Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-15T17:14:19.948Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of dietary lipids on immune function in a murine sensitisation model

Published online by Cambridge University Press:  09 March 2007

Ruud Albers ,
Marianne Bol ,
Astrid Willems ,
Cor Blonk  and
Raymond Pieters
Ruud Albers
Affiliation:
Unilever Health Institute, P.O. Box 114, 3130 AC Vlaardingen, The Netherlands
Marianne Bol
Affiliation:
IRAS-Immunotoxicology, Utrecht University P.O. Box 80176, 3508 TD Utrecht,The Netherlands
Astrid Willems
Affiliation:
Unilever Health Institute, P.O. Box 114, 3130 AC Vlaardingen, The Netherlands
Cor Blonk
Affiliation:
Unilever Health Institute, P.O. Box 114, 3130 AC Vlaardingen, The Netherlands
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

We have tested the effect of dietary fatty acids on aspects of innate and specific adaptive T helper (Th) 1- and Th2-driven immune responses in a murine sensitisation model using dinitrochlorobenzene as sensitiser. Six groups of fifteen BALB/c mice were fed diets containing 30 % fat (by energy) for 8 weeks. Diets were rich in saturated fatty acids, n-6 polyunsaturated fatty acid (PUFA), or n-3 PUFA, each at a sufficient (11, 35 and 68 mg/kg) and a supplemented vitamin E level (1028, 1031 and 1030 mg/kg respectively). Feeding n-6 PUFA marginally decreased % phagocytosing cells at the low vitamin E level, but had no other effects on immune function. The n-3 PUFA diets decreased production of prostaglandin E2 while increasing oxidative burst and tumour necrosis factor α production. In addition adaptive Th1-driven responses (immunoglobulin, Ig)G2a, IgG2b, interferon-γ:interleukin 4) were decreased, whereas Th2-driven and mucosal immune responses were increased (IgE) or unaffected (IgG1, IgA). Combination with high levels of α-tocopherol did not affect the reduced prostaglandin E2 production, augmented the increase of tumour necrosis factor α production and tended to ameliorate the selective suppressive effects of n-3 PUFA on certain Th1-driven effects (interferon-γ:interleukin 4 ratio and IgG2a levels). We conclude that the sensitisation model appears useful for application in nutrition research. It allows a broad assessment of the effects of dietary intervention on various aspects of immune responsiveness, and as such provides a valuable model to assess, characterise and rank effects of foods and/or nutrients on a range of immune functions, including Th1–Th2 polarisation.

Keywords

ImmunomodulationAnimal modelFatty acidsVitamin E
Type
Research Article
Information
British Journal of Nutrition , Volume 88 , Issue 3 , September 2002 , pp. 291 - 299
Copyright
Copyright © The Nutrition Society 2002

References

Albers, R, de Heer, C, Bol, M, Bleumink, R, Seinen, W & Pieters, R (1998) Selective immunomodulation by the autoimmunity-inducing xenobiotics streptozotocin and HgCl2. European Journal of Immunology 28, 1233–1242.3.0.CO;2-G>CrossRefGoogle ScholarPubMed
Bayer, EA & Wilchek, M (2001) The use of avidin biotin complex as a tool in molecular biology. Methods in Biochemical Analysis 26, 1–45.Google Scholar
Bendich, A (1999) Role of antioxidants in the maintenance of immune functions. In Natural Antioxidants in Human Health and Disease, pp. 447–467 [Frei, B, editor]. New York: Academic Press.Google Scholar
Black, PN & Sharpe, S (1997) Dietary fat and asthma: is there a connection? European Respiratory Journal 10, 6–12.CrossRefGoogle ScholarPubMed
Blok, WL, de Bruijn, MF, Leenen, PJ, Eling, WM, Van Rooijen, N, Stanley, ER, Buurman, WA & van der Meer, JW (1996) Dietary n-3 fatty acids increase spleen size and postendotoxin circulating TNF in mice; role of macrophages, macrophage precursors, and colony-stimulating factor-1. Journal of Immunology 157, 5569–5573.CrossRefGoogle ScholarPubMed
Bronson, R, Birt, D & Meydani, SN (1999) Biomarkers as early predictors of long-term health status and human immune function. Nutrition Reviews 57, S7–S12.CrossRefGoogle ScholarPubMed
Calder, PC (1997) n-3 Polyunsaturated fatty acids and cytokine production in health and disease. Annals of Nutrition and Metabolism 41, 203–234.CrossRefGoogle ScholarPubMed
Calder, PC (1998) Dietary fatty acids and the immune system. Nutrition Reviews 56, S70–S83.CrossRefGoogle ScholarPubMed
Chandra, RK (1997) Nutrition and the immune system: an introduction. American Journal of Clinical Nutrition 66, 460S–463S.CrossRefGoogle ScholarPubMed
Cher, DJ & Mosmann, TR (1987) Two types of murine helper T cell clone. II Delayed-type hypersensitivity is mediated by TH1 clones. Journal of Immunology 138, 3688–3694.CrossRefGoogle ScholarPubMed
Dewille, JW, Fraker, PJ & Romsos, DR (1981) Effects of dietary fatty acids on delayed-type hypersensitivity in mice. Journal of Nutrition 111, 2039–2043.CrossRefGoogle ScholarPubMed
Fritsche, KL, Byrge, M & Feng, C (1999) Dietary omega-3 polyunsaturated fatty acids from fish oil reduce interleukin-12 and interferon-gamma production in mice. Immunology Letters 65, 167–173.CrossRefGoogle ScholarPubMed
Haby, MM, Peat, JK, Marks, CB, Woolcock, AJ & Leeder, SR (2001) Asthma in preschool children: prevalence and risk factors. Thorax 56, 589–595.CrossRefGoogle ScholarPubMed
Han, SN, Meydani, M, Wu, D, Bender, BS, Smith, DE, Vina, J, Cao, G, Prior, RL & Meydani, SN (2000 a) Effect of long-term dietary antioxidant supplementation on influenza virus infection. Journal of Gerontology 55, B496–B503.Google ScholarPubMed
Han, SN, Wu, D, Ha, WK, Beharka, A, Smith, DE, Bender, BS & Meydani, SN (2000 b) Vitamin E supplementation increases T helper 1 cytokine production in old mice infected with influenza virus. Immunology 100, 487–493.CrossRefGoogle ScholarPubMed
Hayek, MG, Taylor, SF, Bender, BS, Han, SN, Meydani, M, Smith, DE, Eghtesada, S & Meydani, SN (1997) Vitamin E supplementation decreases lung virus titers in mice infected with influenza. Journal of Infectious Diseases 176, 273–276.CrossRefGoogle ScholarPubMed
Hinds, A & Sanders, TA (1993) The effect of increasing levels of dietary fish oil rich in eicosapentaenoic and docosahexaenoic acids on lymphocyte phospholipid fatty acid composition and cell-mediated immunity in the mouse. British Journal of Nutrition 69, 423–429.CrossRefGoogle Scholar
Jeffery, NM, Sanderson, P, Sherrington, EJ, Newsholme, EA & Calder, PC (1996) The ratio of n-6 to n-3 polyunsaturated fatty acids in the rat diet alters serum lipid levels and lymphocyte functions. Lipids 31, 737–745.CrossRefGoogle ScholarPubMed
Jolly, CA & Fernandes, G (1999) Diet modulates Th-1 and Th-2 cytokine production in the peripheral blood of lupus-prone mice. Journal of Clinical Immunology 19, 172–178.CrossRefGoogle ScholarPubMed
Lokesh, BR, Sayers, TJ & Kinsella, JE (1990) Interleukin-1 and tumor necrosis factor synthesis by mouse peritoneal macrophages is enhanced by dietary n-3 polyunsaturated fatty acids. Immunology Letters 23, 281–285.CrossRefGoogle ScholarPubMed
Meydani, SN & Beharka, AA (1998) Recent developments in vitamin E and immune response. Nutrition Reviews 56, S49–S58.CrossRefGoogle ScholarPubMed
Moriguchi, S, Kobayashi, N & Kishino, Y (1990) High dietary intakes of vitamin E and cellular immune functions in rats. Journal of Nutrition 120, 1096–1102.CrossRefGoogle ScholarPubMed
Muggli, R (1994) Physiological requirements of vitamin E as a function of the amount and type of polyunsaturated fatty acid. World Review of Nutrition and Dietetics 75, 166–168.CrossRefGoogle ScholarPubMed
Pieters, R & Albers, R (1999) Assessment of autoimmunogenic potential of xenobiotics using the popliteal lymph node assay. Methods 19, 71–77.CrossRefGoogle ScholarPubMed
Prickett, JD, Robinson, DR & Bloch, KJ (1982) Enhanced production of IgE and IgG antibodies associated with a diet enriched in eicosapentaenoic acid. Immunology 46, 819–826.Google ScholarPubMed
Reeves, PG, Nielsen, FH & Fahey, GC (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. Journal of Nutrition 123, 1939–1951.CrossRefGoogle Scholar
Sanderson, P, Yaqoob, P & Calder, PC (1995) Effects of dietary lipid manipulation upon graft vs host and host vs graft responses in the rat. Cellular Immunology 164, 240–247.CrossRefGoogle ScholarPubMed
Tanaka, J, Fujiwara, H & Torisu, M (1979) Vitamin E and immune response. I Enhancement of helper T cell activity by dietary supplementation of vitamin E in mice. Immunology 38, 727–734.Google ScholarPubMed
Tijburg, LB, Haddeman, E, Kivits, GA, Weststrate, JA & Brink, EJ (1997) Dietary linoleic acid at high and reduced dietary fat level decreases the faecal excretion of vitamin E in young rats. British Journal of Nutrition 77, 327–336.Google ScholarPubMed
Tomobe, YI, Morizawa, K, Tsuchida, M, Hibino, H, Nakano, Y & Tanaka, Y (2000) Dietary docosahexaenoic acid suppresses inflammation and immunoresponses in contact hypersensitivity reaction in mice. Lipids 35, 61–69.CrossRefGoogle ScholarPubMed
van't Erve, EH, Wijnand, E, Bol, M, Seinen, W & Pieters, RH (1998) The vehicle modulates cellular and humoral responses in contact hypersensitivity to oxazolone. Toxicological Sciences 44, 39–45.CrossRefGoogle ScholarPubMed
Van Zijverden, M, van der, PA, Bol, M, Van Pinxteren, FA, de Haar, C, Penninks, AH, Van Loveren, H & Pieters, R (2000) Diesel exhaust, carbon black, and silica particles display distinct Th1/Th2 modulating activity. Toxicology and Applied Pharmacology 168, 131–139.CrossRefGoogle ScholarPubMed
Wallace, FA, Miles, EA & Calder, PC (2000) Activation state alters the effect of dietary fatty acids on pro-inflammatory mediator production by murine macrophages. Cytokine 12, 1374–1379.CrossRefGoogle ScholarPubMed
Wallace, FA, Miles, EA, Evans, C, Stock, TE, Yaqoob, P & Calder, PC (2001) Dietary fatty acids influence the production of Th1- but not Th2-type cytokines. Journal of Leukocyte Biology 69, 449–457.CrossRefGoogle Scholar