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Effects of adrenalectomy before weaning in the genetically obese Zucker rat (fa/fa)

Published online by Cambridge University Press:  09 March 2007

J. M. Fletcher
J. M. Fletcher
Affiliation:
Rowett Research Institute, Bucksburn, AB2 9SB, Aberdeen
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Abstract

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1. Lean (Fa/?) and obese (fa/fa) Zucker rats were adrenalectomized or sham-operated at 19 d of age (3 d before weaning). Injection of corticosterone for 3 d after weaning (1.0 mg/d) was necessary to ensure survival of adrenalectomized fa/fa but not Fa/? rats. Intact and adrenalectomized fa/fa rats had a lower rectal temperature than Fa/? animals before and 3 d after adrenalectomy. The post-weaning survival of adrenalectomized fa/fa rats was enhanced by maintenance at an ambient temperature of 30° rather than 22°.

2. Adrenalectomized and sham-operated rats were therefore kept at 30°, fed ad-lib. and killed at 34 d. Adrenalectomy had only small effects on the growth, body composition and appetite of Fa/? rats. The hyperphagia, greater lipid content, reduced protein content and hyperinsulinaemia of fa/fa rats were completely abolished by adrenalectomy.

3. Intact fu/fa rats had higher liver glycogen contents and higher activities of the hepatic enzymes tyrosine aminotransferase (EC 2. 6. 1. 5) and acetyl CoA carboxylase (EC 6. 4. 1. 2) than intact Fa/? animals. Adrenalectomy abolished these phenotypic differences.

4. Injection of adrenalectomized rats with 1.0 mg corticosterone-21-acetate daily from weaning to 34 d restored the abnormal body composition, hyperphagia, hyperinsulinaemia, higher hepatic glycogen and enzyme activities of fa/fa rats.

5. In a second experiment adrenalectomized rats were injected with 1.0 mg corticosterone-21-acetate daily from weaning to 34 d and kept at 22°, fa/fu rats adrenalectomized and injected with corticosterone had a reduced body lipid content compared with intact fa/fa rats but still contained more lipid than intact or similarly treated Fa/? animals.

6. In both experiments adrenalectomized Fa/? and fa/fa rats injected daily with corticosterone had the same plasma concentrations of this hormone when killed 3 h after the last injection at 34 d. It is concluded that corticosterone is required for expression of the abnormal appetite, hyperinsulinaemia and body composition of the fa/fa rat.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 56 , Issue 1 , July 1986 , pp. 141 - 151
Copyright
Copyright © The Nutrition Society 1986

References

REFERENCES

Atkinson, T., Fowler, V. R., Garton, G. A. & Lough, A. K. (1972). Analyst, London 97, 562–568.CrossRefGoogle Scholar
Bell, G. E. & Stern, J. S. (1977). Growth 41, 63–80.Google Scholar
Bray, G. A. & York, D. A. (1979). Physiological Reviews 59, 719–809.CrossRefGoogle Scholar
Bray, G. A., York, D. A. & Sverdloff, R. S. (1973). Metabolism 22, 435–444.CrossRefGoogle Scholar
Cleary, M. P., Vasselli, J. R. & Greenwood, M. R. C. (1980). American Journal of Physiology 235, E254–E292.Google Scholar
Diamont, S. & Shafrir, E. (1975). European Journal of Biochemistry 53, 541–546.CrossRefGoogle Scholar
Dickson, A. J., Marston, F. A. O. & Pogson, C. I. (1981). FEBS Letters 127, 25–32.CrossRefGoogle Scholar
Fletcher, J. M., Haggarty, P., Wahle, K. W. J. & Reeds, P. J. (1983). Proceedings of the Nutrition Society 42, 44A.Google Scholar
Galpin, k. S., Henderson, R. G., James, W. P. T. & Trayhurn, P. (1983). Proceedings of the Nutrition Society 42, 159A.Google Scholar
Godbole, V., York, D. A. & Bloxham, D. B. (1978). Diabetologia 15, 41–44.CrossRefGoogle Scholar
Gosdow-Cohen, A., Chen, C. L. & Besch, E. L. (1982). Proceedings of the Society for Experimental Biological Medicine 70, 29–34.CrossRefGoogle Scholar
Granner, D. K. & Tomkins, G. M. (1970). Methods in Enzymology 17, 633–637.CrossRefGoogle Scholar
Hartree, E. F. (1972). Analytical Biochemistry 48, 422–427.CrossRefGoogle Scholar
Hausberger, F. X. & Hausberger, B. C. (1960). American Journal of Clinical Nutrition 8, 671–679.CrossRefGoogle Scholar
Holt, S. & York, D. A. (1982). Biochemical Journal 208, 819–822.CrossRefGoogle Scholar
Holt, S., York, D. A. & Fitzsimons, J. T. R. (1983). Biochemical Journal 214, 215–223.CrossRefGoogle Scholar
Huggett, A. StG. & Nixon, D.A. (1957). Lancet ii, 368–370.CrossRefGoogle Scholar
Inoue, H. & Lowenstein, I. M. (1975). Methods in Enzymology 35B, 3–11.CrossRefGoogle Scholar
Kenney, F. T. (1970). In Mammalian Protein Metabolism, vol. 4, pp. 131–176 [Munro, H. N., editor]. New York: Academic Press.CrossRefGoogle Scholar
Keppler, D. & Decker, K. (1984).In Methods of Enzymatic Analysis, vol. 4, 3rd ed., pp. 11–18 [Bergmeyer, H. U., editor]. Basal: Verlag Chemie.Google Scholar
Lin, E. C. C. & Knox, W. E. (1957). Biochimica et Biophysica Acta 26, 85–88.CrossRefGoogle Scholar
Loeb, J. N. (1976). New England Journal of Medicine 295, 47–552.CrossRefGoogle Scholar
Marchington, D., Rothwell, N. J., Stock, M. J. & York, D. A. (1983). Journal of Nutrition 113, 1395–1402.CrossRefGoogle Scholar
Martin, R. J. & Gahagan, J. (1977). Proceedings of the Society for Experimental Biology 154, 610–613.CrossRefGoogle Scholar
Pullar, J. D. & Webster, A. J. F. (1974). British Journal of Nutrition 31, 377–392.CrossRefGoogle Scholar
Reeds, P. J., Haggarty, P., Wahle, K. W. J. & Fletcher, J. M. (1982). Biochemical Journal 204, 393–398.CrossRefGoogle Scholar
Reineke, R. M. & Kendall, E. C. (1942). Endocrinology 31, 573–577.CrossRefGoogle Scholar
Saito, M. & Bray, G. A. (1984). American Journal of Physiology 246, R20–R25.Google Scholar
Shargill, N. S.York, D. A. & Marchington, D. R. (1983). Biochimica et Biophysica Acta 756, 297–307.CrossRefGoogle Scholar
Turkenkopf, I. J., Johnson, P. R. & Greenwood, M. R. C. (1982). American Journal of Physiology 242, E220–E225.Google Scholar
Volpe, J. J. & Vagelos, P. R. (1976). Physiological Reviews 56, 338–417.CrossRefGoogle Scholar
York, D. A. & Godbole, V. (1979) Hormone and Metabolic Research 11, 646.CrossRefGoogle Scholar
York, D. A., Shargill, N. S. & Godbole, V. (1981). Diabetologia 21, 143–148.CrossRefGoogle Scholar
Yukimura, Y. & Bray, G. A. (1970). Endocrine Research Communications 5, 159–198.Google Scholar
Yukimura, Y., Bray, G. A. & Wolfsen, A. R. (1978). Endocrinology 103, 1924–1928.CrossRefGoogle Scholar
Yukimura, Y., Parlow, A. & Bray, G. A. (1977). 59th Annual Meeting of the Endocrine Society, Chicago, Abst 556, p. 340.Google Scholar