. [Brouwer, E and Van Es, EJH, editors]. Lunteren: European Association for Animal Production.Google Scholar
Rosebrough, RW, McMurtry, JP & Vasilatos-Younken, R (1999) Dietary fat and protein interactions in broilers. Poult Sci 78, 992–998.CrossRefGoogle Scholar
Rosebrough, RW, Mitchell, AD & McMurtry, JP (1996) Dietary crude protein changes rapidly alter metabolism and plasma insulin-like growth factor-I concentrations in broiler chickens. J Nutr 126, 2888–2898.Google ScholarPubMed
Rosebrough, RW & Steele, NC (1985) Energy and protein relationships in the broiler. 1. Effect of protein levels and feeding regimens on growth, body composition, and in vitro lipogenesis of broiler chicks. Poult Sci 64, 119–126.CrossRefGoogle ScholarPubMed
Scarpace, PJ, Nicolson, M & Matheny, M (1998) UCP2, UCP3 and leptin gene expression: modulation by food restriction and leptin. J Endocrinol 159, 349–357.CrossRefGoogle ScholarPubMed
Stofflet, ES, Koebel, DD, Sakar, G & Sommer, SS (1988) Genomic amplification with transcript sequenceing. Science 239, 491–494.CrossRefGoogle Scholar
Stubbs, RJ, Prentice, AM & James, WPT (1997) Carbohydrates and energy balance. Ann NY Acad Sci 819, 44–69.CrossRefGoogle ScholarPubMed
Tanaka, K, Ohtani, S & Shigeno, K (1983) Effect of increasing dietary energy on hepatic lipogenesis in growing chickens. II. Increasing energy by fat or protein supplementation. Poult Sci 62, 452–458.CrossRefGoogle ScholarPubMed
Tsuboyama-Kasaoka, N, Takahashi, M, Kim, H & Ezaki, O (1999) Up-regulation of liver uncoupling protein-2 mRNA by either fish oil feeding or fibrate administration in mice. Biochem Biophys Res Commun 257, 879–885.CrossRefGoogle ScholarPubMed
Yeh, YY & Leveille, GA (1969) Effect of dietary protein on hepatic lipogenesis in the growing chick. J Nutr 98, 356–366.CrossRefGoogle ScholarPubMed
Znaniecka, G (1967) Calorific value of protein and fat in the chicken's body. In Proceedings of the IVth European Association for Animal Production Symposium on the Energy Metabolism of Farm Animals, pp. 407–408. [Blaxter, KL, Kielanowski, J & Thorbek, G, editors]. Newcastle-upon-Tyne: Oriel Press.Google Scholar