Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-24T02:02:06.204Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of dietary coconut oil on apolipoprotein B synthesis and VLDL secretion by calf liver slices

Published online by Cambridge University Press:  09 March 2007

Dominique Gruffat-mouty ,
Benoît Graulet ,
Denys Durand ,
Marie Elisabeth Samson-Bouma  and
Dominique Bauchart
Dominique Gruffat-mouty*
Affiliation:
Unité de Recherches sur les Herbivores, Équipe Nutriments et Métabolismes, Institut National de Recherche Agronomique, Centre de Recherches Clermont Ferrand - Theix, 63122 Saint Genès Champanelle, France
Benoît Graulet
Affiliation:
Unité de Recherches sur les Herbivores, Équipe Nutriments et Métabolismes, Institut National de Recherche Agronomique, Centre de Recherches Clermont Ferrand - Theix, 63122 Saint Genès Champanelle, France
Denys Durand
Affiliation:
Unité de Recherches sur les Herbivores, Équipe Nutriments et Métabolismes, Institut National de Recherche Agronomique, Centre de Recherches Clermont Ferrand - Theix, 63122 Saint Genès Champanelle, France
Marie Elisabeth Samson-Bouma
Affiliation:
INSERM U 327, Faculté de Médecine Xavier Bichat, 16 rue Henri Huchard, BP 416, 75870 Paris Cedex 18, France
Dominique Bauchart
Affiliation:
Unité de Recherches sur les Herbivores, Équipe Nutriments et Métabolismes, Institut National de Recherche Agronomique, Centre de Recherches Clermont Ferrand - Theix, 63122 Saint Genès Champanelle, France
*
*Corresponding author: Dr Dominique Gruffat-Mouty, fax +33 04 73 62 46 39, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Incorporation of coconut oil (CO) rich in lauric acid into the milk diet induces a lipid infiltration of the liver (steatosis) in 1-month-old calves. Among possible steps involved in diet-induced liver steatosis, the ability of the calf liver to synthesize apolipoprotein (Apo) B and to secrete it as part of VLDL particles was investigated. Liver samples were taken from calves fed for 17 d on a conventional milk replacer containing CO (n 5) and beef tallow (BT, n 4) as reference. Samples were cut into slices 0·5 mm thick and subsequently incubated for 12 h in a medium containing a [35S]methionine–[35S]cysteine mix and 0·8 mM-sodium laurate or oleate, the major fatty acids of CO and BT diets respectively. Concentrations of total [35S]proteins, [35S]albumin and [35S]ApoB in liver cells were 2-fold lower (P=0·08, 0·0004 and 0·03 respectively) in CO- than in BT-fed calves. Although the total amount of proteins secreted (including albumin) was similar in both groups of calves, the amount of VLDL-[35S]Apo secreted was 2-fold lower (P=0·004) in CO- than in BT-fed calves. These results suggest that a CO-enriched milk diet induces in preruminant calves a lipid infiltration of the liver by decreasing ApoB synthesis, leading to a reduction in secretion of VLDL particles.

Keywords

Liver slicesCoconut oilApolipoprotein BVLDLCalf
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 1 , July 2001 , pp. 13 - 19
Copyright
Copyright © The Nutrition Society 2001

References

Adeli, K, Mohammadi, A & Macri, J (1995) Regulation of apolipoprotein B biogenesis in human hepatocytes: posttranscriptional control mechanisms that determine the hepatic production of apolipoprotein B-containing lipoproteins. Clinical Biochemistry 28, 123–130.Google Scholar
Aurousseau, B, Thivend, P & Vermorel, M (1984) Influence du remplacement d'une partie du suif d'un aliment d'allaitement par de la tricaproïne ou de la tricapryline en association de l'huile de coprah sur la croissance du jeune veau préruminant (Effects on calf growth of substituting tricaproin or tricaprylin in association with coconut oil for a part of the tallow in a milk replacer for the preruminant calf). Annales de Zootechnie 33, 219–234.Google Scholar
Aurousseau, B, Vermorel, M & Bouvier, JC (1983) Influence du remplacement d'une partie du suif d'un aliment d'allaitement par de la tricaproïne ou de l'huile de coprah sur l'utilisation de l'énergie et de l'azote par le veau préruminant (Effects on energy and nitrogen balances of substituting tricaproin or coconut oil for a part of the tallow in a milk replacer for the preruminant calf). Reproduction Nutrition Développement 35, 167–178.Google Scholar
Bauchart, D & Aurousseau, B (1993) Digestion et métabolisme des lipides chez le veau de boucherie; conséquences sur la composition en lipides des tissus (Lipid digestion and metabolism in the veal calf; effects on the composition of tissue lipids). Viandes et Productions Carnées 14, 172–182.Google Scholar
Bauchart, D, Durand, D & Gruffat, D (1996) Lipid absorption and hepatic metabolism in ruminants. Proceedings of the Nutrition Society 55, 39–47.CrossRefGoogle ScholarPubMed
Bauchart, D, Durand, D, Gruffat-Mouty, D, Piot, C, Graulet, B, Chilliard, Y & Hocquette, JF (1999) Transport sanguin et métabolisme tissulaire des lipides chez le veau de boucherie (Blood transport and tissue metabolism of lipids in the preruminant calf). INRA Productions Animales 12, 273–285.CrossRefGoogle Scholar
Bauchart, D, Durand, D, Picherit, C, Graulet, B & Gruffat, D (1998) Effects of dietary coconut oil on blood transport and in vivo hepatic metabolism of fatty acids in the preruminant calf. Reproduction Nutrition Développement 38, 203.CrossRefGoogle Scholar
Bennett, AJ, Billett, MA, Salter, AM & White, DA (1995) Regulation of hamster hepatic triglyceride transfer protein mRNA levels by dietary fats. Biochemical and Biophysical Research Communications 212, 473–478.CrossRefGoogle ScholarPubMed
Bouma, ME, Beucler, I, Pessah, M, Heinzmann, C, Lusis, AJ, Naim, HY, Ducastelle, T, Leluyer, B, Schmitz, J, Infante, R & Aggerbeck, LP (1990) Description of two different patients with abetalipoproteinemia: synthesis of a normal-sized apolipoprotein B-48 in intestinal organ culture. Journal of Lipid Research 31, 1–15.CrossRefGoogle ScholarPubMed
Brett, DJ, Pease, RJ, Scott, J & Gibbons, GF (1995) Microsomal triglyceride transfer protein activity remains unchanged in rat livers under conditions of altered very-low-density lipoprotein secretion. Biochemical Journal 310, 11–14.CrossRefGoogle ScholarPubMed
Gibbons, GF (1990) Assembly and secretion of hepatic very-low-density lipoprotein. Biochemical Journal 268, 1–13.CrossRefGoogle ScholarPubMed
Gibbons, GF & Wiggins, D (1995) Intracellular triacylglycerol lipase: its role in the assembly of hepatic very-low-density lipoprotein (VLDL). Advances in Enzyme Regulation 35, 179–198.CrossRefGoogle ScholarPubMed
Graulet, B, Gruffat, D, Durand, D & Bauchart, D (1998) Fatty acid metabolism and very low density lipoprotein secretion in liver slices from rats and preruminant calves. Journal of Biochemistry 124, 1212–1219.CrossRefGoogle ScholarPubMed
Graulet, B, Gruffat-Mouty, D, Durand, D & Bauchart, D (2000) Effects of milk diets containing beef tallow or coconut oil on the fatty acid metabolism of liver slices from preruminant calves. British Journal of Nutrition 84, 309–318.CrossRefGoogle ScholarPubMed
Gruffat, D, Durand, D, Chilliard, Y, Williams, P & Bauchart, D (1996 a) Hepatic gene expression of apolipoprotein B100 during early lactation in underfed, high producing dairy cows. Journal of Dairy Science 80, 657–666.CrossRefGoogle Scholar
Gruffat, D, Durand, D, Graulet, B & Bauchart, D (1996 b) Regulation of VLDL synthesis and secretion in the liver. Reproduction Nutrition Développement 36, 375–389.Google Scholar
Gruffat-Mouty, D, Graulet, B, Durand, D, Samson-Bouma, ME & Bauchart, D (1999) Apolipoprotein B production and very low density lipoprotein secretion by calf liver slices. Journal of Biochemistry 126, 188–193.Google Scholar
Grummer, RR (1993) Etiology of lipid-related metabolic disorders in periparturient dairy cows. Journal of Dairy Science 76, 3882–3896.Google Scholar
Jenkins, KJ & Kramer, JKG (1986) Influence of low linolenic acid in milk replacer on calf performance and lipids in blood plasma, heart and liver. Journal of Dairy Science 69, 1374–1386.Google Scholar
Jenkins, KJ, Kramer, JKG & Sauer, FD (1985) Influence of triglycerides and free fatty acids in milk replacers on calf performance, blood plasma and adipose lipids. Journal of Dairy Science 68, 669–675.Google Scholar
Khosla, P & Hayes, KC (1993) Dietary palmitic acid raises plasma LDL cholesterol relative to oleic acid only at a high intake of cholesterol. Biochemica et Biophysica Acta 1210, 13–22.CrossRefGoogle Scholar
Labarca, C & Paigen, K (1980) A simple, rapid and sensitive DNA assay procedure. Analytical Biochemistry 102, 344–352.CrossRefGoogle ScholarPubMed
Lehner, R, Cui, Z & Vance, E (1999) Subcellular localisation, developmental expression and characterisation of a liver triacylglycerol hydrolase. Biochemical Journal 338, 761–768.Google Scholar
Lehner, R & Verger, R (1997) Purification and characterisation of a porcine microsomal triacylglycerol hydrolase. Biochemistry 36, 1861–1868.CrossRefGoogle ScholarPubMed
Leplaix-Charlat, L, Bauchart, D, Durand, D, Laplaud, PM & Chapman, MJ (1996 a) Plasma lipoproteins in preruminant calves fed diets containing tallow or soybean oil with and without cholesterol. Journal of Dairy Science 79, 1267–1277.Google Scholar
Leplaix-Charlat, L, Durand, D & Bauchart, D (1996 b) Effects of diets containing tallow and soybean oil with and without cholesterol on hepatic metabolism of lipids and lipoproteins in the preruminant calf. Journal of Dairy Science 79, 1826–1835.CrossRefGoogle ScholarPubMed
Marcos, E, Mazur, A, Cardot, P & Rayssiguier, Y (1990) The effect of pregnancy and lactation on serum lipid and apolipoprotein B and A-I levels in dairy cows. Journal of Animal Physiology and Animal Nutrition 64, 133–138.CrossRefGoogle Scholar
Petit, D, Raisonnier, A, Amit, N & Infante, R (1982) Lack of induction of VLDL apoprotein synthesis by medium chain fatty acids in the isolated rat liver. Annals in Nutrition and Metabolism 26, 279–286.Google Scholar
Reid, IM & Collins, RA (1980) The pathology of postparturient fatty liver in high-yielding dairy cows. Investigation in Cell Pathology 3, 237–249.Google ScholarPubMed
Sober, HA (1970) Handbook of Biochemistry. Selected Data of Molecular Biology, 2nd ed., Cleveland, OH: The Chemical Rubber Co.Google Scholar
Toullec, R (1978) Le Veau (The Calf) In Alimentation des Ruminants (Nutrition of Ruminants), pp. 245–274. Versailles Institut National de la Recherche Agronomique.Google Scholar
Ushida, E, Katoh, N & Takahashi, K (1992) Induction of fatty liver in cows by ethionine administration and concomitant decreases of serum apolipoproteins B-100 and A-I concentrations. American Journal of Veterinary Research 53, 2035–2042.Google Scholar
Wetterau, JR, Lin, MCM & Jamil, H (1997) Microsomal triglyceride transfer protein. Biochimica et Biophysica Acta 1345, 136–150.CrossRefGoogle ScholarPubMed
Yang, LY, Kuksis, A, Myher, JJ & Steiner, G (1995) Origin of triacylglycerol moiety of plasma very low density lipoproteins in the rat: structural studies. Journal of Lipid Research 36, 125–136.CrossRefGoogle ScholarPubMed
Yao, Z, Tran, K and McLeod RS (1997) Intracellular degradation of newly synthesized apolipoprotein B Journal of Lipid Research 38, 1937–1953.CrossRefGoogle ScholarPubMed
Yasuhara, M, Ohama, T, Matsuki, N, Saito, H, Matsushima, T, Kurokawa, K & Teramoto, BT (1991) Deficiency of apolipoprotein B synthesis in Suncus murinus. Journal of Biochemistry 110, 751–755.Google Scholar